Restriction map for
pF1KB3789
(Linear) (Six-base) MAPSORT of: KIBB3789.seq Check: 6596 from: 1 to: 4593
KIBB3789.orf
With 182 enzymes: SgfI *
February 8, 2013 18:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2490 4593
Size: 2490 2103
AatII G_ACGT'C
Cuts at: 0 2510 4204 4593
Size: 2510 1694 389
Fragments arranged by size:
2510 1694 389
AccI GT'mk_AC
Cuts at: 0 530 1700 4593
Size: 530 1170 2893
Fragments arranged by size:
2893 1170 530
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 386 1627 1636 2470 3439 3559 4243 4593
Size: 386 1241 9 834 969 120 684 350
Fragments arranged by size:
1241 969 834 684 386 350 120 9
AfeI AGC'GCT
Cuts at: 0 636 3517 4593
Size: 636 2881 1076
Fragments arranged by size:
2881 1076 636
AflIII A'CryG_T
Cuts at: 0 743 4593
Size: 743 3850
AhdI GACnn_n'nnGTC
Cuts at: 0 723 1900 4233 4593
Size: 723 1177 2333 360
Fragments arranged by size:
2333 1177 723 360
AleI CACnn'nnGTG
Cuts at: 0 3530 4486 4593
Size: 3530 956 107
Fragments arranged by size:
3530 956 107
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 364 1834 4593
Size: 364 1470 2759
Fragments arranged by size:
2759 1470 364
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3211 3243 4593
Size: 3211 32 1350
Fragments arranged by size:
3211 1350 32
AlwNI CAG_nnn'CTG
Cuts at: 0 705 2562 2993 3808 3857 4593
Size: 705 1857 431 815 49 736
Fragments arranged by size:
1857 815 736 705 431 49
ApaI G_GGCC'C
Cuts at: 0 968 1390 4329 4593
Size: 968 422 2939 264
Fragments arranged by size:
2939 968 422 264
ApaLI G'TGCA_C
Cuts at: 0 1686 4593
Size: 1686 2907
ApoI r'AATT_y
Cuts at: 0 1520 3880 4593
Size: 1520 2360 713
Fragments arranged by size:
2360 1520 713
AvaI C'yCGr_G
Cuts at: 0 2336 2570 3427 4322 4593
Size: 2336 234 857 895 271
Fragments arranged by size:
2336 895 857 271 234
BamHI G'GATC_C
Cuts at: 0 840 4593
Size: 840 3753
BanI G'GyrC_C
Cuts at: 0 120 1428 1645 2578 2777 3307 3819 4525
Size: 120 1308 217 933 199 530 512 706
Cuts at: 4525 4593
Size: 68
Fragments arranged by size:
1308 933 706 530 512 217 199 120
68
BanII G_rGCy'C
Cuts at: 0 968 1390 2119 2551 3063 3289 4329 4593
Size: 968 422 729 432 512 226 1040 264
Fragments arranged by size:
1040 968 729 512 432 422 264 226
BbeI G_GCGC'C
Cuts at: 0 2582 3823 4529 4593
Size: 2582 1241 706 64
Fragments arranged by size:
2582 1241 706 64
BbsI GAAGACnn'nnnn_
Cuts at: 0 392 1462 4345 4593
Size: 392 1070 2883 248
Fragments arranged by size:
2883 1070 392 248
BbvCI CC'TCA_GC
Cuts at: 0 2810 2929 4333 4593
Size: 2810 119 1404 260
Fragments arranged by size:
2810 1404 260 119
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3609 3643 4053 4087 4593
Size: 3609 34 410 34 506
Fragments arranged by size:
3609 506 410 34 34
BclI T'GATC_A
Cuts at: 0 1226 4593
Size: 1226 3367
BglI GCCn_nnn'nGGC
Cuts at: 0 2039 2074 4593
Size: 2039 35 2519
Fragments arranged by size:
2519 2039 35
BglII A'GATC_T
Cuts at: 0 1031 2534 4593
Size: 1031 1503 2059
Fragments arranged by size:
2059 1503 1031
BlpI GC'TnA_GC
Cuts at: 0 2740 2986 3046 4593
Size: 2740 246 60 1547
