(Linear) (Six-base) MAPSORT of: KIBB3912.seq  Check: 4273  from: 1  to: 459



KIBB3912.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:25  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     459 
   Size:        169     290
 
BanI G'GyrC_C

Cuts at:      0     255     459 
   Size:        255     204
 
BbeI G_GCGC'C

Cuts at:      0     259     459 
   Size:        259     200
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      10     459 
   Size:         10     449
 
BlpI GC'TnA_GC

Cuts at:      0     167     459 
   Size:        167     292
 
Bme1580I G_kGCm'C

Cuts at:      0     369     459 
   Size:        369      90
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     275     459 
   Size:        275     184
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     225     334     459 
   Size:        225     109     125

  Fragments arranged by size: 

                 225     125     109
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     319     459 
   Size:        319     140
 
BsaHI Gr'CG_yC

Cuts at:      0     256     459 
   Size:        256     203
 
BsaWI w'CCGG_w

Cuts at:      0     147     290     459 
   Size:        147     143     169

  Fragments arranged by size: 

                 169     147     143
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      75     459 
   Size:         75     384
 
BseYI C'CCAG_C

Cuts at:      0     368     459 
   Size:        368      91
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     455     459 
   Size:        455       4
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     145     459 
   Size:        145     314
 
Bsp1286I G_dGCh'C

Cuts at:      0     369     459 
   Size:        369      90
 
BspEI T'CCGG_A

Cuts at:      0     290     459 
   Size:        290     169
 
BtgI C'CryG_G

Cuts at:      0     137     459 
   Size:        137     322
 
EarI CTCTTCn'nnn_

Cuts at:      0     100     188     459 
   Size:        100      88     271

  Fragments arranged by size: 

                 271     100      88
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     225     334     459 
   Size:        169      56     109     125

  Fragments arranged by size: 

                 169     125     109      56
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     263     459 
   Size:        263     196
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      60     459 
   Size:         60     399
 
FspI TGC'GCA

Cuts at:      0     327     459 
   Size:        327     132
 
HaeII r_GCGC'y

Cuts at:      0     109     259     375     459 
   Size:        109     150     116      84

  Fragments arranged by size: 

                 150     116     109      84
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     285     317     459 
   Size:        285      32     142

  Fragments arranged by size: 

                 285     142      32
 
KasI G'GCGC_C

Cuts at:      0     255     459 
   Size:        255     204
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     229     459 
   Size:         46     183     230

  Fragments arranged by size: 

                 230     183      46
 
MslI CAynn'nnrTG

Cuts at:      0     382     459 
   Size:        382      77
 
NarI GG'CG_CC

Cuts at:      0     256     459 
   Size:        256     203
 
NspI r_CATG'y

Cuts at:      0     387     459 
   Size:        387      72
 
SapI GCTCTTCn'nnn_

Cuts at:      0     188     459 
   Size:        188     271
 
SfcI C'TryA_G

Cuts at:      0     450     459 
   Size:        450       9
 
SfoI GGC'GCC

Cuts at:      0     257     459 
   Size:        257     202
 
SmlI C'TyrA_G

Cuts at:      0     334     459 
   Size:        334     125
 
TatI w'GTAC_w

Cuts at:      0     405     459 
   Size:        405      54
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      64      96     459 
   Size:         64      32     363

  Fragments arranged by size: 

                 363      64      32
 

 Enzymes that do cut:

     AcuI     BanI     BbeI     BbsI     BlpI Bme1580I     BplI     BpmI
    BpuEI    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsmBI Bsp1286I
    BspEI     BtgI     EarI  Eco57MI    EcoNI     FalI     FspI    HaeII
    Hin4I     KasI     MmeI     MslI     NarI     NspI     SapI     SfcI
     SfoI     SmlI     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
    BanII    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
    BmgBI     BmrI     BmtI   Bpu10I     BsaI    BsaAI    BsaBI    BsaXI
    BsiEI  BsiHKAI    BsiWI     BsmI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI EcoO109I    EcoRI
    EcoRV     FseI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     MscI   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI