Restriction map for
pF1KB4020
(Linear) (Six-base) MAPSORT of: KIBB4020.seq Check: 1982 from: 1 to: 1992
KIBB4020.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1467 1992
Size: 1467 525
AccI GT'mk_AC
Cuts at: 0 131 1992
Size: 131 1861
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 421 532 1486 1992
Size: 218 203 111 954 506
Fragments arranged by size:
954 506 218 203 111
AgeI A'CCGG_T
Cuts at: 0 1442 1992
Size: 1442 550
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1645 1992
Size: 1645 347
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 599 631 1992
Size: 599 32 1361
Fragments arranged by size:
1361 599 32
AlwNI CAG_nnn'CTG
Cuts at: 0 741 1065 1504 1642 1992
Size: 741 324 439 138 350
Fragments arranged by size:
741 439 350 324 138
ApaI G_GGCC'C
Cuts at: 0 750 1992
Size: 750 1242
AscI GG'CGCG_CC
Cuts at: 0 21 1992
Size: 21 1971
AvaI C'yCGr_G
Cuts at: 0 253 1716 1992
Size: 253 1463 276
Fragments arranged by size:
1463 276 253
AvrII C'CTAG_G
Cuts at: 0 525 1992
Size: 525 1467
BanII G_rGCy'C
Cuts at: 0 113 254 750 1815 1972 1992
Size: 113 141 496 1065 157 20
Fragments arranged by size:
1065 496 157 141 113 20
BbsI GAAGACnn'nnnn_
Cuts at: 0 799 1992
Size: 799 1193
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 876 910 1992
Size: 876 34 1082
Fragments arranged by size:
1082 876 34
BglI GCCn_nnn'nGGC
Cuts at: 0 244 949 1789 1992
Size: 244 705 840 203
Fragments arranged by size:
840 705 244 203
BlpI GC'TnA_GC
Cuts at: 0 316 916 1992
Size: 316 600 1076
Fragments arranged by size:
1076 600 316
Bme1580I G_kGCm'C
Cuts at: 0 750 1992
Size: 750 1242
BmrI ACTGGGnnnn_n'
Cuts at: 0 1610 1992
Size: 1610 382
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 467 1992
Size: 467 1525
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 430 493 808 910 988 1282 1836
Size: 331 99 63 315 102 78 294 554
Cuts at: 1836 1935 1992
Size: 99 57
Fragments arranged by size:
554 331 315 294 102 99 99 78
63 57
Bpu10I CC'TnA_GC
Cuts at: 0 389 1806 1992
Size: 389 1417 186
Fragments arranged by size:
1417 389 186
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 535 1992
Size: 535 1457
BsaI GGTCTCn'nnnn_
Cuts at: 0 643 661 956 1992
Size: 643 18 295 1036
Fragments arranged by size:
1036 643 295 18
BsaWI w'CCGG_w
Cuts at: 0 1442 1992
Size: 1442 550
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 25 1333 1363 1684 1714 1802 1832 1992
Size: 25 1308 30 321 30 88 30 160
Fragments arranged by size:
1308 321 160 88 30 30 30 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 346 652 883 1162 1165 1342 1354 1824
Size: 346 306 231 279 3 177 12 470
Cuts at: 1824 1992
Size: 168
Fragments arranged by size:
470 346 306 279 231 177 168 12
3
BseYI C'CCAG_C
Cuts at: 0 14 749 911 1171 1471 1504 1992
Size: 14 735 162 260 300 33 488
Fragments arranged by size:
735 488 300 260 162 33 14
BsiEI CG_ry'CG
Cuts at: 0 141 1992
Size: 141 1851
BsiHKAI G_wGCw'C
Cuts at: 0 113 254 448 1992
Size: 113 141 194 1544
Fragments arranged by size:
1544 194 141 113
BsiWI C'GTAC_G
Cuts at: 0 1579 1992
Size: 1579 413
Bsp1286I G_dGCh'C
Cuts at: 0 113 254 448 750 1815 1972 1992
Size: 113 141 194 302 1065 157 20
Fragments arranged by size:
1065 302 194 157 141 113 20
BspHI T'CATG_A
Cuts at: 0 663 1992
Size: 663 1329
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 110 1467 1688 1748 1777 1992
Size: 110 1357 221 60 29 215
Fragments arranged by size:
1357 221 215 110 60 29
BsrBI CCG'CTC
Cuts at: 0 990 1026 1931 1992
Size: 990 36 905 61
Fragments arranged by size:
990 905 61 36
BsrFI r'CCGG_y
Cuts at: 0 716 1001 1442 1992
Size: 716 285 441 550
Fragments arranged by size:
716 550 441 285
BssHII G'CGCG_C
Cuts at: 0 21 294 352 1992
Size: 21 273 58 1640
Fragments arranged by size:
1640 273 58 21
BssSI C'ACGA_G
Cuts at: 0 865 1992
Size: 865 1127
BstAPI GCAn_nnn'nTGC
Cuts at: 0 492 741 1992
Size: 492 249 1251
Fragments arranged by size:
1251 492 249
BstEII G'GTnAC_C
Cuts at: 0 373 1923 1992
Size: 373 1550 69
Fragments arranged by size:
1550 373 69
BstYI r'GATC_y
Cuts at: 0 1115 1992
Size: 1115 877
BtgI C'CryG_G
Cuts at: 0 1194 1322 1388 1695 1992
Size: 1194 128 66 307 297
Fragments arranged by size:
1194 307 297 128 66
BtsI GCAGTG_nn'
Cuts at: 0 446 1704 1887 1899 1992
Size: 446 1258 183 12 93
Fragments arranged by size:
1258 446 183 93 12
DraIII CAC_nnn'GTG
Cuts at: 0 1231 1992
Size: 1231 761
DrdI GACnn_nn'nnGTC
Cuts at: 0 1921 1992
Size: 1921 71
EaeI y'GGCC_r
Cuts at: 0 506 1034 1197 1319 1613 1992
Size: 506 528 163 122 294 379
Fragments arranged by size:
528 506 379 294 163 122
EarI CTCTTCn'nnn_
Cuts at: 0 194 1848 1992
Size: 194 1654 144
Fragments arranged by size:
1654 194 144
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 739 1030 1838 1992
Size: 739 291 808 154
Fragments arranged by size:
808 739 291 154
EcoICRI GAG'CTC
Cuts at: 0 111 252 1992
Size: 111 141 1740
Fragments arranged by size:
1740 141 111
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 331 421 430 493 532 808 910
Size: 218 113 90 9 63 39 276 102
Cuts at: 910 988 1282 1486 1836 1935 1992
Size: 78 294 204 350 99 57
Fragments arranged by size:
350 294 276 218 204 113 102 99
90 78 63 57 39 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 529 1992
Size: 529 1463
EcoO109I rG'GnC_Cy
Cuts at: 0 99 405 1722 1766 1992
Size: 99 306 1317 44 226
Fragments arranged by size:
1317 306 226 99 44
FspI TGC'GCA
Cuts at: 0 1363 1621 1992
Size: 1363 258 371
Fragments arranged by size:
1363 371 258
HaeII r_GCGC'y
Cuts at: 0 660 1413 1992
Size: 660 753 579
Fragments arranged by size:
753 660 579
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 27 372 404 889 921 1369 1401 1777
Size: 27 345 32 485 32 448 32 376
Cuts at: 1777 1809 1992
Size: 32 183
Fragments arranged by size:
485 448 376 345 183 32 32 32
32 27
HindIII A'AGCT_T
Cuts at: 0 511 955 1992
Size: 511 444 1037
Fragments arranged by size:
1037 511 444
MfeI C'AATT_G
Cuts at: 0 1395 1992
Size: 1395 597
MscI TGG'CCA
Cuts at: 0 508 1615 1992
Size: 508 1107 377
Fragments arranged by size:
1107 508 377
MspA1I CmG'CkG
Cuts at: 0 18 35 60 309 936 1065 1729 1802
Size: 18 17 25 249 627 129 664 73
Cuts at: 1802 1992
Size: 190
Fragments arranged by size:
664 627 249 190 129 73 25 18
17
NaeI GCC'GGC
Cuts at: 0 718 1003 1992
Size: 718 285 989
Fragments arranged by size:
989 718 285
NcoI C'CATG_G
Cuts at: 0 1388 1992
Size: 1388 604
NgoMIV G'CCGG_C
Cuts at: 0 716 1001 1992
Size: 716 285 991
Fragments arranged by size:
991 716 285
PflMI CCAn_nnn'nTGG
Cuts at: 0 1389 1992
Size: 1389 603
PpuMI rG'GwC_Cy
Cuts at: 0 99 405 1722 1766 1992
Size: 99 306 1317 44 226
Fragments arranged by size:
1317 306 226 99 44
PspOMI G'GGCC_C
Cuts at: 0 746 1992
Size: 746 1246
PstI C_TGCA'G
Cuts at: 0 89 107 308 593 737 878 1500 1711
Size: 89 18 201 285 144 141 622 211
Cuts at: 1711 1984 1992
Size: 273 8
Fragments arranged by size:
622 285 273 211 201 144 141 89
18 8
PvuII CAG'CTG
Cuts at: 0 309 936 1065 1992
Size: 309 627 129 927
Fragments arranged by size:
927 627 309 129
SacI G_AGCT'C
Cuts at: 0 113 254 1992
Size: 113 141 1738
Fragments arranged by size:
1738 141 113
SanDI GG'GwC_CC
Cuts at: 0 1722 1992
Size: 1722 270
SapI GCTCTTCn'nnn_
Cuts at: 0 1848 1992
Size: 1848 144
SbfI CC_TGCA'GG
Cuts at: 0 107 1992
Size: 107 1885
SexAI A'CCwGG_T
Cuts at: 0 761 848 1532 1992
Size: 761 87 684 460
Fragments arranged by size:
761 684 460 87
SfcI C'TryA_G
Cuts at: 0 85 103 304 589 630 733 874 1496
Size: 85 18 201 285 41 103 141 622
Cuts at: 1496 1707 1980 1992
Size: 211 273 12
Fragments arranged by size:
622 285 273 211 201 141 103 85
41 18 12
SmlI C'TyrA_G
Cuts at: 0 514 1992
Size: 514 1478
StyI C'CwwG_G
Cuts at: 0 287 428 525 944 1388 1992
Size: 287 141 97 419 444 604
Fragments arranged by size:
604 444 419 287 141 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1528 1992
Size: 1528 464
TatI w'GTAC_w
Cuts at: 0 1733 1992
Size: 1733 259
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 687 719 1684 1716 1802 1834 1992
Size: 687 32 965 32 86 32 158
Fragments arranged by size:
965 687 158 86 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 9 1992
Size: 9 1983
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1228 1623 1992
Size: 1228 395 369
Fragments arranged by size:
1228 395 369
Enzymes that do cut:
AarI AccI AcuI AgeI AlfI AloI AlwNI ApaI
AscI AvaI AvrII BanII BbsI BcgI BglI BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsiWI Bsp1286I BspHI
BspMI BsrBI BsrFI BssHII BssSI BstAPI BstEII BstYI
BtgI BtsI DraIII DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HindIII MfeI
MscI MspA1I NaeI NcoI NgoMIV PflMI PpuMI PspOMI
PstI PvuII SacI SanDI SapI SbfI SexAI SfcI
SmlI StyI TaqII TatI TstI Tth111I XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AhdI AleI
ApaLI ApoI AseI AsiSI BaeI BamHI BanI BbeI
BbvCI BciVI BclI BfrBI BglII BmgBI BmtI BsaAI
BsaBI BsaHI BsgI BsmI BsmBI BspEI BsrDI BsrGI
BstBI BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
EagI EcoRI EcoRV FalI FseI FspAI HincII HpaI
KasI KpnI MluI MmeI MslI NarI NdeI NheI
NotI NruI NsiI NspI PacI PasI PciI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
XbaI XhoI XmaI XmnI ZraI