(Linear) (Six-base) MAPSORT of: KIBB4000.seq  Check: 9286  from: 1  to: 642



KIBB4000.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:57  ..

AleI CACnn'nnGTG

Cuts at:      0     280     642 
   Size:        280     362
 
AlwNI CAG_nnn'CTG

Cuts at:      0      29     181     629     642 
   Size:         29     152     448      13

  Fragments arranged by size: 

                 448     152      29      13
 
AvaI C'yCGr_G

Cuts at:      0     349     642 
   Size:        349     293
 
BanI G'GyrC_C

Cuts at:      0      88     642 
   Size:         88     554
 
BanII G_rGCy'C

Cuts at:      0     356     642 
   Size:        356     286
 
BbvCI CC'TCA_GC

Cuts at:      0     253     642 
   Size:        253     389
 
Bme1580I G_kGCm'C

Cuts at:      0     155     642 
   Size:        155     487
 
Bpu10I CC'TnA_GC

Cuts at:      0     253     642 
   Size:        253     389
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     340     642 
   Size:        340     302
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     550     642 
   Size:        550      92
 
BsaAI yAC'GTr

Cuts at:      0     150     375     642 
   Size:        150     225     267

  Fragments arranged by size: 

                 267     225     150
 
BsaHI Gr'CG_yC

Cuts at:      0     131     642 
   Size:        131     511
 
BsiEI CG_ry'CG

Cuts at:      0     500     642 
   Size:        500     142
 
BsiHKAI G_wGCw'C

Cuts at:      0     356     507     642 
   Size:        356     151     135

  Fragments arranged by size: 

                 356     151     135
 
Bsp1286I G_dGCh'C

Cuts at:      0     155     356     507     642 
   Size:        155     201     151     135

  Fragments arranged by size: 

                 201     155     151     135
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     112     642 
   Size:        112     530
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     207     642 
   Size:        207     435
 
BtgI C'CryG_G

Cuts at:      0      92     227     642 
   Size:         92     135     415

  Fragments arranged by size: 

                 415     135      92
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     144     179     642 
   Size:        144      35     463

  Fragments arranged by size: 

                 463     144      35
 
EcoICRI GAG'CTC

Cuts at:      0     354     642 
   Size:        354     288
 
EcoO109I rG'GnC_Cy

Cuts at:      0     345     395     642 
   Size:        345      50     247

  Fragments arranged by size: 

                 345     247      50
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     222     642 
   Size:        222     420
 
MslI CAynn'nnrTG

Cuts at:      0     280     642 
   Size:        280     362
 
MspA1I CmG'CkG

Cuts at:      0      29     229     252     626     642 
   Size:         29     200      23     374      16

  Fragments arranged by size: 

                 374     200      29      23      16
 
NspI r_CATG'y

Cuts at:      0     369     642 
   Size:        369     273
 
PasI CC'CwG_GG

Cuts at:      0     139     642 
   Size:        139     503
 
PfoI T'CCnGG_A

Cuts at:      0     332     642 
   Size:        332     310
 
PmlI CAC'GTG

Cuts at:      0     375     642 
   Size:        375     267
 
PpuMI rG'GwC_Cy

Cuts at:      0     345     395     642 
   Size:        345      50     247

  Fragments arranged by size: 

                 345     247      50
 
PstI C_TGCA'G

Cuts at:      0      40     642 
   Size:         40     602
 
PvuI CG_AT'CG

Cuts at:      0     500     642 
   Size:        500     142
 
PvuII CAG'CTG

Cuts at:      0      29     252     626     642 
   Size:         29     223     374      16

  Fragments arranged by size: 

                 374     223      29      16
 
SacI G_AGCT'C

Cuts at:      0     356     642 
   Size:        356     286
 
SacII CC_GC'GG

Cuts at:      0     230     642 
   Size:        230     412
 
SanDI GG'GwC_CC

Cuts at:      0     345     395     642 
   Size:        345      50     247

  Fragments arranged by size: 

                 345     247      50
 
SfcI C'TryA_G

Cuts at:      0      36     642 
   Size:         36     606
 
SmlI C'TyrA_G

Cuts at:      0     355     642 
   Size:        355     287
 
SphI G_CATG'C

Cuts at:      0     369     642 
   Size:        369     273
 
StyI C'CwwG_G

Cuts at:      0     428     642 
   Size:        428     214
 

 Enzymes that do cut:

     AleI    AlwNI     AvaI     BanI    BanII    BbvCI Bme1580I   Bpu10I
    BpuEI     BsaI    BsaAI    BsaHI    BsiEI  BsiHKAI Bsp1286I    BspMI
   BstAPI     BtgI    CspCI  EcoICRI EcoO109I     FalI     MslI   MspA1I
     NspI     PasI     PfoI     PmlI    PpuMI     PstI     PvuI    PvuII
     SacI    SacII    SanDI     SfcI     SmlI     SphI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI     BpmI    BsaBI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  Eco57MI    EcoNI    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PciI    PflMI     PmeI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI    RsrII     SalI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI
     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI