Restriction map for
pF1KB3856
(Linear) (Six-base) MAPSORT of: KIBB3856.seq Check: 7753 from: 1 to: 3723
KIBB3856.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 272 3723
Size: 272 3451
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 688 1826 2030 3723
Size: 688 1138 204 1693
Fragments arranged by size:
1693 1138 688 204
AfeI AGC'GCT
Cuts at: 0 1075 2243 3723
Size: 1075 1168 1480
Fragments arranged by size:
1480 1168 1075
AgeI A'CCGG_T
Cuts at: 0 827 3723
Size: 827 2896
AleI CACnn'nnGTG
Cuts at: 0 1289 3723
Size: 1289 2434
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1846 3622 3723
Size: 1846 1776 101
Fragments arranged by size:
1846 1776 101
AlwNI CAG_nnn'CTG
Cuts at: 0 750 792 1179 1942 2124 2208 2283 2883
Size: 750 42 387 763 182 84 75 600
Cuts at: 2883 3723
Size: 840
Fragments arranged by size:
840 763 750 600 387 182 84 75
42
ApaI G_GGCC'C
Cuts at: 0 73 304 411 570 936 948 1684 2309
Size: 73 231 107 159 366 12 736 625
Cuts at: 2309 3723
Size: 1414
Fragments arranged by size:
1414 736 625 366 231 159 107 73
12
ApoI r'AATT_y
Cuts at: 0 1042 2758 3359 3723
Size: 1042 1716 601 364
Fragments arranged by size:
1716 1042 601 364
AvaI C'yCGr_G
Cuts at: 0 91 327 410 551 654 758 929 941
Size: 91 236 83 141 103 104 171 12
Cuts at: 941 1683 2681 3723
Size: 742 998 1042
Fragments arranged by size:
1042 998 742 236 171 141 104 103
91 83 12
BamHI G'GATC_C
Cuts at: 0 2789 3395 3723
Size: 2789 606 328
Fragments arranged by size:
2789 606 328
BanI G'GyrC_C
Cuts at: 0 17 323 649 659 686 1644 1650 2069
Size: 17 306 326 10 27 958 6 419
Cuts at: 2069 2272 2728 2817 3225 3723
Size: 203 456 89 408 498
Fragments arranged by size:
958 498 456 419 408 326 306 203
89 27 17 10 6
BanII G_rGCy'C
Cuts at: 0 73 304 411 417 570 936 948 1013
Size: 73 231 107 6 153 366 12 65
Cuts at: 1013 1127 1266 1345 1684 1955 2309 2962 3088
Size: 114 139 79 339 271 354 653 126
Cuts at: 3088 3723
Size: 635
Fragments arranged by size:
653 635 366 354 339 271 231 153
139 126 114 107 79 73 65 12
6
BbeI G_GCGC'C
Cuts at: 0 21 327 663 2073 3723
Size: 21 306 336 1410 1650
Fragments arranged by size:
1650 1410 336 306 21
BbsI GAAGACnn'nnnn_
Cuts at: 0 110 212 2203 2250 3003 3723
Size: 110 102 1991 47 753 720
Fragments arranged by size:
1991 753 720 110 102 47
BbvCI CC'TCA_GC
Cuts at: 0 401 3723
Size: 401 3322
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 631 665 3723
Size: 631 34 3058
Fragments arranged by size:
3058 631 34
BglI GCCn_nnn'nGGC
Cuts at: 0 321 397 1017 1147 1771 3559 3723
Size: 321 76 620 130 624 1788 164
Fragments arranged by size:
1788 624 620 321 164 130 76
BglII A'GATC_T
Cuts at: 0 2474 2534 3723
Size: 2474 60 1189
Fragments arranged by size:
2474 1189 60
BlpI GC'TnA_GC
Cuts at: 0 740 2565 2884 3723
Size: 740 1825 319 839
Fragments arranged by size:
1825 839 740 319
Bme1580I G_kGCm'C
Cuts at: 0 73 304 411 570 689 936 948 1684
Size: 73 231 107 159 119 247 12 736
Cuts at: 1684 2277 2309 2822 3141 3723
Size: 593 32 513 319 582
Fragments arranged by size:
736 593 582 513 319 247 231 159
119 107 73 32 12
BmgBI CAC'GTC
Cuts at: 0 1056 1392 3723
Size: 1056 336 2331
Fragments arranged by size:
2331 1056 336
BmrI ACTGGGnnnn_n'
Cuts at: 0 743 1008 2888 3723
Size: 743 265 1880 835
Fragments arranged by size:
1880 835 743 265
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1596 3723
Size: 1596 2127
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 365 1141 1448 1453 1540 1969 2230 2380
Size: 365 776 307 5 87 429 261 150
Cuts at: 2380 2449 3723
Size: 69 1274
Fragments arranged by size:
1274 776 429 365 307 261 150 87
69 5
Bpu10I CC'TnA_GC
Cuts at: 0 401 3723
Size: 401 3322
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1885 3723
Size: 1885 1838
BsaI GGTCTCn'nnnn_
Cuts at: 0 170 1417 2506 3723
Size: 170 1247 1089 1217
Fragments arranged by size:
1247 1217 1089 170
BsaAI yAC'GTr
Cuts at: 0 1092 3723
Size: 1092 2631
BsaBI GATnn'nnATC
Cuts at: 0 1216 3723
Size: 1216 2507
BsaHI Gr'CG_yC
Cuts at: 0 18 79 324 660 2070 3723
Size: 18 61 245 336 1410 1653
Fragments arranged by size:
1653 1410 336 245 61 18
BsaWI w'CCGG_w
Cuts at: 0 270 488 827 3473 3723
Size: 270 218 339 2646 250
Fragments arranged by size:
2646 339 270 250 218
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 148 178 1000 1030 1081 1111 3723
Size: 148 30 822 30 51 30 2612
Fragments arranged by size:
2612 822 148 51 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 157 193 379 438 539 604 726 1075
Size: 157 36 186 59 101 65 122 349
Cuts at: 1075 1078 1873 1927 2338 3723
Size: 3 795 54 411 1385
Fragments arranged by size:
1385 795 411 349 186 157 122 101
65 59 54 36 3
BseYI C'CCAG_C
Cuts at: 0 677 1076 1350 1677 1942 2187 2268 2276
Size: 677 399 274 327 265 245 81 8
Cuts at: 2276 2631 2656 2930 3723
Size: 355 25 274 793
Fragments arranged by size:
793 677 399 355 327 274 274 265
245 81 25 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 506 1870 3723
Size: 475 31 1364 1853
Fragments arranged by size:
1853 1364 475 31
BsiEI CG_ry'CG
Cuts at: 0 803 1719 3630 3723
Size: 803 916 1911 93
Fragments arranged by size:
1911 916 803 93
BsiHKAI G_wGCw'C
Cuts at: 0 1127 1157 2549 2720 3353 3611 3723
Size: 1127 30 1392 171 633 258 112
Fragments arranged by size:
1392 1127 633 258 171 112 30
BsmI GAATG_Cn'
Cuts at: 0 879 1542 3723
Size: 879 663 2181
Fragments arranged by size:
2181 879 663
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2460 3723
Size: 2460 1263
Bsp1286I G_dGCh'C
Cuts at: 0 73 304 411 417 570 689 936 948
Size: 73 231 107 6 153 119 247 12
Cuts at: 948 1013 1127 1157 1266 1345 1684 1955 2277
Size: 65 114 30 109 79 339 271 322
Cuts at: 2277 2309 2549 2720 2822 2962 3088 3141 3353
Size: 32 240 171 102 140 126 53 212
Cuts at: 3353 3611 3723
Size: 258 112
Fragments arranged by size:
339 322 271 258 247 240 231 212
171 153 140 126 119 114 112 109
107 102 79 73 65 53 32 30
12 6
BspEI T'CCGG_A
Cuts at: 0 270 3473 3723
Size: 270 3203 250
Fragments arranged by size:
3203 270 250
BspHI T'CATG_A
Cuts at: 0 815 3209 3723
Size: 815 2394 514
Fragments arranged by size:
2394 815 514
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 272 1799 3113 3723
Size: 272 1527 1314 610
Fragments arranged by size:
1527 1314 610 272
BsrBI CCG'CTC
Cuts at: 0 135 1490 1721 1782 3179 3411 3594 3681
Size: 135 1355 231 61 1397 232 183 87
Cuts at: 3681 3723
Size: 42
Fragments arranged by size:
1397 1355 232 231 183 135 87 61
42
BsrFI r'CCGG_y
Cuts at: 0 827 1647 1922 2725 2771 3666 3723
Size: 827 820 275 803 46 895 57
Fragments arranged by size:
895 827 820 803 275 57 46
BsrGI T'GTAC_A
Cuts at: 0 3368 3723
Size: 3368 355
BssSI C'ACGA_G
Cuts at: 0 167 1504 1708 1756 3723
Size: 167 1337 204 48 1967
Fragments arranged by size:
1967 1337 204 167 48
BstAPI GCAn_nnn'nTGC
Cuts at: 0 750 864 1179 2553 3423 3723
Size: 750 114 315 1374 870 300
Fragments arranged by size:
1374 870 750 315 300 114
BstYI r'GATC_y
Cuts at: 0 146 1161 2474 2534 2789 3321 3335 3395
Size: 146 1015 1313 60 255 532 14 60
Cuts at: 3395 3723
Size: 328
Fragments arranged by size:
1313 1015 532 328 255 146 60 60
14
BtgI C'CryG_G
Cuts at: 0 767 797 1143 1362 1547 1592 3122 3723
Size: 767 30 346 219 185 45 1530 601
Fragments arranged by size:
1530 767 601 346 219 185 45 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2724 2808 3723
Size: 2724 84 915
Fragments arranged by size:
2724 915 84
BtsI GCAGTG_nn'
Cuts at: 0 526 626 748 1640 3723
Size: 526 100 122 892 2083
Fragments arranged by size:
2083 892 526 122 100
DraIII CAC_nnn'GTG
Cuts at: 0 864 895 1200 3723
Size: 864 31 305 2523
Fragments arranged by size:
2523 864 305 31
DrdI GACnn_nn'nnGTC
Cuts at: 0 1390 3723
Size: 1390 2333
EaeI y'GGCC_r
Cuts at: 0 617 794 800 897 1716 1799 2126 2723
Size: 617 177 6 97 819 83 327 597
Cuts at: 2723 2784 3119 3344 3723
Size: 61 335 225 379
Fragments arranged by size:
819 617 597 379 335 327 225 177
97 83 61 6
EagI C'GGCC_G
Cuts at: 0 800 1716 3723
Size: 800 916 2007
Fragments arranged by size:
2007 916 800
EarI CTCTTCn'nnn_
Cuts at: 0 814 1043 1672 2950 3723
Size: 814 229 629 1278 773
Fragments arranged by size:
1278 814 773 629 229
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 610 616 2043 3723
Size: 610 6 1427 1680
Fragments arranged by size:
1680 1427 610 6
EcoICRI GAG'CTC
Cuts at: 0 1125 3723
Size: 1125 2598
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 365 688 1141 1448 1453 1540 1826 1969
Size: 365 323 453 307 5 87 286 143
Cuts at: 1969 2030 2230 2380 2449 3723
Size: 61 200 150 69 1274
Fragments arranged by size:
1274 453 365 323 307 286 200 150
143 87 69 61 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 154 3723
Size: 154 3569
EcoO109I rG'GnC_Cy
Cuts at: 0 70 300 407 566 762 945 1133 1788
Size: 70 230 107 159 196 183 188 655
Cuts at: 1788 2205 2305 2306 3723
Size: 417 100 1 1417
Fragments arranged by size:
1417 655 417 230 196 188 183 159
107 100 70 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 127 2979 3723
Size: 127 2852 744
Fragments arranged by size:
2852 744 127
FspI TGC'GCA
Cuts at: 0 2755 3723
Size: 2755 968
HaeII r_GCGC'y
Cuts at: 0 11 21 327 663 1077 1893 1978 2073
Size: 11 10 306 336 414 816 85 95
Cuts at: 2073 2245 3723
Size: 172 1478
Fragments arranged by size:
1478 816 414 336 306 172 95 85
11 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 148 180 492 524 1942 1974 3627 3659
Size: 148 32 312 32 1418 32 1653 32
Cuts at: 3659 3723
Size: 64
Fragments arranged by size:
1653 1418 312 148 64 32 32 32
32
KasI G'GCGC_C
Cuts at: 0 17 323 659 2069 3723
Size: 17 306 336 1410 1654
Fragments arranged by size:
1654 1410 336 306 17
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1607 1619 2201 2597 3723
Size: 1607 12 582 396 1126
Fragments arranged by size:
1607 1126 582 396 12
MscI TGG'CCA
Cuts at: 0 619 796 3121 3723
Size: 619 177 2325 602
Fragments arranged by size:
2325 619 602 177
MslI CAynn'nnrTG
Cuts at: 0 1289 1361 1735 2839 3025 3208 3449 3604
Size: 1289 72 374 1104 186 183 241 155
Cuts at: 3604 3718 3723
Size: 114 5
Fragments arranged by size:
1289 1104 374 241 186 183 155 114
72 5
MspA1I CmG'CkG
Cuts at: 0 1330 1477 2008 3267 3510 3550 3723
Size: 1330 147 531 1259 243 40 173
Fragments arranged by size:
1330 1259 531 243 173 147 40
NaeI GCC'GGC
Cuts at: 0 1924 2727 3723
Size: 1924 803 996
Fragments arranged by size:
1924 996 803
NarI GG'CG_CC
Cuts at: 0 18 324 660 2070 3723
Size: 18 306 336 1410 1653
Fragments arranged by size:
1653 1410 336 306 18
NcoI C'CATG_G
Cuts at: 0 1143 1362 1592 3122 3723
Size: 1143 219 230 1530 601
Fragments arranged by size:
1530 1143 601 230 219
NgoMIV G'CCGG_C
Cuts at: 0 1922 2725 3723
Size: 1922 803 998
Fragments arranged by size:
1922 998 803
NspI r_CATG'y
Cuts at: 0 3075 3723
Size: 3075 648
PasI CC'CwG_GG
Cuts at: 0 73 123 297 1338 2946 3723
Size: 73 50 174 1041 1608 777
Fragments arranged by size:
1608 1041 777 174 73 50
PflMI CCAn_nnn'nTGG
Cuts at: 0 792 1200 1203 1477 2253 3723
Size: 792 408 3 274 776 1470
Fragments arranged by size:
1470 792 776 408 274 3
PfoI T'CCnGG_A
Cuts at: 0 992 1118 1610 1814 2540 3723
Size: 992 126 492 204 726 1183
Fragments arranged by size:
1183 992 726 492 204 126
PmlI CAC'GTG
Cuts at: 0 1092 3723
Size: 1092 2631
PpuMI rG'GwC_Cy
Cuts at: 0 762 1133 1788 2205 3723
Size: 762 371 655 417 1518
Fragments arranged by size:
1518 762 655 417 371
PspOMI G'GGCC_C
Cuts at: 0 69 300 407 566 932 944 1680 2305
Size: 69 231 107 159 366 12 736 625
Cuts at: 2305 3723
Size: 1418
Fragments arranged by size:
1418 736 625 366 231 159 107 69
12
PstI C_TGCA'G
Cuts at: 0 633 1825 1985 3080 3509 3723
Size: 633 1192 160 1095 429 214
Fragments arranged by size:
1192 1095 633 429 214 160
PvuII CAG'CTG
Cuts at: 0 1330 1477 2008 3510 3550 3723
Size: 1330 147 531 1502 40 173
Fragments arranged by size:
1502 1330 531 173 147 40
SacI G_AGCT'C
Cuts at: 0 1127 3723
Size: 1127 2596
SanDI GG'GwC_CC
Cuts at: 0 762 3723
Size: 762 2961
SapI GCTCTTCn'nnn_
Cuts at: 0 2950 3723
Size: 2950 773
SexAI A'CCwGG_T
Cuts at: 0 1196 2366 3723
Size: 1196 1170 1357
Fragments arranged by size:
1357 1196 1170
SfcI C'TryA_G
Cuts at: 0 151 629 1821 1934 1981 2343 3076 3505
Size: 151 478 1192 113 47 362 733 429
Cuts at: 3505 3723
Size: 218
Fragments arranged by size:
1192 733 478 429 362 218 151 113
47
SfoI GGC'GCC
Cuts at: 0 19 325 661 2071 3723
Size: 19 306 336 1410 1652
Fragments arranged by size:
1652 1410 336 306 19
SmaI CCC'GGG
Cuts at: 0 329 412 760 943 2683 3723
Size: 329 83 348 183 1740 1040
Fragments arranged by size:
1740 1040 348 329 183 83
SmlI C'TyrA_G
Cuts at: 0 1900 3723
Size: 1900 1823
SphI G_CATG'C
Cuts at: 0 3075 3723
Size: 3075 648
SrfI GCCC'GGGC
Cuts at: 0 412 3723
Size: 412 3311
StuI AGG'CCT
Cuts at: 0 2329 3723
Size: 2329 1394
StyI C'CwwG_G
Cuts at: 0 26 1143 1362 1592 3122 3723
Size: 26 1117 219 230 1530 601
Fragments arranged by size:
1530 1117 601 230 219 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 263 274 1301 2260 2482 3461 3644 3723
Size: 263 11 1027 959 222 979 183 79
Fragments arranged by size:
1027 979 959 263 222 183 79 11
TatI w'GTAC_w
Cuts at: 0 3368 3698 3723
Size: 3368 330 25
Fragments arranged by size:
3368 330 25
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 211 238 243 270 569 601 1000 1032
Size: 211 27 5 27 299 32 399 32
Cuts at: 1032 1081 1113 2891 2923 3723
Size: 49 32 1778 32 800
Fragments arranged by size:
1778 800 399 299 211 49 32 32
32 32 27 27 5
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2835 3723
Size: 2835 888
XmaI C'CCGG_G
Cuts at: 0 327 410 758 941 2681 3723
Size: 327 83 348 183 1740 1042
Fragments arranged by size:
1740 1042 348 327 183 83
XmnI GAAnn'nnTTC
Cuts at: 0 1303 2536 3723
Size: 1303 1233 1187
Fragments arranged by size:
1303 1233 1187
Enzymes that do cut:
AarI AcuI AfeI AgeI AleI AlfI AlwNI ApaI
ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BsrGI BssSI
BstAPI BstYI BtgI BtgZI BtsI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
FspI HaeII Hin4I KasI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI
PmlI PpuMI PspOMI PstI PvuII SacI SanDI SapI
SexAI SfcI SfoI SmaI SmlI SphI SrfI StuI
StyI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AhdI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BciVI BclI
BfrBI BmtI BsiWI BsrDI BssHII BstBI BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraI EcoRI EcoRV FseI
FspAI HincII HindIII HpaI KpnI MfeI MluI NdeI
NheI NotI NruI NsiI PacI PciI PmeI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SspI
SwaI Tth111I XbaI XhoI ZraI