Restriction map for
pF1KB3903
(Linear) (Six-base) MAPSORT of: KIBB3903.seq Check: 7858 from: 1 to: 2298
KIBB3903.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AccI GT'mk_AC
Cuts at: 0 1450 2298
Size: 1450 848
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 892 941 1148 1729 1879 2298
Size: 892 49 207 581 150 419
Fragments arranged by size:
892 581 419 207 150 49
AfeI AGC'GCT
Cuts at: 0 527 2298
Size: 527 1771
AflIII A'CryG_T
Cuts at: 0 431 2298
Size: 431 1867
AleI CACnn'nnGTG
Cuts at: 0 1669 2298
Size: 1669 629
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1079 2298
Size: 1079 1219
AlwNI CAG_nnn'CTG
Cuts at: 0 1387 2298
Size: 1387 911
ApaI G_GGCC'C
Cuts at: 0 81 115 932 2298
Size: 81 34 817 1366
Fragments arranged by size:
1366 817 81 34
ApaLI G'TGCA_C
Cuts at: 0 1534 2298
Size: 1534 764
ApoI r'AATT_y
Cuts at: 0 817 2298
Size: 817 1481
AseI AT'TA_AT
Cuts at: 0 482 1879 2298
Size: 482 1397 419
Fragments arranged by size:
1397 482 419
AvaI C'yCGr_G
Cuts at: 0 108 521 2256 2298
Size: 108 413 1735 42
Fragments arranged by size:
1735 413 108 42
BamHI G'GATC_C
Cuts at: 0 638 913 2298
Size: 638 275 1385
Fragments arranged by size:
1385 638 275
BanI G'GyrC_C
Cuts at: 0 2282 2298
Size: 2282 16
BanII G_rGCy'C
Cuts at: 0 81 115 131 317 932 1394 2150 2298
Size: 81 34 16 186 615 462 756 148
Fragments arranged by size:
756 615 462 186 148 81 34 16
BbeI G_GCGC'C
Cuts at: 0 2286 2298
Size: 2286 12
BbvCI CC'TCA_GC
Cuts at: 0 193 894 1525 1988 2298
Size: 193 701 631 463 310
Fragments arranged by size:
701 631 463 310 193
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1427 1461 2298
Size: 1427 34 837
Fragments arranged by size:
1427 837 34
BclI T'GATC_A
Cuts at: 0 1736 2298
Size: 1736 562
BglI GCCn_nnn'nGGC
Cuts at: 0 217 2298
Size: 217 2081
BglII A'GATC_T
Cuts at: 0 1075 1425 1797 1811 2298
Size: 1075 350 372 14 487
Fragments arranged by size:
1075 487 372 350 14
Bme1580I G_kGCm'C
Cuts at: 0 81 115 156 932 1538 2298
Size: 81 34 41 776 606 760
Fragments arranged by size:
776 760 606 81 41 34
BmrI ACTGGGnnnn_n'
Cuts at: 0 131 1612 1857 2298
Size: 131 1481 245 441
Fragments arranged by size:
1481 441 245 131
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2169 2298
Size: 2169 129
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 258 996 1661 1774 1849 2141 2298
Size: 46 212 738 665 113 75 292 157
Fragments arranged by size:
738 665 292 212 157 113 75 46
Bpu10I CC'TnA_GC
Cuts at: 0 193 894 1103 1525 1988 2298
Size: 193 701 209 422 463 310
Fragments arranged by size:
701 463 422 310 209 193
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1192 2298
Size: 1192 1106
BsaI GGTCTCn'nnnn_
Cuts at: 0 2064 2298
Size: 2064 234
BsaAI yAC'GTr
Cuts at: 0 432 2298
Size: 432 1866
BsaHI Gr'CG_yC
Cuts at: 0 2283 2298
Size: 2283 15
BsaWI w'CCGG_w
Cuts at: 0 230 287 2220 2298
Size: 230 57 1933 78
Fragments arranged by size:
1933 230 78 57
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 78 295 531 1384 2191 2298
Size: 78 217 236 853 807 107
Fragments arranged by size:
853 807 236 217 107 78
BseYI C'CCAG_C
Cuts at: 0 82 973 1819 2298
Size: 82 891 846 479
Fragments arranged by size:
891 846 479 82
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 720 2298
Size: 720 1578
BsiEI CG_ry'CG
Cuts at: 0 61 282 2298
Size: 61 221 2016
Fragments arranged by size:
2016 221 61
BsiHKAI G_wGCw'C
Cuts at: 0 32 317 1394 1538 2298
Size: 32 285 1077 144 760
Fragments arranged by size:
1077 760 285 144 32
Bsp1286I G_dGCh'C
Cuts at: 0 32 81 115 131 156 317 932 1394
Size: 32 49 34 16 25 161 615 462
Cuts at: 1394 1538 2150 2298
Size: 144 612 148
Fragments arranged by size:
615 612 462 161 148 144 49 34
32 25 16
BspEI T'CCGG_A
Cuts at: 0 2220 2298
Size: 2220 78
BspHI T'CATG_A
Cuts at: 0 2069 2298
Size: 2069 229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 353 744 2298
Size: 353 391 1554
Fragments arranged by size:
1554 391 353
BsrBI CCG'CTC
Cuts at: 0 323 2268 2298
Size: 323 1945 30
Fragments arranged by size:
1945 323 30
BsrDI GCAATG_nn'
Cuts at: 0 1685 2298
Size: 1685 613
BsrGI T'GTAC_A
Cuts at: 0 1487 2298
Size: 1487 811
BssHII G'CGCG_C
Cuts at: 0 220 2298
Size: 220 2078
BstBI TT'CG_AA
Cuts at: 0 606 2298
Size: 606 1692
BstEII G'GTnAC_C
Cuts at: 0 393 2298
Size: 393 1905
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1671 2298
Size: 1671 627
BstYI r'GATC_y
Cuts at: 0 638 913 1075 1425 1797 1811 2180 2298
Size: 638 275 162 350 372 14 369 118
Fragments arranged by size:
638 372 369 350 275 162 118 14
Bsu36I CC'TnA_GG
Cuts at: 0 1507 2298
Size: 1507 791
BtgI C'CryG_G
Cuts at: 0 55 1481 1518 1922 2298
Size: 55 1426 37 404 376
Fragments arranged by size:
1426 404 376 55 37
BtsI GCAGTG_nn'
Cuts at: 0 156 1482 2298
Size: 156 1326 816
Fragments arranged by size:
1326 816 156
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1272 1307 2298
Size: 1272 35 991
Fragments arranged by size:
1272 991 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 718 2298
Size: 718 1580
EaeI y'GGCC_r
Cuts at: 0 2 58 2298
Size: 2 56 2240
Fragments arranged by size:
2240 56 2
EagI C'GGCC_G
Cuts at: 0 58 2298
Size: 58 2240
EarI CTCTTCn'nnn_
Cuts at: 0 1172 1807 2298
Size: 1172 635 491
Fragments arranged by size:
1172 635 491
EcoICRI GAG'CTC
Cuts at: 0 315 1392 2298
Size: 315 1077 906
Fragments arranged by size:
1077 906 315
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 258 892 941 996 1148 1661 1729
Size: 46 212 634 49 55 152 513 68
Cuts at: 1729 1774 1849 1879 2141 2298
Size: 45 75 30 262 157
Fragments arranged by size:
634 513 262 212 157 152 75 68
55 49 46 45 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 349 2298
Size: 349 1949
EcoO109I rG'GnC_Cy
Cuts at: 0 77 78 112 189 255 929 1636 2298
Size: 77 1 34 77 66 674 707 662
Fragments arranged by size:
707 674 662 77 77 66 34 1
EcoRV GAT'ATC
Cuts at: 0 690 2298
Size: 690 1608
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 240 2298
Size: 240 2058
HaeII r_GCGC'y
Cuts at: 0 529 2286 2298
Size: 529 1757 12
Fragments arranged by size:
1757 529 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1050 1082 1948 1980 2298
Size: 1050 32 866 32 318
Fragments arranged by size:
1050 866 318 32 32
HincII GTy'rAC
Cuts at: 0 1451 2298
Size: 1451 847
KasI G'GCGC_C
Cuts at: 0 2282 2298
Size: 2282 16
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 1048 2298
Size: 311 737 1250
Fragments arranged by size:
1250 737 311
MscI TGG'CCA
Cuts at: 0 4 2298
Size: 4 2294
MslI CAynn'nnrTG
Cuts at: 0 1669 2298
Size: 1669 629
MspA1I CmG'CkG
Cuts at: 0 57 898 1729 1924 2129 2251 2298
Size: 57 841 831 195 205 122 47
Fragments arranged by size:
841 831 205 195 122 57 47
NarI GG'CG_CC
Cuts at: 0 2283 2298
Size: 2283 15
NcoI C'CATG_G
Cuts at: 0 1481 1518 2298
Size: 1481 37 780
Fragments arranged by size:
1481 780 37
NspI r_CATG'y
Cuts at: 0 806 1983 2298
Size: 806 1177 315
Fragments arranged by size:
1177 806 315
PasI CC'CwG_GG
Cuts at: 0 351 925 1946 2298
Size: 351 574 1021 352
Fragments arranged by size:
1021 574 352 351
PflMI CCAn_nnn'nTGG
Cuts at: 0 1815 2298
Size: 1815 483
PfoI T'CCnGG_A
Cuts at: 0 845 1032 1790 2298
Size: 845 187 758 508
Fragments arranged by size:
845 758 508 187
PspOMI G'GGCC_C
Cuts at: 0 77 111 928 2298
Size: 77 34 817 1370
Fragments arranged by size:
1370 817 77 34
PstI C_TGCA'G
Cuts at: 0 163 188 2110 2298
Size: 163 25 1922 188
Fragments arranged by size:
1922 188 163 25
PvuII CAG'CTG
Cuts at: 0 898 1729 2298
Size: 898 831 569
Fragments arranged by size:
898 831 569
SacI G_AGCT'C
Cuts at: 0 317 1394 2298
Size: 317 1077 904
Fragments arranged by size:
1077 904 317
SacII CC_GC'GG
Cuts at: 0 58 1925 2298
Size: 58 1867 373
Fragments arranged by size:
1867 373 58
SalI G'TCGA_C
Cuts at: 0 1449 2298
Size: 1449 849
SbfI CC_TGCA'GG
Cuts at: 0 188 2298
Size: 188 2110
SfcI C'TryA_G
Cuts at: 0 159 184 2106 2298
Size: 159 25 1922 192
Fragments arranged by size:
1922 192 159 25
SfoI GGC'GCC
Cuts at: 0 2284 2298
Size: 2284 14
SmaI CCC'GGG
Cuts at: 0 2258 2298
Size: 2258 40
SmlI C'TyrA_G
Cuts at: 0 1171 2298
Size: 1171 1127
SpeI A'CTAG_T
Cuts at: 0 1027 2298
Size: 1027 1271
SspI AAT'ATT
Cuts at: 0 480 2298
Size: 480 1818
StuI AGG'CCT
Cuts at: 0 2136 2298
Size: 2136 162
StyI C'CwwG_G
Cuts at: 0 116 1481 1518 1871 2298
Size: 116 1365 37 353 427
Fragments arranged by size:
1365 427 353 116 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1677 1938 2298
Size: 1677 261 360
Fragments arranged by size:
1677 360 261
TatI w'GTAC_w
Cuts at: 0 1457 1487 2298
Size: 1457 30 811
Fragments arranged by size:
1457 811 30
Tth111I GACn'n_nGTC
Cuts at: 0 841 2298
Size: 841 1457
XbaI T'CTAG_A
Cuts at: 0 2082 2298
Size: 2082 216
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1816 2298
Size: 1816 482
XmaI C'CCGG_G
Cuts at: 0 2256 2298
Size: 2256 42
XmnI GAAnn'nnTTC
Cuts at: 0 452 818 2298
Size: 452 366 1480
Fragments arranged by size:
1480 452 366
Enzymes that do cut:
AccI AcuI AfeI AflIII AleI AlfI AlwNI ApaI
ApaLI ApoI AseI AvaI BamHI BanI BanII BbeI
BbvCI BcgI BclI BglI BglII Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrGI BssHII BstBI BstEII BstXI BstYI
Bsu36I BtgI BtsI CspCI DrdI EaeI EagI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I
HincII KasI MmeI MscI MslI MspA1I NarI NcoI
NspI PasI PflMI PfoI PspOMI PstI PvuII SacI
SacII SalI SbfI SfcI SfoI SmaI SmlI SpeI
SspI StuI StyI TaqII TatI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AloI
AscI AsiSI AvrII BaeI BbsI BciVI BfrBI BlpI
BmgBI BmtI BsaBI BsaXI BsiWI BsmI BsmBI BsrFI
BssSI BstAPI BstZ17I BtgZI ClaI DraI DraIII EciI
EcoRI FseI FspI FspAI HindIII HpaI KpnI MfeI
MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI RsrII SanDI SapI ScaI SexAI
SfiI SgfI SgrAI SnaBI SphI SrfI SwaI TstI
XhoI ZraI