Restriction map for
pF1KB3788
(Linear) (Six-base) MAPSORT of: KIBB3788.seq Check: 5442 from: 1 to: 2142
KIBB3788.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AccI GT'mk_AC
Cuts at: 0 1240 2142
Size: 1240 902
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 353 568 876 1731 2142
Size: 353 215 308 855 411
Fragments arranged by size:
855 411 353 308 215
AlwNI CAG_nnn'CTG
Cuts at: 0 72 2142
Size: 72 2070
ApoI r'AATT_y
Cuts at: 0 214 359 426 535 738 823 1306 1404
Size: 214 145 67 109 203 85 483 98
Cuts at: 1404 1566 1804 1844 2059 2096 2142
Size: 162 238 40 215 37 46
Fragments arranged by size:
483 238 215 214 203 162 145 109
98 85 67 46 40 37
AseI AT'TA_AT
Cuts at: 0 448 2142
Size: 448 1694
AvaI C'yCGr_G
Cuts at: 0 1316 2142
Size: 1316 826
BanI G'GyrC_C
Cuts at: 0 1583 2142
Size: 1583 559
BanII G_rGCy'C
Cuts at: 0 846 2142
Size: 846 1296
BbsI GAAGACnn'nnnn_
Cuts at: 0 290 1884 2142
Size: 290 1594 258
Fragments arranged by size:
1594 290 258
BciVI GTATCCnnnnn_n'
Cuts at: 0 546 2142
Size: 546 1596
BfrBI ATG'CAT
Cuts at: 0 629 748 2142
Size: 629 119 1394
Fragments arranged by size:
1394 629 119
Bme1580I G_kGCm'C
Cuts at: 0 1586 2142
Size: 1586 556
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 264 2142
Size: 264 1878
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 73 205 952 2142
Size: 73 132 747 1190
Fragments arranged by size:
1190 747 132 73
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1069 2142
Size: 1069 1073
BsmI GAATG_Cn'
Cuts at: 0 631 790 2142
Size: 631 159 1352
Fragments arranged by size:
1352 631 159
Bsp1286I G_dGCh'C
Cuts at: 0 846 1586 2142
Size: 846 740 556
Fragments arranged by size:
846 740 556
BsrDI GCAATG_nn'
Cuts at: 0 7 2142
Size: 7 2135
BsrGI T'GTAC_A
Cuts at: 0 915 2001 2142
Size: 915 1086 141
Fragments arranged by size:
1086 915 141
BstXI CCAn_nnnn'nTGG
Cuts at: 0 242 2142
Size: 242 1900
BtgI C'CryG_G
Cuts at: 0 196 2142
Size: 196 1946
BtsI GCAGTG_nn'
Cuts at: 0 795 2142
Size: 795 1347
DraI TTT'AAA
Cuts at: 0 1403 2142
Size: 1403 739
EarI CTCTTCn'nnn_
Cuts at: 0 329 1352 1436 1514 1682 1750 2100 2142
Size: 329 1023 84 78 168 68 350 42
Fragments arranged by size:
1023 350 329 168 84 78 68 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 264 353 568 876 1731 2142
Size: 264 89 215 308 855 411
Fragments arranged by size:
855 411 308 264 215 89
EcoO109I rG'GnC_Cy
Cuts at: 0 1112 2142
Size: 1112 1030
EcoRI G'AATT_C
Cuts at: 0 1844 2096 2142
Size: 1844 252 46
Fragments arranged by size:
1844 252 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1101 1133 1239 1271 2142
Size: 1101 32 106 32 871
Fragments arranged by size:
1101 871 106 32 32
HincII GTy'rAC
Cuts at: 0 226 2142
Size: 226 1916
HindIII A'AGCT_T
Cuts at: 0 1195 2142
Size: 1195 947
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 158 2142
Size: 92 66 1984
Fragments arranged by size:
1984 92 66
MslI CAynn'nnrTG
Cuts at: 0 407 730 2142
Size: 407 323 1412
Fragments arranged by size:
1412 407 323
MspA1I CmG'CkG
Cuts at: 0 72 2142
Size: 72 2070
NsiI A_TGCA'T
Cuts at: 0 631 750 2142
Size: 631 119 1392
Fragments arranged by size:
1392 631 119
PsiI TTA'TAA
Cuts at: 0 1202 2142
Size: 1202 940
PvuII CAG'CTG
Cuts at: 0 72 2142
Size: 72 2070
SfcI C'TryA_G
Cuts at: 0 120 1923 2007 2142
Size: 120 1803 84 135
Fragments arranged by size:
1803 135 120 84
SspI AAT'ATT
Cuts at: 0 579 2142
Size: 579 1563
SwaI ATTT'AAAT
Cuts at: 0 1403 2142
Size: 1403 739
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 67 2142
Size: 67 2075
TatI w'GTAC_w
Cuts at: 0 915 1431 1761 2001 2121 2142
Size: 915 516 330 240 120 21
Fragments arranged by size:
915 516 330 240 120 21
XmnI GAAnn'nnTTC
Cuts at: 0 578 889 1014 1057 1141 1555 1680 1848
Size: 578 311 125 43 84 414 125 168
Cuts at: 1848 1932 2016 2059 2142
Size: 84 84 43 83
Fragments arranged by size:
578 414 311 168 125 125 84 84
84 83 43 43
Enzymes that do cut:
AccI AcuI AlwNI ApoI AseI AvaI BanI BanII
BbsI BciVI BfrBI Bme1580I BpmI BseRI BsgI BsmI
Bsp1286I BsrDI BsrGI BstXI BtgI BtsI DraI EarI
Eco57MI EcoO109I EcoRI Hin4I HincII HindIII MmeI MslI
MspA1I NsiI PsiI PvuII SfcI SspI SwaI TaqII
TatI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AsiSI
AvrII BaeI BamHI BbeI BbvCI BcgI BclI BglI
BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI
BsiHKAI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgZI ClaI CspCI DraIII DrdI EaeI EagI EciI
EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HaeII
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI StuI StyI TstI Tth111I XbaI XcmI XhoI
XmaI ZraI