(Linear) (Six-base) MAPSORT of: KIBB3988.seq  Check: 3062  from: 1  to: 519



KIBB3988.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:37  ..

AflII C'TTAA_G

Cuts at:      0     109     519 
   Size:        109     410
 
AlwNI CAG_nnn'CTG

Cuts at:      0      25     519 
   Size:         25     494
 
ApaLI G'TGCA_C

Cuts at:      0     218     519 
   Size:        218     301
 
ApoI r'AATT_y

Cuts at:      0      94     519 
   Size:         94     425
 
AseI AT'TA_AT

Cuts at:      0     355     519 
   Size:        355     164
 
BanII G_rGCy'C

Cuts at:      0     333     519 
   Size:        333     186
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     196     519 
   Size:        196     323
 
Bme1580I G_kGCm'C

Cuts at:      0     222     519 
   Size:        222     297
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     317     519 
   Size:        317     202
 
BseYI C'CCAG_C

Cuts at:      0      18     519 
   Size:         18     501
 
BsiHKAI G_wGCw'C

Cuts at:      0     222     333     519 
   Size:        222     111     186

  Fragments arranged by size: 

                 222     186     111
 
BsmI GAATG_Cn'

Cuts at:      0     512     519 
   Size:        512       7
 
Bsp1286I G_dGCh'C

Cuts at:      0     222     333     519 
   Size:        222     111     186

  Fragments arranged by size: 

                 222     186     111
 
BsrDI GCAATG_nn'

Cuts at:      0     306     519 
   Size:        306     213
 
EcoICRI GAG'CTC

Cuts at:      0     331     519 
   Size:        331     188
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     317     519 
   Size:        317     202
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      97     254     512     519 
   Size:         97     157     258       7

  Fragments arranged by size: 

                 258     157      97       7
 
SacI G_AGCT'C

Cuts at:      0     333     519 
   Size:        333     186
 
ScaI AGT'ACT

Cuts at:      0     338     519 
   Size:        338     181
 
SexAI A'CCwGG_T

Cuts at:      0     285     519 
   Size:        285     234
 
SmlI C'TyrA_G

Cuts at:      0     109     519 
   Size:        109     410
 
StuI AGG'CCT

Cuts at:      0     320     519 
   Size:        320     199
 
StyI C'CwwG_G

Cuts at:      0     321     519 
   Size:        321     198
 
TatI w'GTAC_w

Cuts at:      0     336     387     519 
   Size:        336      51     132

  Fragments arranged by size: 

                 336     132      51
 

 Enzymes that do cut:

    AflII    AlwNI    ApaLI     ApoI     AseI    BanII    BciVI Bme1580I
     BpmI    BseYI  BsiHKAI     BsmI Bsp1286I    BsrDI  EcoICRI  Eco57MI
     MmeI     SacI     ScaI    SexAI     SmlI     StuI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI     AscI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI
     BcgI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI     BsgI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI