Restriction map for
pF1KB3867
(Linear) (Six-base) MAPSORT of: KIBB3867.seq Check: 59 from: 1 to: 1062
KIBB3867.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AatII G_ACGT'C
Cuts at: 0 605 656 1062
Size: 605 51 406
Fragments arranged by size:
605 406 51
AccI GT'mk_AC
Cuts at: 0 218 1062
Size: 218 844
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 573 1062
Size: 573 489
AgeI A'CCGG_T
Cuts at: 0 425 1062
Size: 425 637
AleI CACnn'nnGTG
Cuts at: 0 145 1062
Size: 145 917
AlwNI CAG_nnn'CTG
Cuts at: 0 240 1062
Size: 240 822
AvaI C'yCGr_G
Cuts at: 0 37 115 431 1062
Size: 37 78 316 631
Fragments arranged by size:
631 316 78 37
BamHI G'GATC_C
Cuts at: 0 635 1062
Size: 635 427
BanII G_rGCy'C
Cuts at: 0 356 1062
Size: 356 706
BbsI GAAGACnn'nnnn_
Cuts at: 0 197 350 716 899 1062
Size: 197 153 366 183 163
Fragments arranged by size:
366 197 183 163 153
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 711 745 1062
Size: 711 34 317
Fragments arranged by size:
711 317 34
BglII A'GATC_T
Cuts at: 0 816 1062
Size: 816 246
BlpI GC'TnA_GC
Cuts at: 0 682 1062
Size: 682 380
BmgBI CAC'GTC
Cuts at: 0 99 195 1062
Size: 99 96 867
Fragments arranged by size:
867 99 96
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 52 375 1062
Size: 52 323 687
Fragments arranged by size:
687 323 52
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 1062
Size: 143 919
BsaBI GATnn'nnATC
Cuts at: 0 160 1062
Size: 160 902
BsaHI Gr'CG_yC
Cuts at: 0 308 602 653 1010 1062
Size: 308 294 51 357 52
Fragments arranged by size:
357 308 294 52 51
BsaWI w'CCGG_w
Cuts at: 0 184 425 1062
Size: 184 241 637
Fragments arranged by size:
637 241 184
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 179 209 1062
Size: 179 30 853
Fragments arranged by size:
853 179 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 37 1062
Size: 37 1025
BsiHKAI G_wGCw'C
Cuts at: 0 704 1062
Size: 704 358
Bsp1286I G_dGCh'C
Cuts at: 0 356 704 1062
Size: 356 348 358
Fragments arranged by size:
358 356 348
BspEI T'CCGG_A
Cuts at: 0 184 1062
Size: 184 878
BsrBI CCG'CTC
Cuts at: 0 42 938 1062
Size: 42 896 124
Fragments arranged by size:
896 124 42
BsrDI GCAATG_nn'
Cuts at: 0 639 1028 1062
Size: 639 389 34
Fragments arranged by size:
639 389 34
BsrFI r'CCGG_y
Cuts at: 0 425 1062
Size: 425 637
BssSI C'ACGA_G
Cuts at: 0 733 1062
Size: 733 329
BstXI CCAn_nnnn'nTGG
Cuts at: 0 175 1062
Size: 175 887
BstYI r'GATC_y
Cuts at: 0 635 816 1062
Size: 635 181 246
Fragments arranged by size:
635 246 181
BtgI C'CryG_G
Cuts at: 0 260 1062
Size: 260 802
ClaI AT'CG_AT
Cuts at: 0 782 1062
Size: 782 280
EaeI y'GGCC_r
Cuts at: 0 659 1062
Size: 659 403
EarI CTCTTCn'nnn_
Cuts at: 0 754 805 1062
Size: 754 51 257
Fragments arranged by size:
754 257 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 573 1062
Size: 143 430 489
Fragments arranged by size:
489 430 143
EcoNI CCTnn'n_nnAGG
Cuts at: 0 70 1062
Size: 70 992
EcoO109I rG'GnC_Cy
Cuts at: 0 877 1062
Size: 877 185
HaeII r_GCGC'y
Cuts at: 0 89 1062
Size: 89 973
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 166 198 1062
Size: 166 32 864
Fragments arranged by size:
864 166 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 585 1062
Size: 316 269 477
Fragments arranged by size:
477 316 269
MslI CAynn'nnrTG
Cuts at: 0 145 173 997 1062
Size: 145 28 824 65
Fragments arranged by size:
824 145 65 28
MspA1I CmG'CkG
Cuts at: 0 686 1062
Size: 686 376
NcoI C'CATG_G
Cuts at: 0 260 1062
Size: 260 802
NspI r_CATG'y
Cuts at: 0 732 1062
Size: 732 330
PflMI CCAn_nnn'nTGG
Cuts at: 0 240 1062
Size: 240 822
PspXI vC'TCGA_Gb
Cuts at: 0 37 1062
Size: 37 1025
PstI C_TGCA'G
Cuts at: 0 366 1062
Size: 366 696
SapI GCTCTTCn'nnn_
Cuts at: 0 754 1062
Size: 754 308
SexAI A'CCwGG_T
Cuts at: 0 695 1062
Size: 695 367
SfcI C'TryA_G
Cuts at: 0 219 362 1062
Size: 219 143 700
Fragments arranged by size:
700 219 143
SmaI CCC'GGG
Cuts at: 0 433 1062
Size: 433 629
SmlI C'TyrA_G
Cuts at: 0 37 1062
Size: 37 1025
SphI G_CATG'C
Cuts at: 0 732 1062
Size: 732 330
StyI C'CwwG_G
Cuts at: 0 260 1062
Size: 260 802
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 957 1062
Size: 957 105
TatI w'GTAC_w
Cuts at: 0 7 203 1062
Size: 7 196 859
Fragments arranged by size:
859 196 7
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 179 211 1062
Size: 179 32 851
Fragments arranged by size:
851 179 32
Tth111I GACn'n_nGTC
Cuts at: 0 1012 1062
Size: 1012 50
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 267 465 1062
Size: 267 198 597
Fragments arranged by size:
597 267 198
XhoI C'TCGA_G
Cuts at: 0 37 1062
Size: 37 1025
XmaI C'CCGG_G
Cuts at: 0 431 1062
Size: 431 631
ZraI GAC'GTC
Cuts at: 0 603 654 1062
Size: 603 51 408
Fragments arranged by size:
603 408 51
Enzymes that do cut:
AatII AccI AcuI AgeI AleI AlwNI AvaI BamHI
BanII BbsI BcgI BglII BlpI BmgBI BplI BpmI
BsaBI BsaHI BsaWI BsaXI BseRI BsiHKAI Bsp1286I BspEI
BsrBI BsrDI BsrFI BssSI BstXI BstYI BtgI ClaI
EaeI EarI Eco57MI EcoNI EcoO109I HaeII Hin4I MmeI
MslI MspA1I NcoI NspI PflMI PspXI PstI SapI
SexAI SfcI SmaI SmlI SphI StyI TaqII TatI
TstI Tth111I XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AhdI AlfI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BanI BbeI BbvCI BciVI BclI BfrBI BglI
Bme1580I BmrI BmtI Bpu10I BpuEI BsaI BsaAI BseYI
BsgI BsiEI BsiWI BsmI BsmBI BspHI BspMI BsrGI
BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI
CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MscI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI
SrfI SspI StuI SwaI XbaI XmnI