Restriction map for
pF1KB3955
(Linear) (Six-base) MAPSORT of: KIBB3955.seq Check: 1997 from: 1 to: 1362
KIBB3955.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AatII G_ACGT'C
Cuts at: 0 624 1362
Size: 624 738
AccI GT'mk_AC
Cuts at: 0 1163 1362
Size: 1163 199
Acc65I G'GTAC_C
Cuts at: 0 186 1362
Size: 186 1176
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 919 1055 1252 1362
Size: 919 136 197 110
Fragments arranged by size:
919 197 136 110
AflIII A'CryG_T
Cuts at: 0 1355 1362
Size: 1355 7
AleI CACnn'nnGTG
Cuts at: 0 430 1362
Size: 430 932
ApaI G_GGCC'C
Cuts at: 0 823 1362
Size: 823 539
ApoI r'AATT_y
Cuts at: 0 97 328 862 978 1015 1362
Size: 97 231 534 116 37 347
Fragments arranged by size:
534 347 231 116 97 37
BanI G'GyrC_C
Cuts at: 0 186 1362
Size: 186 1176
BanII G_rGCy'C
Cuts at: 0 823 1362
Size: 823 539
BbsI GAAGACnn'nnnn_
Cuts at: 0 1240 1362
Size: 1240 122
BclI T'GATC_A
Cuts at: 0 372 1362
Size: 372 990
BfrBI ATG'CAT
Cuts at: 0 451 1362
Size: 451 911
BglII A'GATC_T
Cuts at: 0 111 144 1362
Size: 111 33 1218
Fragments arranged by size:
1218 111 33
Bme1580I G_kGCm'C
Cuts at: 0 823 1362
Size: 823 539
BmrI ACTGGGnnnn_n'
Cuts at: 0 694 1362
Size: 694 668
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1169 1362
Size: 1169 193
Bpu10I CC'TnA_GC
Cuts at: 0 182 1362
Size: 182 1180
BsaAI yAC'GTr
Cuts at: 0 1356 1362
Size: 1356 6
BsaHI Gr'CG_yC
Cuts at: 0 621 1362
Size: 621 741
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 48 78 1362
Size: 48 30 1284
Fragments arranged by size:
1284 48 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 746 1276 1362
Size: 746 530 86
Fragments arranged by size:
746 530 86
BseYI C'CCAG_C
Cuts at: 0 495 1362
Size: 495 867
BsmI GAATG_Cn'
Cuts at: 0 1058 1362
Size: 1058 304
Bsp1286I G_dGCh'C
Cuts at: 0 823 1362
Size: 823 539
BspHI T'CATG_A
Cuts at: 0 120 1362
Size: 120 1242
BsrGI T'GTAC_A
Cuts at: 0 353 1362
Size: 353 1009
BstEII G'GTnAC_C
Cuts at: 0 36 1362
Size: 36 1326
BstYI r'GATC_y
Cuts at: 0 111 144 1362
Size: 111 33 1218
Fragments arranged by size:
1218 111 33
BtgI C'CryG_G
Cuts at: 0 542 719 1199 1362
Size: 542 177 480 163
Fragments arranged by size:
542 480 177 163
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 497 1362
Size: 497 865
BtsI GCAGTG_nn'
Cuts at: 0 439 735 1225 1362
Size: 439 296 490 137
Fragments arranged by size:
490 439 296 137
DraI TTT'AAA
Cuts at: 0 327 1020 1362
Size: 327 693 342
Fragments arranged by size:
693 342 327
EaeI y'GGCC_r
Cuts at: 0 734 1362
Size: 734 628
EarI CTCTTCn'nnn_
Cuts at: 0 965 1031 1362
Size: 965 66 331
Fragments arranged by size:
965 331 66
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 919 1055 1169 1252 1362
Size: 919 136 114 83 110
Fragments arranged by size:
919 136 114 110 83
EcoO109I rG'GnC_Cy
Cuts at: 0 398 819 820 907 1362
Size: 398 421 1 87 455
Fragments arranged by size:
455 421 398 87 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 254 1362
Size: 254 1108
HindIII A'AGCT_T
Cuts at: 0 744 1362
Size: 744 618
KpnI G_GTAC'C
Cuts at: 0 190 1362
Size: 190 1172
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 428 1362
Size: 428 934
MslI CAynn'nnrTG
Cuts at: 0 430 1289 1362
Size: 430 859 73
Fragments arranged by size:
859 430 73
NcoI C'CATG_G
Cuts at: 0 542 719 1199 1362
Size: 542 177 480 163
Fragments arranged by size:
542 480 177 163
NdeI CA'TA_TG
Cuts at: 0 792 1362
Size: 792 570
NsiI A_TGCA'T
Cuts at: 0 453 1362
Size: 453 909
NspI r_CATG'y
Cuts at: 0 1311 1362
Size: 1311 51
PpuMI rG'GwC_Cy
Cuts at: 0 907 1362
Size: 907 455
PspOMI G'GGCC_C
Cuts at: 0 819 1362
Size: 819 543
PstI C_TGCA'G
Cuts at: 0 310 1362
Size: 310 1052
SapI GCTCTTCn'nnn_
Cuts at: 0 965 1362
Size: 965 397
SfcI C'TryA_G
Cuts at: 0 306 1362
Size: 306 1056
StyI C'CwwG_G
Cuts at: 0 542 610 719 1199 1362
Size: 542 68 109 480 163
Fragments arranged by size:
542 480 163 109 68
TatI w'GTAC_w
Cuts at: 0 353 1362
Size: 353 1009
XmnI GAAnn'nnTTC
Cuts at: 0 137 1362
Size: 137 1225
ZraI GAC'GTC
Cuts at: 0 622 1362
Size: 622 740
Enzymes that do cut:
AatII AccI Acc65I AcuI AflIII AleI ApaI ApoI
BanI BanII BbsI BclI BfrBI BglII Bme1580I BmrI
BpmI Bpu10I BsaAI BsaHI BsaXI BseRI BseYI BsmI
Bsp1286I BspHI BsrGI BstEII BstYI BtgI BtgZI BtsI
DraI EaeI EarI Eco57MI EcoO109I FalI HindIII KpnI
MmeI MslI NcoI NdeI NsiI NspI PpuMI PspOMI
PstI SapI SfcI StyI TatI XmnI ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AgeI AhdI AlfI AloI
AlwNI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BglI BlpI BmgBI
BmtI BplI BpuEI BsaI BsaBI BsaWI BsgI BsiEI
BsiHKAI BsiWI BsmBI BspEI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstXI BstZ17I Bsu36I ClaI
CspCI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI HaeII Hin4I HincII HpaI
KasI MfeI MluI MscI MspA1I NaeI NarI NgoMIV
NheI NotI NruI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TstI
Tth111I XbaI XcmI XhoI XmaI