Restriction map for
pF1KB3947
(Linear) (Six-base) MAPSORT of: KIBB3947.seq Check: 5046 from: 1 to: 2664
KIBB3947.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 0 1107 1344 1890 2664
Size: 1107 237 546 774
Fragments arranged by size:
1107 774 546 237
Acc65I G'GTAC_C
Cuts at: 0 544 2664
Size: 544 2120
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1856 2664
Size: 1856 808
AflIII A'CryG_T
Cuts at: 0 104 2664
Size: 104 2560
AhdI GACnn_n'nnGTC
Cuts at: 0 2584 2664
Size: 2584 80
AlwNI CAG_nnn'CTG
Cuts at: 0 815 2664
Size: 815 1849
ApoI r'AATT_y
Cuts at: 0 243 1378 1648 2205 2664
Size: 243 1135 270 557 459
Fragments arranged by size:
1135 557 459 270 243
AvaI C'yCGr_G
Cuts at: 0 668 716 2664
Size: 668 48 1948
Fragments arranged by size:
1948 668 48
BanI G'GyrC_C
Cuts at: 0 544 1732 2664
Size: 544 1188 932
Fragments arranged by size:
1188 932 544
BanII G_rGCy'C
Cuts at: 0 2218 2664
Size: 2218 446
BbsI GAAGACnn'nnnn_
Cuts at: 0 1777 2662 2664
Size: 1777 885 2
Fragments arranged by size:
1777 885 2
BbvCI CC'TCA_GC
Cuts at: 0 2438 2664
Size: 2438 226
BclI T'GATC_A
Cuts at: 0 944 2664
Size: 944 1720
BfrBI ATG'CAT
Cuts at: 0 24 209 2664
Size: 24 185 2455
Fragments arranged by size:
2455 185 24
BglII A'GATC_T
Cuts at: 0 125 2664
Size: 125 2539
BlpI GC'TnA_GC
Cuts at: 0 703 1418 2664
Size: 703 715 1246
Fragments arranged by size:
1246 715 703
BmgBI CAC'GTC
Cuts at: 0 1111 1888 2664
Size: 1111 777 776
Fragments arranged by size:
1111 777 776
BmrI ACTGGGnnnn_n'
Cuts at: 0 1768 2664
Size: 1768 896
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1510 2664
Size: 1510 1154
Bpu10I CC'TnA_GC
Cuts at: 0 2438 2664
Size: 2438 226
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 288 2664
Size: 288 2376
BsaAI yAC'GTr
Cuts at: 0 1792 2664
Size: 1792 872
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2618 2648 2664
Size: 2618 30 16
Fragments arranged by size:
2618 30 16
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 824 1248 2664
Size: 824 424 1416
Fragments arranged by size:
1416 824 424
BseYI C'CCAG_C
Cuts at: 0 636 1428 2227 2542 2664
Size: 636 792 799 315 122
Fragments arranged by size:
799 792 636 315 122
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2367 2664
Size: 2367 297
BsmI GAATG_Cn'
Cuts at: 0 26 1365 2664
Size: 26 1339 1299
Fragments arranged by size:
1339 1299 26
Bsp1286I G_dGCh'C
Cuts at: 0 2218 2664
Size: 2218 446
BspHI T'CATG_A
Cuts at: 0 623 1461 2664
Size: 623 838 1203
Fragments arranged by size:
1203 838 623
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2085 2664
Size: 2085 579
BsrDI GCAATG_nn'
Cuts at: 0 2282 2664
Size: 2282 382
BssSI C'ACGA_G
Cuts at: 0 473 1766 2014 2664
Size: 473 1293 248 650
Fragments arranged by size:
1293 650 473 248
BstAPI GCAn_nnn'nTGC
Cuts at: 0 427 2664
Size: 427 2237
BstBI TT'CG_AA
Cuts at: 0 18 2664
Size: 18 2646
BstYI r'GATC_y
Cuts at: 0 125 2664
Size: 125 2539
BtgI C'CryG_G
Cuts at: 0 1961 2222 2664
Size: 1961 261 442
Fragments arranged by size:
1961 442 261
BtsI GCAGTG_nn'
Cuts at: 0 183 1853 2664
Size: 183 1670 811
Fragments arranged by size:
1670 811 183
EaeI y'GGCC_r
Cuts at: 0 1688 2664
Size: 1688 976
EarI CTCTTCn'nnn_
Cuts at: 0 155 1154 2097 2664
Size: 155 999 943 567
Fragments arranged by size:
999 943 567 155
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1510 1856 2664
Size: 1510 346 808
Fragments arranged by size:
1510 808 346
EcoO109I rG'GnC_Cy
Cuts at: 0 493 1624 2664
Size: 493 1131 1040
Fragments arranged by size:
1131 1040 493
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1026 1699 2664
Size: 1026 673 965
Fragments arranged by size:
1026 965 673
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 765 797 810 842 1018 1050 1261 1293
Size: 765 32 13 32 176 32 211 32
Cuts at: 1293 1443 1475 2570 2602 2664
Size: 150 32 1095 32 62
Fragments arranged by size:
1095 765 211 176 150 62 32 32
32 32 32 32 13
HincII GTy'rAC
Cuts at: 0 253 2193 2664
Size: 253 1940 471
Fragments arranged by size:
1940 471 253
HindIII A'AGCT_T
Cuts at: 0 368 1504 2664
Size: 368 1136 1160
Fragments arranged by size:
1160 1136 368
KpnI G_GTAC'C
Cuts at: 0 548 2664
Size: 548 2116
MfeI C'AATT_G
Cuts at: 0 1098 2664
Size: 1098 1566
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 464 1421 1593 2664
Size: 115 349 957 172 1071
Fragments arranged by size:
1071 957 349 172 115
MscI TGG'CCA
Cuts at: 0 1690 2664
Size: 1690 974
MslI CAynn'nnrTG
Cuts at: 0 88 103 1466 1966 2664
Size: 88 15 1363 500 698
Fragments arranged by size:
1363 698 500 88 15
MspA1I CmG'CkG
Cuts at: 0 2381 2664
Size: 2381 283
NcoI C'CATG_G
Cuts at: 0 1961 2222 2664
Size: 1961 261 442
Fragments arranged by size:
1961 442 261
NsiI A_TGCA'T
Cuts at: 0 26 211 2664
Size: 26 185 2453
Fragments arranged by size:
2453 185 26
NspI r_CATG'y
Cuts at: 0 108 597 2480 2664
Size: 108 489 1883 184
Fragments arranged by size:
1883 489 184 108
PasI CC'CwG_GG
Cuts at: 0 50 1316 2664
Size: 50 1266 1348
Fragments arranged by size:
1348 1266 50
PciI A'CATG_T
Cuts at: 0 104 2664
Size: 104 2560
PmlI CAC'GTG
Cuts at: 0 1792 2664
Size: 1792 872
PpuMI rG'GwC_Cy
Cuts at: 0 493 1624 2664
Size: 493 1131 1040
Fragments arranged by size:
1131 1040 493
PsiI TTA'TAA
Cuts at: 0 187 1198 1550 2664
Size: 187 1011 352 1114
Fragments arranged by size:
1114 1011 352 187
PstI C_TGCA'G
Cuts at: 0 1711 1809 2664
Size: 1711 98 855
Fragments arranged by size:
1711 855 98
PvuII CAG'CTG
Cuts at: 0 2381 2664
Size: 2381 283
ScaI AGT'ACT
Cuts at: 0 1283 2664
Size: 1283 1381
SfcI C'TryA_G
Cuts at: 0 1070 1345 1707 1805 1891 2396 2664
Size: 1070 275 362 98 86 505 268
Fragments arranged by size:
1070 505 362 275 268 98 86
SmlI C'TyrA_G
Cuts at: 0 303 2664
Size: 303 2361
SphI G_CATG'C
Cuts at: 0 2480 2664
Size: 2480 184
SspI AAT'ATT
Cuts at: 0 468 2664
Size: 468 2196
StyI C'CwwG_G
Cuts at: 0 650 1559 1961 2141 2222 2637 2664
Size: 650 909 402 180 81 415 27
Fragments arranged by size:
909 650 415 402 180 81 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2404 2664
Size: 2404 260
TatI w'GTAC_w
Cuts at: 0 319 844 1004 1281 2062 2664
Size: 319 525 160 277 781 602
Fragments arranged by size:
781 602 525 319 277 160
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1752 1784 2664
Size: 1752 32 880
Fragments arranged by size:
1752 880 32
Enzymes that do cut:
AccI Acc65I AcuI AflIII AhdI AlwNI ApoI AvaI
BanI BanII BbsI BbvCI BclI BfrBI BglII BlpI
BmgBI BmrI BpmI Bpu10I BpuEI BsaAI BsaXI BseRI
BseYI BsgI BsmI Bsp1286I BspHI BspMI BsrDI BssSI
BstAPI BstBI BstYI BtgI BtsI EaeI EarI Eco57MI
EcoO109I FalI Hin4I HincII HindIII KpnI MfeI MmeI
MscI MslI MspA1I NcoI NsiI NspI PasI PciI
PmlI PpuMI PsiI PstI PvuII ScaI SfcI SmlI
SphI SspI StyI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AleI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BcgI BciVI BglI Bme1580I BmtI BplI
BsaI BsaBI BsaHI BsaWI BsiEI BsiHKAI BsiWI BsmBI
BspEI BsrBI BsrFI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
HaeII HpaI KasI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PflMI PfoI PmeI PpiI
PshAI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI StuI SwaI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI