Restriction map for
pF1KB3956
(Linear) (Six-base) MAPSORT of: KIBB3956.seq Check: 9367 from: 1 to: 1152
KIBB3956.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 1152
Size: 66 1086
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 814 1152
Size: 814 338
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 736 768 1152
Size: 736 32 384
Fragments arranged by size:
736 384 32
AlwNI CAG_nnn'CTG
Cuts at: 0 766 1152
Size: 766 386
ApaI G_GGCC'C
Cuts at: 0 703 1152
Size: 703 449
AvaI C'yCGr_G
Cuts at: 0 740 1152
Size: 740 412
BanI G'GyrC_C
Cuts at: 0 864 1113 1152
Size: 864 249 39
Fragments arranged by size:
864 249 39
BanII G_rGCy'C
Cuts at: 0 703 749 1152
Size: 703 46 403
Fragments arranged by size:
703 403 46
BbsI GAAGACnn'nnnn_
Cuts at: 0 820 1152
Size: 820 332
BclI T'GATC_A
Cuts at: 0 980 1152
Size: 980 172
BglI GCCn_nnn'nGGC
Cuts at: 0 283 439 1152
Size: 283 156 713
Fragments arranged by size:
713 283 156
BglII A'GATC_T
Cuts at: 0 614 629 1152
Size: 614 15 523
Fragments arranged by size:
614 523 15
Bme1580I G_kGCm'C
Cuts at: 0 703 867 1118 1152
Size: 703 164 251 34
Fragments arranged by size:
703 251 164 34
BsaAI yAC'GTr
Cuts at: 0 342 375 948 1152
Size: 342 33 573 204
Fragments arranged by size:
573 342 204 33
BsaHI Gr'CG_yC
Cuts at: 0 1056 1152
Size: 1056 96
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 46 76 736 766 1152
Size: 46 30 660 30 386
Fragments arranged by size:
660 386 46 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 54 1152
Size: 54 1098
BseYI C'CCAG_C
Cuts at: 0 860 1064 1152
Size: 860 204 88
Fragments arranged by size:
860 204 88
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1033 1152
Size: 1033 119
BsiHKAI G_wGCw'C
Cuts at: 0 380 749 830 1152
Size: 380 369 81 322
Fragments arranged by size:
380 369 322 81
BsmI GAATG_Cn'
Cuts at: 0 836 1152
Size: 836 316
BsmBI CGTCTCn'nnnn_
Cuts at: 0 414 426 1152
Size: 414 12 726
Fragments arranged by size:
726 414 12
Bsp1286I G_dGCh'C
Cuts at: 0 380 703 749 830 867 1118 1152
Size: 380 323 46 81 37 251 34
Fragments arranged by size:
380 323 251 81 46 37 34
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 407 1152
Size: 407 745
BsrDI GCAATG_nn'
Cuts at: 0 170 272 443 548 1152
Size: 170 102 171 105 604
Fragments arranged by size:
604 171 170 105 102
BstEII G'GTnAC_C
Cuts at: 0 708 1152
Size: 708 444
BstYI r'GATC_y
Cuts at: 0 614 629 734 1152
Size: 614 15 105 418
Fragments arranged by size:
614 418 105 15
BtgI C'CryG_G
Cuts at: 0 487 1152
Size: 487 665
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 945 1152
Size: 945 207
BtsI GCAGTG_nn'
Cuts at: 0 935 1152
Size: 935 217
DraIII CAC_nnn'GTG
Cuts at: 0 992 1152
Size: 992 160
EaeI y'GGCC_r
Cuts at: 0 284 305 369 440 624 1152
Size: 284 21 64 71 184 528
Fragments arranged by size:
528 284 184 71 64 21
EarI CTCTTCn'nnn_
Cuts at: 0 679 1121 1152
Size: 679 442 31
Fragments arranged by size:
679 442 31
EcoICRI GAG'CTC
Cuts at: 0 747 1152
Size: 747 405
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 1152
Size: 66 1086
EcoO109I rG'GnC_Cy
Cuts at: 0 700 1152
Size: 700 452
EcoRV GAT'ATC
Cuts at: 0 460 1152
Size: 460 692
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 684 1152
Size: 684 468
FspI TGC'GCA
Cuts at: 0 253 403 1152
Size: 253 150 749
Fragments arranged by size:
749 253 150
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 46 78 483 515 1152
Size: 46 32 405 32 637
Fragments arranged by size:
637 405 46 32 32
HincII GTy'rAC
Cuts at: 0 393 1152
Size: 393 759
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 317 1152
Size: 317 835
MscI TGG'CCA
Cuts at: 0 286 307 371 442 626 1152
Size: 286 21 64 71 184 526
Fragments arranged by size:
526 286 184 71 64 21
MslI CAynn'nnrTG
Cuts at: 0 964 1152
Size: 964 188
MspA1I CmG'CkG
Cuts at: 0 1068 1152
Size: 1068 84
PasI CC'CwG_GG
Cuts at: 0 766 1152
Size: 766 386
PmlI CAC'GTG
Cuts at: 0 375 1152
Size: 375 777
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 736 768 1152
Size: 736 32 384
Fragments arranged by size:
736 384 32
PspOMI G'GGCC_C
Cuts at: 0 699 1152
Size: 699 453
SacI G_AGCT'C
Cuts at: 0 749 1152
Size: 749 403
ScaI AGT'ACT
Cuts at: 0 1027 1152
Size: 1027 125
SmaI CCC'GGG
Cuts at: 0 742 1152
Size: 742 410
StyI C'CwwG_G
Cuts at: 0 524 1152
Size: 524 628
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 48 1152
Size: 48 1104
TatI w'GTAC_w
Cuts at: 0 1025 1152
Size: 1025 127
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 435 1128 1152
Size: 435 693 24
Fragments arranged by size:
693 435 24
XmaI C'CCGG_G
Cuts at: 0 740 1152
Size: 740 412
XmnI GAAnn'nnTTC
Cuts at: 0 616 1027 1152
Size: 616 411 125
Fragments arranged by size:
616 411 125
Enzymes that do cut:
AcuI AlfI AloI AlwNI ApaI AvaI BanI BanII
BbsI BclI BglI BglII Bme1580I BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrDI BstEII BstYI BtgI BtgZI BtsI DraIII EaeI
EarI EcoICRI Eco57MI EcoO109I EcoRV FalI FspI Hin4I
HincII MmeI MscI MslI MspA1I PasI PmlI PpiI
PspOMI SacI ScaI SmaI StyI TaqII TatI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BfrBI
BlpI BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaBI BsaWI BsiEI BsiWI BspEI BspHI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I
Bsu36I ClaI CspCI DraI DrdI EagI EciI EcoNI
EcoRI FseI FspAI HaeII HindIII HpaI KasI KpnI
MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PflMI PfoI
PmeI PpuMI PshAI PsiI PspXI PsrI PstI PvuI
PvuII RsrII SacII SalI SanDI SapI SbfI SexAI
SfcI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI Tth111I XbaI
XhoI ZraI