Restriction map for
pF1KB3758
(Linear) (Six-base) MAPSORT of: KIBB3758.seq Check: 7614 from: 1 to: 1782
KIBB3758.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1353 1782
Size: 1353 429
AatII G_ACGT'C
Cuts at: 0 521 1782
Size: 521 1261
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1195 1782
Size: 1195 587
AfeI AGC'GCT
Cuts at: 0 1189 1782
Size: 1189 593
AlwNI CAG_nnn'CTG
Cuts at: 0 639 775 1173 1273 1659 1782
Size: 639 136 398 100 386 123
Fragments arranged by size:
639 398 386 136 123 100
ApaI G_GGCC'C
Cuts at: 0 1624 1740 1782
Size: 1624 116 42
Fragments arranged by size:
1624 116 42
ApaLI G'TGCA_C
Cuts at: 0 74 1782
Size: 74 1708
AvaI C'yCGr_G
Cuts at: 0 6 49 155 344 418 1782
Size: 6 43 106 189 74 1364
Fragments arranged by size:
1364 189 106 74 43 6
BamHI G'GATC_C
Cuts at: 0 151 1782
Size: 151 1631
BanI G'GyrC_C
Cuts at: 0 242 1449 1482 1782
Size: 242 1207 33 300
Fragments arranged by size:
1207 300 242 33
BanII G_rGCy'C
Cuts at: 0 110 204 240 700 755 1241 1624 1740
Size: 110 94 36 460 55 486 383 116
Cuts at: 1740 1782
Size: 42
Fragments arranged by size:
486 460 383 116 110 94 55 42
36
BbeI G_GCGC'C
Cuts at: 0 246 1782
Size: 246 1536
BbsI GAAGACnn'nnnn_
Cuts at: 0 394 521 863 1782
Size: 394 127 342 919
Fragments arranged by size:
919 394 342 127
BbvCI CC'TCA_GC
Cuts at: 0 87 961 1232 1382 1490 1696 1782
Size: 87 874 271 150 108 206 86
Fragments arranged by size:
874 271 206 150 108 87 86
BglI GCCn_nnn'nGGC
Cuts at: 0 217 1606 1782
Size: 217 1389 176
Fragments arranged by size:
1389 217 176
BlpI GC'TnA_GC
Cuts at: 0 699 1091 1782
Size: 699 392 691
Fragments arranged by size:
699 691 392
Bme1580I G_kGCm'C
Cuts at: 0 78 136 1454 1624 1729 1740 1782
Size: 78 58 1318 170 105 11 42
Fragments arranged by size:
1318 170 105 78 58 42 11
BmrI ACTGGGnnnn_n'
Cuts at: 0 168 1782
Size: 168 1614
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 242 481 562 742 991 1069 1579 1624
Size: 242 239 81 180 249 78 510 45
Cuts at: 1624 1636 1696 1782
Size: 12 60 86
Fragments arranged by size:
510 249 242 239 180 86 81 78
60 45 12
Bpu10I CC'TnA_GC
Cuts at: 0 87 434 890 961 1114 1232 1382 1490
Size: 87 347 456 71 153 118 150 108
Cuts at: 1490 1696 1782
Size: 206 86
Fragments arranged by size:
456 347 206 153 150 118 108 87
86 71
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1042 1782
Size: 1042 740
BsaI GGTCTCn'nnnn_
Cuts at: 0 1525 1782
Size: 1525 257
BsaHI Gr'CG_yC
Cuts at: 0 243 391 518 1782
Size: 243 148 127 1264
Fragments arranged by size:
1264 243 148 127
BsaWI w'CCGG_w
Cuts at: 0 102 196 278 1782
Size: 102 94 82 1504
Fragments arranged by size:
1504 102 94 82
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 24 559 682 724 745 796 817 820
Size: 24 535 123 42 21 51 21 3
Cuts at: 820 988 991 1249 1456 1474 1543 1561 1782
Size: 168 3 258 207 18 69 18 221
Fragments arranged by size:
535 258 221 207 168 123 69 51
42 24 21 21 18 18 3 3
BseYI C'CCAG_C
Cuts at: 0 57 693 1464 1541 1782
Size: 57 636 771 77 241
Fragments arranged by size:
771 636 241 77 57
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 901 1782
Size: 901 881
BsiHKAI G_wGCw'C
Cuts at: 0 78 204 755 873 1115 1241 1782
Size: 78 126 551 118 242 126 541
Fragments arranged by size:
551 541 242 126 126 118 78
BsmI GAATG_Cn'
Cuts at: 0 253 1782
Size: 253 1529
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1216 1253 1782
Size: 1216 37 529
Fragments arranged by size:
1216 529 37
Bsp1286I G_dGCh'C
Cuts at: 0 78 110 136 204 240 700 755 873
Size: 78 32 26 68 36 460 55 118
Cuts at: 873 1115 1241 1454 1624 1729 1740 1782
Size: 242 126 213 170 105 11 42
Fragments arranged by size:
460 242 213 170 126 118 105 78
68 55 42 36 32 26 11
BspEI T'CCGG_A
Cuts at: 0 102 1782
Size: 102 1680
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 869 1353 1782
Size: 869 484 429
Fragments arranged by size:
869 484 429
BsrBI CCG'CTC
Cuts at: 0 100 1782
Size: 100 1682
BsrDI GCAATG_nn'
Cuts at: 0 910 1782
Size: 910 872
BsrFI r'CCGG_y
Cuts at: 0 20 38 410 1485 1782
Size: 20 18 372 1075 297
Fragments arranged by size:
1075 372 297 20 18
BssHII G'CGCG_C
Cuts at: 0 438 1782
Size: 438 1344
BstXI CCAn_nnnn'nTGG
Cuts at: 0 563 1617 1782
Size: 563 1054 165
Fragments arranged by size:
1054 563 165
BstYI r'GATC_y
Cuts at: 0 151 1782
Size: 151 1631
Bsu36I CC'TnA_GG
Cuts at: 0 1327 1782
Size: 1327 455
BtgI C'CryG_G
Cuts at: 0 307 1731 1782
Size: 307 1424 51
Fragments arranged by size:
1424 307 51
BtsI GCAGTG_nn'
Cuts at: 0 614 871 927 1782
Size: 614 257 56 855
Fragments arranged by size:
855 614 257 56
DraIII CAC_nnn'GTG
Cuts at: 0 735 1782
Size: 735 1047
EaeI y'GGCC_r
Cuts at: 0 260 354 408 630 1661 1782
Size: 260 94 54 222 1031 121
Fragments arranged by size:
1031 260 222 121 94 54
EarI CTCTTCn'nnn_
Cuts at: 0 794 821 992 1211 1502 1782
Size: 794 27 171 219 291 280
Fragments arranged by size:
794 291 280 219 171 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 479 1782
Size: 479 1303
EcoICRI GAG'CTC
Cuts at: 0 202 753 1239 1782
Size: 202 551 486 543
Fragments arranged by size:
551 543 486 202
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 242 481 562 742 991 1069 1195 1579
Size: 242 239 81 180 249 78 126 384
Cuts at: 1579 1624 1636 1696 1782
Size: 45 12 60 86
Fragments arranged by size:
384 249 242 239 180 126 86 81
78 60 45 12
EcoO109I rG'GnC_Cy
Cuts at: 0 54 606 1621 1736 1737 1782
Size: 54 552 1015 115 1 45
Fragments arranged by size:
1015 552 115 54 45 1
EcoRV GAT'ATC
Cuts at: 0 1410 1782
Size: 1410 372
FspI TGC'GCA
Cuts at: 0 116 1782
Size: 116 1666
HaeII r_GCGC'y
Cuts at: 0 246 1191 1782
Size: 246 945 591
Fragments arranged by size:
945 591 246
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 265 297 1188 1220 1507 1539 1782
Size: 265 32 891 32 287 32 243
Fragments arranged by size:
891 287 265 243 32 32 32
KasI G'GCGC_C
Cuts at: 0 242 1782
Size: 242 1540
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1079 1782
Size: 1079 703
MscI TGG'CCA
Cuts at: 0 1663 1782
Size: 1663 119
MslI CAynn'nnrTG
Cuts at: 0 878 1782
Size: 878 904
MspA1I CmG'CkG
Cuts at: 0 376 939 990 1405 1613 1659 1695 1782
Size: 376 563 51 415 208 46 36 87
Fragments arranged by size:
563 415 376 208 87 51 46 36
NaeI GCC'GGC
Cuts at: 0 40 412 1487 1782
Size: 40 372 1075 295
Fragments arranged by size:
1075 372 295 40
NarI GG'CG_CC
Cuts at: 0 243 1782
Size: 243 1539
NgoMIV G'CCGG_C
Cuts at: 0 38 410 1485 1782
Size: 38 372 1075 297
Fragments arranged by size:
1075 372 297 38
PflMI CCAn_nnn'nTGG
Cuts at: 0 639 1086 1659 1782
Size: 639 447 573 123
Fragments arranged by size:
639 573 447 123
PfoI T'CCnGG_A
Cuts at: 0 1217 1782
Size: 1217 565
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1025 1057 1782
Size: 1025 32 725
Fragments arranged by size:
1025 725 32
PpuMI rG'GwC_Cy
Cuts at: 0 54 606 1782
Size: 54 552 1176
Fragments arranged by size:
1176 552 54
PspOMI G'GGCC_C
Cuts at: 0 1620 1736 1782
Size: 1620 116 46
Fragments arranged by size:
1620 116 46
PstI C_TGCA'G
Cuts at: 0 596 621 866 1163 1184 1768 1782
Size: 596 25 245 297 21 584 14
Fragments arranged by size:
596 584 297 245 25 21 14
PvuII CAG'CTG
Cuts at: 0 939 990 1659 1695 1782
Size: 939 51 669 36 87
Fragments arranged by size:
939 669 87 51 36
SacI G_AGCT'C
Cuts at: 0 204 755 1241 1782
Size: 204 551 486 541
Fragments arranged by size:
551 541 486 204
SanDI GG'GwC_CC
Cuts at: 0 54 1782
Size: 54 1728
SexAI A'CCwGG_T
Cuts at: 0 731 1782
Size: 731 1051
SfcI C'TryA_G
Cuts at: 0 592 617 862 1159 1180 1344 1764 1782
Size: 592 25 245 297 21 164 420 18
Fragments arranged by size:
592 420 297 245 164 25 21 18
SfoI GGC'GCC
Cuts at: 0 244 1782
Size: 244 1538
SmaI CCC'GGG
Cuts at: 0 51 346 420 1782
Size: 51 295 74 1362
Fragments arranged by size:
1362 295 74 51
SmlI C'TyrA_G
Cuts at: 0 1021 1782
Size: 1021 761
StuI AGG'CCT
Cuts at: 0 1003 1782
Size: 1003 779
StyI C'CwwG_G
Cuts at: 0 227 267 327 704 1782
Size: 227 40 60 377 1078
Fragments arranged by size:
1078 377 227 60 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 523 1549 1782
Size: 523 1026 233
Fragments arranged by size:
1026 523 233
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 292 324 457 489 1782
Size: 292 32 133 32 1293
Fragments arranged by size:
1293 292 133 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 334 1083 1782
Size: 334 749 699
Fragments arranged by size:
749 699 334
XmaI C'CCGG_G
Cuts at: 0 49 344 418 1782
Size: 49 295 74 1364
Fragments arranged by size:
1364 295 74 49
ZraI GAC'GTC
Cuts at: 0 519 1782
Size: 519 1263
Enzymes that do cut:
AarI AatII AcuI AfeI AlwNI ApaI ApaLI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BglI BlpI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspMI BsrBI BsrDI BsrFI BssHII BstXI BstYI Bsu36I
BtgI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRV FspI HaeII Hin4I KasI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV PflMI PfoI PpiI
PpuMI PspOMI PstI PvuII SacI SanDI SexAI SfcI
SfoI SmaI SmlI StuI StyI TaqII TstI XcmI
XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AflIII AgeI AhdI AleI
AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI
BcgI BciVI BclI BfrBI BglII BmgBI BmtI BplI
BsaAI BsaBI BsaXI BsiEI BsiWI BspHI BsrGI BssSI
BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI
DrdI EagI EcoNI EcoRI FalI FseI FspAI HincII
HindIII HpaI KpnI MfeI MluI NcoI NdeI NheI
NotI NruI NsiI NspI PacI PasI PciI PmeI
PmlI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SapI SbfI ScaI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI
XhoI XmnI