Restriction map for
pF1KB3767
(Linear) (Six-base) MAPSORT of: KIBB3767.seq Check: 3489 from: 1 to: 3279
KIBB3767.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AccI GT'mk_AC
Cuts at: 0 640 1931 3279
Size: 640 1291 1348
Fragments arranged by size:
1348 1291 640
Acc65I G'GTAC_C
Cuts at: 0 2594 3279
Size: 2594 685
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 273 539 1139 1151 1909 2221 3074 3279
Size: 273 266 600 12 758 312 853 205
Fragments arranged by size:
853 758 600 312 273 266 205 12
AlwNI CAG_nnn'CTG
Cuts at: 0 48 1707 2277 3279
Size: 48 1659 570 1002
Fragments arranged by size:
1659 1002 570 48
ApoI r'AATT_y
Cuts at: 0 282 1354 1884 2328 2474 2502 3279
Size: 282 1072 530 444 146 28 777
Fragments arranged by size:
1072 777 530 444 282 146 28
AvaI C'yCGr_G
Cuts at: 0 822 3279
Size: 822 2457
BamHI G'GATC_C
Cuts at: 0 2347 3279
Size: 2347 932
BanI G'GyrC_C
Cuts at: 0 564 2594 3279
Size: 564 2030 685
Fragments arranged by size:
2030 685 564
BanII G_rGCy'C
Cuts at: 0 814 823 3279
Size: 814 9 2456
Fragments arranged by size:
2456 814 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 710 847 1915 2477 3279
Size: 710 137 1068 562 802
Fragments arranged by size:
1068 802 710 562 137
BciVI GTATCCnnnnn_n'
Cuts at: 0 165 3279
Size: 165 3114
BclI T'GATC_A
Cuts at: 0 2871 3279
Size: 2871 408
BglII A'GATC_T
Cuts at: 0 1911 3213 3279
Size: 1911 1302 66
Fragments arranged by size:
1911 1302 66
BlpI GC'TnA_GC
Cuts at: 0 38 813 1283 3279
Size: 38 775 470 1996
Fragments arranged by size:
1996 775 470 38
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 12 338 2233 2596 3279
Size: 12 326 1895 363 683
Fragments arranged by size:
1895 683 363 326 12
Bpu10I CC'TnA_GC
Cuts at: 0 2983 3279
Size: 2983 296
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2720 2787 3279
Size: 2720 67 492
Fragments arranged by size:
2720 492 67
BsaI GGTCTCn'nnnn_
Cuts at: 0 112 3279
Size: 112 3167
BsaBI GATnn'nnATC
Cuts at: 0 3143 3279
Size: 3143 136
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 711 741 1002 1032 1375 1405 3279
Size: 711 30 261 30 343 30 1874
Fragments arranged by size:
1874 711 343 261 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 112 719 1819 3279
Size: 112 607 1100 1460
Fragments arranged by size:
1460 1100 607 112
BseYI C'CCAG_C
Cuts at: 0 20 2540 3279
Size: 20 2520 739
Fragments arranged by size:
2520 739 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1693 2465 3279
Size: 1693 772 814
Fragments arranged by size:
1693 814 772
BsiHKAI G_wGCw'C
Cuts at: 0 814 823 3279
Size: 814 9 2456
Fragments arranged by size:
2456 814 9
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2641 3279
Size: 2641 638
Bsp1286I G_dGCh'C
Cuts at: 0 814 823 3279
Size: 814 9 2456
Fragments arranged by size:
2456 814 9
BspHI T'CATG_A
Cuts at: 0 789 2868 3279
Size: 789 2079 411
Fragments arranged by size:
2079 789 411
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 487 1305 3279
Size: 487 818 1974
Fragments arranged by size:
1974 818 487
BsrDI GCAATG_nn'
Cuts at: 0 2170 2308 3279
Size: 2170 138 971
Fragments arranged by size:
2170 971 138
BsrGI T'GTAC_A
Cuts at: 0 990 3236 3279
Size: 990 2246 43
Fragments arranged by size:
2246 990 43
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2151 3279
Size: 2151 1128
BstYI r'GATC_y
Cuts at: 0 1661 1911 2347 3213 3279
Size: 1661 250 436 866 66
Fragments arranged by size:
1661 866 436 250 66
Bsu36I CC'TnA_GG
Cuts at: 0 686 2161 3279
Size: 686 1475 1118
Fragments arranged by size:
1475 1118 686
BtgI C'CryG_G
Cuts at: 0 1889 2489 3279
Size: 1889 600 790
Fragments arranged by size:
1889 790 600
BtsI GCAGTG_nn'
Cuts at: 0 1294 3279
Size: 1294 1985
EaeI y'GGCC_r
Cuts at: 0 417 1917 3279
Size: 417 1500 1362
Fragments arranged by size:
1500 1362 417
EarI CTCTTCn'nnn_
Cuts at: 0 89 325 468 1796 1838 2639 3089 3185
Size: 89 236 143 1328 42 801 450 96
Cuts at: 3185 3279
Size: 94
Fragments arranged by size:
1328 801 450 236 143 96 94 89
42
EcoICRI GAG'CTC
Cuts at: 0 812 821 3279
Size: 812 9 2458
Fragments arranged by size:
2458 812 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 12 273 338 539 1139 1151 1909 2221
Size: 12 261 65 201 600 12 758 312
Cuts at: 2221 2233 2596 3074 3279
Size: 12 363 478 205
Fragments arranged by size:
758 600 478 363 312 261 205 201
65 12 12 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 52 3279
Size: 52 3227
EcoO109I rG'GnC_Cy
Cuts at: 0 45 1827 2157 3279
Size: 45 1782 330 1122
Fragments arranged by size:
1782 1122 330 45
EcoRI G'AATT_C
Cuts at: 0 2502 3279
Size: 2502 777
EcoRV GAT'ATC
Cuts at: 0 1227 3279
Size: 1227 2052
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1618 2101 2479 3279
Size: 1618 483 378 800
Fragments arranged by size:
1618 800 483 378
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 832 864 1027 1059 3200 3232 3257 3279
Size: 832 32 163 32 2141 32 25 22
Fragments arranged by size:
2141 832 163 32 32 32 25 22
HincII GTy'rAC
Cuts at: 0 1209 3279
Size: 1209 2070
HindIII A'AGCT_T
Cuts at: 0 1430 3279
Size: 1430 1849
HpaI GTT'AAC
Cuts at: 0 1209 3279
Size: 1209 2070
KpnI G_GTAC'C
Cuts at: 0 2598 3279
Size: 2598 681
MscI TGG'CCA
Cuts at: 0 419 3279
Size: 419 2860
MslI CAynn'nnrTG
Cuts at: 0 794 2149 2361 3279
Size: 794 1355 212 918
Fragments arranged by size:
1355 918 794 212
MspA1I CmG'CkG
Cuts at: 0 1707 3279
Size: 1707 1572
NcoI C'CATG_G
Cuts at: 0 1889 2489 3279
Size: 1889 600 790
Fragments arranged by size:
1889 790 600
PasI CC'CwG_GG
Cuts at: 0 2343 3279
Size: 2343 936
PfoI T'CCnGG_A
Cuts at: 0 89 2249 3279
Size: 89 2160 1030
Fragments arranged by size:
2160 1030 89
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 936 968 3279
Size: 936 32 2311
Fragments arranged by size:
2311 936 32
PpuMI rG'GwC_Cy
Cuts at: 0 1827 2157 3279
Size: 1827 330 1122
Fragments arranged by size:
1827 1122 330
PshAI GACnn'nnGTC
Cuts at: 0 265 3279
Size: 265 3014
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2578 2610 3279
Size: 2578 32 669
Fragments arranged by size:
2578 669 32
PstI C_TGCA'G
Cuts at: 0 1635 2094 3170 3279
Size: 1635 459 1076 109
Fragments arranged by size:
1635 1076 459 109
PvuII CAG'CTG
Cuts at: 0 1707 3279
Size: 1707 1572
SacI G_AGCT'C
Cuts at: 0 814 823 3279
Size: 814 9 2456
Fragments arranged by size:
2456 814 9
SexAI A'CCwGG_T
Cuts at: 0 2597 3279
Size: 2597 682
SfcI C'TryA_G
Cuts at: 0 836 1631 2090 2450 2836 2965 3166 3279
Size: 836 795 459 360 386 129 201 113
Fragments arranged by size:
836 795 459 386 360 201 129 113
SmlI C'TyrA_G
Cuts at: 0 822 2735 2802 3279
Size: 822 1913 67 477
Fragments arranged by size:
1913 822 477 67
SpeI A'CTAG_T
Cuts at: 0 3060 3279
Size: 3060 219
SspI AAT'ATT
Cuts at: 0 1872 3279
Size: 1872 1407
StyI C'CwwG_G
Cuts at: 0 1889 2489 3279
Size: 1889 600 790
Fragments arranged by size:
1889 790 600
TatI w'GTAC_w
Cuts at: 0 990 3236 3279
Size: 990 2246 43
Fragments arranged by size:
2246 990 43
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1002 1034 1250 1282 3279
Size: 1002 32 216 32 1997
Fragments arranged by size:
1997 1002 216 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2358 3269 3279
Size: 2358 911 10
Fragments arranged by size:
2358 911 10
XbaI T'CTAG_A
Cuts at: 0 1422 3027 3279
Size: 1422 1605 252
Fragments arranged by size:
1605 1422 252
XhoI C'TCGA_G
Cuts at: 0 822 3279
Size: 822 2457
XmnI GAAnn'nnTTC
Cuts at: 0 147 3203 3279
Size: 147 3056 76
Fragments arranged by size:
3056 147 76
Enzymes that do cut:
AccI Acc65I AcuI AlwNI ApoI AvaI BamHI BanI
BanII BbsI BciVI BclI BglII BlpI BpmI Bpu10I
BpuEI BsaI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrDI BsrGI BstXI BstYI
Bsu36I BtgI BtsI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI Hin4I HincII HindIII HpaI
KpnI MscI MslI MspA1I NcoI PasI PfoI PpiI
PpuMI PshAI PsrI PstI PvuII SacI SexAI SfcI
SmlI SpeI SspI StyI TatI TstI Tth111I XbaI
XhoI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BbeI BbvCI BcgI BfrBI BglI Bme1580I
BmgBI BmrI BmtI BplI BsaAI BsaHI BsaWI BsiEI
BsiWI BsmI BspEI BsrBI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EciI FseI FspI FspAI HaeII KasI
MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PflMI PmeI
PmlI PsiI PspOMI PspXI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SphI SrfI StuI SwaI TaqII XcmI
XmaI ZraI