Restriction map for
pF1KB4046
(Linear) (Six-base) MAPSORT of: KIBB4046.seq Check: 9774 from: 1 to: 1335
KIBB4046.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1095 1335
Size: 1095 240
AccI GT'mk_AC
Cuts at: 0 1085 1162 1335
Size: 1085 77 173
Fragments arranged by size:
1085 173 77
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 706 1335
Size: 25 681 629
Fragments arranged by size:
681 629 25
AfeI AGC'GCT
Cuts at: 0 123 1335
Size: 123 1212
AflII C'TTAA_G
Cuts at: 0 1219 1335
Size: 1219 116
AhdI GACnn_n'nnGTC
Cuts at: 0 1221 1335
Size: 1221 114
AleI CACnn'nnGTG
Cuts at: 0 1305 1335
Size: 1305 30
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 529 1335
Size: 529 806
AlwNI CAG_nnn'CTG
Cuts at: 0 100 1335
Size: 100 1235
ApaLI G'TGCA_C
Cuts at: 0 1015 1335
Size: 1015 320
AvaI C'yCGr_G
Cuts at: 0 152 1335
Size: 152 1183
BanI G'GyrC_C
Cuts at: 0 255 513 1293 1335
Size: 255 258 780 42
Fragments arranged by size:
780 258 255 42
BanII G_rGCy'C
Cuts at: 0 401 824 842 1335
Size: 401 423 18 493
Fragments arranged by size:
493 423 401 18
BbeI G_GCGC'C
Cuts at: 0 1297 1335
Size: 1297 38
BbsI GAAGACnn'nnnn_
Cuts at: 0 579 1335
Size: 579 756
BglI GCCn_nnn'nGGC
Cuts at: 0 846 1335
Size: 846 489
BglII A'GATC_T
Cuts at: 0 920 1335
Size: 920 415
BlpI GC'TnA_GC
Cuts at: 0 395 830 1335
Size: 395 435 505
Fragments arranged by size:
505 435 395
Bme1580I G_kGCm'C
Cuts at: 0 260 434 1019 1335
Size: 260 174 585 316
Fragments arranged by size:
585 316 260 174
BmgBI CAC'GTC
Cuts at: 0 1307 1335
Size: 1307 28
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1090 1335
Size: 1090 245
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 655 856 1335
Size: 214 441 201 479
Fragments arranged by size:
479 441 214 201
Bpu10I CC'TnA_GC
Cuts at: 0 499 764 1335
Size: 499 265 571
Fragments arranged by size:
571 499 265
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 909 970 1335
Size: 909 61 365
Fragments arranged by size:
909 365 61
BsaBI GATnn'nnATC
Cuts at: 0 319 1335
Size: 319 1016
BsaHI Gr'CG_yC
Cuts at: 0 179 752 1294 1335
Size: 179 573 542 41
Fragments arranged by size:
573 542 179 41
BsaWI w'CCGG_w
Cuts at: 0 1130 1335
Size: 1130 205
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 829 859 1335
Size: 829 30 476
Fragments arranged by size:
829 476 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 64 451 1335
Size: 64 387 884
Fragments arranged by size:
884 387 64
BseYI C'CCAG_C
Cuts at: 0 93 278 569 576 1025 1097 1140 1335
Size: 93 185 291 7 449 72 43 195
Fragments arranged by size:
449 291 195 185 93 72 43 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1177 1335
Size: 1177 158
BsiEI CG_ry'CG
Cuts at: 0 614 883 1335
Size: 614 269 452
Fragments arranged by size:
614 452 269
BsiHKAI G_wGCw'C
Cuts at: 0 401 824 1019 1095 1335
Size: 401 423 195 76 240
Fragments arranged by size:
423 401 240 195 76
BsmI GAATG_Cn'
Cuts at: 0 66 1335
Size: 66 1269
BsmBI CGTCTCn'nnnn_
Cuts at: 0 433 717 1335
Size: 433 284 618
Fragments arranged by size:
618 433 284
Bsp1286I G_dGCh'C
Cuts at: 0 260 401 434 824 842 1019 1095 1335
Size: 260 141 33 390 18 177 76 240
Fragments arranged by size:
390 260 240 177 141 76 33 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1052 1095 1335
Size: 1052 43 240
Fragments arranged by size:
1052 240 43
BsrBI CCG'CTC
Cuts at: 0 198 1335
Size: 198 1137
BsrDI GCAATG_nn'
Cuts at: 0 506 1335
Size: 506 829
BsrFI r'CCGG_y
Cuts at: 0 306 1335
Size: 306 1029
BssHII G'CGCG_C
Cuts at: 0 556 1335
Size: 556 779
BssSI C'ACGA_G
Cuts at: 0 433 1335
Size: 433 902
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1014 1335
Size: 1014 321
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1200 1307 1335
Size: 1200 107 28
Fragments arranged by size:
1200 107 28
BstYI r'GATC_y
Cuts at: 0 920 1335
Size: 920 415
BstZ17I GTA'TAC
Cuts at: 0 1163 1335
Size: 1163 172
BtgI C'CryG_G
Cuts at: 0 263 608 614 960 1335
Size: 263 345 6 346 375
Fragments arranged by size:
375 346 345 263 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 315 438 1335
Size: 315 123 897
Fragments arranged by size:
897 315 123
EaeI y'GGCC_r
Cuts at: 0 611 815 847 880 942 1335
Size: 611 204 32 33 62 393
Fragments arranged by size:
611 393 204 62 33 32
EagI C'GGCC_G
Cuts at: 0 611 880 1335
Size: 611 269 455
Fragments arranged by size:
611 455 269
EarI CTCTTCn'nnn_
Cuts at: 0 82 406 1335
Size: 82 324 929
Fragments arranged by size:
929 324 82
EcoICRI GAG'CTC
Cuts at: 0 399 822 1335
Size: 399 423 513
Fragments arranged by size:
513 423 399
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 214 655 706 856 1335
Size: 25 189 441 51 150 479
Fragments arranged by size:
479 441 189 150 51 25
HaeII r_GCGC'y
Cuts at: 0 41 125 360 577 1297 1335
Size: 41 84 235 217 720 38
Fragments arranged by size:
720 235 217 84 41 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 220 252 1207 1239 1335
Size: 220 32 955 32 96
Fragments arranged by size:
955 220 96 32 32
KasI G'GCGC_C
Cuts at: 0 1293 1335
Size: 1293 42
MscI TGG'CCA
Cuts at: 0 849 1335
Size: 849 486
MslI CAynn'nnrTG
Cuts at: 0 511 1305 1335
Size: 511 794 30
Fragments arranged by size:
794 511 30
MspA1I CmG'CkG
Cuts at: 0 246 265 569 610 681 891 1029 1050
Size: 246 19 304 41 71 210 138 21
Cuts at: 1050 1101 1335
Size: 51 234
Fragments arranged by size:
304 246 234 210 138 71 51 41
21 19
NaeI GCC'GGC
Cuts at: 0 308 1335
Size: 308 1027
NarI GG'CG_CC
Cuts at: 0 1294 1335
Size: 1294 41
NgoMIV G'CCGG_C
Cuts at: 0 306 1335
Size: 306 1029
PflMI CCAn_nnn'nTGG
Cuts at: 0 100 1335
Size: 100 1235
PstI C_TGCA'G
Cuts at: 0 21 218 1049 1106 1335
Size: 21 197 831 57 229
Fragments arranged by size:
831 229 197 57 21
PvuII CAG'CTG
Cuts at: 0 1029 1101 1335
Size: 1029 72 234
Fragments arranged by size:
1029 234 72
SacI G_AGCT'C
Cuts at: 0 401 824 1335
Size: 401 423 511
Fragments arranged by size:
511 423 401
SacII CC_GC'GG
Cuts at: 0 266 611 1335
Size: 266 345 724
Fragments arranged by size:
724 345 266
SapI GCTCTTCn'nnn_
Cuts at: 0 406 1335
Size: 406 929
ScaI AGT'ACT
Cuts at: 0 661 1335
Size: 661 674
SfcI C'TryA_G
Cuts at: 0 17 214 1045 1102 1335
Size: 17 197 831 57 233
Fragments arranged by size:
831 233 197 57 17
SfoI GGC'GCC
Cuts at: 0 1295 1335
Size: 1295 40
SgrAI Cr'CCGG_yG
Cuts at: 0 306 1335
Size: 306 1029
SmaI CCC'GGG
Cuts at: 0 154 1335
Size: 154 1181
SmlI C'TyrA_G
Cuts at: 0 924 985 1219 1335
Size: 924 61 234 116
Fragments arranged by size:
924 234 116 61
StuI AGG'CCT
Cuts at: 0 762 856 1057 1335
Size: 762 94 201 278
Fragments arranged by size:
762 278 201 94
StyI C'CwwG_G
Cuts at: 0 1193 1335
Size: 1193 142
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1039 1079 1192 1335
Size: 1039 40 113 143
Fragments arranged by size:
1039 143 113 40
TatI w'GTAC_w
Cuts at: 0 659 1335
Size: 659 676
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 829 861 1335
Size: 829 32 474
Fragments arranged by size:
829 474 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 857 1335
Size: 857 478
XmaI C'CCGG_G
Cuts at: 0 152 1335
Size: 152 1183
XmnI GAAnn'nnTTC
Cuts at: 0 161 1335
Size: 161 1174
Enzymes that do cut:
AarI AccI AcuI AfeI AflII AhdI AleI AlfI
AlwNI ApaLI AvaI BanI BanII BbeI BbsI BglI
BglII BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstXI BstYI BstZ17I BtgI BtgZI
EaeI EagI EarI EcoICRI Eco57MI HaeII Hin4I KasI
MscI MslI MspA1I NaeI NarI NgoMIV PflMI PstI
PvuII SacI SacII SapI ScaI SfcI SfoI SgrAI
SmaI SmlI StuI StyI TaqII TatI TstI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflIII AgeI AloI ApaI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI
BciVI BclI BfrBI BmrI BmtI BsaI BsaAI BsiWI
BspEI BspHI BsrGI BstBI BstEII Bsu36I BtsI ClaI
CspCI DraI DraIII DrdI EciI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MmeI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SalI SanDI SbfI SexAI SfiI SgfI
SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI
XhoI ZraI