Restriction map for
pF1KB4044
(Linear) (Six-base) MAPSORT of: KIBB4044.seq Check: 9414 from: 1 to: 1548
KIBB4044.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 593 1548
Size: 593 955
AflIII A'CryG_T
Cuts at: 0 282 428 1372 1548
Size: 282 146 944 176
Fragments arranged by size:
944 282 176 146
AlwNI CAG_nnn'CTG
Cuts at: 0 585 1516 1548
Size: 585 931 32
Fragments arranged by size:
931 585 32
ApaLI G'TGCA_C
Cuts at: 0 1440 1548
Size: 1440 108
ApoI r'AATT_y
Cuts at: 0 308 856 991 1548
Size: 308 548 135 557
Fragments arranged by size:
557 548 308 135
AseI AT'TA_AT
Cuts at: 0 92 1548
Size: 92 1456
AvrII C'CTAG_G
Cuts at: 0 1465 1548
Size: 1465 83
BamHI G'GATC_C
Cuts at: 0 540 1548
Size: 540 1008
BanII G_rGCy'C
Cuts at: 0 747 1272 1504 1548
Size: 747 525 232 44
Fragments arranged by size:
747 525 232 44
BglII A'GATC_T
Cuts at: 0 815 1548
Size: 815 733
BlpI GC'TnA_GC
Cuts at: 0 972 1548
Size: 972 576
Bme1580I G_kGCm'C
Cuts at: 0 794 1444 1548
Size: 794 650 104
Fragments arranged by size:
794 650 104
BmgBI CAC'GTC
Cuts at: 0 429 1548
Size: 429 1119
BmrI ACTGGGnnnn_n'
Cuts at: 0 1299 1548
Size: 1299 249
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 556 1548
Size: 58 498 992
Fragments arranged by size:
992 498 58
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1123 1548
Size: 1123 425
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 755 1548
Size: 755 793
BseYI C'CCAG_C
Cuts at: 0 97 1274 1548
Size: 97 1177 274
Fragments arranged by size:
1177 274 97
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 1548
Size: 571 977
BsiHKAI G_wGCw'C
Cuts at: 0 361 512 747 1444 1548
Size: 361 151 235 697 104
Fragments arranged by size:
697 361 235 151 104
BsmI GAATG_Cn'
Cuts at: 0 1438 1548
Size: 1438 110
Bsp1286I G_dGCh'C
Cuts at: 0 361 512 747 794 1272 1444 1504 1548
Size: 361 151 235 47 478 172 60 44
Fragments arranged by size:
478 361 235 172 151 60 47 44
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 593 1336 1548
Size: 593 743 212
Fragments arranged by size:
743 593 212
BsrBI CCG'CTC
Cuts at: 0 1086 1548
Size: 1086 462
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1485 1548
Size: 1485 63
BstBI TT'CG_AA
Cuts at: 0 606 1548
Size: 606 942
BstYI r'GATC_y
Cuts at: 0 540 551 815 1548
Size: 540 11 264 733
Fragments arranged by size:
733 540 264 11
BtgI C'CryG_G
Cuts at: 0 794 1548
Size: 794 754
BtsI GCAGTG_nn'
Cuts at: 0 498 1548
Size: 498 1050
DraI TTT'AAA
Cuts at: 0 74 335 401 1425 1548
Size: 74 261 66 1024 123
Fragments arranged by size:
1024 261 123 74 66
EaeI y'GGCC_r
Cuts at: 0 483 1453 1548
Size: 483 970 95
Fragments arranged by size:
970 483 95
EarI CTCTTCn'nnn_
Cuts at: 0 1039 1160 1548
Size: 1039 121 388
Fragments arranged by size:
1039 388 121
EcoICRI GAG'CTC
Cuts at: 0 745 1548
Size: 745 803
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 556 1548
Size: 58 498 992
Fragments arranged by size:
992 498 58
EcoO109I rG'GnC_Cy
Cuts at: 0 1405 1513 1548
Size: 1405 108 35
Fragments arranged by size:
1405 108 35
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 427 697 1548
Size: 427 270 851
Fragments arranged by size:
851 427 270
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1231 1263 1548
Size: 1231 32 285
Fragments arranged by size:
1231 285 32
HincII GTy'rAC
Cuts at: 0 1017 1548
Size: 1017 531
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 1548
Size: 223 1325
MscI TGG'CCA
Cuts at: 0 485 1548
Size: 485 1063
MslI CAynn'nnrTG
Cuts at: 0 281 302 422 1548
Size: 281 21 120 1126
Fragments arranged by size:
1126 281 120 21
MspA1I CmG'CkG
Cuts at: 0 1079 1274 1548
Size: 1079 195 274
Fragments arranged by size:
1079 274 195
NcoI C'CATG_G
Cuts at: 0 794 1548
Size: 794 754
NspI r_CATG'y
Cuts at: 0 286 301 1376 1486 1548
Size: 286 15 1075 110 62
Fragments arranged by size:
1075 286 110 62 15
PciI A'CATG_T
Cuts at: 0 282 1372 1548
Size: 282 1090 176
Fragments arranged by size:
1090 282 176
PflMI CCAn_nnn'nTGG
Cuts at: 0 534 1548
Size: 534 1014
PstI C_TGCA'G
Cuts at: 0 384 618 1548
Size: 384 234 930
Fragments arranged by size:
930 384 234
SacI G_AGCT'C
Cuts at: 0 747 1548
Size: 747 801
SfcI C'TryA_G
Cuts at: 0 380 614 1548
Size: 380 234 934
Fragments arranged by size:
934 380 234
SmlI C'TyrA_G
Cuts at: 0 1138 1548
Size: 1138 410
StuI AGG'CCT
Cuts at: 0 166 1548
Size: 166 1382
StyI C'CwwG_G
Cuts at: 0 24 794 963 1109 1465 1548
Size: 24 770 169 146 356 83
Fragments arranged by size:
770 356 169 146 83 24
TatI w'GTAC_w
Cuts at: 0 130 1058 1548
Size: 130 928 490
Fragments arranged by size:
928 490 130
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 624 656 694 726 1423 1455 1548
Size: 624 32 38 32 697 32 93
Fragments arranged by size:
697 624 93 38 32 32 32
XbaI T'CTAG_A
Cuts at: 0 1026 1548
Size: 1026 522
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 666 1141 1548
Size: 666 475 407
Fragments arranged by size:
666 475 407
XmnI GAAnn'nnTTC
Cuts at: 0 1169 1548
Size: 1169 379
Enzymes that do cut:
AarI AflIII AlwNI ApaLI ApoI AseI AvrII BamHI
BanII BglII BlpI Bme1580I BmgBI BmrI BpmI BpuEI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI
BstAPI BstBI BstYI BtgI BtsI DraI EaeI EarI
EcoICRI Eco57MI EcoO109I FalI Hin4I HincII MmeI MscI
MslI MspA1I NcoI NspI PciI PflMI PstI SacI
SfcI SmlI StuI StyI TatI TstI XbaI XcmI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI AscI AsiSI AvaI
BaeI BanI BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BmtI BplI Bpu10I BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BspHI
BsrDI BsrFI BsrGI BssHII BssSI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI CspCI DraIII DrdI EagI EciI
EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TaqII Tth111I
XhoI XmaI ZraI