(Linear) (Six-base) MAPSORT of: KIBB4051.seq  Check: 2367  from: 1  to: 1023



KIBB4051.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:37  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     421     701    1023 
   Size:        421     280     322

  Fragments arranged by size: 

                 421     322     280
 
AflII C'TTAA_G

Cuts at:      0     493    1023 
   Size:        493     530
 
AlwNI CAG_nnn'CTG

Cuts at:      0     598    1023 
   Size:        598     425
 
ApoI r'AATT_y

Cuts at:      0     113    1023 
   Size:        113     910
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     525    1023 
   Size:        525     498
 
BfrBI ATG'CAT

Cuts at:      0     218    1023 
   Size:        218     805
 
BglII A'GATC_T

Cuts at:      0     489    1023 
   Size:        489     534
 
BlpI GC'TnA_GC

Cuts at:      0     442    1023 
   Size:        442     581
 
Bme1580I G_kGCm'C

Cuts at:      0     168     453    1023 
   Size:        168     285     570

  Fragments arranged by size: 

                 570     285     168
 
BsaBI GATnn'nnATC

Cuts at:      0     736    1023 
   Size:        736     287
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     386    1023 
   Size:        386     637
 
BseYI C'CCAG_C

Cuts at:      0    1013    1023 
   Size:       1013      10
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      77    1023 
   Size:         77     946
 
BsmI GAATG_Cn'

Cuts at:      0     220    1023 
   Size:        220     803
 
Bsp1286I G_dGCh'C

Cuts at:      0     168     453    1023 
   Size:        168     285     570

  Fragments arranged by size: 

                 570     285     168
 
BsrDI GCAATG_nn'

Cuts at:      0     752    1023 
   Size:        752     271
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     543    1023 
   Size:        543     480
 
BstYI r'GATC_y

Cuts at:      0     489    1023 
   Size:        489     534
 
Bsu36I CC'TnA_GG

Cuts at:      0     947    1023 
   Size:        947      76
 
BtgI C'CryG_G

Cuts at:      0     255    1023 
   Size:        255     768
 
BtsI GCAGTG_nn'

Cuts at:      0     110     453    1023 
   Size:        110     343     570

  Fragments arranged by size: 

                 570     343     110
 
DraI TTT'AAA

Cuts at:      0    1002    1023 
   Size:       1002      21
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     421     701    1023 
   Size:        421     280     322

  Fragments arranged by size: 

                 421     322     280
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     226    1023 
   Size:        226     797
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     810    1023 
   Size:        810     213
 
HaeII r_GCGC'y

Cuts at:      0     335    1023 
   Size:        335     688
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     174     206    1023 
   Size:        174      32     817

  Fragments arranged by size: 

                 817     174      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     509    1023 
   Size:        509     514
 
MslI CAynn'nnrTG

Cuts at:      0     194     880    1023 
   Size:        194     686     143

  Fragments arranged by size: 

                 686     194     143
 
MspA1I CmG'CkG

Cuts at:      0     441    1013    1023 
   Size:        441     572      10

  Fragments arranged by size: 

                 572     441      10
 
NsiI A_TGCA'T

Cuts at:      0     220    1023 
   Size:        220     803
 
NspI r_CATG'y

Cuts at:      0     656    1023 
   Size:        656     367
 
PfoI T'CCnGG_A

Cuts at:      0     397    1023 
   Size:        397     626
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     539     571     962     994    1023 
   Size:        539      32     391      32      29

  Fragments arranged by size: 

                 539     391      32      32      29
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     573     605    1023 
   Size:        573      32     418

  Fragments arranged by size: 

                 573     418      32
 
PvuII CAG'CTG

Cuts at:      0     441    1013    1023 
   Size:        441     572      10

  Fragments arranged by size: 

                 572     441      10
 
SexAI A'CCwGG_T

Cuts at:      0     523    1023 
   Size:        523     500
 
SmlI C'TyrA_G

Cuts at:      0     493    1023 
   Size:        493     530
 
TatI w'GTAC_w

Cuts at:      0     987    1023 
   Size:        987      36
 

 Enzymes that do cut:

     AcuI    AflII    AlwNI     ApoI    BciVI    BfrBI    BglII     BlpI
 Bme1580I    BsaBI    BseRI    BseYI     BsgI     BsmI Bsp1286I    BsrDI
    BstXI    BstYI   Bsu36I     BtgI     BtsI     DraI  Eco57MI    EcoNI
     FalI    HaeII    Hin4I     MmeI     MslI   MspA1I     NsiI     NspI
     PfoI     PpiI     PsrI    PvuII    SexAI     SmlI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI     BclI     BglI    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI
    BsaXI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I    BtgZI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI EcoO109I    EcoRI    EcoRV     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     PacI     PasI     PciI    PflMI     PmeI     PmlI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PstI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI