Restriction map for
pF1KB4018
(Linear) (Six-base) MAPSORT of: KIBB4018.seq Check: 5410 from: 1 to: 2682
KIBB4018.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AatII G_ACGT'C
Cuts at: 0 89 2682
Size: 89 2593
AccI GT'mk_AC
Cuts at: 0 89 2682
Size: 89 2593
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 613 2059 2682
Size: 613 1446 623
Fragments arranged by size:
1446 623 613
AflII C'TTAA_G
Cuts at: 0 1023 2682
Size: 1023 1659
AflIII A'CryG_T
Cuts at: 0 1221 2682
Size: 1221 1461
AleI CACnn'nnGTG
Cuts at: 0 1151 2682
Size: 1151 1531
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1649 2682
Size: 1649 1033
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1781 1813 2682
Size: 1781 32 869
Fragments arranged by size:
1781 869 32
ApoI r'AATT_y
Cuts at: 0 941 1009 1289 1673 1975 2119 2277 2552
Size: 941 68 280 384 302 144 158 275
Cuts at: 2552 2570 2682
Size: 18 112
Fragments arranged by size:
941 384 302 280 275 158 144 112
68 18
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2185 2218 2682
Size: 2185 33 464
Fragments arranged by size:
2185 464 33
BanI G'GyrC_C
Cuts at: 0 73 1732 2012 2682
Size: 73 1659 280 670
Fragments arranged by size:
1659 670 280 73
BanII G_rGCy'C
Cuts at: 0 1402 1489 2682
Size: 1402 87 1193
Fragments arranged by size:
1402 1193 87
BbsI GAAGACnn'nnnn_
Cuts at: 0 164 1174 1597 2682
Size: 164 1010 423 1085
Fragments arranged by size:
1085 1010 423 164
BfrBI ATG'CAT
Cuts at: 0 1344 2682
Size: 1344 1338
BglII A'GATC_T
Cuts at: 0 1108 2530 2682
Size: 1108 1422 152
Fragments arranged by size:
1422 1108 152
BmgBI CAC'GTC
Cuts at: 0 1224 2682
Size: 1224 1458
BmtI G_CTAG'C
Cuts at: 0 2494 2682
Size: 2494 188
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 521 2365 2682
Size: 521 1844 317
Fragments arranged by size:
1844 521 317
Bpu10I CC'TnA_GC
Cuts at: 0 914 2682
Size: 914 1768
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1171 2682
Size: 1171 1511
BsaAI yAC'GTr
Cuts at: 0 309 2682
Size: 309 2373
BsaBI GATnn'nnATC
Cuts at: 0 302 908 1361 2412 2682
Size: 302 606 453 1051 270
Fragments arranged by size:
1051 606 453 302 270
BsaHI Gr'CG_yC
Cuts at: 0 86 2682
Size: 86 2596
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1934 1964 2682
Size: 1934 30 718
Fragments arranged by size:
1934 718 30
BseYI C'CCAG_C
Cuts at: 0 1401 2682
Size: 1401 1281
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 458 2682
Size: 193 265 2224
Fragments arranged by size:
2224 265 193
BsiHKAI G_wGCw'C
Cuts at: 0 1489 2073 2682
Size: 1489 584 609
Fragments arranged by size:
1489 609 584
BsmI GAATG_Cn'
Cuts at: 0 1344 2516 2682
Size: 1344 1172 166
Fragments arranged by size:
1344 1172 166
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2257 2682
Size: 2257 425
Bsp1286I G_dGCh'C
Cuts at: 0 1402 1489 2073 2682
Size: 1402 87 584 609
Fragments arranged by size:
1402 609 584 87
BspHI T'CATG_A
Cuts at: 0 188 1047 1773 2667 2682
Size: 188 859 726 894 15
Fragments arranged by size:
894 859 726 188 15
BsrGI T'GTAC_A
Cuts at: 0 1810 2651 2682
Size: 1810 841 31
Fragments arranged by size:
1810 841 31
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1501 2682
Size: 1501 1181
BstYI r'GATC_y
Cuts at: 0 1108 1119 2530 2587 2682
Size: 1108 11 1411 57 95
Fragments arranged by size:
1411 1108 95 57 11
Bsu36I CC'TnA_GG
Cuts at: 0 1470 2682
Size: 1470 1212
BtsI GCAGTG_nn'
Cuts at: 0 358 602 605 1100 2682
Size: 358 244 3 495 1582
Fragments arranged by size:
1582 495 358 244 3
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1283 1318 2682
Size: 1283 35 1364
Fragments arranged by size:
1364 1283 35
DraIII CAC_nnn'GTG
Cuts at: 0 747 2682
Size: 747 1935
EaeI y'GGCC_r
Cuts at: 0 999 2682
Size: 999 1683
EarI CTCTTCn'nnn_
Cuts at: 0 2292 2682
Size: 2292 390
EcoICRI GAG'CTC
Cuts at: 0 1487 2682
Size: 1487 1195
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 521 613 2059 2365 2682
Size: 521 92 1446 306 317
Fragments arranged by size:
1446 521 317 306 92
EcoRI G'AATT_C
Cuts at: 0 941 2682
Size: 941 1741
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1341 2057 2682
Size: 1341 716 625
Fragments arranged by size:
1341 716 625
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1208 1240 1246 1278 2256 2288 2342 2374
Size: 1208 32 6 32 978 32 54 32
Cuts at: 2374 2682
Size: 308
Fragments arranged by size:
1208 978 308 54 32 32 32 32
6
HincII GTy'rAC
Cuts at: 0 108 1528 2682
Size: 108 1420 1154
Fragments arranged by size:
1420 1154 108
HindIII A'AGCT_T
Cuts at: 0 253 1859 2682
Size: 253 1606 823
Fragments arranged by size:
1606 823 253
HpaI GTT'AAC
Cuts at: 0 108 2682
Size: 108 2574
MfeI C'AATT_G
Cuts at: 0 491 1833 2682
Size: 491 1342 849
Fragments arranged by size:
1342 849 491
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 836 1636 2682
Size: 836 800 1046
Fragments arranged by size:
1046 836 800
MscI TGG'CCA
Cuts at: 0 1001 2682
Size: 1001 1681
MslI CAynn'nnrTG
Cuts at: 0 1151 1631 1689 2682
Size: 1151 480 58 993
Fragments arranged by size:
1151 993 480 58
MspA1I CmG'CkG
Cuts at: 0 71 550 2682
Size: 71 479 2132
Fragments arranged by size:
2132 479 71
NdeI CA'TA_TG
Cuts at: 0 342 1628 2682
Size: 342 1286 1054
Fragments arranged by size:
1286 1054 342
NheI G'CTAG_C
Cuts at: 0 2490 2682
Size: 2490 192
NsiI A_TGCA'T
Cuts at: 0 1346 2682
Size: 1346 1336
NspI r_CATG'y
Cuts at: 0 1640 2682
Size: 1640 1042
PacI TTA_AT'TAA
Cuts at: 0 2132 2682
Size: 2132 550
PshAI GACnn'nnGTC
Cuts at: 0 321 2682
Size: 321 2361
PstI C_TGCA'G
Cuts at: 0 365 2682
Size: 365 2317
PvuII CAG'CTG
Cuts at: 0 550 2682
Size: 550 2132
SacI G_AGCT'C
Cuts at: 0 1489 2682
Size: 1489 1193
ScaI AGT'ACT
Cuts at: 0 1720 2682
Size: 1720 962
SfcI C'TryA_G
Cuts at: 0 361 2682
Size: 361 2321
SmlI C'TyrA_G
Cuts at: 0 1023 1186 2682
Size: 1023 163 1496
Fragments arranged by size:
1496 1023 163
SspI AAT'ATT
Cuts at: 0 216 2682
Size: 216 2466
StyI C'CwwG_G
Cuts at: 0 1149 1969 2400 2682
Size: 1149 820 431 282
Fragments arranged by size:
1149 820 431 282
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 224 668 1621 1649 2682
Size: 224 444 953 28 1033
Fragments arranged by size:
1033 953 444 224 28
TatI w'GTAC_w
Cuts at: 0 1718 1810 2651 2682
Size: 1718 92 841 31
Fragments arranged by size:
1718 841 92 31
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1934 1966 2682
Size: 1934 32 716
Fragments arranged by size:
1934 716 32
Tth111I GACn'n_nGTC
Cuts at: 0 834 2596 2682
Size: 834 1762 86
Fragments arranged by size:
1762 834 86
XmnI GAAnn'nnTTC
Cuts at: 0 508 2682
Size: 508 2174
ZraI GAC'GTC
Cuts at: 0 87 2682
Size: 87 2595
Enzymes that do cut:
AatII AccI AcuI AflII AflIII AleI AlfI AloI
ApoI BaeI BanI BanII BbsI BfrBI BglII BmgBI
BmtI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrGI
BstXI BstYI Bsu36I BtsI CspCI DraIII EaeI EarI
EcoICRI Eco57MI EcoRI FalI Hin4I HincII HindIII HpaI
MfeI MmeI MscI MslI MspA1I NdeI NheI NsiI
NspI PacI PshAI PstI PvuII SacI ScaI SfcI
SmlI SspI StyI TaqII TatI TstI Tth111I XmnI
ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AgeI AhdI AlwNI ApaI
ApaLI AscI AseI AsiSI AvaI AvrII BamHI BbeI
BbvCI BcgI BciVI BclI BglI BlpI Bme1580I BmrI
BplI BsaI BsaWI BseRI BsiEI BsiWI BspEI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstZ17I BtgI BtgZI ClaI DraI DrdI EagI EciI
EcoNI EcoO109I EcoRV FseI FspI FspAI HaeII KasI
KpnI MluI NaeI NarI NcoI NgoMIV NotI NruI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI SwaI XbaI
XcmI XhoI XmaI