(Linear) (Six-base) MAPSORT of: KIBB3981.seq  Check: 3212  from: 1  to: 546



KIBB3981.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121     137     546 
   Size:        121      16     409

  Fragments arranged by size: 

                 409     121      16
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     479     511     546 
   Size:        479      32      35

  Fragments arranged by size: 

                 479      35      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     403     546 
   Size:        403     143
 
ApaLI G'TGCA_C

Cuts at:      0      35     546 
   Size:         35     511
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     125     546 
   Size:        125     421
 
BlpI GC'TnA_GC

Cuts at:      0     474     546 
   Size:        474      72
 
Bme1580I G_kGCm'C

Cuts at:      0      39     546 
   Size:         39     507
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     522     546 
   Size:        522      24
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     352     546 
   Size:        352     194
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     479     509     546 
   Size:        479      30      37

  Fragments arranged by size: 

                 479      37      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     302     546 
   Size:        302     244
 
BsiEI CG_ry'CG

Cuts at:      0     450     546 
   Size:        450      96
 
BsiHKAI G_wGCw'C

Cuts at:      0      39     365     546 
   Size:         39     326     181

  Fragments arranged by size: 

                 326     181      39
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     298     546 
   Size:        298     248
 
Bsp1286I G_dGCh'C

Cuts at:      0      39     365     546 
   Size:         39     326     181

  Fragments arranged by size: 

                 326     181      39
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     440     546 
   Size:        440     106
 
BsrGI T'GTAC_A

Cuts at:      0     165     546 
   Size:        165     381
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     387     403     546 
   Size:        387      16     143

  Fragments arranged by size: 

                 387     143      16
 
BstYI r'GATC_y

Cuts at:      0     461     546 
   Size:        461      85
 
DraI TTT'AAA

Cuts at:      0     183     546 
   Size:        183     363
 
EarI CTCTTCn'nnn_

Cuts at:      0     101     296     546 
   Size:        101     195     250

  Fragments arranged by size: 

                 250     195     101
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121     137     352     546 
   Size:        121      16     215     194

  Fragments arranged by size: 

                 215     194     121      16
 
FspI TGC'GCA

Cuts at:      0      22     546 
   Size:         22     524
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     479     511     546 
   Size:        479      32      35

  Fragments arranged by size: 

                 479      35      32
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     464     546 
   Size:        464      82
 
TatI w'GTAC_w

Cuts at:      0     165     546 
   Size:        165     381
 
Tth111I GACn'n_nGTC

Cuts at:      0     451     546 
   Size:        451      95
 

 Enzymes that do cut:

     AcuI     AloI    AlwNI    ApaLI     BbsI     BlpI Bme1580I     BmrI
     BpmI    BsaXI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI
    BsrGI   BstAPI    BstYI     DraI     EarI  Eco57MI     FspI     PpiI
    TaqII     TatI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     ApaI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII    BmgBI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BseYI    BsiWI     BsmI    BspEI    BspHI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI     TstI     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI