Restriction map for
pF1KB3963
(Linear) (Six-base) MAPSORT of: KIBB3963.seq Check: 1370 from: 1 to: 2139
KIBB3963.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 948 2139
Size: 948 1191
AatII G_ACGT'C
Cuts at: 0 1361 2139
Size: 1361 778
AccI GT'mk_AC
Cuts at: 0 1637 2139
Size: 1637 502
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 147 1790 2073 2106 2139
Size: 147 1643 283 33 33
Fragments arranged by size:
1643 283 147 33 33
AflIII A'CryG_T
Cuts at: 0 890 2139
Size: 890 1249
AhdI GACnn_n'nnGTC
Cuts at: 0 1623 2139
Size: 1623 516
AleI CACnn'nnGTG
Cuts at: 0 787 931 1609 2139
Size: 787 144 678 530
Fragments arranged by size:
787 678 530 144
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1501 2139
Size: 1501 638
AlwNI CAG_nnn'CTG
Cuts at: 0 48 170 1768 2139
Size: 48 122 1598 371
Fragments arranged by size:
1598 371 122 48
ApaI G_GGCC'C
Cuts at: 0 1423 2082 2139
Size: 1423 659 57
Fragments arranged by size:
1423 659 57
ApoI r'AATT_y
Cuts at: 0 127 376 557 1084 1298 1855 2049 2139
Size: 127 249 181 527 214 557 194 90
Fragments arranged by size:
557 527 249 214 194 181 127 90
AvaI C'yCGr_G
Cuts at: 0 204 673 1219 2139
Size: 204 469 546 920
Fragments arranged by size:
920 546 469 204
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2020 2053 2139
Size: 2020 33 86
Fragments arranged by size:
2020 86 33
BanI G'GyrC_C
Cuts at: 0 684 781 2139
Size: 684 97 1358
Fragments arranged by size:
1358 684 97
BanII G_rGCy'C
Cuts at: 0 211 358 1423 2082 2139
Size: 211 147 1065 659 57
Fragments arranged by size:
1065 659 211 147 57
BbsI GAAGACnn'nnnn_
Cuts at: 0 1346 1419 2139
Size: 1346 73 720
Fragments arranged by size:
1346 720 73
BbvCI CC'TCA_GC
Cuts at: 0 2113 2139
Size: 2113 26
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 468 502 654 688 2139
Size: 468 34 152 34 1451
Fragments arranged by size:
1451 468 152 34 34
BclI T'GATC_A
Cuts at: 0 708 986 1071 1322 2139
Size: 708 278 85 251 817
Fragments arranged by size:
817 708 278 251 85
BglII A'GATC_T
Cuts at: 0 1898 2019 2139
Size: 1898 121 120
Fragments arranged by size:
1898 121 120
BlpI GC'TnA_GC
Cuts at: 0 2127 2139
Size: 2127 12
Bme1580I G_kGCm'C
Cuts at: 0 1423 1702 2082 2139
Size: 1423 279 380 57
Fragments arranged by size:
1423 380 279 57
BmgBI CAC'GTC
Cuts at: 0 759 1731 2139
Size: 759 972 408
Fragments arranged by size:
972 759 408
BmrI ACTGGGnnnn_n'
Cuts at: 0 1417 1712 2139
Size: 1417 295 427
Fragments arranged by size:
1417 427 295
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1485 2139
Size: 1485 654
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1744 2139
Size: 1744 395
Bpu10I CC'TnA_GC
Cuts at: 0 2113 2139
Size: 2113 26
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 983 2139
Size: 983 1156
BsaI GGTCTCn'nnnn_
Cuts at: 0 207 640 2139
Size: 207 433 1499
Fragments arranged by size:
1499 433 207
BsaBI GATnn'nnATC
Cuts at: 0 1054 1826 2139
Size: 1054 772 313
Fragments arranged by size:
1054 772 313
BsaHI Gr'CG_yC
Cuts at: 0 868 1358 2139
Size: 868 490 781
Fragments arranged by size:
868 781 490
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 908 938 1124 1154 1498 1528 2139
Size: 908 30 186 30 344 30 611
Fragments arranged by size:
908 611 344 186 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2101 2139
Size: 2101 38
BseYI C'CCAG_C
Cuts at: 0 822 1372 1557 2139
Size: 822 550 185 582
Fragments arranged by size:
822 582 550 185
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 2139
Size: 63 2076
BsiEI CG_ry'CG
Cuts at: 0 727 2139
Size: 727 1412
BsiHKAI G_wGCw'C
Cuts at: 0 2041 2139
Size: 2041 98
BsmI GAATG_Cn'
Cuts at: 0 1503 2139
Size: 1503 636
BsmBI CGTCTCn'nnnn_
Cuts at: 0 594 1365 2139
Size: 594 771 774
Fragments arranged by size:
774 771 594
Bsp1286I G_dGCh'C
Cuts at: 0 211 358 1423 1702 2041 2082 2139
Size: 211 147 1065 279 339 41 57
Fragments arranged by size:
1065 339 279 211 147 57 41
BspHI T'CATG_A
Cuts at: 0 1052 2139
Size: 1052 1087
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 948 1552 1976 2139
Size: 948 604 424 163
Fragments arranged by size:
948 604 424 163
BsrBI CCG'CTC
Cuts at: 0 18 2139
Size: 18 2121
BsrFI r'CCGG_y
Cuts at: 0 681 2139
Size: 681 1458
BsrGI T'GTAC_A
Cuts at: 0 1649 2139
Size: 1649 490
BssSI C'ACGA_G
Cuts at: 0 325 2139
Size: 325 1814
BstEII G'GTnAC_C
Cuts at: 0 770 1449 2139
Size: 770 679 690
Fragments arranged by size:
770 690 679
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1565 2139
Size: 1565 574
BstYI r'GATC_y
Cuts at: 0 1827 1845 1898 2019 2139
Size: 1827 18 53 121 120
Fragments arranged by size:
1827 121 120 53 18
BtgI C'CryG_G
Cuts at: 0 1502 2139
Size: 1502 637
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 245 2139
Size: 245 1894
BtsI GCAGTG_nn'
Cuts at: 0 272 1652 2139
Size: 272 1380 487
Fragments arranged by size:
1380 487 272
ClaI AT'CG_AT
Cuts at: 0 902 1058 2139
Size: 902 156 1081
Fragments arranged by size:
1081 902 156
DraI TTT'AAA
Cuts at: 0 375 2139
Size: 375 1764
EaeI y'GGCC_r
Cuts at: 0 753 1037 2139
Size: 753 284 1102
Fragments arranged by size:
1102 753 284
EarI CTCTTCn'nnn_
Cuts at: 0 1666 2125 2139
Size: 1666 459 14
Fragments arranged by size:
1666 459 14
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 147 1744 1790 2073 2106 2139
Size: 147 1597 46 283 33 33
Fragments arranged by size:
1597 283 147 46 33 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 40 911 2139
Size: 40 871 1228
Fragments arranged by size:
1228 871 40
EcoO109I rG'GnC_Cy
Cuts at: 0 742 1419 1464 1554 2078 2079 2139
Size: 742 677 45 90 524 1 60
Fragments arranged by size:
742 677 524 90 60 45 1
EcoRI G'AATT_C
Cuts at: 0 127 2049 2139
Size: 127 1922 90
Fragments arranged by size:
1922 127 90
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1285 2139
Size: 1285 854
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 127 159 605 637 865 897 908 940
Size: 127 32 446 32 228 32 11 32
Cuts at: 940 1124 1156 1498 1530 1609 1641 2139
Size: 184 32 342 32 79 32 498
Fragments arranged by size:
498 446 342 228 184 127 79 32
32 32 32 32 32 32 11
HincII GTy'rAC
Cuts at: 0 1413 1599 1638 2139
Size: 1413 186 39 501
Fragments arranged by size:
1413 501 186 39
MfeI C'AATT_G
Cuts at: 0 1670 2139
Size: 1670 469
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1002 2139
Size: 1002 1137
MslI CAynn'nnrTG
Cuts at: 0 787 790 931 1051 1447 1609 1978 2139
Size: 787 3 141 120 396 162 369 161
Fragments arranged by size:
787 396 369 162 161 141 120 3
MspA1I CmG'CkG
Cuts at: 0 124 1609 2139
Size: 124 1485 530
Fragments arranged by size:
1485 530 124
NspI r_CATG'y
Cuts at: 0 894 2139
Size: 894 1245
PciI A'CATG_T
Cuts at: 0 890 2139
Size: 890 1249
PflMI CCAn_nnn'nTGG
Cuts at: 0 762 2139
Size: 762 1377
PfoI T'CCnGG_A
Cuts at: 0 84 1403 2139
Size: 84 1319 736
Fragments arranged by size:
1319 736 84
PpuMI rG'GwC_Cy
Cuts at: 0 1464 1554 2139
Size: 1464 90 585
Fragments arranged by size:
1464 585 90
PspOMI G'GGCC_C
Cuts at: 0 1419 2078 2139
Size: 1419 659 61
Fragments arranged by size:
1419 659 61
PstI C_TGCA'G
Cuts at: 0 66 267 1523 2047 2139
Size: 66 201 1256 524 92
Fragments arranged by size:
1256 524 201 92 66
PvuII CAG'CTG
Cuts at: 0 124 1609 2139
Size: 124 1485 530
Fragments arranged by size:
1485 530 124
SalI G'TCGA_C
Cuts at: 0 1636 2139
Size: 1636 503
SbfI CC_TGCA'GG
Cuts at: 0 2047 2139
Size: 2047 92
SfcI C'TryA_G
Cuts at: 0 62 263 1519 2043 2139
Size: 62 201 1256 524 96
Fragments arranged by size:
1256 524 201 96 62
SmaI CCC'GGG
Cuts at: 0 1221 2139
Size: 1221 918
SmlI C'TyrA_G
Cuts at: 0 204 673 998 2139
Size: 204 469 325 1141
Fragments arranged by size:
1141 469 325 204
SspI AAT'ATT
Cuts at: 0 1575 2139
Size: 1575 564
StyI C'CwwG_G
Cuts at: 0 605 2139
Size: 605 1534
SwaI ATTT'AAAT
Cuts at: 0 375 2139
Size: 375 1764
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 741 789 968 2016 2139
Size: 741 48 179 1048 123
Fragments arranged by size:
1048 741 179 123 48
TatI w'GTAC_w
Cuts at: 0 1649 2139
Size: 1649 490
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 521 553 2139
Size: 521 32 1586
Fragments arranged by size:
1586 521 32
Tth111I GACn'n_nGTC
Cuts at: 0 922 2139
Size: 922 1217
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1611 2139
Size: 1611 528
XhoI C'TCGA_G
Cuts at: 0 204 673 2139
Size: 204 469 1466
Fragments arranged by size:
1466 469 204
XmaI C'CCGG_G
Cuts at: 0 1219 2139
Size: 1219 920
XmnI GAAnn'nnTTC
Cuts at: 0 103 2139
Size: 103 2036
ZraI GAC'GTC
Cuts at: 0 1359 2139
Size: 1359 780
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AhdI AleI AlfI
AlwNI ApaI ApoI AvaI BaeI BanI BanII BbsI
BbvCI BcgI BclI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrFI BsrGI BssSI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI DraI EaeI EarI
Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I HincII MfeI
MmeI MslI MspA1I NspI PciI PflMI PfoI PpuMI
PspOMI PstI PvuII SalI SbfI SfcI SmaI SmlI
SspI StyI SwaI TaqII TatI TstI Tth111I XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AloI ApaLI AscI
AseI AsiSI AvrII BamHI BbeI BciVI BfrBI BglI
BmtI BsaAI BsaWI BsiWI BspEI BsrDI BssHII BstAPI
BstBI BstZ17I Bsu36I CspCI DraIII DrdI EagI EciI
EcoICRI EcoRV FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MluI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SanDI SapI ScaI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI StuI XbaI