Restriction map for
pF1KB3965
(Linear) (Six-base) MAPSORT of: KIBB3965.seq Check: 865 from: 1 to: 2292
KIBB3965.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 127 2292
Size: 127 2165
AatII G_ACGT'C
Cuts at: 0 496 2292
Size: 496 1796
AccI GT'mk_AC
Cuts at: 0 671 1418 1528 2292
Size: 671 747 110 764
Fragments arranged by size:
764 747 671 110
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 190 554 565 766 2292
Size: 93 97 364 11 201 1526
Fragments arranged by size:
1526 364 201 97 93 11
AfeI AGC'GCT
Cuts at: 0 1555 2292
Size: 1555 737
AflII C'TTAA_G
Cuts at: 0 778 2292
Size: 778 1514
AflIII A'CryG_T
Cuts at: 0 695 2292
Size: 695 1597
AleI CACnn'nnGTG
Cuts at: 0 848 1613 2292
Size: 848 765 679
Fragments arranged by size:
848 765 679
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1398 1631 2292
Size: 1398 233 661
Fragments arranged by size:
1398 661 233
AlwNI CAG_nnn'CTG
Cuts at: 0 168 273 1474 1703 2292
Size: 168 105 1201 229 589
Fragments arranged by size:
1201 589 229 168 105
ApoI r'AATT_y
Cuts at: 0 320 2292
Size: 320 1972
BanII G_rGCy'C
Cuts at: 0 374 1109 2292
Size: 374 735 1183
Fragments arranged by size:
1183 735 374
BbsI GAAGACnn'nnnn_
Cuts at: 0 1053 1187 1232 2292
Size: 1053 134 45 1060
Fragments arranged by size:
1060 1053 134 45
BciVI GTATCCnnnnn_n'
Cuts at: 0 1026 2292
Size: 1026 1266
BfrBI ATG'CAT
Cuts at: 0 211 658 1907 2292
Size: 211 447 1249 385
Fragments arranged by size:
1249 447 385 211
BglII A'GATC_T
Cuts at: 0 1808 2292
Size: 1808 484
BmgBI CAC'GTC
Cuts at: 0 696 2292
Size: 696 1596
BmrI ACTGGGnnnn_n'
Cuts at: 0 149 2292
Size: 149 2143
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2150 2292
Size: 2150 142
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 757 1570 2292
Size: 757 813 722
Fragments arranged by size:
813 757 722
Bpu10I CC'TnA_GC
Cuts at: 0 2108 2292
Size: 2108 184
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 819 907 2292
Size: 819 88 1385
Fragments arranged by size:
1385 819 88
BsaI GGTCTCn'nnnn_
Cuts at: 0 1717 2197 2292
Size: 1717 480 95
Fragments arranged by size:
1717 480 95
BsaAI yAC'GTr
Cuts at: 0 246 2292
Size: 246 2046
BsaBI GATnn'nnATC
Cuts at: 0 1813 2292
Size: 1813 479
BsaHI Gr'CG_yC
Cuts at: 0 493 2292
Size: 493 1799
BsaWI w'CCGG_w
Cuts at: 0 1589 2292
Size: 1589 703
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1543 1573 2292
Size: 1543 30 719
Fragments arranged by size:
1543 719 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 115 680 1306 1393 2292
Size: 115 565 626 87 899
Fragments arranged by size:
899 626 565 115 87
BseYI C'CCAG_C
Cuts at: 0 142 1634 2292
Size: 142 1492 658
Fragments arranged by size:
1492 658 142
BsiHKAI G_wGCw'C
Cuts at: 0 374 743 1109 2292
Size: 374 369 366 1183
Fragments arranged by size:
1183 374 369 366
BsmI GAATG_Cn'
Cuts at: 0 513 951 2292
Size: 513 438 1341
Fragments arranged by size:
1341 513 438
BsmBI CGTCTCn'nnnn_
Cuts at: 0 80 2292
Size: 80 2212
Bsp1286I G_dGCh'C
Cuts at: 0 374 743 1109 2292
Size: 374 369 366 1183
Fragments arranged by size:
1183 374 369 366
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 50 127 1406 2292
Size: 50 77 1279 886
Fragments arranged by size:
1279 886 77 50
BsrDI GCAATG_nn'
Cuts at: 0 534 1299 2292
Size: 534 765 993
Fragments arranged by size:
993 765 534
BsrFI r'CCGG_y
Cuts at: 0 6 2292
Size: 6 2286
BsrGI T'GTAC_A
Cuts at: 0 551 2292
Size: 551 1741
BstAPI GCAn_nnn'nTGC
Cuts at: 0 27 924 2292
Size: 27 897 1368
Fragments arranged by size:
1368 897 27
BstEII G'GTnAC_C
Cuts at: 0 2169 2292
Size: 2169 123
BstXI CCAn_nnnn'nTGG
Cuts at: 0 54 735 1584 1875 2292
Size: 54 681 849 291 417
Fragments arranged by size:
849 681 417 291 54
BstYI r'GATC_y
Cuts at: 0 1595 1808 2292
Size: 1595 213 484
Fragments arranged by size:
1595 484 213
BstZ17I GTA'TAC
Cuts at: 0 672 1529 2292
Size: 672 857 763
Fragments arranged by size:
857 763 672
Bsu36I CC'TnA_GG
Cuts at: 0 1603 2292
Size: 1603 689
BtgI C'CryG_G
Cuts at: 0 1712 2292
Size: 1712 580
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1392 2292
Size: 1392 900
BtsI GCAGTG_nn'
Cuts at: 0 713 843 2292
Size: 713 130 1449
Fragments arranged by size:
1449 713 130
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 779 814 2292
Size: 779 35 1478
Fragments arranged by size:
1478 779 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 112 2292
Size: 112 2180
EaeI y'GGCC_r
Cuts at: 0 1098 2292
Size: 1098 1194
EarI CTCTTCn'nnn_
Cuts at: 0 974 1118 1999 2264 2292
Size: 974 144 881 265 28
Fragments arranged by size:
974 881 265 144 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 25 74 1588 2292
Size: 25 49 1514 704
Fragments arranged by size:
1514 704 49 25
EcoICRI GAG'CTC
Cuts at: 0 372 1107 2292
Size: 372 735 1185
Fragments arranged by size:
1185 735 372
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 190 554 565 757 766 1570 2292
Size: 93 97 364 11 192 9 804 722
Fragments arranged by size:
804 722 364 192 97 93 11 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 967 2055 2292
Size: 967 1088 237
Fragments arranged by size:
1088 967 237
EcoO109I rG'GnC_Cy
Cuts at: 0 51 1471 2292
Size: 51 1420 821
Fragments arranged by size:
1420 821 51
HaeII r_GCGC'y
Cuts at: 0 1557 2292
Size: 1557 735
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2175 2207 2292
Size: 2175 32 85
Fragments arranged by size:
2175 85 32
HindIII A'AGCT_T
Cuts at: 0 172 684 1136 2292
Size: 172 512 452 1156
Fragments arranged by size:
1156 512 452 172
MscI TGG'CCA
Cuts at: 0 1100 2292
Size: 1100 1192
MslI CAynn'nnrTG
Cuts at: 0 848 1390 1613 1906 2292
Size: 848 542 223 293 386
Fragments arranged by size:
848 542 386 293 223
MspA1I CmG'CkG
Cuts at: 0 24 273 2292
Size: 24 249 2019
Fragments arranged by size:
2019 249 24
NaeI GCC'GGC
Cuts at: 0 8 2292
Size: 8 2284
NcoI C'CATG_G
Cuts at: 0 1712 2292
Size: 1712 580
NdeI CA'TA_TG
Cuts at: 0 1165 1903 2292
Size: 1165 738 389
Fragments arranged by size:
1165 738 389
NgoMIV G'CCGG_C
Cuts at: 0 6 2292
Size: 6 2286
NsiI A_TGCA'T
Cuts at: 0 213 660 1909 2292
Size: 213 447 1249 383
Fragments arranged by size:
1249 447 383 213
NspI r_CATG'y
Cuts at: 0 1616 2292
Size: 1616 676
PasI CC'CwG_GG
Cuts at: 0 47 1474 1843 2292
Size: 47 1427 369 449
Fragments arranged by size:
1427 449 369 47
PflMI CCAn_nnn'nTGG
Cuts at: 0 1460 2202 2292
Size: 1460 742 90
Fragments arranged by size:
1460 742 90
PfoI T'CCnGG_A
Cuts at: 0 1028 2292
Size: 1028 1264
PpuMI rG'GwC_Cy
Cuts at: 0 51 2292
Size: 51 2241
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 328 360 2292
Size: 328 32 1932
Fragments arranged by size:
1932 328 32
PstI C_TGCA'G
Cuts at: 0 23 269 2292
Size: 23 246 2023
Fragments arranged by size:
2023 246 23
PvuII CAG'CTG
Cuts at: 0 24 273 2292
Size: 24 249 2019
Fragments arranged by size:
2019 249 24
SacI G_AGCT'C
Cuts at: 0 374 1109 2292
Size: 374 735 1183
Fragments arranged by size:
1183 735 374
ScaI AGT'ACT
Cuts at: 0 1441 2292
Size: 1441 851
SfcI C'TryA_G
Cuts at: 0 19 251 265 1415 2292
Size: 19 232 14 1150 877
Fragments arranged by size:
1150 877 232 19 14
SmlI C'TyrA_G
Cuts at: 0 778 798 886 2292
Size: 778 20 88 1406
Fragments arranged by size:
1406 778 88 20
SnaBI TAC'GTA
Cuts at: 0 246 2292
Size: 246 2046
SspI AAT'ATT
Cuts at: 0 231 2292
Size: 231 2061
StuI AGG'CCT
Cuts at: 0 734 2050 2292
Size: 734 1316 242
Fragments arranged by size:
1316 734 242
StyI C'CwwG_G
Cuts at: 0 390 576 1712 2045 2292
Size: 390 186 1136 333 247
Fragments arranged by size:
1136 390 333 247 186
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 979 1466 2292
Size: 979 487 826
Fragments arranged by size:
979 826 487
TatI w'GTAC_w
Cuts at: 0 551 1439 1835 2292
Size: 551 888 396 457
Fragments arranged by size:
888 551 457 396
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 705 737 2292
Size: 705 32 1555
Fragments arranged by size:
1555 705 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1983 2292
Size: 1983 309
ZraI GAC'GTC
Cuts at: 0 494 2292
Size: 494 1798
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflII AflIII AleI
AlfI AlwNI ApoI BanII BbsI BciVI BfrBI BglII
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI BsmI
BsmBI Bsp1286I BspMI BsrDI BsrFI BsrGI BstAPI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
HaeII Hin4I HindIII MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NsiI NspI PasI PflMI PfoI PpuMI
PsrI PstI PvuII SacI ScaI SfcI SmlI SnaBI
SspI StuI StyI TaqII TatI TstI XcmI ZraI
Enzymes that do not cut:
Acc65I AclI AgeI AhdI AloI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BbvCI BcgI BclI BglI BlpI Bme1580I BmtI BsgI
BsiEI BsiWI BspEI BspHI BsrBI BssHII BssSI BstBI
ClaI DraI DraIII EagI EcoRI EcoRV FalI FseI
FspI FspAI HincII HpaI KasI KpnI MfeI MluI
MmeI NarI NheI NotI NruI PacI PciI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PvuI RsrII
SacII SalI SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SpeI SphI SrfI SwaI Tth111I
XbaI XhoI XmaI XmnI