Restriction map for
pF1KB3783
(Linear) (Six-base) MAPSORT of: KIBB3783.seq Check: 2311 from: 1 to: 2274
KIBB3783.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AatII G_ACGT'C
Cuts at: 0 1610 2018 2274
Size: 1610 408 256
Fragments arranged by size:
1610 408 256
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 649 1693 1843 2274
Size: 280 369 1044 150 431
Fragments arranged by size:
1044 431 369 280 150
AflII C'TTAA_G
Cuts at: 0 352 2274
Size: 352 1922
AhdI GACnn_n'nnGTC
Cuts at: 0 801 2019 2274
Size: 801 1218 255
Fragments arranged by size:
1218 801 255
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 544 576 2274
Size: 544 32 1698
Fragments arranged by size:
1698 544 32
AlwNI CAG_nnn'CTG
Cuts at: 0 18 2055 2274
Size: 18 2037 219
Fragments arranged by size:
2037 219 18
ApaI G_GGCC'C
Cuts at: 0 98 2274
Size: 98 2176
ApaLI G'TGCA_C
Cuts at: 0 1614 2137 2274
Size: 1614 523 137
Fragments arranged by size:
1614 523 137
AvaI C'yCGr_G
Cuts at: 0 757 1123 1167 2274
Size: 757 366 44 1107
Fragments arranged by size:
1107 757 366 44
BamHI G'GATC_C
Cuts at: 0 163 277 1868 2274
Size: 163 114 1591 406
Fragments arranged by size:
1591 406 163 114
BanI G'GyrC_C
Cuts at: 0 753 2274
Size: 753 1521
BanII G_rGCy'C
Cuts at: 0 98 951 1235 1348 1556 1714 2213 2274
Size: 98 853 284 113 208 158 499 61
Fragments arranged by size:
853 499 284 208 158 113 98 61
BbeI G_GCGC'C
Cuts at: 0 757 2274
Size: 757 1517
BbsI GAAGACnn'nnnn_
Cuts at: 0 113 2274
Size: 113 2161
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2052 2086 2274
Size: 2052 34 188
Fragments arranged by size:
2052 188 34
BglII A'GATC_T
Cuts at: 0 624 1245 2274
Size: 624 621 1029
Fragments arranged by size:
1029 624 621
BlpI GC'TnA_GC
Cuts at: 0 572 2122 2234 2274
Size: 572 1550 112 40
Fragments arranged by size:
1550 572 112 40
Bme1580I G_kGCm'C
Cuts at: 0 98 665 1618 2141 2274
Size: 98 567 953 523 133
Fragments arranged by size:
953 567 523 133 98
BmgBI CAC'GTC
Cuts at: 0 2136 2261 2274
Size: 2136 125 13
Fragments arranged by size:
2136 125 13
BmrI ACTGGGnnnn_n'
Cuts at: 0 240 1255 1995 2274
Size: 240 1015 740 279
Fragments arranged by size:
1015 740 279 240
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 526 2274
Size: 526 1748
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1878 2227 2274
Size: 1878 349 47
Fragments arranged by size:
1878 349 47
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1200 1474 2274
Size: 1200 274 800
Fragments arranged by size:
1200 800 274
BsaI GGTCTCn'nnnn_
Cuts at: 0 55 865 1421 1661 2274
Size: 55 810 556 240 613
Fragments arranged by size:
810 613 556 240 55
BsaAI yAC'GTr
Cuts at: 0 2037 2274
Size: 2037 237
BsaBI GATnn'nnATC
Cuts at: 0 199 1447 2274
Size: 199 1248 827
Fragments arranged by size:
1248 827 199
BsaHI Gr'CG_yC
Cuts at: 0 182 754 1141 1160 1607 2015 2274
Size: 182 572 387 19 447 408 259
Fragments arranged by size:
572 447 408 387 259 182 19
BsaWI w'CCGG_w
Cuts at: 0 1055 2274
Size: 1055 1219
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1137 1167 2274
Size: 1137 30 1107
Fragments arranged by size:
1137 1107 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 85 562 1948 2274
Size: 85 477 1386 326
Fragments arranged by size:
1386 477 326 85
BseYI C'CCAG_C
Cuts at: 0 325 900 978 2274
Size: 325 575 78 1296
Fragments arranged by size:
1296 575 325 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1234 1297 2274
Size: 1234 63 977
Fragments arranged by size:
1234 977 63
BsiEI CG_ry'CG
Cuts at: 0 743 2274
Size: 743 1531
BsiHKAI G_wGCw'C
Cuts at: 0 818 951 1556 1618 2075 2141 2274
Size: 818 133 605 62 457 66 133
Fragments arranged by size:
818 605 457 133 133 66 62
Bsp1286I G_dGCh'C
Cuts at: 0 98 665 818 951 1235 1348 1556 1618
Size: 98 567 153 133 284 113 208 62
Cuts at: 1618 1714 2075 2141 2213 2274
Size: 96 361 66 72 61
Fragments arranged by size:
567 361 284 208 153 133 113 98
96 72 66 62 61
BspHI T'CATG_A
Cuts at: 0 1778 2274
Size: 1778 496
BsrFI r'CCGG_y
Cuts at: 0 770 974 2274
Size: 770 204 1300
Fragments arranged by size:
1300 770 204
BsrGI T'GTAC_A
Cuts at: 0 1691 1877 2274
Size: 1691 186 397
Fragments arranged by size:
1691 397 186
BssHII G'CGCG_C
Cuts at: 0 1758 2274
Size: 1758 516
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1248 2274
Size: 1248 1026
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1632 1806 2274
Size: 1632 174 468
Fragments arranged by size:
1632 468 174
BstYI r'GATC_y
Cuts at: 0 75 163 237 277 624 1245 1448 1868
Size: 75 88 74 40 347 621 203 420
Cuts at: 1868 2274
Size: 406
Fragments arranged by size:
621 420 406 347 203 88 75 74
40
Bsu36I CC'TnA_GG
Cuts at: 0 1100 1931 2192 2274
Size: 1100 831 261 82
Fragments arranged by size:
1100 831 261 82
BtgI C'CryG_G
Cuts at: 0 875 1382 1631 2083 2200 2274
Size: 875 507 249 452 117 74
Fragments arranged by size:
875 507 452 249 117 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1380 2077 2170 2274
Size: 1380 697 93 104
Fragments arranged by size:
1380 697 104 93
BtsI GCAGTG_nn'
Cuts at: 0 110 1094 1962 2274
Size: 110 984 868 312
Fragments arranged by size:
984 868 312 110
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2154 2189 2274
Size: 2154 35 85
Fragments arranged by size:
2154 85 35
DraIII CAC_nnn'GTG
Cuts at: 0 2145 2274
Size: 2145 129
EaeI y'GGCC_r
Cuts at: 0 1464 1796 2159 2274
Size: 1464 332 363 115
Fragments arranged by size:
1464 363 332 115
EarI CTCTTCn'nnn_
Cuts at: 0 517 650 958 1433 2245 2274
Size: 517 133 308 475 812 29
Fragments arranged by size:
812 517 475 308 133 29
EcoICRI GAG'CTC
Cuts at: 0 949 1554 2274
Size: 949 605 720
Fragments arranged by size:
949 720 605
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 649 1693 1843 1878 2227 2274
Size: 280 369 1044 150 35 349 47
Fragments arranged by size:
1044 369 349 280 150 47 35
EcoNI CCTnn'n_nnAGG
Cuts at: 0 691 1225 2146 2274
Size: 691 534 921 128
Fragments arranged by size:
921 691 534 128
EcoO109I rG'GnC_Cy
Cuts at: 0 94 348 507 839 1818 2196 2274
Size: 94 254 159 332 979 378 78
Fragments arranged by size:
979 378 332 254 159 94 78
EcoRV GAT'ATC
Cuts at: 0 1997 2274
Size: 1997 277
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 240 2274
Size: 240 2034
HaeII r_GCGC'y
Cuts at: 0 757 1017 2055 2274
Size: 757 260 1038 219
Fragments arranged by size:
1038 757 260 219
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 787 819 864 896 1981 2005 2013 2037
Size: 787 32 45 32 1085 24 8 24
Cuts at: 2037 2092 2124 2274
Size: 55 32 150
Fragments arranged by size:
1085 787 150 55 45 32 32 32
24 24 8
HincII GTy'rAC
Cuts at: 0 2094 2274
Size: 2094 180
HindIII A'AGCT_T
Cuts at: 0 442 2274
Size: 442 1832
KasI G'GCGC_C
Cuts at: 0 753 2274
Size: 753 1521
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 344 2249 2274
Size: 344 1905 25
Fragments arranged by size:
1905 344 25
MslI CAynn'nnrTG
Cuts at: 0 1339 2274
Size: 1339 935
MspA1I CmG'CkG
Cuts at: 0 139 1755 2202 2274
Size: 139 1616 447 72
Fragments arranged by size:
1616 447 139 72
NarI GG'CG_CC
Cuts at: 0 754 2274
Size: 754 1520
NcoI C'CATG_G
Cuts at: 0 875 1631 2274
Size: 875 756 643
Fragments arranged by size:
875 756 643
PasI CC'CwG_GG
Cuts at: 0 1650 2274
Size: 1650 624
PflMI CCAn_nnn'nTGG
Cuts at: 0 606 1040 2274
Size: 606 434 1234
Fragments arranged by size:
1234 606 434
PfoI T'CCnGG_A
Cuts at: 0 485 647 1226 1556 2274
Size: 485 162 579 330 718
Fragments arranged by size:
718 579 485 330 162
PmlI CAC'GTG
Cuts at: 0 2037 2274
Size: 2037 237
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 523 555 2274
Size: 523 32 1719
Fragments arranged by size:
1719 523 32
PpuMI rG'GwC_Cy
Cuts at: 0 348 507 839 1818 2274
Size: 348 159 332 979 456
Fragments arranged by size:
979 456 348 332 159
PshAI GACnn'nnGTC
Cuts at: 0 2259 2274
Size: 2259 15
PspOMI G'GGCC_C
Cuts at: 0 94 2274
Size: 94 2180
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1036 1068 2274
Size: 1036 32 1206
Fragments arranged by size:
1206 1036 32
PstI C_TGCA'G
Cuts at: 0 117 2274
Size: 117 2157
PvuI CG_AT'CG
Cuts at: 0 743 2274
Size: 743 1531
PvuII CAG'CTG
Cuts at: 0 139 1755 2274
Size: 139 1616 519
Fragments arranged by size:
1616 519 139
SacI G_AGCT'C
Cuts at: 0 951 1556 2274
Size: 951 605 718
Fragments arranged by size:
951 718 605
SacII CC_GC'GG
Cuts at: 0 2203 2274
Size: 2203 71
SanDI GG'GwC_CC
Cuts at: 0 839 2274
Size: 839 1435
SexAI A'CCwGG_T
Cuts at: 0 1523 2141 2274
Size: 1523 618 133
Fragments arranged by size:
1523 618 133
SfcI C'TryA_G
Cuts at: 0 113 618 2274
Size: 113 505 1656
Fragments arranged by size:
1656 505 113
SfoI GGC'GCC
Cuts at: 0 755 2274
Size: 755 1519
SmlI C'TyrA_G
Cuts at: 0 352 1215 1489 2274
Size: 352 863 274 785
Fragments arranged by size:
863 785 352 274
StuI AGG'CCT
Cuts at: 0 1098 2274
Size: 1098 1176
StyI C'CwwG_G
Cuts at: 0 581 875 1631 2274
Size: 581 294 756 643
Fragments arranged by size:
756 643 581 294
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 395 970 1283 2076 2274
Size: 395 575 313 793 198
Fragments arranged by size:
793 575 395 313 198
TatI w'GTAC_w
Cuts at: 0 746 1691 1877 2274
Size: 746 945 186 397
Fragments arranged by size:
945 746 397 186
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1137 1169 1941 1973 2274
Size: 1137 32 772 32 301
Fragments arranged by size:
1137 772 301 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1309 2274
Size: 1309 965
ZraI GAC'GTC
Cuts at: 0 1608 2016 2274
Size: 1608 408 258
Fragments arranged by size:
1608 408 258
Enzymes that do cut:
AatII AcuI AflII AhdI AloI AlwNI ApaI ApaLI
AvaI BamHI BanI BanII BbeI BbsI BcgI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspHI BsrFI BsrGI BssHII BstAPI
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI
HaeII Hin4I HincII HindIII KasI MmeI MslI MspA1I
NarI NcoI PasI PflMI PfoI PmlI PpiI PpuMI
PshAI PspOMI PsrI PstI PvuI PvuII SacI SacII
SanDI SexAI SfcI SfoI SmlI StuI StyI TaqII
TatI TstI XcmI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflIII AgeI AleI
AlfI ApoI AscI AseI AsiSI AvrII BaeI BbvCI
BciVI BclI BfrBI BglI BmtI Bpu10I BsiWI BsmI
BsmBI BspEI BspMI BsrBI BsrDI BssSI BstBI BstEII
BstZ17I ClaI DraI DrdI EagI EciI EcoRI FseI
FspI FspAI HpaI KpnI MfeI MluI MscI NaeI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PmeI PsiI PspXI RsrII SalI SapI SbfI
ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XbaI XhoI XmaI XmnI