Restriction map for pF1KB3982
 (Linear) (Six-base) MAPSORT of: KIBB3982.seq  Check: 5784  from: 1  to: 357



KIBB3982.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:56  ..

ApoI r'AATT_y

Cuts at:      0      56     248     357 
   Size:         56     192     109

  Fragments arranged by size: 

                 192     109      56
 
BciVI GTATCCnnnnn_n'

Cuts at:      0       6     357 
   Size:          6     351
 
BmgBI CAC'GTC

Cuts at:      0     227     357 
   Size:        227     130
 
BsaWI w'CCGG_w

Cuts at:      0      16     357 
   Size:         16     341
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     317     347     357 
   Size:        317      30      10

  Fragments arranged by size: 

                 317      30      10
 
BsmI GAATG_Cn'

Cuts at:      0     170     357 
   Size:        170     187
 
BsrDI GCAATG_nn'

Cuts at:      0     344     357 
   Size:        344      13
 
BstBI TT'CG_AA

Cuts at:      0      60     162     357 
   Size:         60     102     195

  Fragments arranged by size: 

                 195     102      60
 
BtgI C'CryG_G

Cuts at:      0      29     357 
   Size:         29     328
 
EcoRI G'AATT_C

Cuts at:      0      56     248     357 
   Size:         56     192     109

  Fragments arranged by size: 

                 192     109      56
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     216     248     357 
   Size:        216      32     109

  Fragments arranged by size: 

                 216     109      32
 
StyI C'CwwG_G

Cuts at:      0      92     357 
   Size:         92     265
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      88     120     317     349     357 
   Size:         88      32     197      32       8

  Fragments arranged by size: 

                 197      88      32      32       8
 

 Enzymes that do cut:

     ApoI    BciVI    BmgBI    BsaWI    BsaXI     BsmI    BsrDI    BstBI
     BtgI    EcoRI    Hin4I     StyI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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