Restriction map for
pF1KB3816
(Linear) (Six-base) MAPSORT of: KIBB3816.seq Check: 5230 from: 1 to: 6036
KIBB3816.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1185 2011 6036
Size: 1185 826 4025
Fragments arranged by size:
4025 1185 826
AatII G_ACGT'C
Cuts at: 0 2327 6036
Size: 2327 3709
AccI GT'mk_AC
Cuts at: 0 1442 3119 5336 6036
Size: 1442 1677 2217 700
Fragments arranged by size:
2217 1677 1442 700
Acc65I G'GTAC_C
Cuts at: 0 173 1040 6036
Size: 173 867 4996
Fragments arranged by size:
4996 867 173
AclI AA'CG_TT
Cuts at: 0 4299 6036
Size: 4299 1737
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 255 305 796 3779 3855 4919 5830 6036
Size: 255 50 491 2983 76 1064 911 206
Fragments arranged by size:
2983 1064 911 491 255 206 76 50
AfeI AGC'GCT
Cuts at: 0 743 6036
Size: 743 5293
AflIII A'CryG_T
Cuts at: 0 1718 1727 4470 5522 6036
Size: 1718 9 2743 1052 514
Fragments arranged by size:
2743 1718 1052 514 9
AhdI GACnn_n'nnGTC
Cuts at: 0 1173 3980 6036
Size: 1173 2807 2056
Fragments arranged by size:
2807 2056 1173
AleI CACnn'nnGTG
Cuts at: 0 1574 4471 6036
Size: 1574 2897 1565
Fragments arranged by size:
2897 1574 1565
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1167 3508 6036
Size: 1167 2341 2528
Fragments arranged by size:
2528 2341 1167
AlwNI CAG_nnn'CTG
Cuts at: 0 939 1018 2950 3495 3776 4446 4965 6036
Size: 939 79 1932 545 281 670 519 1071
Fragments arranged by size:
1932 1071 939 670 545 519 281 79
ApaI G_GGCC'C
Cuts at: 0 4375 5150 5219 6036
Size: 4375 775 69 817
Fragments arranged by size:
4375 817 775 69
ApoI r'AATT_y
Cuts at: 0 245 2170 2354 3356 3740 6036
Size: 245 1925 184 1002 384 2296
Fragments arranged by size:
2296 1925 1002 384 245 184
AseI AT'TA_AT
Cuts at: 0 2603 6036
Size: 2603 3433
AvaI C'yCGr_G
Cuts at: 0 208 745 3250 5984 6036
Size: 208 537 2505 2734 52
Fragments arranged by size:
2734 2505 537 208 52
BamHI G'GATC_C
Cuts at: 0 212 6036
Size: 212 5824
BanI G'GyrC_C
Cuts at: 0 129 173 749 1040 5749 6036
Size: 129 44 576 291 4709 287
Fragments arranged by size:
4709 576 291 287 129 44
BanII G_rGCy'C
Cuts at: 0 374 1957 2654 2912 2924 4375 4424 4778
Size: 374 1583 697 258 12 1451 49 354
Cuts at: 4778 4995 5150 5219 5834 6036
Size: 217 155 69 615 202
Fragments arranged by size:
1583 1451 697 615 374 354 258 217
202 155 69 49 12
BbsI GAAGACnn'nnnn_
Cuts at: 0 59 417 823 1830 2282 4061 4166 6036
Size: 59 358 406 1007 452 1779 105 1870
Fragments arranged by size:
1870 1779 1007 452 406 358 105 59
BbvCI CC'TCA_GC
Cuts at: 0 2138 2819 3600 5915 6036
Size: 2138 681 781 2315 121
Fragments arranged by size:
2315 2138 781 681 121
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 726 760 6036
Size: 726 34 5276
Fragments arranged by size:
5276 726 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 4225 4813 6036
Size: 4225 588 1223
Fragments arranged by size:
4225 1223 588
BclI T'GATC_A
Cuts at: 0 1373 2255 3698 6036
Size: 1373 882 1443 2338
Fragments arranged by size:
2338 1443 1373 882
BfrBI ATG'CAT
Cuts at: 0 85 6036
Size: 85 5951
BglII A'GATC_T
Cuts at: 0 194 3536 6036
Size: 194 3342 2500
Fragments arranged by size:
3342 2500 194
BlpI GC'TnA_GC
Cuts at: 0 4994 5084 6036
Size: 4994 90 952
Fragments arranged by size:
4994 952 90
Bme1580I G_kGCm'C
Cuts at: 0 134 752 3263 4375 5150 5219 6036
Size: 134 618 2511 1112 775 69 817
Fragments arranged by size:
2511 1112 817 775 618 134 69
BmgBI CAC'GTC
Cuts at: 0 222 1379 4172 5525 6036
Size: 222 1157 2793 1353 511
Fragments arranged by size:
2793 1353 1157 511 222
BmrI ACTGGGnnnn_n'
Cuts at: 0 2190 3095 3257 3393 3974 4101 4419 5153
Size: 2190 905 162 136 581 127 318 734
Cuts at: 5153 6036
Size: 883
Fragments arranged by size:
2190 905 883 734 581 318 162 136
127
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4598 6036
Size: 4598 1438
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 1398 2998 3346 3469 4223 5322 5562
Size: 48 1350 1600 348 123 754 1099 240
Cuts at: 5562 5848 6036
Size: 286 188
Fragments arranged by size:
1600 1350 1099 754 348 286 240 188
123 48
Bpu10I CC'TnA_GC
Cuts at: 0 755 905 2138 2707 2819 3600 5764 5769
Size: 755 150 1233 569 112 781 2164 5
Cuts at: 5769 5915 6036
Size: 146 121
Fragments arranged by size:
2164 1233 781 755 569 150 146 121
112 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1166 1327 1980 3622 4762 6036
Size: 1166 161 653 1642 1140 1274
Fragments arranged by size:
1642 1274 1166 1140 653 161
BsaI GGTCTCn'nnnn_
Cuts at: 0 1863 5020 5409 6036
Size: 1863 3157 389 627
Fragments arranged by size:
3157 1863 627 389
BsaAI yAC'GTr
Cuts at: 0 927 1316 1721 1770 6036
Size: 927 389 405 49 4266
Fragments arranged by size:
4266 927 405 389 49
BsaHI Gr'CG_yC
Cuts at: 0 2324 6036
Size: 2324 3712
BsaWI w'CCGG_w
Cuts at: 0 610 2891 6036
Size: 610 2281 3145
Fragments arranged by size:
3145 2281 610
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 46 76 1705 1735 2519 2549 2842 2872
Size: 46 30 1629 30 784 30 293 30
Cuts at: 2872 3506 3536 4704 4734 5604 5633 5634 5663
Size: 634 30 1168 30 870 29 1 29
Cuts at: 5663 6036
Size: 373
Fragments arranged by size:
1629 1168 870 784 634 373 293 46
30 30 30 30 30 30 29 29
1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 205 435 4020 4866 5412 5844 5853 6036
Size: 205 230 3585 846 546 432 9 183
Fragments arranged by size:
3585 846 546 432 230 205 183 9
BseYI C'CCAG_C
Cuts at: 0 657 712 2185 2459 2911 3408 4291 5212
Size: 657 55 1473 274 452 497 883 921
Cuts at: 5212 5934 6036
Size: 722 102
Fragments arranged by size:
1473 921 883 722 657 497 452 274
102 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1213 1434 2409 3211 3839 6036
Size: 1213 221 975 802 628 2197
Fragments arranged by size:
2197 1213 975 802 628 221
BsiEI CG_ry'CG
Cuts at: 0 5176 6036
Size: 5176 860
BsiHKAI G_wGCw'C
Cuts at: 0 1957 2039 2924 4835 6036
Size: 1957 82 885 1911 1201
Fragments arranged by size:
1957 1911 1201 885 82
BsmI GAATG_Cn'
Cuts at: 0 1517 2767 4976 5208 6036
Size: 1517 1250 2209 232 828
Fragments arranged by size:
2209 1517 1250 828 232
BsmBI CGTCTCn'nnnn_
Cuts at: 0 477 610 3509 6036
Size: 477 133 2899 2527
Fragments arranged by size:
2899 2527 477 133
Bsp1286I G_dGCh'C
Cuts at: 0 134 374 752 1957 2039 2654 2912 2924
Size: 134 240 378 1205 82 615 258 12
Cuts at: 2924 3263 4375 4424 4778 4835 4995 5150 5219
Size: 339 1112 49 354 57 160 155 69
Cuts at: 5219 5834 6036
Size: 615 202
Fragments arranged by size:
1205 1112 615 615 378 354 339 258
240 202 160 155 134 82 69 57
49 12
BspHI T'CATG_A
Cuts at: 0 4913 6036
Size: 4913 1123
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1185 1634 2011 4619 5597 6036
Size: 1185 449 377 2608 978 439
Fragments arranged by size:
2608 1185 978 449 439 377
BsrBI CCG'CTC
Cuts at: 0 1180 6036
Size: 1180 4856
BsrDI GCAATG_nn'
Cuts at: 0 420 1053 2247 4076 6036
Size: 420 633 1194 1829 1960
Fragments arranged by size:
1960 1829 1194 633 420
BsrFI r'CCGG_y
Cuts at: 0 1266 1901 3208 6036
Size: 1266 635 1307 2828
Fragments arranged by size:
2828 1307 1266 635
BsrGI T'GTAC_A
Cuts at: 0 555 6036
Size: 555 5481
BssSI C'ACGA_G
Cuts at: 0 1950 5868 6036
Size: 1950 3918 168
Fragments arranged by size:
3918 1950 168
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2556 4965 6036
Size: 2556 2409 1071
Fragments arranged by size:
2556 2409 1071
BstEII G'GTnAC_C
Cuts at: 0 1926 2143 3067 4788 5686 6036
Size: 1926 217 924 1721 898 350
Fragments arranged by size:
1926 1721 924 898 350 217
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2076 2523 3396 5826 5841 6036
Size: 2076 447 873 2430 15 195
Fragments arranged by size:
2430 2076 873 447 195 15
BstYI r'GATC_y
Cuts at: 0 194 212 508 3536 4096 6036
Size: 194 18 296 3028 560 1940
Fragments arranged by size:
3028 1940 560 296 194 18
Bsu36I CC'TnA_GG
Cuts at: 0 2963 4410 5633 6036
Size: 2963 1447 1223 403
Fragments arranged by size:
2963 1447 1223 403
BtgI C'CryG_G
Cuts at: 0 116 182 530 707 2086 2622 2717 3051
Size: 116 66 348 177 1379 536 95 334
Cuts at: 3051 3575 3608 3892 5045 5819 5900 6036
Size: 524 33 284 1153 774 81 136
Fragments arranged by size:
1379 1153 774 536 524 348 334 284
177 136 116 95 81 66 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 682 2377 6036
Size: 682 1695 3659
Fragments arranged by size:
3659 1695 682
BtsI GCAGTG_nn'
Cuts at: 0 1451 3822 4273 5612 5971 6036
Size: 1451 2371 451 1339 359 65
Fragments arranged by size:
2371 1451 1339 451 359 65
ClaI AT'CG_AT
Cuts at: 0 603 6036
Size: 603 5433
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2823 2858 6036
Size: 2823 35 3178
Fragments arranged by size:
3178 2823 35
DraI TTT'AAA
Cuts at: 0 4115 6036
Size: 4115 1921
DraIII CAC_nnn'GTG
Cuts at: 0 5507 6036
Size: 5507 529
DrdI GACnn_nn'nnGTC
Cuts at: 0 1438 6036
Size: 1438 4598
EaeI y'GGCC_r
Cuts at: 0 1899 1903 2220 2393 2831 5173 5441 5680
Size: 1899 4 317 173 438 2342 268 239
Cuts at: 5680 5903 6036
Size: 223 133
Fragments arranged by size:
2342 1899 438 317 268 239 223 173
133 4
EagI C'GGCC_G
Cuts at: 0 5173 6036
Size: 5173 863
EarI CTCTTCn'nnn_
Cuts at: 0 2525 3798 5003 5465 5640 6036
Size: 2525 1273 1205 462 175 396
Fragments arranged by size:
2525 1273 1205 462 396 175
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 205 1775 2950 4017 5847 6036
Size: 205 1570 1175 1067 1830 189
Fragments arranged by size:
1830 1570 1175 1067 205 189
EcoICRI GAG'CTC
Cuts at: 0 1955 2922 6036
Size: 1955 967 3114
Fragments arranged by size:
3114 1955 967
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 255 305 796 1398 2998 3346 3469
Size: 48 207 50 491 602 1600 348 123
Cuts at: 3469 3779 3855 4223 4919 5322 5562 5830 5848
Size: 310 76 368 696 403 240 268 18
Cuts at: 5848 6036
Size: 188
Fragments arranged by size:
1600 696 602 491 403 368 348 310
268 240 207 188 123 76 50 48
18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1483 2296 6036
Size: 1483 813 3740
Fragments arranged by size:
3740 1483 813
EcoO109I rG'GnC_Cy
Cuts at: 0 1507 3217 3568 3933 4541 4577 5147 5447
Size: 1507 1710 351 365 608 36 570 300
Cuts at: 5447 5760 6036
Size: 313 276
Fragments arranged by size:
1710 1507 608 570 365 351 313 300
276 36
EcoRI G'AATT_C
Cuts at: 0 3356 3740 6036
Size: 3356 384 2296
Fragments arranged by size:
3356 2296 384
EcoRV GAT'ATC
Cuts at: 0 2847 6036
Size: 2847 3189
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3363 6036
Size: 3363 2673
FseI GG_CCGG'CC
Cuts at: 0 1905 6036
Size: 1905 4131
FspI TGC'GCA
Cuts at: 0 1162 6036
Size: 1162 4874
HaeII r_GCGC'y
Cuts at: 0 638 745 5817 6036
Size: 638 107 5072 219
Fragments arranged by size:
5072 638 219 107
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 29 46 78 506 538 1159 1191 1572
Size: 29 17 32 428 32 621 32 381
Cuts at: 1572 1604 1956 1988 2529 2561 2971 3003 3506
Size: 32 352 32 541 32 410 32 503
Cuts at: 3506 3538 3744 3776 3966 3998 4554 4586 5026
Size: 32 206 32 190 32 556 32 440
Cuts at: 5026 5058 5091 5123 5512 5544 6036
Size: 32 33 32 389 32 492
Fragments arranged by size:
621 556 541 503 492 440 428 410
389 381 352 206 190 33 32 32
32 32 32 32 32 32 32 32
32 32 32 32 29 17
HincII GTy'rAC
Cuts at: 0 3120 5057 5540 6036
Size: 3120 1937 483 496
Fragments arranged by size:
3120 1937 496 483
HindIII A'AGCT_T
Cuts at: 0 267 4026 6036
Size: 267 3759 2010
Fragments arranged by size:
3759 2010 267
KpnI G_GTAC'C
Cuts at: 0 177 1044 6036
Size: 177 867 4992
Fragments arranged by size:
4992 867 177
MfeI C'AATT_G
Cuts at: 0 2127 2207 6036
Size: 2127 80 3829
Fragments arranged by size:
3829 2127 80
MluI A'CGCG_T
Cuts at: 0 4470 6036
Size: 4470 1566
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 911 1361 6036
Size: 911 450 4675
Fragments arranged by size:
4675 911 450
MscI TGG'CCA
Cuts at: 0 2395 5443 5905 6036
Size: 2395 3048 462 131
Fragments arranged by size:
3048 2395 462 131
MslI CAynn'nnrTG
Cuts at: 0 1149 1574 2875 3490 4471 5839 6036
Size: 1149 425 1301 615 981 1368 197
Fragments arranged by size:
1368 1301 1149 981 615 425 197
MspA1I CmG'CkG
Cuts at: 0 310 587 770 1448 1833 2029 2459 2823
Size: 310 277 183 678 385 196 430 364
Cuts at: 2823 3128 3577 3592 4073 5919 6036
Size: 305 449 15 481 1846 117
Fragments arranged by size:
1846 678 481 449 430 385 364 310
305 277 196 183 117 15
NaeI GCC'GGC
Cuts at: 0 1903 3210 6036
Size: 1903 1307 2826
Fragments arranged by size:
2826 1903 1307
NcoI C'CATG_G
Cuts at: 0 707 2717 3608 3892 6036
Size: 707 2010 891 284 2144
Fragments arranged by size:
2144 2010 891 707 284
NgoMIV G'CCGG_C
Cuts at: 0 1901 3208 6036
Size: 1901 1307 2828
Fragments arranged by size:
2828 1901 1307
NruI TCG'CGA
Cuts at: 0 37 6036
Size: 37 5999
NsiI A_TGCA'T
Cuts at: 0 87 6036
Size: 87 5949
NspI r_CATG'y
Cuts at: 0 789 2266 2880 5783 6036
Size: 789 1477 614 2903 253
Fragments arranged by size:
2903 1477 789 614 253
PasI CC'CwG_GG
Cuts at: 0 1912 4375 4988 5318 6036
Size: 1912 2463 613 330 718
Fragments arranged by size:
2463 1912 718 613 330
PflMI CCAn_nnn'nTGG
Cuts at: 0 1607 1912 2165 3495 4559 5246 5300 6036
Size: 1607 305 253 1330 1064 687 54 736
Fragments arranged by size:
1607 1330 1064 736 687 305 253 54
PfoI T'CCnGG_A
Cuts at: 0 1427 5825 6036
Size: 1427 4398 211
Fragments arranged by size:
4398 1427 211
PmlI CAC'GTG
Cuts at: 0 1316 1721 1770 6036
Size: 1316 405 49 4266
Fragments arranged by size:
4266 1316 405 49
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4411 4443 6036
Size: 4411 32 1593
Fragments arranged by size:
4411 1593 32
PpuMI rG'GwC_Cy
Cuts at: 0 3568 3933 4577 5760 6036
Size: 3568 365 644 1183 276
Fragments arranged by size:
3568 1183 644 365 276
PsiI TTA'TAA
Cuts at: 0 1111 6036
Size: 1111 4925
PspOMI G'GGCC_C
Cuts at: 0 4371 5146 5215 6036
Size: 4371 775 69 821
Fragments arranged by size:
4371 821 775 69
PstI C_TGCA'G
Cuts at: 0 1006 2552 3948 4244 4616 4640 5328 5608
Size: 1006 1546 1396 296 372 24 688 280
Cuts at: 5608 6036
Size: 428
Fragments arranged by size:
1546 1396 1006 688 428 372 296 280
24
PvuII CAG'CTG
Cuts at: 0 310 2823 4073 6036
Size: 310 2513 1250 1963
Fragments arranged by size:
2513 1963 1250 310
SacI G_AGCT'C
Cuts at: 0 1957 2924 6036
Size: 1957 967 3112
Fragments arranged by size:
3112 1957 967
SacII CC_GC'GG
Cuts at: 0 3578 6036
Size: 3578 2458
SalI G'TCGA_C
Cuts at: 0 3118 6036
Size: 3118 2918
SanDI GG'GwC_CC
Cuts at: 0 3933 6036
Size: 3933 2103
SapI GCTCTTCn'nnn_
Cuts at: 0 5465 6036
Size: 5465 571
SbfI CC_TGCA'GG
Cuts at: 0 4616 6036
Size: 4616 1420
ScaI AGT'ACT
Cuts at: 0 4246 6036
Size: 4246 1790
SexAI A'CCwGG_T
Cuts at: 0 4624 6036
Size: 4624 1412
SfcI C'TryA_G
Cuts at: 0 1002 2548 2865 3480 3735 3944 4240 4612
Size: 1002 1546 317 615 255 209 296 372
Cuts at: 4612 4636 5324 5604 6036
Size: 24 688 280 432
Fragments arranged by size:
1546 1002 688 615 432 372 317 296
280 255 209 24
SmaI CCC'GGG
Cuts at: 0 210 5986 6036
Size: 210 5776 50
Fragments arranged by size:
5776 210 50
SmlI C'TyrA_G
Cuts at: 0 1181 1342 1959 3250 3637 4777 6036
Size: 1181 161 617 1291 387 1140 1259
Fragments arranged by size:
1291 1259 1181 1140 617 387 161
SphI G_CATG'C
Cuts at: 0 5783 6036
Size: 5783 253
SspI AAT'ATT
Cuts at: 0 1941 6036
Size: 1941 4095
StuI AGG'CCT
Cuts at: 0 3196 3715 6036
Size: 3196 519 2321
Fragments arranged by size:
3196 2321 519
StyI C'CwwG_G
Cuts at: 0 707 1920 2516 2717 3338 3608 3892 5207
Size: 707 1213 596 201 621 270 284 1315
Cuts at: 5207 6036
Size: 829
Fragments arranged by size:
1315 1213 829 707 621 596 284 270
201
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2489 3400 3768 3993 5331 6036
Size: 2489 911 368 225 1338 705
Fragments arranged by size:
2489 1338 911 705 368 225
TatI w'GTAC_w
Cuts at: 0 555 1715 3077 3470 3662 3767 4125 4244
Size: 555 1160 1362 393 192 105 358 119
Cuts at: 4244 4535 4711 5558 6036
Size: 291 176 847 478
Fragments arranged by size:
1362 1160 847 555 478 393 358 291
192 176 119 105
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 939 971 1061 1093 1705 1737 2519 2551
Size: 939 32 90 32 612 32 782 32
Cuts at: 2551 2842 2874 3036 3068 5604 5636 6036
Size: 291 32 162 32 2536 32 400
Fragments arranged by size:
2536 939 782 612 400 291 162 90
32 32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 3520 3907 4170 6036
Size: 3520 387 263 1866
Fragments arranged by size:
3520 1866 387 263
XbaI T'CTAG_A
Cuts at: 0 511 6036
Size: 511 5525
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 123 767 2520 6036
Size: 123 644 1753 3516
Fragments arranged by size:
3516 1753 644 123
XhoI C'TCGA_G
Cuts at: 0 3250 6036
Size: 3250 2786
XmaI C'CCGG_G
Cuts at: 0 208 5984 6036
Size: 208 5776 52
Fragments arranged by size:
5776 208 52
XmnI GAAnn'nnTTC
Cuts at: 0 43 3166 6036
Size: 43 3123 2870
Fragments arranged by size:
3123 2870 43
ZraI GAC'GTC
Cuts at: 0 2325 6036
Size: 2325 3711
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AhdI AleI AlfI AlwNI ApaI ApoI AseI AvaI
BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
HaeII Hin4I HincII HindIII KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NgoMIV NruI NsiI
NspI PasI PflMI PfoI PmlI PpiI PpuMI PsiI
PspOMI PstI PvuII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SmaI SmlI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AgeI AloI ApaLI AscI AsiSI AvrII BaeI
BbeI BglI BmtI BsaBI BsiWI BspEI BssHII BstBI
BstZ17I FspAI HpaI KasI NarI NdeI NheI NotI
PacI PciI PmeI PshAI PspXI PsrI PvuI RsrII
SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SwaI