Restriction map for
pF1KB3935
(Linear) (Six-base) MAPSORT of: KIBB3935.seq Check: 7007 from: 1 to: 1266
KIBB3935.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1059 1266
Size: 1059 207
Acc65I G'GTAC_C
Cuts at: 0 642 1266
Size: 642 624
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 421 919 991 1266
Size: 148 273 498 72 275
Fragments arranged by size:
498 275 273 148 72
AlwNI CAG_nnn'CTG
Cuts at: 0 477 1266
Size: 477 789
ApoI r'AATT_y
Cuts at: 0 158 577 727 1266
Size: 158 419 150 539
Fragments arranged by size:
539 419 158 150
BanI G'GyrC_C
Cuts at: 0 642 1266
Size: 642 624
BbsI GAAGACnn'nnnn_
Cuts at: 0 340 609 1266
Size: 340 269 657
Fragments arranged by size:
657 340 269
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 45 79 1266
Size: 45 34 1187
Fragments arranged by size:
1187 45 34
BfrBI ATG'CAT
Cuts at: 0 115 1266
Size: 115 1151
BglI GCCn_nnn'nGGC
Cuts at: 0 1252 1266
Size: 1252 14
Bme1580I G_kGCm'C
Cuts at: 0 529 1266
Size: 529 737
BmrI ACTGGGnnnn_n'
Cuts at: 0 436 1203 1266
Size: 436 767 63
Fragments arranged by size:
767 436 63
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 904 1266
Size: 116 788 362
Fragments arranged by size:
788 362 116
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 1266
Size: 340 926
BsaAI yAC'GTr
Cuts at: 0 543 849 1266
Size: 543 306 417
Fragments arranged by size:
543 417 306
BsaBI GATnn'nnATC
Cuts at: 0 695 1266
Size: 695 571
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 433 1246 1266
Size: 433 813 20
Fragments arranged by size:
813 433 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1087 1266
Size: 1087 179
BsmI GAATG_Cn'
Cuts at: 0 303 1266
Size: 303 963
Bsp1286I G_dGCh'C
Cuts at: 0 529 1266
Size: 529 737
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 229 794 1059 1215 1266
Size: 229 565 265 156 51
Fragments arranged by size:
565 265 229 156 51
BsrDI GCAATG_nn'
Cuts at: 0 78 163 1266
Size: 78 85 1103
Fragments arranged by size:
1103 85 78
BsrFI r'CCGG_y
Cuts at: 0 1150 1266
Size: 1150 116
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1221 1266
Size: 1221 45
BtgI C'CryG_G
Cuts at: 0 172 1266
Size: 172 1094
DraI TTT'AAA
Cuts at: 0 732 1266
Size: 732 534
EaeI y'GGCC_r
Cuts at: 0 759 1184 1253 1266
Size: 759 425 69 13
Fragments arranged by size:
759 425 69 13
EarI CTCTTCn'nnn_
Cuts at: 0 410 800 1266
Size: 410 390 466
Fragments arranged by size:
466 410 390
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 148 421 904 919 991 1266
Size: 116 32 273 483 15 72 275
Fragments arranged by size:
483 275 273 116 72 32 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 98 1266
Size: 98 1168
EcoO109I rG'GnC_Cy
Cuts at: 0 24 351 1266
Size: 24 327 915
Fragments arranged by size:
915 327 24
EcoRV GAT'ATC
Cuts at: 0 828 1266
Size: 828 438
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 29 330 362 688 720 1266
Size: 29 301 32 326 32 546
Fragments arranged by size:
546 326 301 32 32 29
KpnI G_GTAC'C
Cuts at: 0 646 1266
Size: 646 620
MscI TGG'CCA
Cuts at: 0 761 1186 1266
Size: 761 425 80
Fragments arranged by size:
761 425 80
MslI CAynn'nnrTG
Cuts at: 0 307 1266
Size: 307 959
MspA1I CmG'CkG
Cuts at: 0 474 1266
Size: 474 792
NruI TCG'CGA
Cuts at: 0 55 1266
Size: 55 1211
NsiI A_TGCA'T
Cuts at: 0 117 1266
Size: 117 1149
NspI r_CATG'y
Cuts at: 0 1182 1266
Size: 1182 84
PflMI CCAn_nnn'nTGG
Cuts at: 0 477 1266
Size: 477 789
PfoI T'CCnGG_A
Cuts at: 0 93 1266
Size: 93 1173
PshAI GACnn'nnGTC
Cuts at: 0 589 874 1170 1266
Size: 589 285 296 96
Fragments arranged by size:
589 296 285 96
PstI C_TGCA'G
Cuts at: 0 1226 1266
Size: 1226 40
PvuII CAG'CTG
Cuts at: 0 474 1266
Size: 474 792
SapI GCTCTTCn'nnn_
Cuts at: 0 800 1266
Size: 800 466
SbfI CC_TGCA'GG
Cuts at: 0 1226 1266
Size: 1226 40
SfcI C'TryA_G
Cuts at: 0 1222 1266
Size: 1222 44
SgrAI Cr'CCGG_yG
Cuts at: 0 1150 1266
Size: 1150 116
SmlI C'TyrA_G
Cuts at: 0 355 1266
Size: 355 911
SnaBI TAC'GTA
Cuts at: 0 849 1266
Size: 849 417
StyI C'CwwG_G
Cuts at: 0 512 1266
Size: 512 754
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 649 811 904 1266
Size: 649 162 93 362
Fragments arranged by size:
649 362 162 93
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 519 551 1266
Size: 519 32 715
Fragments arranged by size:
715 519 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 170 1266
Size: 170 1096
Enzymes that do cut:
AarI Acc65I AcuI AlwNI ApoI BanI BbsI BcgI
BfrBI BglI Bme1580I BmrI BpmI BpuEI BsaAI BsaBI
BseRI BsgI BsmI Bsp1286I BspMI BsrDI BsrFI BstAPI
BtgI DraI EaeI EarI Eco57MI EcoNI EcoO109I EcoRV
Hin4I KpnI MscI MslI MspA1I NruI NsiI NspI
PflMI PfoI PshAI PstI PvuII SapI SbfI SfcI
SgrAI SmlI SnaBI StyI TaqII TstI XcmI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanII BbeI BbvCI BciVI
BclI BglII BlpI BmgBI BmtI BplI Bpu10I BsaI
BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsiWI BsmBI
BspEI BspHI BsrBI BsrGI BssHII BssSI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraIII DrdI EagI EciI EcoICRI EcoRI FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI MfeI
MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI
NotI PacI PasI PciI PmeI PmlI PpiI PpuMI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI ScaI SexAI SfiI SfoI SgfI SmaI
SpeI SphI SrfI SspI StuI SwaI TatI Tth111I
XbaI XhoI XmaI XmnI ZraI