Restriction map for
pF1KB3951
(Linear) (Six-base) MAPSORT of: KIBB3951.seq Check: 4037 from: 1 to: 915
KIBB3951.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AccI GT'mk_AC
Cuts at: 0 248 348 915
Size: 248 100 567
Fragments arranged by size:
567 248 100
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 617 915
Size: 91 526 298
Fragments arranged by size:
526 298 91
AflIII A'CryG_T
Cuts at: 0 876 915
Size: 876 39
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 70 915
Size: 70 845
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 194 226 915
Size: 194 32 689
Fragments arranged by size:
689 194 32
AlwNI CAG_nnn'CTG
Cuts at: 0 595 915
Size: 595 320
ApaLI G'TGCA_C
Cuts at: 0 718 915
Size: 718 197
ApoI r'AATT_y
Cuts at: 0 524 551 733 915
Size: 524 27 182 182
Fragments arranged by size:
524 182 182 27
AvaI C'yCGr_G
Cuts at: 0 626 915
Size: 626 289
BanI G'GyrC_C
Cuts at: 0 3 915
Size: 3 912
BbeI G_GCGC'C
Cuts at: 0 7 915
Size: 7 908
BbsI GAAGACnn'nnnn_
Cuts at: 0 810 915
Size: 810 105
BciVI GTATCCnnnnn_n'
Cuts at: 0 600 915
Size: 600 315
Bme1580I G_kGCm'C
Cuts at: 0 722 915
Size: 722 193
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 221 435 915
Size: 221 214 480
Fragments arranged by size:
480 221 214
BsaI GGTCTCn'nnnn_
Cuts at: 0 265 915
Size: 265 650
BsaHI Gr'CG_yC
Cuts at: 0 4 915
Size: 4 911
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 194 224 408 438 641 671 915
Size: 194 30 184 30 203 30 244
Fragments arranged by size:
244 203 194 184 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 91 644 915
Size: 91 553 271
Fragments arranged by size:
553 271 91
BsiHKAI G_wGCw'C
Cuts at: 0 708 722 915
Size: 708 14 193
Fragments arranged by size:
708 193 14
Bsp1286I G_dGCh'C
Cuts at: 0 708 722 915
Size: 708 14 193
Fragments arranged by size:
708 193 14
BspHI T'CATG_A
Cuts at: 0 339 915
Size: 339 576
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 62 625 915
Size: 62 563 290
Fragments arranged by size:
563 290 62
BstBI TT'CG_AA
Cuts at: 0 528 915
Size: 528 387
BstYI r'GATC_y
Cuts at: 0 591 915
Size: 591 324
BtgI C'CryG_G
Cuts at: 0 162 486 915
Size: 162 324 429
Fragments arranged by size:
429 324 162
DrdI GACnn_nn'nnGTC
Cuts at: 0 765 915
Size: 765 150
EarI CTCTTCn'nnn_
Cuts at: 0 104 915
Size: 104 811
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 221 435 617 915
Size: 91 130 214 182 298
Fragments arranged by size:
298 214 182 130 91
EcoNI CCTnn'n_nnAGG
Cuts at: 0 560 915
Size: 560 355
EcoRV GAT'ATC
Cuts at: 0 159 507 915
Size: 159 348 408
Fragments arranged by size:
408 348 159
HaeII r_GCGC'y
Cuts at: 0 7 915
Size: 7 908
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 641 673 915
Size: 641 32 242
Fragments arranged by size:
641 242 32
KasI G'GCGC_C
Cuts at: 0 3 915
Size: 3 912
MslI CAynn'nnrTG
Cuts at: 0 232 915
Size: 232 683
NarI GG'CG_CC
Cuts at: 0 4 915
Size: 4 911
NcoI C'CATG_G
Cuts at: 0 162 486 915
Size: 162 324 429
Fragments arranged by size:
429 324 162
NspI r_CATG'y
Cuts at: 0 444 915
Size: 444 471
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 194 226 915
Size: 194 32 689
Fragments arranged by size:
689 194 32
PstI C_TGCA'G
Cuts at: 0 703 915
Size: 703 212
SfcI C'TryA_G
Cuts at: 0 261 699 915
Size: 261 438 216
Fragments arranged by size:
438 261 216
SfoI GGC'GCC
Cuts at: 0 5 915
Size: 5 910
SpeI A'CTAG_T
Cuts at: 0 714 915
Size: 714 201
SspI AAT'ATT
Cuts at: 0 801 915
Size: 801 114
StyI C'CwwG_G
Cuts at: 0 162 486 915
Size: 162 324 429
Fragments arranged by size:
429 324 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 226 915
Size: 226 689
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 394 915
Size: 394 521
Enzymes that do cut:
AccI AcuI AflIII AlfI AloI AlwNI ApaLI ApoI
AvaI BanI BbeI BbsI BciVI Bme1580I BpmI BsaI
BsaHI BsaXI BseRI BsiHKAI Bsp1286I BspHI BspMI BstBI
BstYI BtgI DrdI EarI Eco57MI EcoNI EcoRV HaeII
Hin4I KasI MslI NarI NcoI NspI PpiI PstI
SfcI SfoI SpeI SspI StyI TaqII XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI ApaI AscI AseI AsiSI AvrII BaeI BamHI
BanII BbvCI BcgI BclI BfrBI BglI BglII BlpI
BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaAI BsaBI
BsaWI BseYI BsgI BsiEI BsiWI BsmI BsmBI BspEI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII
BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII EaeI EagI EciI EcoICRI EcoO109I EcoRI FalI
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MspA1I NaeI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI
SnaBI SphI SrfI StuI SwaI TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI