Restriction map for
pF1KB3815
(Linear) (Six-base) MAPSORT of: KIBB3815.seq Check: 6019 from: 1 to: 1209
KIBB3815.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1116 1209
Size: 1116 93
AccI GT'mk_AC
Cuts at: 0 202 940 1209
Size: 202 738 269
Fragments arranged by size:
738 269 202
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 158 1209
Size: 158 1051
AlwNI CAG_nnn'CTG
Cuts at: 0 211 1209
Size: 211 998
ApoI r'AATT_y
Cuts at: 0 1195 1209
Size: 1195 14
AvrII C'CTAG_G
Cuts at: 0 561 1209
Size: 561 648
BamHI G'GATC_C
Cuts at: 0 705 1209
Size: 705 504
BanI G'GyrC_C
Cuts at: 0 361 394 462 693 773 1209
Size: 361 33 68 231 80 436
Fragments arranged by size:
436 361 231 80 68 33
BanII G_rGCy'C
Cuts at: 0 41 169 1209
Size: 41 128 1040
Fragments arranged by size:
1040 128 41
BbeI G_GCGC'C
Cuts at: 0 777 1209
Size: 777 432
BbsI GAAGACnn'nnnn_
Cuts at: 0 207 1209
Size: 207 1002
BbvCI CC'TCA_GC
Cuts at: 0 632 1209
Size: 632 577
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 78 112 1209
Size: 78 34 1097
Fragments arranged by size:
1097 78 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1037 1156 1209
Size: 1037 119 53
Fragments arranged by size:
1037 119 53
BglII A'GATC_T
Cuts at: 0 494 1209
Size: 494 715
BlpI GC'TnA_GC
Cuts at: 0 40 1184 1209
Size: 40 1144 25
Fragments arranged by size:
1144 40 25
Bme1580I G_kGCm'C
Cuts at: 0 147 399 819 1042 1209
Size: 147 252 420 223 167
Fragments arranged by size:
420 252 223 167 147
BmrI ACTGGGnnnn_n'
Cuts at: 0 897 1209
Size: 897 312
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 276 284 532 631 1209
Size: 43 233 8 248 99 578
Fragments arranged by size:
578 248 233 99 43 8
Bpu10I CC'TnA_GC
Cuts at: 0 632 1209
Size: 632 577
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 642 1063 1209
Size: 642 421 146
Fragments arranged by size:
642 421 146
BsaI GGTCTCn'nnnn_
Cuts at: 0 891 1209
Size: 891 318
BsaBI GATnn'nnATC
Cuts at: 0 154 1209
Size: 154 1055
BsaHI Gr'CG_yC
Cuts at: 0 774 955 1209
Size: 774 181 254
Fragments arranged by size:
774 254 181
BsaWI w'CCGG_w
Cuts at: 0 882 1209
Size: 882 327
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 864 894 1209
Size: 864 30 315
Fragments arranged by size:
864 315 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 40 43 1209
Size: 40 3 1166
Fragments arranged by size:
1166 40 3
BseYI C'CCAG_C
Cuts at: 0 627 685 850 958 1035 1209
Size: 627 58 165 108 77 174
Fragments arranged by size:
627 174 165 108 77 58
BsiHKAI G_wGCw'C
Cuts at: 0 41 1209
Size: 41 1168
BsmBI CGTCTCn'nnnn_
Cuts at: 0 922 1209
Size: 922 287
Bsp1286I G_dGCh'C
Cuts at: 0 41 147 169 399 819 1042 1209
Size: 41 106 22 230 420 223 167
Fragments arranged by size:
420 230 223 167 106 41 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 428 986 1116 1209
Size: 428 558 130 93
Fragments arranged by size:
558 428 130 93
BsrBI CCG'CTC
Cuts at: 0 5 858 1188 1209
Size: 5 853 330 21
Fragments arranged by size:
853 330 21 5
BstEII G'GTnAC_C
Cuts at: 0 636 1209
Size: 636 573
BstXI CCAn_nnnn'nTGG
Cuts at: 0 897 1209
Size: 897 312
BstYI r'GATC_y
Cuts at: 0 377 494 705 797 1209
Size: 377 117 211 92 412
Fragments arranged by size:
412 377 211 117 92
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 876 1209
Size: 876 333
BtsI GCAGTG_nn'
Cuts at: 0 147 914 1145 1209
Size: 147 767 231 64
Fragments arranged by size:
767 231 147 64
DrdI GACnn_nn'nnGTC
Cuts at: 0 936 1209
Size: 936 273
EaeI y'GGCC_r
Cuts at: 0 932 1209
Size: 932 277
EcoICRI GAG'CTC
Cuts at: 0 39 1209
Size: 39 1170
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 276 284 532 631 1209
Size: 43 233 8 248 99 578
Fragments arranged by size:
578 248 233 99 43 8
EcoO109I rG'GnC_Cy
Cuts at: 0 255 417 1209
Size: 255 162 792
Fragments arranged by size:
792 255 162
HaeII r_GCGC'y
Cuts at: 0 777 851 1209
Size: 777 74 358
Fragments arranged by size:
777 358 74
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 521 553 1209
Size: 521 32 656
Fragments arranged by size:
656 521 32
KasI G'GCGC_C
Cuts at: 0 773 1209
Size: 773 436
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1088 1209
Size: 1088 121
MspA1I CmG'CkG
Cuts at: 0 211 631 1209
Size: 211 420 578
Fragments arranged by size:
578 420 211
NarI GG'CG_CC
Cuts at: 0 774 1209
Size: 774 435
NspI r_CATG'y
Cuts at: 0 865 1209
Size: 865 344
PasI CC'CwG_GG
Cuts at: 0 1117 1209
Size: 1117 92
PfoI T'CCnGG_A
Cuts at: 0 128 509 1209
Size: 128 381 700
Fragments arranged by size:
700 381 128
PpuMI rG'GwC_Cy
Cuts at: 0 255 417 1209
Size: 255 162 792
Fragments arranged by size:
792 255 162
PvuII CAG'CTG
Cuts at: 0 211 631 1209
Size: 211 420 578
Fragments arranged by size:
578 420 211
SacI G_AGCT'C
Cuts at: 0 41 1209
Size: 41 1168
SfcI C'TryA_G
Cuts at: 0 203 1209
Size: 203 1006
SfoI GGC'GCC
Cuts at: 0 775 1209
Size: 775 434
SmlI C'TyrA_G
Cuts at: 0 657 1078 1209
Size: 657 421 131
Fragments arranged by size:
657 421 131
StuI AGG'CCT
Cuts at: 0 297 1209
Size: 297 912
StyI C'CwwG_G
Cuts at: 0 561 1209
Size: 561 648
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 537 699 1209
Size: 537 162 510
Fragments arranged by size:
537 510 162
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 864 896 1209
Size: 864 32 313
Fragments arranged by size:
864 313 32
XbaI T'CTAG_A
Cuts at: 0 924 1209
Size: 924 285
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 610 687 1092 1209
Size: 610 77 405 117
Fragments arranged by size:
610 405 117 77
Enzymes that do cut:
AarI AccI AlfI AlwNI ApoI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BglI BglII BlpI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiHKAI BsmBI Bsp1286I BspMI
BsrBI BstEII BstXI BstYI BtgZI BtsI DrdI EaeI
EcoICRI Eco57MI EcoO109I HaeII Hin4I KasI MmeI MspA1I
NarI NspI PasI PfoI PpuMI PvuII SacI SfcI
SfoI SmlI StuI StyI TaqII TstI XbaI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvaI BaeI BciVI BclI BfrBI BmgBI BmtI BplI
BsaAI BsgI BsiEI BsiWI BsmI BspEI BspHI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I
BtgI ClaI CspCI DraI DraIII EagI EarI EciI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MscI MslI NaeI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I
XhoI XmaI XmnI ZraI