(Linear) (Six-base) MAPSORT of: KIBB3982.seq Check: 5784 from: 1 to: 357 KIBB3982.orf With 182 enzymes: SgfI * March 3, 2008 10:56 .. ApoI r'AATT_y Cuts at: 0 56 248 357 Size: 56 192 109 Fragments arranged by size: 192 109 56 BciVI GTATCCnnnnn_n' Cuts at: 0 6 357 Size: 6 351 BmgBI CAC'GTC Cuts at: 0 227 357 Size: 227 130 BsaWI w'CCGG_w Cuts at: 0 16 357 Size: 16 341 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 317 347 357 Size: 317 30 10 Fragments arranged by size: 317 30 10 BsmI GAATG_Cn' Cuts at: 0 170 357 Size: 170 187 BsrDI GCAATG_nn' Cuts at: 0 344 357 Size: 344 13 BstBI TT'CG_AA Cuts at: 0 60 162 357 Size: 60 102 195 Fragments arranged by size: 195 102 60 BtgI C'CryG_G Cuts at: 0 29 357 Size: 29 328 EcoRI G'AATT_C Cuts at: 0 56 248 357 Size: 56 192 109 Fragments arranged by size: 192 109 56 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 216 248 357 Size: 216 32 109 Fragments arranged by size: 216 109 32 StyI C'CwwG_G Cuts at: 0 92 357 Size: 92 265 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 88 120 317 349 357 Size: 88 32 197 32 8 Fragments arranged by size: 197 88 32 32 8 Enzymes that do cut: ApoI BciVI BmgBI BsaWI BsaXI BsmI BsrDI BstBI BtgI EcoRI Hin4I StyI TstI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI