Restriction map for
pF1KB3962
(Linear) (Six-base) MAPSORT of: KIBB3962.seq Check: 3261 from: 1 to: 1515
KIBB3962.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AccI GT'mk_AC
Cuts at: 0 427 1515
Size: 427 1088
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 1172 1406 1515
Size: 281 891 234 109
Fragments arranged by size:
891 281 234 109
AflIII A'CryG_T
Cuts at: 0 15 1515
Size: 15 1500
AleI CACnn'nnGTG
Cuts at: 0 760 1515
Size: 760 755
AlwNI CAG_nnn'CTG
Cuts at: 0 1411 1459 1515
Size: 1411 48 56
Fragments arranged by size:
1411 56 48
ApoI r'AATT_y
Cuts at: 0 4 21 160 283 1515
Size: 4 17 139 123 1232
Fragments arranged by size:
1232 139 123 17 4
BanI G'GyrC_C
Cuts at: 0 600 762 1515
Size: 600 162 753
Fragments arranged by size:
753 600 162
BbsI GAAGACnn'nnnn_
Cuts at: 0 1160 1322 1515
Size: 1160 162 193
Fragments arranged by size:
1160 193 162
BclI T'GATC_A
Cuts at: 0 198 1515
Size: 198 1317
BmrI ACTGGGnnnn_n'
Cuts at: 0 1488 1515
Size: 1488 27
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 845 968 1515
Size: 845 123 547
Fragments arranged by size:
845 547 123
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 525 564 1179 1515
Size: 525 39 615 336
Fragments arranged by size:
615 525 336 39
BsaAI yAC'GTr
Cuts at: 0 175 1515
Size: 175 1340
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 957 987 1515
Size: 957 30 528
Fragments arranged by size:
957 528 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 385 1515
Size: 385 1130
BseYI C'CCAG_C
Cuts at: 0 848 1515
Size: 848 667
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 452 1515
Size: 452 1063
BsiEI CG_ry'CG
Cuts at: 0 934 1515
Size: 934 581
BstAPI GCAn_nnn'nTGC
Cuts at: 0 466 1515
Size: 466 1049
BstYI r'GATC_y
Cuts at: 0 1047 1515
Size: 1047 468
Bsu36I CC'TnA_GG
Cuts at: 0 1506 1515
Size: 1506 9
BtgI C'CryG_G
Cuts at: 0 758 1515
Size: 758 757
EaeI y'GGCC_r
Cuts at: 0 96 1515
Size: 96 1419
EarI CTCTTCn'nnn_
Cuts at: 0 653 1026 1515
Size: 653 373 489
Fragments arranged by size:
653 489 373
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1456 1515
Size: 1456 59
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 845 968 1172 1406 1515
Size: 281 564 123 204 234 109
Fragments arranged by size:
564 281 234 204 123 109
EcoNI CCTnn'n_nnAGG
Cuts at: 0 612 632 1515
Size: 612 20 883
Fragments arranged by size:
883 612 20
EcoO109I rG'GnC_Cy
Cuts at: 0 42 787 1515
Size: 42 745 728
Fragments arranged by size:
745 728 42
EcoRI G'AATT_C
Cuts at: 0 21 283 1515
Size: 21 262 1232
Fragments arranged by size:
1232 262 21
HindIII A'AGCT_T
Cuts at: 0 293 1515
Size: 293 1222
MfeI C'AATT_G
Cuts at: 0 620 1515
Size: 620 895
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1006 1515
Size: 1006 509
MscI TGG'CCA
Cuts at: 0 98 1515
Size: 98 1417
MslI CAynn'nnrTG
Cuts at: 0 143 760 1324 1435 1515
Size: 143 617 564 111 80
Fragments arranged by size:
617 564 143 111 80
MspA1I CmG'CkG
Cuts at: 0 730 1179 1221 1515
Size: 730 449 42 294
Fragments arranged by size:
730 449 294 42
NcoI C'CATG_G
Cuts at: 0 758 1515
Size: 758 757
NspI r_CATG'y
Cuts at: 0 19 724 1515
Size: 19 705 791
Fragments arranged by size:
791 705 19
PciI A'CATG_T
Cuts at: 0 15 1515
Size: 15 1500
PfoI T'CCnGG_A
Cuts at: 0 774 1515
Size: 774 741
PpuMI rG'GwC_Cy
Cuts at: 0 42 787 1515
Size: 42 745 728
Fragments arranged by size:
745 728 42
PshAI GACnn'nnGTC
Cuts at: 0 936 1515
Size: 936 579
PstI C_TGCA'G
Cuts at: 0 735 1184 1515
Size: 735 449 331
Fragments arranged by size:
735 449 331
PvuII CAG'CTG
Cuts at: 0 730 1179 1221 1515
Size: 730 449 42 294
Fragments arranged by size:
730 449 294 42
SapI GCTCTTCn'nnn_
Cuts at: 0 1026 1515
Size: 1026 489
ScaI AGT'ACT
Cuts at: 0 585 1515
Size: 585 930
SfcI C'TryA_G
Cuts at: 0 731 965 1180 1412 1515
Size: 731 234 215 232 103
Fragments arranged by size:
731 234 232 215 103
SmlI C'TyrA_G
Cuts at: 0 540 579 1158 1515
Size: 540 39 579 357
Fragments arranged by size:
579 540 357 39
StyI C'CwwG_G
Cuts at: 0 758 1400 1515
Size: 758 642 115
Fragments arranged by size:
758 642 115
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 434 610 1356 1447 1515
Size: 434 176 746 91 68
Fragments arranged by size:
746 434 176 91 68
TatI w'GTAC_w
Cuts at: 0 79 583 807 1012 1450 1515
Size: 79 504 224 205 438 65
Fragments arranged by size:
504 438 224 205 79 65
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 42 74 1515
Size: 42 32 1441
Fragments arranged by size:
1441 42 32
Tth111I GACn'n_nGTC
Cuts at: 0 184 915 1515
Size: 184 731 600
Fragments arranged by size:
731 600 184
XbaI T'CTAG_A
Cuts at: 0 324 1515
Size: 324 1191
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1015 1515
Size: 1015 500
XmnI GAAnn'nnTTC
Cuts at: 0 283 1515
Size: 283 1232
Enzymes that do cut:
AccI AcuI AflIII AleI AlwNI ApoI BanI BbsI
BclI BmrI BpmI BpuEI BsaAI BsaXI BseRI BseYI
BsgI BsiEI BstAPI BstYI Bsu36I BtgI EaeI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI HindIII MfeI MmeI
MscI MslI MspA1I NcoI NspI PciI PfoI PpuMI
PshAI PstI PvuII SapI ScaI SfcI SmlI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanII BbeI BbvCI BcgI BciVI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmtI BplI
Bpu10I BsaI BsaBI BsaHI BsaWI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EcoICRI
EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII
HpaI KasI KpnI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PflMI PmeI
PmlI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI XhoI XmaI ZraI