(Linear) (Six-base) MAPSORT of: KIBB3982.seq Check: 5784 from: 1 to: 357
KIBB3982.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
ApoI r'AATT_y
Cuts at: 0 56 248 357
Size: 56 192 109
Fragments arranged by size:
192 109 56
BciVI GTATCCnnnnn_n'
Cuts at: 0 6 357
Size: 6 351
BmgBI CAC'GTC
Cuts at: 0 227 357
Size: 227 130
BsaWI w'CCGG_w
Cuts at: 0 16 357
Size: 16 341
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 317 347 357
Size: 317 30 10
Fragments arranged by size:
317 30 10
BsmI GAATG_Cn'
Cuts at: 0 170 357
Size: 170 187
BsrDI GCAATG_nn'
Cuts at: 0 344 357
Size: 344 13
BstBI TT'CG_AA
Cuts at: 0 60 162 357
Size: 60 102 195
Fragments arranged by size:
195 102 60
BtgI C'CryG_G
Cuts at: 0 29 357
Size: 29 328
EcoRI G'AATT_C
Cuts at: 0 56 248 357
Size: 56 192 109
Fragments arranged by size:
192 109 56
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 216 248 357
Size: 216 32 109
Fragments arranged by size:
216 109 32
StyI C'CwwG_G
Cuts at: 0 92 357
Size: 92 265
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 88 120 317 349 357
Size: 88 32 197 32 8
Fragments arranged by size:
197 88 32 32 8
Enzymes that do cut:
ApoI BciVI BmgBI BsaWI BsaXI BsmI BsrDI BstBI
BtgI EcoRI Hin4I StyI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII
BstXI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TatI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI