Restriction map for
pF1KB3889
(Linear) (Six-base) MAPSORT of: KIBB3889.seq Check: 4169 from: 1 to: 2424
KIBB3889.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1938 2424
Size: 1938 486
AatII G_ACGT'C
Cuts at: 0 1099 2424
Size: 1099 1325
Acc65I G'GTAC_C
Cuts at: 0 1994 2424
Size: 1994 430
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 591 655 1315 1641 1720 2424
Size: 591 64 660 326 79 704
Fragments arranged by size:
704 660 591 326 79 64
AfeI AGC'GCT
Cuts at: 0 1426 2267 2424
Size: 1426 841 157
Fragments arranged by size:
1426 841 157
AgeI A'CCGG_T
Cuts at: 0 551 2424
Size: 551 1873
AhdI GACnn_n'nnGTC
Cuts at: 0 251 2424
Size: 251 2173
AleI CACnn'nnGTG
Cuts at: 0 1562 2424
Size: 1562 862
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1747 2424
Size: 1747 677
AlwNI CAG_nnn'CTG
Cuts at: 0 832 1539 1605 1734 2424
Size: 832 707 66 129 690
Fragments arranged by size:
832 707 690 129 66
ApaI G_GGCC'C
Cuts at: 0 871 1034 1521 2424
Size: 871 163 487 903
Fragments arranged by size:
903 871 487 163
ApaLI G'TGCA_C
Cuts at: 0 460 2424
Size: 460 1964
AvaI C'yCGr_G
Cuts at: 0 159 1033 1880 2230 2424
Size: 159 874 847 350 194
Fragments arranged by size:
874 847 350 194 159
BanI G'GyrC_C
Cuts at: 0 130 1273 1627 1994 2424
Size: 130 1143 354 367 430
Fragments arranged by size:
1143 430 367 354 130
BanII G_rGCy'C
Cuts at: 0 871 1034 1521 2424
Size: 871 163 487 903
Fragments arranged by size:
903 871 487 163
BbsI GAAGACnn'nnnn_
Cuts at: 0 1539 2424
Size: 1539 885
BbvCI CC'TCA_GC
Cuts at: 0 220 1617 2424
Size: 220 1397 807
Fragments arranged by size:
1397 807 220
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 678 712 2424
Size: 678 34 1712
Fragments arranged by size:
1712 678 34
BglI GCCn_nnn'nGGC
Cuts at: 0 163 256 334 343 2424
Size: 163 93 78 9 2081
Fragments arranged by size:
2081 163 93 78 9
BglII A'GATC_T
Cuts at: 0 650 2424
Size: 650 1774
BlpI GC'TnA_GC
Cuts at: 0 631 2311 2424
Size: 631 1680 113
Fragments arranged by size:
1680 631 113
Bme1580I G_kGCm'C
Cuts at: 0 464 871 1034 1276 1521 1647 1685 2424
Size: 464 407 163 242 245 126 38 739
Fragments arranged by size:
739 464 407 245 242 163 126 38
BmrI ACTGGGnnnn_n'
Cuts at: 0 1874 2424
Size: 1874 550
BmtI G_CTAG'C
Cuts at: 0 373 2424
Size: 373 2051
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 981 1906 2424
Size: 981 925 518
Fragments arranged by size:
981 925 518
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 1227 1633 2308 2424
Size: 202 1025 406 675 116
Fragments arranged by size:
1025 675 406 202 116
Bpu10I CC'TnA_GC
Cuts at: 0 220 1244 1617 2424
Size: 220 1024 373 807
Fragments arranged by size:
1024 807 373 220
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1651 2235 2290 2424
Size: 1651 584 55 134
Fragments arranged by size:
1651 584 134 55
BsaI GGTCTCn'nnnn_
Cuts at: 0 1 2424
Size: 1 2423
BsaAI yAC'GTr
Cuts at: 0 2159 2424
Size: 2159 265
BsaBI GATnn'nnATC
Cuts at: 0 655 2424
Size: 655 1769
BsaHI Gr'CG_yC
Cuts at: 0 1096 2424
Size: 1096 1328
BsaWI w'CCGG_w
Cuts at: 0 551 1019 1154 1252 2066 2424
Size: 551 468 135 98 814 358
Fragments arranged by size:
814 551 468 358 135 98
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 343 373 733 763 2424
Size: 343 30 360 30 1661
Fragments arranged by size:
1661 360 343 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 498 754 1018 1939 1957 2248 2424
Size: 498 256 264 921 18 291 176
Fragments arranged by size:
921 498 291 264 256 176 18
BseYI C'CCAG_C
Cuts at: 0 752 756 857 1539 2007 2202 2424
Size: 752 4 101 682 468 195 222
Fragments arranged by size:
752 682 468 222 195 101 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 876 1314 1816 2424
Size: 876 438 502 608
Fragments arranged by size:
876 608 502 438
BsiEI CG_ry'CG
Cuts at: 0 17 642 2381 2424
Size: 17 625 1739 43
Fragments arranged by size:
1739 625 43 17
BsiHKAI G_wGCw'C
Cuts at: 0 464 2213 2424
Size: 464 1749 211
Fragments arranged by size:
1749 464 211
Bsp1286I G_dGCh'C
Cuts at: 0 464 871 1034 1276 1521 1647 1685 2213
Size: 464 407 163 242 245 126 38 528
Cuts at: 2213 2424
Size: 211
Fragments arranged by size:
528 464 407 245 242 211 163 126
38
BspEI T'CCGG_A
Cuts at: 0 1154 2066 2424
Size: 1154 912 358
Fragments arranged by size:
1154 912 358
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1938 1985 2006 2424
Size: 1938 47 21 418
Fragments arranged by size:
1938 418 47 21
BsrBI CCG'CTC
Cuts at: 0 2295 2424
Size: 2295 129
BsrDI GCAATG_nn'
Cuts at: 0 259 2424
Size: 259 2165
BsrFI r'CCGG_y
Cuts at: 0 551 910 1559 1841 2376 2424
Size: 551 359 649 282 535 48
Fragments arranged by size:
649 551 535 359 282 48
BsrGI T'GTAC_A
Cuts at: 0 845 2424
Size: 845 1579
BssHII G'CGCG_C
Cuts at: 0 2414 2424
Size: 2414 10
BssSI C'ACGA_G
Cuts at: 0 687 1756 1810 2185 2424
Size: 687 1069 54 375 239
Fragments arranged by size:
1069 687 375 239 54
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1280 2424
Size: 1280 1144
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1564 2025 2424
Size: 1564 461 399
Fragments arranged by size:
1564 461 399
BstYI r'GATC_y
Cuts at: 0 650 1109 2424
Size: 650 459 1315
Fragments arranged by size:
1315 650 459
Bsu36I CC'TnA_GG
Cuts at: 0 2078 2424
Size: 2078 346
BtgI C'CryG_G
Cuts at: 0 11 332 341 515 1116 1592 1610 1719
Size: 11 321 9 174 601 476 18 109
Cuts at: 1719 2424
Size: 705
Fragments arranged by size:
705 601 476 321 174 109 18 11
9
BtsI GCAGTG_nn'
Cuts at: 0 206 541 844 888 1833 2178 2424
Size: 206 335 303 44 945 345 246
Fragments arranged by size:
945 345 335 303 246 206 44
DraIII CAC_nnn'GTG
Cuts at: 0 468 1269 2043 2424
Size: 468 801 774 381
Fragments arranged by size:
801 774 468 381
DrdI GACnn_nn'nnGTC
Cuts at: 0 1882 2424
Size: 1882 542
EaeI y'GGCC_r
Cuts at: 0 14 105 335 512 618 639 1119 1224
Size: 14 91 230 177 106 21 480 105
Cuts at: 1224 1481 1505 1607 2015 2378 2424
Size: 257 24 102 408 363 46
Fragments arranged by size:
480 408 363 257 230 177 106 105
102 91 46 24 21 14
EagI C'GGCC_G
Cuts at: 0 14 639 2378 2424
Size: 14 625 1739 46
Fragments arranged by size:
1739 625 46 14
EarI CTCTTCn'nnn_
Cuts at: 0 121 508 1355 2424
Size: 121 387 847 1069
Fragments arranged by size:
1069 847 387 121
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 95 1927 2424
Size: 95 1832 497
Fragments arranged by size:
1832 497 95
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 591 655 1227 1315 1633 1641 1720
Size: 202 389 64 572 88 318 8 79
Cuts at: 1720 2308 2424
Size: 588 116
Fragments arranged by size:
588 572 389 318 202 116 88 79
64 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 310 1405 1894 2424
Size: 310 1095 489 530
Fragments arranged by size:
1095 530 489 310
EcoO109I rG'GnC_Cy
Cuts at: 0 666 868 1518 2073 2136 2226 2274 2318
Size: 666 202 650 555 63 90 48 44
Cuts at: 2318 2424
Size: 106
Fragments arranged by size:
666 650 555 202 106 90 63 48
44
EcoRV GAT'ATC
Cuts at: 0 1131 2424
Size: 1131 1293
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1320 1725 2424
Size: 1320 405 699
Fragments arranged by size:
1320 699 405
HaeII r_GCGC'y
Cuts at: 0 590 614 1428 2008 2269 2424
Size: 590 24 814 580 261 155
Fragments arranged by size:
814 590 580 261 155 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 237 269 343 375 733 765 2424
Size: 237 32 74 32 358 32 1659
Fragments arranged by size:
1659 358 237 74 32 32 32
HincII GTy'rAC
Cuts at: 0 141 2424
Size: 141 2283
KpnI G_GTAC'C
Cuts at: 0 1998 2424
Size: 1998 426
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2136 2424
Size: 2136 288
MscI TGG'CCA
Cuts at: 0 107 337 1226 1483 1609 2017 2424
Size: 107 230 889 257 126 408 407
Fragments arranged by size:
889 408 407 257 230 126 107
MslI CAynn'nnrTG
Cuts at: 0 532 1562 2424
Size: 532 1030 862
Fragments arranged by size:
1030 862 532
MspA1I CmG'CkG
Cuts at: 0 13 93 756 793 1178 1536 1621 1661
Size: 13 80 663 37 385 358 85 40
Cuts at: 1661 1731 1742 2307 2424
Size: 70 11 565 117
Fragments arranged by size:
663 565 385 358 117 85 80 70
40 37 13 11
NaeI GCC'GGC
Cuts at: 0 912 1843 2378 2424
Size: 912 931 535 46
Fragments arranged by size:
931 912 535 46
NcoI C'CATG_G
Cuts at: 0 341 1592 2424
Size: 341 1251 832
Fragments arranged by size:
1251 832 341
NgoMIV G'CCGG_C
Cuts at: 0 910 1841 2376 2424
Size: 910 931 535 48
Fragments arranged by size:
931 910 535 48
NheI G'CTAG_C
Cuts at: 0 369 2424
Size: 369 2055
PasI CC'CwG_GG
Cuts at: 0 871 2396 2424
Size: 871 1525 28
Fragments arranged by size:
1525 871 28
PflMI CCAn_nnn'nTGG
Cuts at: 0 1514 1539 1605 2396 2424
Size: 1514 25 66 791 28
Fragments arranged by size:
1514 791 66 28 25
PfoI T'CCnGG_A
Cuts at: 0 1649 2424
Size: 1649 775
PmlI CAC'GTG
Cuts at: 0 2159 2424
Size: 2159 265
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2155 2187 2424
Size: 2155 32 237
Fragments arranged by size:
2155 237 32
PpuMI rG'GwC_Cy
Cuts at: 0 666 2073 2318 2424
Size: 666 1407 245 106
Fragments arranged by size:
1407 666 245 106
PspOMI G'GGCC_C
Cuts at: 0 867 1030 1517 2424
Size: 867 163 487 907
Fragments arranged by size:
907 867 487 163
PstI C_TGCA'G
Cuts at: 0 548 980 1094 1409 1840 2424
Size: 548 432 114 315 431 584
Fragments arranged by size:
584 548 432 431 315 114
PvuII CAG'CTG
Cuts at: 0 93 756 793 1178 1536 1621 1661 1731
Size: 93 663 37 385 358 85 40 70
Cuts at: 1731 2424
Size: 693
Fragments arranged by size:
693 663 385 358 93 85 70 40
37
SacII CC_GC'GG
Cuts at: 0 14 2424
Size: 14 2410
SanDI GG'GwC_CC
Cuts at: 0 2073 2424
Size: 2073 351
SapI GCTCTTCn'nnn_
Cuts at: 0 508 2424
Size: 508 1916
SbfI CC_TGCA'GG
Cuts at: 0 1409 2424
Size: 1409 1015
SexAI A'CCwGG_T
Cuts at: 0 1598 2424
Size: 1598 826
SfcI C'TryA_G
Cuts at: 0 544 582 976 996 1090 1405 1836 2424
Size: 544 38 394 20 94 315 431 588
Fragments arranged by size:
588 544 431 394 315 94 38 20
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 163 334 343 2424
Size: 163 171 9 2081
Fragments arranged by size:
2081 171 163 9
SgrAI Cr'CCGG_yG
Cuts at: 0 910 1559 2424
Size: 910 649 865
Fragments arranged by size:
910 865 649
SmlI C'TyrA_G
Cuts at: 0 1666 2214 2269 2424
Size: 1666 548 55 155
Fragments arranged by size:
1666 548 155 55
StuI AGG'CCT
Cuts at: 0 776 2083 2424
Size: 776 1307 341
Fragments arranged by size:
1307 776 341
StyI C'CwwG_G
Cuts at: 0 341 801 1184 1592 2424
Size: 341 460 383 408 832
Fragments arranged by size:
832 460 408 383 341
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 748 1531 1921 2194 2424
Size: 748 783 390 273 230
Fragments arranged by size:
783 748 390 273 230
TatI w'GTAC_w
Cuts at: 0 779 845 2424
Size: 779 66 1579
Fragments arranged by size:
1579 779 66
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 422 454 2335 2367 2424
Size: 422 32 1881 32 57
Fragments arranged by size:
1881 422 57 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1819 2424
Size: 1819 605
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 45 1861 2424
Size: 45 1816 563
Fragments arranged by size:
1816 563 45
XmnI GAAnn'nnTTC
Cuts at: 0 985 2424
Size: 985 1439
ZraI GAC'GTC
Cuts at: 0 1097 2424
Size: 1097 1327
Enzymes that do cut:
AarI AatII Acc65I AcuI AfeI AgeI AhdI AleI
AlfI AlwNI ApaI ApaLI AvaI BanI BanII BbsI
BbvCI BcgI BglI BglII BlpI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRV
FalI HaeII Hin4I HincII KpnI MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV NheI PasI PflMI PfoI
PmlI PpiI PpuMI PspOMI PstI PvuII SacII SanDI
SapI SbfI SexAI SfcI SfiI SgrAI SmlI StuI
StyI TaqII TatI TstI Tth111I XcmI XmnI ZraI
Enzymes that do not cut:
AccI AclI AflII AflIII AloI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BciVI BclI BfrBI
BmgBI BsiWI BsmI BsmBI BspHI BstBI BstEII BstZ17I
BtgZI ClaI CspCI DraI EcoICRI EcoRI FseI FspI
FspAI HindIII HpaI KasI MfeI MluI NarI NdeI
NotI NruI NsiI NspI PacI PciI PmeI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SalI ScaI
SfoI SgfI SmaI SnaBI SpeI SphI SrfI SspI
SwaI XbaI XhoI XmaI