Restriction map for
pF1KB4019
(Linear) (Six-base) MAPSORT of: KIBB4019.seq Check: 6916 from: 1 to: 1290
KIBB4019.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AflIII A'CryG_T
Cuts at: 0 1016 1290
Size: 1016 274
AleI CACnn'nnGTG
Cuts at: 0 532 1290
Size: 532 758
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 47 1290
Size: 47 1243
ApaI G_GGCC'C
Cuts at: 0 1083 1290
Size: 1083 207
ApoI r'AATT_y
Cuts at: 0 714 889 1290
Size: 714 175 401
Fragments arranged by size:
714 401 175
BanII G_rGCy'C
Cuts at: 0 285 1083 1290
Size: 285 798 207
Fragments arranged by size:
798 285 207
BciVI GTATCCnnnnn_n'
Cuts at: 0 283 1107 1141 1290
Size: 283 824 34 149
Fragments arranged by size:
824 283 149 34
BfrBI ATG'CAT
Cuts at: 0 24 1290
Size: 24 1266
Bme1580I G_kGCm'C
Cuts at: 0 1083 1290
Size: 1083 207
BmrI ACTGGGnnnn_n'
Cuts at: 0 776 1290
Size: 776 514
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 475 1290
Size: 475 815
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 428 514 1049 1290
Size: 428 86 535 241
Fragments arranged by size:
535 428 241 86
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 628 1290
Size: 628 662
BsaAI yAC'GTr
Cuts at: 0 534 656 1290
Size: 534 122 634
Fragments arranged by size:
634 534 122
BsaWI w'CCGG_w
Cuts at: 0 866 1290
Size: 866 424
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1082 1112 1290
Size: 1082 30 178
Fragments arranged by size:
1082 178 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 293 304 814 1290
Size: 293 11 510 476
Fragments arranged by size:
510 476 293 11
BseYI C'CCAG_C
Cuts at: 0 34 68 308 1290
Size: 34 34 240 982
Fragments arranged by size:
982 240 34 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1069 1290
Size: 1069 221
BsiHKAI G_wGCw'C
Cuts at: 0 655 1290
Size: 655 635
BsmI GAATG_Cn'
Cuts at: 0 24 438 1290
Size: 24 414 852
Fragments arranged by size:
852 414 24
BsmBI CGTCTCn'nnnn_
Cuts at: 0 702 1290
Size: 702 588
Bsp1286I G_dGCh'C
Cuts at: 0 285 655 1083 1290
Size: 285 370 428 207
Fragments arranged by size:
428 370 285 207
BspHI T'CATG_A
Cuts at: 0 1040 1290
Size: 1040 250
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 406 1290
Size: 406 884
BsrBI CCG'CTC
Cuts at: 0 594 1290
Size: 594 696
BsrGI T'GTAC_A
Cuts at: 0 1178 1290
Size: 1178 112
BtgI C'CryG_G
Cuts at: 0 954 1290
Size: 954 336
BtsI GCAGTG_nn'
Cuts at: 0 932 1272 1290
Size: 932 340 18
Fragments arranged by size:
932 340 18
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 798 833 1290
Size: 798 35 457
Fragments arranged by size:
798 457 35
DraI TTT'AAA
Cuts at: 0 203 1290
Size: 203 1087
EaeI y'GGCC_r
Cuts at: 0 47 1290
Size: 47 1243
EarI CTCTTCn'nnn_
Cuts at: 0 700 1290
Size: 700 590
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 383 717 1290
Size: 383 334 573
Fragments arranged by size:
573 383 334
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 428 514 1049 1290
Size: 428 86 535 241
Fragments arranged by size:
535 428 241 86
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1253 1290
Size: 1253 37
EcoO109I rG'GnC_Cy
Cuts at: 0 355 1079 1258 1290
Size: 355 724 179 32
Fragments arranged by size:
724 355 179 32
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 243 1290
Size: 243 1047
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1167 1199 1290
Size: 1167 32 91
Fragments arranged by size:
1167 91 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1068 1290
Size: 1068 222
MslI CAynn'nnrTG
Cuts at: 0 29 532 634 1045 1290
Size: 29 503 102 411 245
Fragments arranged by size:
503 411 245 102 29
MspA1I CmG'CkG
Cuts at: 0 312 385 394 928 1290
Size: 312 73 9 534 362
Fragments arranged by size:
534 362 312 73 9
NcoI C'CATG_G
Cuts at: 0 954 1290
Size: 954 336
NsiI A_TGCA'T
Cuts at: 0 26 1290
Size: 26 1264
NspI r_CATG'y
Cuts at: 0 199 333 1020 1290
Size: 199 134 687 270
Fragments arranged by size:
687 270 199 134
PciI A'CATG_T
Cuts at: 0 1016 1290
Size: 1016 274
PflMI CCAn_nnn'nTGG
Cuts at: 0 171 1290
Size: 171 1119
PmlI CAC'GTG
Cuts at: 0 534 656 1290
Size: 534 122 634
Fragments arranged by size:
634 534 122
PspOMI G'GGCC_C
Cuts at: 0 1079 1290
Size: 1079 211
PvuII CAG'CTG
Cuts at: 0 312 928 1290
Size: 312 616 362
Fragments arranged by size:
616 362 312
SfcI C'TryA_G
Cuts at: 0 1250 1290
Size: 1250 40
SmlI C'TyrA_G
Cuts at: 0 643 1290
Size: 643 647
SspI AAT'ATT
Cuts at: 0 751 909 1290
Size: 751 158 381
Fragments arranged by size:
751 381 158
StuI AGG'CCT
Cuts at: 0 511 1290
Size: 511 779
StyI C'CwwG_G
Cuts at: 0 954 1074 1290
Size: 954 120 216
Fragments arranged by size:
954 216 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 112 544 1290
Size: 112 432 746
Fragments arranged by size:
746 432 112
TatI w'GTAC_w
Cuts at: 0 1178 1290
Size: 1178 112
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1082 1114 1290
Size: 1082 32 176
Fragments arranged by size:
1082 176 32
Enzymes that do cut:
AflIII AleI AlfI ApaI ApoI BanII BciVI BfrBI
Bme1580I BmrI BplI BpmI BpuEI BsaAI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrGI BtgI BtsI CspCI DraI EaeI
EarI EciI Eco57MI EcoNI EcoO109I FalI Hin4I MmeI
MslI MspA1I NcoI NsiI NspI PciI PflMI PmlI
PspOMI PvuII SfcI SmlI SspI StuI StyI TaqII
TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AloI AlwNI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbsI BbvCI
BcgI BclI BglI BglII BlpI BmgBI BmtI Bpu10I
BsaI BsaBI BsaHI BsiEI BsiWI BspEI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgZI ClaI DraIII DrdI EagI EcoICRI EcoRI
EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MscI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI PasI PfoI PmeI
PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SwaI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI