Restriction map for
pF1KB3990
(Linear) (Six-base) MAPSORT of: KIBB3990.seq Check: 3808 from: 1 to: 3174
KIBB3990.orf
With 182 enzymes: SgfI *
October 29, 2009 17:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1644 2054 2191 3174
Size: 1644 410 137 983
Fragments arranged by size:
1644 983 410 137
Acc65I G'GTAC_C
Cuts at: 0 1785 3174
Size: 1785 1389
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1585 1798 2809 2935 3174
Size: 1585 213 1011 126 239
Fragments arranged by size:
1585 1011 239 213 126
AflII C'TTAA_G
Cuts at: 0 258 3174
Size: 258 2916
AflIII A'CryG_T
Cuts at: 0 704 1057 3174
Size: 704 353 2117
Fragments arranged by size:
2117 704 353
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1924 1956 1967 1999 3174
Size: 1924 32 11 32 1175
Fragments arranged by size:
1924 1175 32 32 11
AlwNI CAG_nnn'CTG
Cuts at: 0 627 1436 1581 2286 3174
Size: 627 809 145 705 888
Fragments arranged by size:
888 809 705 627 145
ApaI G_GGCC'C
Cuts at: 0 798 1470 3174
Size: 798 672 1704
Fragments arranged by size:
1704 798 672
ApaLI G'TGCA_C
Cuts at: 0 721 3174
Size: 721 2453
ApoI r'AATT_y
Cuts at: 0 3072 3174
Size: 3072 102
AvaI C'yCGr_G
Cuts at: 0 754 2034 2595 3174
Size: 754 1280 561 579
Fragments arranged by size:
1280 754 579 561
BamHI G'GATC_C
Cuts at: 0 890 3174
Size: 890 2284
BanI G'GyrC_C
Cuts at: 0 741 835 1006 1120 1785 2067 3174
Size: 741 94 171 114 665 282 1107
Fragments arranged by size:
1107 741 665 282 171 114 94
BanII G_rGCy'C
Cuts at: 0 359 798 932 1097 1246 1319 1428 1470
Size: 359 439 134 165 149 73 109 42
Cuts at: 1470 2130 3174
Size: 660 1044
Fragments arranged by size:
1044 660 439 359 165 149 134 109
73 42
BbsI GAAGACnn'nnnn_
Cuts at: 0 2099 3174
Size: 2099 1075
BclI T'GATC_A
Cuts at: 0 2837 3174
Size: 2837 337
BglI GCCn_nnn'nGGC
Cuts at: 0 1069 2055 2295 2710 3174
Size: 1069 986 240 415 464
Fragments arranged by size:
1069 986 464 415 240
BlpI GC'TnA_GC
Cuts at: 0 1313 2263 3174
Size: 1313 950 911
Fragments arranged by size:
1313 950 911
Bme1580I G_kGCm'C
Cuts at: 0 348 725 746 798 823 885 1065 1470
Size: 348 377 21 52 25 62 180 405
Cuts at: 1470 3174
Size: 1704
Fragments arranged by size:
1704 405 377 348 180 62 52 25
21
BmgBI CAC'GTC
Cuts at: 0 1058 2097 2910 3174
Size: 1058 1039 813 264
Fragments arranged by size:
1058 1039 813 264
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2694 3174
Size: 2694 480
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 517 941 1154 1615 2161 2556 2677
Size: 190 327 424 213 461 546 395 121
Cuts at: 2677 2800 3174
Size: 123 374
Fragments arranged by size:
546 461 424 395 374 327 213 190
123 121
Bpu10I CC'TnA_GC
Cuts at: 0 2051 3127 3174
Size: 2051 1076 47
Fragments arranged by size:
2051 1076 47
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 86 185 1429 1627 1838 3174
Size: 86 99 1244 198 211 1336
Fragments arranged by size:
1336 1244 211 198 99 86
BsaI GGTCTCn'nnnn_
Cuts at: 0 2470 3174
Size: 2470 704
BsaAI yAC'GTr
Cuts at: 0 873 3174
Size: 873 2301
BsaBI GATnn'nnATC
Cuts at: 0 1892 3174
Size: 1892 1282
BsaHI Gr'CG_yC
Cuts at: 0 641 1226 2882 3174
Size: 641 585 1656 292
Fragments arranged by size:
1656 641 585 292
BsaWI w'CCGG_w
Cuts at: 0 3083 3174
Size: 3083 91
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1967 1997 3174
Size: 1967 30 1177
Fragments arranged by size:
1967 1177 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 18 493 1552 1897 2158 2449 2505 2559
Size: 18 475 1059 345 261 291 56 54
Cuts at: 2559 3174
Size: 615
Fragments arranged by size:
1059 615 475 345 291 261 56 54
18
BseYI C'CCAG_C
Cuts at: 0 313 863 2334 3174
Size: 313 550 1471 840
Fragments arranged by size:
1471 840 550 313
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1161 2829 3174
Size: 1161 1668 345
Fragments arranged by size:
1668 1161 345
BsiEI CG_ry'CG
Cuts at: 0 440 861 1337 3174
Size: 440 421 476 1837
Fragments arranged by size:
1837 476 440 421
BsiHKAI G_wGCw'C
Cuts at: 0 725 1246 2410 2930 3174
Size: 725 521 1164 520 244
Fragments arranged by size:
1164 725 521 520 244
BsmI GAATG_Cn'
Cuts at: 0 1045 3174
Size: 1045 2129
Bsp1286I G_dGCh'C
Cuts at: 0 348 359 725 746 798 823 885 932
Size: 348 11 366 21 52 25 62 47
Cuts at: 932 1065 1097 1246 1319 1428 1470 2130 2410
Size: 133 32 149 73 109 42 660 280
Cuts at: 2410 2930 3174
Size: 520 244
Fragments arranged by size:
660 520 366 348 280 244 149 133
109 73 62 52 47 42 32 25
21 11
BspEI T'CCGG_A
Cuts at: 0 3083 3174
Size: 3083 91
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1644 1659 2054 2191 2452 3174
Size: 1644 15 395 137 261 722
Fragments arranged by size:
1644 722 395 261 137 15
BsrBI CCG'CTC
Cuts at: 0 243 2055 2586 3174
Size: 243 1812 531 588
Fragments arranged by size:
1812 588 531 243
BsrFI r'CCGG_y
Cuts at: 0 330 2631 3174
Size: 330 2301 543
Fragments arranged by size:
2301 543 330
BsrGI T'GTAC_A
Cuts at: 0 1079 3174
Size: 1079 2095
BssHII G'CGCG_C
Cuts at: 0 1599 3174
Size: 1599 1575
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1019 1581 3174
Size: 1019 562 1593
Fragments arranged by size:
1593 1019 562
BstBI TT'CG_AA
Cuts at: 0 2315 3174
Size: 2315 859
BstXI CCAn_nnnn'nTGG
Cuts at: 0 965 1072 3174
Size: 965 107 2102
Fragments arranged by size:
2102 965 107
BstYI r'GATC_y
Cuts at: 0 890 2750 3174
Size: 890 1860 424
Fragments arranged by size:
1860 890 424
BtgI C'CryG_G
Cuts at: 0 321 1676 1817 1952 2071 2291 3138 3174
Size: 321 1355 141 135 119 220 847 36
Fragments arranged by size:
1355 847 321 220 141 135 119 36
BtsI GCAGTG_nn'
Cuts at: 0 66 194 591 777 2673 3001 3174
Size: 66 128 397 186 1896 328 173
Fragments arranged by size:
1896 397 328 186 173 128 66
ClaI AT'CG_AT
Cuts at: 0 935 3174
Size: 935 2239
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 813 848 3174
Size: 813 35 2326
Fragments arranged by size:
2326 813 35
DraIII CAC_nnn'GTG
Cuts at: 0 876 3174
Size: 876 2298
EaeI y'GGCC_r
Cuts at: 0 338 437 716 1391 2711 2733 2868 3174
Size: 338 99 279 675 1320 22 135 306
Fragments arranged by size:
1320 675 338 306 279 135 99 22
EagI C'GGCC_G
Cuts at: 0 437 3174
Size: 437 2737
EarI CTCTTCn'nnn_
Cuts at: 0 700 2021 2312 2384 2426 3174
Size: 700 1321 291 72 42 748
Fragments arranged by size:
1321 748 700 291 72 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 381 3174
Size: 381 2793
EcoICRI GAG'CTC
Cuts at: 0 1244 3174
Size: 1244 1930
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 517 941 1154 1585 1615 1798 2161
Size: 190 327 424 213 431 30 183 363
Cuts at: 2161 2556 2677 2800 2809 2935 3174
Size: 395 121 123 9 126 239
Fragments arranged by size:
431 424 395 363 327 239 213 190
183 126 123 121 30 9
EcoO109I rG'GnC_Cy
Cuts at: 0 306 1466 1467 1743 3174
Size: 306 1160 1 276 1431
Fragments arranged by size:
1431 1160 306 276 1
EcoRI G'AATT_C
Cuts at: 0 3072 3174
Size: 3072 102
FspI TGC'GCA
Cuts at: 0 367 1627 1763 3174
Size: 367 1260 136 1411
Fragments arranged by size:
1411 1260 367 136
FspAI rTGC'GCAy
Cuts at: 0 367 3174
Size: 367 2807
HaeII r_GCGC'y
Cuts at: 0 141 320 2454 3174
Size: 141 179 2134 720
Fragments arranged by size:
2134 720 179 141
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 84 116 571 603 607 639 1368 1400
Size: 84 32 455 32 4 32 729 32
Cuts at: 1400 3174
Size: 1774
Fragments arranged by size:
1774 729 455 84 32 32 32 32
4
KpnI G_GTAC'C
Cuts at: 0 1789 3174
Size: 1789 1385
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2351 3174
Size: 2351 823
MscI TGG'CCA
Cuts at: 0 1393 2870 3174
Size: 1393 1477 304
Fragments arranged by size:
1477 1393 304
MslI CAynn'nnrTG
Cuts at: 0 658 1087 3174
Size: 658 429 2087
Fragments arranged by size:
2087 658 429
MspA1I CmG'CkG
Cuts at: 0 863 1401 1578 1698 1731 1954 2073 2151
Size: 863 538 177 120 33 223 119 78
Cuts at: 2151 2201 2813 3174
Size: 50 612 361
Fragments arranged by size:
863 612 538 361 223 177 120 119
78 50 33
NaeI GCC'GGC
Cuts at: 0 332 3174
Size: 332 2842
NcoI C'CATG_G
Cuts at: 0 1676 1817 3174
Size: 1676 141 1357
Fragments arranged by size:
1676 1357 141
NgoMIV G'CCGG_C
Cuts at: 0 330 3174
Size: 330 2844
NspI r_CATG'y
Cuts at: 0 555 708 980 2172 2414 2748 2934 3174
Size: 555 153 272 1192 242 334 186 240
Fragments arranged by size:
1192 555 334 272 242 240 186 153
PciI A'CATG_T
Cuts at: 0 704 3174
Size: 704 2470
PfoI T'CCnGG_A
Cuts at: 0 350 1456 2206 3174
Size: 350 1106 750 968
Fragments arranged by size:
1106 968 750 350
PmlI CAC'GTG
Cuts at: 0 873 3174
Size: 873 2301
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1967 1999 2941 2973 3174
Size: 1967 32 942 32 201
Fragments arranged by size:
1967 942 201 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1743 3174
Size: 1743 1431
PshAI GACnn'nnGTC
Cuts at: 0 2462 3174
Size: 2462 712
PspOMI G'GGCC_C
Cuts at: 0 794 1466 3174
Size: 794 672 1708
Fragments arranged by size:
1708 794 672
PspXI vC'TCGA_Gb
Cuts at: 0 2595 3174
Size: 2595 579
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 406 438 3174
Size: 406 32 2736
Fragments arranged by size:
2736 406 32
PstI C_TGCA'G
Cuts at: 0 36 788 1490 1502 2387 3174
Size: 36 752 702 12 885 787
Fragments arranged by size:
885 787 752 702 36 12
PvuII CAG'CTG
Cuts at: 0 1731 2151 3174
Size: 1731 420 1023
Fragments arranged by size:
1731 1023 420
SacI G_AGCT'C
Cuts at: 0 1246 3174
Size: 1246 1928
SacII CC_GC'GG
Cuts at: 0 1955 2074 3174
Size: 1955 119 1100
Fragments arranged by size:
1955 1100 119
ScaI AGT'ACT
Cuts at: 0 1570 3174
Size: 1570 1604
SfcI C'TryA_G
Cuts at: 0 32 784 1486 1498 1944 2383 3041 3174
Size: 32 752 702 12 446 439 658 133
Fragments arranged by size:
752 702 658 446 439 133 32 12
SmlI C'TyrA_G
Cuts at: 0 101 200 258 1444 1642 1853 2595 3174
Size: 101 99 58 1186 198 211 742 579
Fragments arranged by size:
1186 742 579 211 198 101 99 58
SspI AAT'ATT
Cuts at: 0 1197 3174
Size: 1197 1977
StuI AGG'CCT
Cuts at: 0 405 1188 2032 3174
Size: 405 783 844 1142
Fragments arranged by size:
1142 844 783 405
StyI C'CwwG_G
Cuts at: 0 359 1271 1676 1817 2389 2705 3024 3174
Size: 359 912 405 141 572 316 319 150
Fragments arranged by size:
912 572 405 359 319 316 150 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 994 3174
Size: 994 2180
TatI w'GTAC_w
Cuts at: 0 377 413 1079 1568 1919 3174
Size: 377 36 666 489 351 1255
Fragments arranged by size:
1255 666 489 377 351 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 631 655 663 687 3174
Size: 631 24 8 24 2487
Fragments arranged by size:
2487 631 24 24 8
Tth111I GACn'n_nGTC
Cuts at: 0 17 529 2940 3174
Size: 17 512 2411 234
Fragments arranged by size:
2411 512 234 17
XhoI C'TCGA_G
Cuts at: 0 2595 3174
Size: 2595 579
XmnI GAAnn'nnTTC
Cuts at: 0 2360 2956 3174
Size: 2360 596 218
Fragments arranged by size:
2360 596 218
Enzymes that do cut:
AarI Acc65I AcuI AflII AflIII AloI AlwNI ApaI
ApaLI ApoI AvaI BamHI BanI BanII BbsI BclI
BglI BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI
BsrFI BsrGI BssHII BstAPI BstBI BstXI BstYI BtgI
BtsI ClaI CspCI DraIII EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I
KpnI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV
NspI PciI PfoI PmlI PpiI PpuMI PshAI PspOMI
PspXI PsrI PstI PvuII SacI SacII ScaI SfcI
SmlI SspI StuI StyI TaqII TatI TstI Tth111I
XhoI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AgeI AhdI AleI AlfI
AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI
BciVI BfrBI BglII BmrI BmtI BsiWI BsmBI BspHI
BsrDI BssSI BstEII BstZ17I Bsu36I BtgZI DraI DrdI
EcoNI EcoRV FalI FseI HincII HindIII HpaI KasI
MfeI MluI NarI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmeI PsiI PvuI RsrII SalI
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SwaI XbaI XcmI
XmaI ZraI