Restriction map for
pF1KB3975
(Linear) (Six-base) MAPSORT of: KIBB3975.seq Check: 6566 from: 1 to: 1887
KIBB3975.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1378 1887
Size: 1378 509
AccI GT'mk_AC
Cuts at: 0 185 956 1887
Size: 185 771 931
Fragments arranged by size:
931 771 185
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 547 921 1887
Size: 547 374 966
Fragments arranged by size:
966 547 374
AfeI AGC'GCT
Cuts at: 0 316 328 1887
Size: 316 12 1559
Fragments arranged by size:
1559 316 12
AflIII A'CryG_T
Cuts at: 0 884 1887
Size: 884 1003
AhdI GACnn_n'nnGTC
Cuts at: 0 888 1793 1887
Size: 888 905 94
Fragments arranged by size:
905 888 94
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 688 1778 1887
Size: 688 1090 109
Fragments arranged by size:
1090 688 109
AlwNI CAG_nnn'CTG
Cuts at: 0 788 1218 1536 1744 1887
Size: 788 430 318 208 143
Fragments arranged by size:
788 430 318 208 143
ApaI G_GGCC'C
Cuts at: 0 47 213 1108 1157 1327 1477 1484 1620
Size: 47 166 895 49 170 150 7 136
Cuts at: 1620 1887
Size: 267
Fragments arranged by size:
895 267 170 166 150 136 49 47
7
AvaI C'yCGr_G
Cuts at: 0 1108 1156 1887
Size: 1108 48 731
Fragments arranged by size:
1108 731 48
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1632 1665 1887
Size: 1632 33 222
Fragments arranged by size:
1632 222 33
BanI G'GyrC_C
Cuts at: 0 219 1017 1887
Size: 219 798 870
Fragments arranged by size:
870 798 219
BanII G_rGCy'C
Cuts at: 0 47 213 698 932 1108 1157 1247 1275
Size: 47 166 485 234 176 49 90 28
Cuts at: 1275 1327 1477 1484 1620 1887
Size: 52 150 7 136 267
Fragments arranged by size:
485 267 234 176 166 150 136 90
52 49 47 28 7
BbeI G_GCGC'C
Cuts at: 0 223 1021 1887
Size: 223 798 866
Fragments arranged by size:
866 798 223
BbvCI CC'TCA_GC
Cuts at: 0 123 1767 1887
Size: 123 1644 120
Fragments arranged by size:
1644 123 120
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1865 1887
Size: 1865 22
BclI T'GATC_A
Cuts at: 0 387 1887
Size: 387 1500
BglI GCCn_nnn'nGGC
Cuts at: 0 93 217 797 1309 1331 1539 1706 1887
Size: 93 124 580 512 22 208 167 181
Fragments arranged by size:
580 512 208 181 167 124 93 22
BlpI GC'TnA_GC
Cuts at: 0 697 1887
Size: 697 1190
Bme1580I G_kGCm'C
Cuts at: 0 47 213 862 1108 1157 1327 1477 1484
Size: 47 166 649 246 49 170 150 7
Cuts at: 1484 1565 1620 1887
Size: 81 55 267
Fragments arranged by size:
649 267 246 170 166 150 81 55
49 47 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 1614 1887
Size: 1614 273
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 949 1887
Size: 949 938
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 496 1255 1386 1401 1413 1430 1811
Size: 343 153 759 131 15 12 17 381
Cuts at: 1811 1887
Size: 76
Fragments arranged by size:
759 381 343 153 131 76 17 15
12
Bpu10I CC'TnA_GC
Cuts at: 0 123 692 1767 1887
Size: 123 569 1075 120
Fragments arranged by size:
1075 569 123 120
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 703 1887
Size: 703 1184
BsaHI Gr'CG_yC
Cuts at: 0 220 623 1018 1887
Size: 220 403 395 869
Fragments arranged by size:
869 403 395 220
BsaWI w'CCGG_w
Cuts at: 0 545 922 1439 1775 1887
Size: 545 377 517 336 112
Fragments arranged by size:
545 517 377 336 112
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 493 523 1887
Size: 493 30 1364
Fragments arranged by size:
1364 493 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1416 1887
Size: 1416 471
BseYI C'CCAG_C
Cuts at: 0 212 984 1134 1529 1631 1737 1751 1887
Size: 212 772 150 395 102 106 14 136
Fragments arranged by size:
772 395 212 150 136 106 102 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 648 1162 1887
Size: 648 514 725
Fragments arranged by size:
725 648 514
BsiEI CG_ry'CG
Cuts at: 0 1001 1304 1887
Size: 1001 303 583
Fragments arranged by size:
1001 583 303
BsiHKAI G_wGCw'C
Cuts at: 0 56 698 854 1873 1887
Size: 56 642 156 1019 14
Fragments arranged by size:
1019 642 156 56 14
BsmBI CGTCTCn'nnnn_
Cuts at: 0 630 1887
Size: 630 1257
Bsp1286I G_dGCh'C
Cuts at: 0 47 56 213 698 854 862 932 1108
Size: 47 9 157 485 156 8 70 176
Cuts at: 1108 1157 1247 1275 1327 1477 1484 1565 1620
Size: 49 90 28 52 150 7 81 55
Cuts at: 1620 1873 1887
Size: 253 14
Fragments arranged by size:
485 253 176 157 156 150 90 81
70 55 52 49 47 28 14 9
8 7
BspEI T'CCGG_A
Cuts at: 0 545 1775 1887
Size: 545 1230 112
Fragments arranged by size:
1230 545 112
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 51 1378 1887
Size: 51 1327 509
Fragments arranged by size:
1327 509 51
BsrBI CCG'CTC
Cuts at: 0 839 1887
Size: 839 1048
BsrFI r'CCGG_y
Cuts at: 0 996 1163 1887
Size: 996 167 724
Fragments arranged by size:
996 724 167
BstAPI GCAn_nnn'nTGC
Cuts at: 0 66 788 1131 1887
Size: 66 722 343 756
Fragments arranged by size:
756 722 343 66
BstYI r'GATC_y
Cuts at: 0 806 1887
Size: 806 1081
BstZ17I GTA'TAC
Cuts at: 0 186 1887
Size: 186 1701
Bsu36I CC'TnA_GG
Cuts at: 0 845 1887
Size: 845 1042
BtgI C'CryG_G
Cuts at: 0 18 267 618 1854 1887
Size: 18 249 351 1236 33
Fragments arranged by size:
1236 351 249 33 18
BtsI GCAGTG_nn'
Cuts at: 0 312 670 708 1344 1600 1887
Size: 312 358 38 636 256 287
Fragments arranged by size:
636 358 312 287 256 38
EaeI y'GGCC_r
Cuts at: 0 2 366 410 683 798 875 998 1301
Size: 2 364 44 273 115 77 123 303
Cuts at: 1301 1887
Size: 586
Fragments arranged by size:
586 364 303 273 123 115 77 44
2
EagI C'GGCC_G
Cuts at: 0 998 1301 1887
Size: 998 303 586
Fragments arranged by size:
998 586 303
EarI CTCTTCn'nnn_
Cuts at: 0 940 1383 1887
Size: 940 443 504
Fragments arranged by size:
940 504 443
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1282 1887
Size: 1282 605
EcoICRI GAG'CTC
Cuts at: 0 696 1887
Size: 696 1191
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 496 547 921 1255 1386 1401 1413
Size: 343 153 51 374 334 131 15 12
Cuts at: 1413 1430 1811 1887
Size: 17 381 76
Fragments arranged by size:
381 374 343 334 153 131 76 51
17 15 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 448 1887
Size: 448 1439
EcoO109I rG'GnC_Cy
Cuts at: 0 43 44 258 1104 1105 1215 1473 1474
Size: 43 1 214 846 1 110 258 1
Cuts at: 1474 1481 1518 1616 1837 1887
Size: 7 37 98 221 50
Fragments arranged by size:
846 258 221 214 110 98 50 43
37 7 1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 552 1887
Size: 552 1335
FspI TGC'GCA
Cuts at: 0 304 604 1228 1887
Size: 304 300 624 659
Fragments arranged by size:
659 624 304 300
HaeII r_GCGC'y
Cuts at: 0 223 318 330 761 1021 1744 1887
Size: 223 95 12 431 260 723 143
Fragments arranged by size:
723 431 260 223 143 95 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 106 138 562 594 874 906 1779 1811
Size: 106 32 424 32 280 32 873 32
Cuts at: 1811 1887
Size: 76
Fragments arranged by size:
873 424 280 106 76 32 32 32
32
HincII GTy'rAC
Cuts at: 0 957 1887
Size: 957 930
KasI G'GCGC_C
Cuts at: 0 219 1017 1887
Size: 219 798 870
Fragments arranged by size:
870 798 219
MscI TGG'CCA
Cuts at: 0 368 412 685 800 877 1887
Size: 368 44 273 115 77 1010
Fragments arranged by size:
1010 368 273 115 77 44
MslI CAynn'nnrTG
Cuts at: 0 176 272 502 883 907 1887
Size: 176 96 230 381 24 980
Fragments arranged by size:
980 381 230 176 96 24
MspA1I CmG'CkG
Cuts at: 0 132 620 1536 1887
Size: 132 488 916 351
Fragments arranged by size:
916 488 351 132
NaeI GCC'GGC
Cuts at: 0 1165 1887
Size: 1165 722
NarI GG'CG_CC
Cuts at: 0 220 1018 1887
Size: 220 798 869
Fragments arranged by size:
869 798 220
NcoI C'CATG_G
Cuts at: 0 18 267 1887
Size: 18 249 1620
Fragments arranged by size:
1620 249 18
NgoMIV G'CCGG_C
Cuts at: 0 1163 1887
Size: 1163 724
NspI r_CATG'y
Cuts at: 0 888 1233 1887
Size: 888 345 654
Fragments arranged by size:
888 654 345
PasI CC'CwG_GG
Cuts at: 0 40 894 1266 1477 1544 1887
Size: 40 854 372 211 67 343
Fragments arranged by size:
854 372 343 211 67 40
PciI A'CATG_T
Cuts at: 0 884 1887
Size: 884 1003
PflMI CCAn_nnn'nTGG
Cuts at: 0 1218 1887
Size: 1218 669
PfoI T'CCnGG_A
Cuts at: 0 110 1211 1887
Size: 110 1101 676
Fragments arranged by size:
1101 676 110
PpuMI rG'GwC_Cy
Cuts at: 0 258 1215 1518 1887
Size: 258 957 303 369
Fragments arranged by size:
957 369 303 258
PspOMI G'GGCC_C
Cuts at: 0 43 209 1104 1153 1323 1473 1480 1616
Size: 43 166 895 49 170 150 7 136
Cuts at: 1616 1887
Size: 271
Fragments arranged by size:
895 271 170 166 150 136 49 43
7
PstI C_TGCA'G
Cuts at: 0 1127 1280 1887
Size: 1127 153 607
Fragments arranged by size:
1127 607 153
SacI G_AGCT'C
Cuts at: 0 698 1887
Size: 698 1189
SacII CC_GC'GG
Cuts at: 0 621 1887
Size: 621 1266
SalI G'TCGA_C
Cuts at: 0 955 1887
Size: 955 932
ScaI AGT'ACT
Cuts at: 0 511 1036 1887
Size: 511 525 851
Fragments arranged by size:
851 525 511
SexAI A'CCwGG_T
Cuts at: 0 1217 1887
Size: 1217 670
SfcI C'TryA_G
Cuts at: 0 1123 1276 1452 1887
Size: 1123 153 176 435
Fragments arranged by size:
1123 435 176 153
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1309 1887
Size: 1309 578
SfoI GGC'GCC
Cuts at: 0 221 1019 1887
Size: 221 798 868
Fragments arranged by size:
868 798 221
SmaI CCC'GGG
Cuts at: 0 1110 1158 1887
Size: 1110 48 729
Fragments arranged by size:
1110 729 48
SmlI C'TyrA_G
Cuts at: 0 718 1887
Size: 718 1169
SphI G_CATG'C
Cuts at: 0 1233 1887
Size: 1233 654
StyI C'CwwG_G
Cuts at: 0 18 267 1022 1644 1887
Size: 18 249 755 622 243
Fragments arranged by size:
755 622 249 243 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 625 1751 1887
Size: 625 1126 136
Fragments arranged by size:
1126 625 136
TatI w'GTAC_w
Cuts at: 0 509 519 1034 1887
Size: 509 10 515 853
Fragments arranged by size:
853 515 509 10
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 768 1887
Size: 768 1119
XmaI C'CCGG_G
Cuts at: 0 1108 1156 1887
Size: 1108 48 731
Fragments arranged by size:
1108 731 48
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AhdI AlfI AlwNI
ApaI AvaI BaeI BanI BanII BbeI BbvCI BcgI
BclI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BstAPI
BstYI BstZ17I Bsu36I BtgI BtsI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII
Hin4I HincII KasI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PpuMI
PspOMI PstI SacI SacII SalI ScaI SexAI SfcI
SfiI SfoI SmaI SmlI SphI StyI TaqII TatI
XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AleI AloI ApaLI
ApoI AscI AseI AsiSI AvrII BamHI BbsI BciVI
BfrBI BglII BmgBI BmtI BsaI BsaAI BsaBI BsiWI
BsmI BspHI BsrDI BsrGI BssHII BssSI BstBI BstEII
BstXI BtgZI ClaI CspCI DraI DraIII DrdI EcoRI
EcoRV FseI FspAI HindIII HpaI KpnI MfeI MluI
MmeI NdeI NheI NotI NruI NsiI PacI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SanDI SapI SbfI SgfI SgrAI SnaBI SpeI
SrfI SspI StuI SwaI TstI Tth111I XbaI XhoI
XmnI ZraI