Restriction map for
pF1KB3865
(Linear) (Six-base) MAPSORT of: KIBB3865.seq Check: 7555 from: 1 to: 1002
KIBB3865.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 437 1002
Size: 437 565
AccI GT'mk_AC
Cuts at: 0 572 1002
Size: 572 430
AflIII A'CryG_T
Cuts at: 0 212 1002
Size: 212 790
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 924 1002
Size: 924 78
BanI G'GyrC_C
Cuts at: 0 71 148 496 1002
Size: 71 77 348 506
Fragments arranged by size:
506 348 77 71
BanII G_rGCy'C
Cuts at: 0 611 896 1002
Size: 611 285 106
Fragments arranged by size:
611 285 106
BbeI G_GCGC'C
Cuts at: 0 152 1002
Size: 152 850
BbsI GAAGACnn'nnnn_
Cuts at: 0 730 1002
Size: 730 272
BglI GCCn_nnn'nGGC
Cuts at: 0 563 1002
Size: 563 439
Bme1580I G_kGCm'C
Cuts at: 0 338 499 1002
Size: 338 161 503
Fragments arranged by size:
503 338 161
BmgBI CAC'GTC
Cuts at: 0 173 1002
Size: 173 829
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 537 625 910 1002
Size: 537 88 285 92
Fragments arranged by size:
537 285 92 88
BsaI GGTCTCn'nnnn_
Cuts at: 0 529 1002
Size: 529 473
BsaAI yAC'GTr
Cuts at: 0 213 261 333 435 1002
Size: 213 48 72 102 567
Fragments arranged by size:
567 213 102 72 48
BsaHI Gr'CG_yC
Cuts at: 0 149 268 379 442 470 1002
Size: 149 119 111 63 28 532
Fragments arranged by size:
532 149 119 111 63 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 310 313 415 894 1002
Size: 153 157 3 102 479 108
Fragments arranged by size:
479 157 153 108 102 3
BsiEI CG_ry'CG
Cuts at: 0 29 1002
Size: 29 973
BsiHKAI G_wGCw'C
Cuts at: 0 82 611 1002
Size: 82 529 391
Fragments arranged by size:
529 391 82
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3 433 461 1002
Size: 3 430 28 541
Fragments arranged by size:
541 430 28 3
Bsp1286I G_dGCh'C
Cuts at: 0 82 338 499 611 896 1002
Size: 82 256 161 112 285 106
Fragments arranged by size:
285 256 161 112 106 82
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 437 791 1002
Size: 437 354 211
Fragments arranged by size:
437 354 211
BsrFI r'CCGG_y
Cuts at: 0 409 743 1002
Size: 409 334 259
Fragments arranged by size:
409 334 259
BsrGI T'GTAC_A
Cuts at: 0 215 1002
Size: 215 787
BssHII G'CGCG_C
Cuts at: 0 391 1002
Size: 391 611
BstBI TT'CG_AA
Cuts at: 0 614 1002
Size: 614 388
BstXI CCAn_nnnn'nTGG
Cuts at: 0 261 921 1002
Size: 261 660 81
Fragments arranged by size:
660 261 81
BstYI r'GATC_y
Cuts at: 0 917 1002
Size: 917 85
BtgI C'CryG_G
Cuts at: 0 232 490 688 1002
Size: 232 258 198 314
Fragments arranged by size:
314 258 232 198
BtsI GCAGTG_nn'
Cuts at: 0 126 1002
Size: 126 876
DraIII CAC_nnn'GTG
Cuts at: 0 264 1002
Size: 264 738
EaeI y'GGCC_r
Cuts at: 0 314 745 1002
Size: 314 431 257
Fragments arranged by size:
431 314 257
EarI CTCTTCn'nnn_
Cuts at: 0 1 611 616 1002
Size: 1 610 5 386
Fragments arranged by size:
610 386 5 1
EcoICRI GAG'CTC
Cuts at: 0 609 1002
Size: 609 393
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 537 625 910 1002
Size: 537 88 285 92
Fragments arranged by size:
537 285 92 88
EcoO109I rG'GnC_Cy
Cuts at: 0 684 1002
Size: 684 318
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 993 1002
Size: 993 9
FspI TGC'GCA
Cuts at: 0 202 625 1002
Size: 202 423 377
Fragments arranged by size:
423 377 202
FspAI rTGC'GCAy
Cuts at: 0 202 1002
Size: 202 800
HaeII r_GCGC'y
Cuts at: 0 152 1002
Size: 152 850
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2 34 761 793 857 889 1002
Size: 2 32 727 32 64 32 113
Fragments arranged by size:
727 113 64 32 32 32 2
HincII GTy'rAC
Cuts at: 0 780 1002
Size: 780 222
KasI G'GCGC_C
Cuts at: 0 148 1002
Size: 148 854
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 263 1002
Size: 263 739
MscI TGG'CCA
Cuts at: 0 316 1002
Size: 316 686
MslI CAynn'nnrTG
Cuts at: 0 110 259 1002
Size: 110 149 743
Fragments arranged by size:
743 149 110
MspA1I CmG'CkG
Cuts at: 0 384 492 1002
Size: 384 108 510
Fragments arranged by size:
510 384 108
NarI GG'CG_CC
Cuts at: 0 149 1002
Size: 149 853
NcoI C'CATG_G
Cuts at: 0 232 1002
Size: 232 770
PasI CC'CwG_GG
Cuts at: 0 158 1002
Size: 158 844
PflMI CCAn_nnn'nTGG
Cuts at: 0 351 1002
Size: 351 651
PmlI CAC'GTG
Cuts at: 0 261 333 435 1002
Size: 261 72 102 567
Fragments arranged by size:
567 261 102 72
PstI C_TGCA'G
Cuts at: 0 800 1002
Size: 800 202
PvuI CG_AT'CG
Cuts at: 0 29 1002
Size: 29 973
SacI G_AGCT'C
Cuts at: 0 611 1002
Size: 611 391
SacII CC_GC'GG
Cuts at: 0 493 1002
Size: 493 509
SapI GCTCTTCn'nnn_
Cuts at: 0 616 1002
Size: 616 386
ScaI AGT'ACT
Cuts at: 0 166 1002
Size: 166 836
SfcI C'TryA_G
Cuts at: 0 276 597 796 1002
Size: 276 321 199 206
Fragments arranged by size:
321 276 206 199
SfoI GGC'GCC
Cuts at: 0 150 1002
Size: 150 852
StuI AGG'CCT
Cuts at: 0 811 1002
Size: 811 191
StyI C'CwwG_G
Cuts at: 0 232 896 1002
Size: 232 664 106
Fragments arranged by size:
664 232 106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 655 1002
Size: 655 347
TatI w'GTAC_w
Cuts at: 0 164 215 1002
Size: 164 51 787
Fragments arranged by size:
787 164 51
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 258 1002
Size: 258 744
XmnI GAAnn'nnTTC
Cuts at: 0 976 1002
Size: 976 26
Enzymes that do cut:
AarI AccI AflIII AlfI BanI BanII BbeI BbsI
BglI Bme1580I BmgBI BpmI BsaI BsaAI BsaHI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BsrGI BssHII
BstBI BstXI BstYI BtgI BtsI DraIII EaeI EarI
EcoICRI Eco57MI EcoO109I FalI FspI FspAI HaeII Hin4I
HincII KasI MmeI MscI MslI MspA1I NarI NcoI
PasI PflMI PmlI PstI PvuI SacI SacII SapI
ScaI SfcI SfoI StuI StyI TaqII TatI XcmI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI
AleI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmrI BmtI BplI Bpu10I
BpuEI BsaBI BsaWI BsaXI BseRI BseYI BsiWI BsmI
BspEI BspHI BsrBI BsrDI BssSI BstAPI BstEII BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EciI
EcoNI EcoRI EcoRV FseI HindIII HpaI KpnI MfeI
MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuII RsrII SalI SanDI
SbfI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI
XhoI XmaI ZraI