Fragments arranged by size:
2740 1547 246 60
Bme1580I G_kGCm'C
Cuts at: 0 447 968 1390 1433 1648 1690 1921 2102
Size: 447 521 422 43 215 42 231 181
Cuts at: 2102 2235 4329 4593
Size: 133 2094 264
Fragments arranged by size:
2094 521 447 422 264 231 215 181
133 43 42
BmgBI CAC'GTC
Cuts at: 0 746 4593
Size: 746 3847
BmrI ACTGGGnnnn_n'
Cuts at: 0 304 2255 2898 3197 4593
Size: 304 1951 643 299 1396
Fragments arranged by size:
1951 1396 643 304 299
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1988 2565 4593
Size: 1988 577 2028
Fragments arranged by size:
2028 1988 577
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 727 1424 2215 3628 3645 4228 4593
Size: 397 330 697 791 1413 17 583 365
Fragments arranged by size:
1413 791 697 583 397 365 330 17
Bpu10I CC'TnA_GC
Cuts at: 0 2810 2929 4333 4593
Size: 2810 119 1404 260
Fragments arranged by size:
2810 1404 260 119
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3775 4593
Size: 3775 818
BsaI GGTCTCn'nnnn_
Cuts at: 0 2821 3463 4593
Size: 2821 642 1130
Fragments arranged by size:
2821 1130 642
BsaAI yAC'GTr
Cuts at: 0 62 1316 1704 2103 3147 3399 3725 4593
Size: 62 1254 388 399 1044 252 326 868
Fragments arranged by size:
1254 1044 868 399 388 326 252 62
BsaBI GATnn'nnATC
Cuts at: 0 4054 4111 4593
Size: 4054 57 482
Fragments arranged by size:
4054 482 57
BsaHI Gr'CG_yC
Cuts at: 0 2507 2579 2654 3820 4201 4526 4580 4593
Size: 2507 72 75 1166 381 325 54 13
Fragments arranged by size:
2507 1166 381 325 75 72 54 13
BsaWI w'CCGG_w
Cuts at: 0 1775 3884 4415 4593
Size: 1775 2109 531 178
Fragments arranged by size:
2109 1775 531 178
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 730 760 1397 1427 3211 3241 3950 3980
Size: 730 30 637 30 1784 30 709 30
Cuts at: 3980 4593
Size: 613
Fragments arranged by size:
1784 730 709 637 613 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 396 822 898 1615 1882 2227 2650 2739
Size: 396 426 76 717 267 345 423 89
Cuts at: 2739 2742 2839 3520 4593
Size: 3 97 681 1073
Fragments arranged by size:
1073 717 681 426 423 396 345 267
97 89 76 3
BseYI C'CCAG_C
Cuts at: 0 254 848 3477 3571 3850 3991 4593
Size: 254 594 2629 94 279 141 602
Fragments arranged by size:
2629 602 594 279 254 141 94
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1126 1647 1840 2735 3127 3142 4072 4289
Size: 1126 521 193 895 392 15 930 217
Cuts at: 4289 4419 4593
Size: 130 174
Fragments arranged by size:
1126 930 895 521 392 217 193 174
130 15
BsiEI CG_ry'CG
Cuts at: 0 1259 4593
Size: 1259 3334
BsiHKAI G_wGCw'C
Cuts at: 0 1690 3032 4593
Size: 1690 1342 1561
Fragments arranged by size:
1690 1561 1342
BsmI GAATG_Cn'
Cuts at: 0 796 1567 1947 4593
Size: 796 771 380 2646
Fragments arranged by size:
2646 796 771 380
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2567 4593
Size: 2567 2026
Bsp1286I G_dGCh'C
Cuts at: 0 447 968 1390 1433 1648 1690 1921 2102
Size: 447 521 422 43 215 42 231 181
Cuts at: 2102 2119 2235 2551 3032 3063 3289 4329 4593
Size: 17 116 316 481 31 226 1040 264
Fragments arranged by size:
1040 521 481 447 422 316 264 231
226 215 181 116 43 42 31 17
BspEI T'CCGG_A
Cuts at: 0 3884 4415 4593
Size: 3884 531 178
Fragments arranged by size:
3884 531 178
BspHI T'CATG_A
Cuts at: 0 977 2519 4593
Size: 977 1542 2074
Fragments arranged by size:
2074 1542 977
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2490 2859 3161 3705 4593
Size: 2490 369 302 544 888
Fragments arranged by size:
2490 888 544 369 302
BsrBI CCG'CTC
Cuts at: 0 42 3195 3443 4593
Size: 42 3153 248 1150
Fragments arranged by size:
3153 1150 248 42
BsrDI GCAATG_nn'
Cuts at: 0 3709 4593
Size: 3709 884
BsrFI r'CCGG_y
Cuts at: 0 813 4457 4582 4593
Size: 813 3644 125 11
Fragments arranged by size:
3644 813 125 11
BssSI C'ACGA_G
Cuts at: 0 112 1135 4593
Size: 112 1023 3458
Fragments arranged by size:
3458 1023 112
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2395 4593
Size: 2395 2198
BstEII G'GTnAC_C
Cuts at: 0 3173 3717 4593
Size: 3173 544 876
Fragments arranged by size:
3173 876 544
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3237 3579 3774 4593
Size: 3237 342 195 819
Fragments arranged by size:
3237 819 342 195
BstYI r'GATC_y
Cuts at: 0 460 840 1031 1850 2465 2534 3122 4346
Size: 460 380 191 819 615 69 588 1224
Cuts at: 4346 4593
Size: 247
Fragments arranged by size:
1224 819 615 588 460 380 247 191
69
Bsu36I CC'TnA_GG
Cuts at: 0 1846 4593
Size: 1846 2747
BtgI C'CryG_G
Cuts at: 0 1253 1640 1799 2024 2120 3101 3779 4367
Size: 1253 387 159 225 96 981 678 588
Cuts at: 4367 4376 4593
Size: 9 217
Fragments arranged by size:
1253 981 678 588 387 225 217 159
96 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1465 3324 4056 4434 4593
Size: 1465 1859 732 378 159
Fragments arranged by size:
1859 1465 732 378 159
BtsI GCAGTG_nn'
Cuts at: 0 720 1115 2396 4593
Size: 720 395 1281 2197
Fragments arranged by size:
2197 1281 720 395
ClaI AT'CG_AT
Cuts at: 0 4058 4593
Size: 4058 535
DraIII CAC_nnn'GTG
Cuts at: 0 92 4593
Size: 92 4501
DrdI GACnn_nn'nnGTC
Cuts at: 0 55 3010 4438 4593
Size: 55 2955 1428 155
Fragments arranged by size:
2955 1428 155 55
EaeI y'GGCC_r
Cuts at: 0 1310 1586 2021 3104 3329 3581 3596 3776
Size: 1310 276 435 1083 225 252 15 180
Cuts at: 3776 4571 4593
Size: 795 22
Fragments arranged by size:
1310 1083 795 435 276 252 225 180
22 15
EarI CTCTTCn'nnn_
Cuts at: 0 271 1480 2374 2920 4593
Size: 271 1209 894 546 1673
Fragments arranged by size:
1673 1209 894 546 271
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3836 3996 4593
Size: 3836 160 597
Fragments arranged by size:
3836 597 160
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 386 397 727 1424 1627 1636 2215 2470
Size: 386 11 330 697 203 9 579 255
Cuts at: 2470 3439 3559 3628 3645 4228 4243 4593
Size: 969 120 69 17 583 15 350
Fragments arranged by size:
969 697 583 579 386 350 330 255
203 120 69 17 15 11 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1959 4593
Size: 1959 2634
EcoO109I rG'GnC_Cy
Cuts at: 0 965 1071 1218 1387 3912 4125 4326 4593
Size: 965 106 147 169 2525 213 201 267
Fragments arranged by size:
2525 965 267 213 201 169 147 106
EcoRI G'AATT_C
Cuts at: 0 3880 4593
Size: 3880 713
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1372 4593
Size: 1372 3221
HaeII r_GCGC'y
Cuts at: 0 7 26 638 2582 3426 3519 3823 4305
Size: 7 19 612 1944 844 93 304 482
Cuts at: 4305 4529 4593
Size: 224 64
Fragments arranged by size:
1944 844 612 482 304 224 93 64
19 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 189 221 709 730 741 762 1886 1918
Size: 189 32 488 21 11 21 1124 32
Cuts at: 1918 2159 2191 2368 2400 2903 2935 3950 3982
Size: 241 32 177 32 503 32 1015 32
Cuts at: 3982 4037 4069 4219 4251 4593
Size: 55 32 150 32 342
Fragments arranged by size:
1124 1015 503 488 342 241 189 177
150 55 32 32 32 32 32 32
32 32 21 21 11
HincII GTy'rAC
Cuts at: 0 1272 3015 4593
Size: 1272 1743 1578
Fragments arranged by size:
1743 1578 1272
HindIII A'AGCT_T
Cuts at: 0 1546 2875 4593
Size: 1546 1329 1718
Fragments arranged by size:
1718 1546 1329
KasI G'GCGC_C
Cuts at: 0 2578 3819 4525 4593
Size: 2578 1241 706 68
Fragments arranged by size:
2578 1241 706 68
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 728 2375 2999 4337 4593
Size: 59 669 1647 624 1338 256
Fragments arranged by size:
1647 1338 669 624 256 59
MscI TGG'CCA
Cuts at: 0 1312 1588 3331 3583 4573 4593
Size: 1312 276 1743 252 990 20
Fragments arranged by size:
1743 1312 990 276 252 20
MslI CAynn'nnrTG
Cuts at: 0 196 346 1282 1324 1933 2518 2701 3106
Size: 196 150 936 42 609 585 183 405
Cuts at: 3106 3274 3530 3938 4265 4471 4486 4593
Size: 168 256 408 327 206 15 107
Fragments arranged by size:
936 609 585 408 405 327 256 206
196 183 168 150 107 42 15
MspA1I CmG'CkG
Cuts at: 0 375 1192 2193 2660 3137 3417 3857 4593
Size: 375 817 1001 467 477 280 440 736
Fragments arranged by size:
1001 817 736 477 467 440 375 280
NaeI GCC'GGC
Cuts at: 0 4584 4593
Size: 4584 9
NarI GG'CG_CC
Cuts at: 0 2579 3820 4526 4593
Size: 2579 1241 706 67
Fragments arranged by size:
2579 1241 706 67
NcoI C'CATG_G
Cuts at: 0 1799 3101 4593
Size: 1799 1302 1492
Fragments arranged by size:
1799 1492 1302
NgoMIV G'CCGG_C
Cuts at: 0 4582 4593
Size: 4582 11
NspI r_CATG'y
Cuts at: 0 2700 4593
Size: 2700 1893
PasI CC'CwG_GG
Cuts at: 0 3063 4011 4593
Size: 3063 948 582
Fragments arranged by size:
3063 948 582
PflMI CCAn_nnn'nTGG
Cuts at: 0 705 2981 3590 3857 4593
Size: 705 2276 609 267 736
Fragments arranged by size:
2276 736 705 609 267
PfoI T'CCnGG_A
Cuts at: 0 1727 3260 3833 4514 4593
Size: 1727 1533 573 681 79
Fragments arranged by size:
1727 1533 681 573 79
PmlI CAC'GTG
Cuts at: 0 62 2103 3147 4593
Size: 62 2041 1044 1446
Fragments arranged by size:
2041 1446 1044 62
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3211 3243 4593
Size: 3211 32 1350
Fragments arranged by size:
3211 1350 32
PpuMI rG'GwC_Cy
Cuts at: 0 3912 4125 4593
Size: 3912 213 468
Fragments arranged by size:
3912 468 213
PshAI GACnn'nnGTC
Cuts at: 0 1192 4593
Size: 1192 3401
PspOMI G'GGCC_C
Cuts at: 0 964 1386 4325 4593
Size: 964 422 2939 268
Fragments arranged by size:
2939 964 422 268
PspXI vC'TCGA_Gb
Cuts at: 0 3427 4593
Size: 3427 1166
PstI C_TGCA'G
Cuts at: 0 22 1130 2732 2804 4547 4593
Size: 22 1108 1602 72 1743 46
Fragments arranged by size:
1743 1602 1108 72 46 22
PvuII CAG'CTG
Cuts at: 0 375 2193 3417 3857 4593
Size: 375 1818 1224 440 736
Fragments arranged by size:
1818 1224 736 440 375
SapI GCTCTTCn'nnn_
Cuts at: 0 1480 4593
Size: 1480 3113
ScaI AGT'ACT
Cuts at: 0 1150 4180 4593
Size: 1150 3030 413
Fragments arranged by size:
3030 1150 413
SexAI A'CCwGG_T
Cuts at: 0 3212 4593
Size: 3212 1381
SfcI C'TryA_G
Cuts at: 0 18 1126 2728 2757 2800 4543 4563 4593
Size: 18 1108 1602 29 43 1743 20 30
Fragments arranged by size:
1743 1602 1108 43 30 29 20 18
SfoI GGC'GCC
Cuts at: 0 2580 3821 4527 4593
Size: 2580 1241 706 66
Fragments arranged by size:
2580 1241 706 66
SmaI CCC'GGG
Cuts at: 0 2338 4324 4593
Size: 2338 1986 269
Fragments arranged by size:
2338 1986 269
SmlI C'TyrA_G
Cuts at: 0 2570 3427 3790 4593
Size: 2570 857 363 803
Fragments arranged by size:
2570 857 803 363
SphI G_CATG'C
Cuts at: 0 2700 4593
Size: 2700 1893
SrfI GCCC'GGGC
Cuts at: 0 2338 4324 4593
Size: 2338 1986 269
Fragments arranged by size:
2338 1986 269
StuI AGG'CCT
Cuts at: 0 2143 2227 3562 4593
Size: 2143 84 1335 1031
Fragments arranged by size:
2143 1335 1031 84
StyI C'CwwG_G
Cuts at: 0 227 836 866 1799 2438 3101 3230 3363
Size: 227 609 30 933 639 663 129 133
Cuts at: 3363 4593
Size: 1230
Fragments arranged by size:
1230 933 663 639 609 227 133 129
30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 108 197 3192 3423 4593
Size: 108 89 2995 231 1170
Fragments arranged by size:
2995 1170 231 108 89
TatI w'GTAC_w
Cuts at: 0 827 1148 2345 2885 4178 4593
Size: 827 321 1197 540 1293 415
Fragments arranged by size:
1293 1197 827 540 415 321
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1397 1429 3189 3221 4593
Size: 1397 32 1760 32 1372
Fragments arranged by size:
1760 1397 1372 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 225 920 2141 3210 3591 4374 4593
Size: 225 695 1221 1069 381 783 219
Fragments arranged by size:
1221 1069 783 695 381 225 219
XhoI C'TCGA_G
Cuts at: 0 2570 3427 4593
Size: 2570 857 1166
Fragments arranged by size:
2570 1166 857
XmaI C'CCGG_G
Cuts at: 0 2336 4322 4593
Size: 2336 1986 271
Fragments arranged by size:
2336 1986 271
XmnI GAAnn'nnTTC
Cuts at: 0 3223 4228 4593
Size: 3223 1005 365
Fragments arranged by size:
3223 1005 365
ZraI GAC'GTC
Cuts at: 0 2508 4202 4593
Size: 2508 1694 391
Fragments arranged by size:
2508 1694 391
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraIII
DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI HaeII Hin4I HincII HindIII KasI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI PspXI
PstI PvuII SapI ScaI SexAI SfcI SfoI SmaI
SmlI SphI SrfI StuI StyI TaqII TatI TstI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AscI AseI AsiSI AvrII
BaeI BciVI BfrBI BmtI BsiWI BsrGI BssHII BstBI
BstZ17I CspCI DraI EagI EcoICRI EcoRV FseI FspI
FspAI HpaI KpnI MfeI MluI NdeI NheI NotI
NruI NsiI PacI PciI PmeI PsiI PsrI PvuI
RsrII SacI SacII SalI SanDI SbfI SfiI SgfI
SgrAI SnaBI SpeI SspI SwaI Tth111I XbaI