Restriction map for
pF1KB3810
(Linear) (Six-base) MAPSORT of: KIBB3810.seq Check: 1052 from: 1 to: 1500
KIBB3810.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 340 742 1372 1500
Size: 41 299 402 630 128
Fragments arranged by size:
630 402 299 128 41
AleI CACnn'nnGTG
Cuts at: 0 487 1500
Size: 487 1013
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 505 1500
Size: 505 995
ApaLI G'TGCA_C
Cuts at: 0 616 653 1500
Size: 616 37 847
Fragments arranged by size:
847 616 37
AvaI C'yCGr_G
Cuts at: 0 900 1500
Size: 900 600
BamHI G'GATC_C
Cuts at: 0 1152 1392 1500
Size: 1152 240 108
Fragments arranged by size:
1152 240 108
BanII G_rGCy'C
Cuts at: 0 797 901 1500
Size: 797 104 599
Fragments arranged by size:
797 599 104
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 506 540 1500
Size: 506 34 960
Fragments arranged by size:
960 506 34
BclI T'GATC_A
Cuts at: 0 920 1500
Size: 920 580
Bme1580I G_kGCm'C
Cuts at: 0 620 657 1103 1500
Size: 620 37 446 397
Fragments arranged by size:
620 446 397 37
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 1500
Size: 517 983
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1106 1500
Size: 1106 394
BsaI GGTCTCn'nnnn_
Cuts at: 0 1181 1424 1500
Size: 1181 243 76
Fragments arranged by size:
1181 243 76
BsaWI w'CCGG_w
Cuts at: 0 305 599 801 1500
Size: 305 294 202 699
Fragments arranged by size:
699 305 294 202
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 243 273 1500
Size: 243 30 1227
Fragments arranged by size:
1227 243 30
BseYI C'CCAG_C
Cuts at: 0 1361 1500
Size: 1361 139
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 189 1202 1408 1500
Size: 189 1013 206 92
Fragments arranged by size:
1013 206 189 92
BsiHKAI G_wGCw'C
Cuts at: 0 503 620 657 797 901 1500
Size: 503 117 37 140 104 599
Fragments arranged by size:
599 503 140 117 104 37
BsmBI CGTCTCn'nnnn_
Cuts at: 0 349 1500
Size: 349 1151
Bsp1286I G_dGCh'C
Cuts at: 0 503 620 657 797 901 1103 1500
Size: 503 117 37 140 104 202 397
Fragments arranged by size:
503 397 202 140 117 104 37
BspEI T'CCGG_A
Cuts at: 0 599 1500
Size: 599 901
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 772 1500
Size: 772 728
BsrDI GCAATG_nn'
Cuts at: 0 774 1500
Size: 774 726
BsrFI r'CCGG_y
Cuts at: 0 575 1500
Size: 575 925
BstEII G'GTnAC_C
Cuts at: 0 442 1500
Size: 442 1058
BstYI r'GATC_y
Cuts at: 0 904 1152 1383 1392 1500
Size: 904 248 231 9 108
Fragments arranged by size:
904 248 231 108 9
DraI TTT'AAA
Cuts at: 0 408 1280 1500
Size: 408 872 220
Fragments arranged by size:
872 408 220
DraIII CAC_nnn'GTG
Cuts at: 0 612 1500
Size: 612 888
EaeI y'GGCC_r
Cuts at: 0 2 1500
Size: 2 1498
EarI CTCTTCn'nnn_
Cuts at: 0 694 1110 1500
Size: 694 416 390
Fragments arranged by size:
694 416 390
EcoICRI GAG'CTC
Cuts at: 0 795 899 1500
Size: 795 104 601
Fragments arranged by size:
795 601 104
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 340 517 742 1372 1500
Size: 41 299 177 225 630 128
Fragments arranged by size:
630 299 225 177 128 41
EcoO109I rG'GnC_Cy
Cuts at: 0 179 1166 1500
Size: 179 987 334
Fragments arranged by size:
987 334 179
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 693 725 817 849 1500
Size: 693 32 92 32 651
Fragments arranged by size:
693 651 92 32 32
HindIII A'AGCT_T
Cuts at: 0 397 1490 1500
Size: 397 1093 10
Fragments arranged by size:
1093 397 10
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1130 1500
Size: 1130 370
MscI TGG'CCA
Cuts at: 0 4 1500
Size: 4 1496
MslI CAynn'nnrTG
Cuts at: 0 487 613 1500
Size: 487 126 887
Fragments arranged by size:
887 487 126
NdeI CA'TA_TG
Cuts at: 0 692 1020 1500
Size: 692 328 480
Fragments arranged by size:
692 480 328
NspI r_CATG'y
Cuts at: 0 430 950 1500
Size: 430 520 550
Fragments arranged by size:
550 520 430
PpuMI rG'GwC_Cy
Cuts at: 0 1166 1500
Size: 1166 334
SacI G_AGCT'C
Cuts at: 0 797 901 1500
Size: 797 104 599
Fragments arranged by size:
797 599 104
SanDI GG'GwC_CC
Cuts at: 0 1166 1500
Size: 1166 334
SfcI C'TryA_G
Cuts at: 0 1406 1500
Size: 1406 94
SmlI C'TyrA_G
Cuts at: 0 1121 1500
Size: 1121 379
SphI G_CATG'C
Cuts at: 0 430 1500
Size: 430 1070
StuI AGG'CCT
Cuts at: 0 124 214 1500
Size: 124 90 1286
Fragments arranged by size:
1286 124 90
StyI C'CwwG_G
Cuts at: 0 209 1317 1500
Size: 209 1108 183
Fragments arranged by size:
1108 209 183
TatI w'GTAC_w
Cuts at: 0 227 1500
Size: 227 1273
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 29 957 989 1500
Size: 29 928 32 511
Fragments arranged by size:
928 511 32 29
Enzymes that do cut:
AcuI AleI AlfI ApaLI AvaI BamHI BanII BcgI
BclI Bme1580I BpmI BpuEI BsaI BsaWI BsaXI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrDI BsrFI
BstEII BstYI DraI DraIII EaeI EarI EcoICRI Eco57MI
EcoO109I Hin4I HindIII MmeI MscI MslI NdeI NspI
PpuMI SacI SanDI SfcI SmlI SphI StuI StyI
TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AloI AlwNI ApaI ApoI AscI AseI
AsiSI AvrII BaeI BanI BbeI BbsI BbvCI BciVI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI
Bpu10I BsaAI BsaBI BsaHI BseRI BsiEI BsiWI BsmI
BspHI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstXI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DrdI
EagI EciI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HincII HpaI KasI KpnI MfeI MluI
MspA1I NaeI NarI NcoI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacII SalI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI SwaI TaqII Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI