(Linear) (Six-base) MAPSORT of: KIBB3964.seq Check: 8226 from: 1 to: 567 KIBB3964.orf With 182 enzymes: SgfI * April 24, 2009 13:02 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 22 567 Size: 22 545 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 478 511 567 Size: 478 33 56 Fragments arranged by size: 478 56 33 BbvCI CC'TCA_GC Cuts at: 0 432 567 Size: 432 135 BciVI GTATCCnnnnn_n' Cuts at: 0 162 567 Size: 162 405 Bpu10I CC'TnA_GC Cuts at: 0 432 567 Size: 432 135 BsaI GGTCTCn'nnnn_ Cuts at: 0 197 567 Size: 197 370 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 22 567 Size: 22 545 BtsI GCAGTG_nn' Cuts at: 0 525 567 Size: 525 42 EaeI y'GGCC_r Cuts at: 0 398 567 Size: 398 169 EarI CTCTTCn'nnn_ Cuts at: 0 179 567 Size: 179 388 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 6 137 567 Size: 6 131 430 Fragments arranged by size: 430 131 6 MscI TGG'CCA Cuts at: 0 400 567 Size: 400 167 MspA1I CmG'CkG Cuts at: 0 175 567 Size: 175 392 PflMI CCAn_nnn'nTGG Cuts at: 0 396 567 Size: 396 171 PvuII CAG'CTG Cuts at: 0 175 567 Size: 175 392 SfcI C'TryA_G Cuts at: 0 285 567 Size: 285 282 TatI w'GTAC_w Cuts at: 0 8 188 567 Size: 8 180 379 Fragments arranged by size: 379 180 8 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 44 76 567 Size: 44 32 491 Fragments arranged by size: 491 44 32 Enzymes that do cut: AarI BaeI BbvCI BciVI Bpu10I BsaI BspMI BtsI EaeI EarI MmeI MscI MspA1I PflMI PvuII SfcI TatI TstI Enzymes that do not cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TaqII Tth111I XbaI XcmI XhoI XmaI XmnI ZraI