Restriction map for
pF1KB4082
(Linear) (Six-base) MAPSORT of: KIBB4082.seq Check: 879 from: 1 to: 2682
KIBB4082.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AatII G_ACGT'C
Cuts at: 0 596 2682
Size: 596 2086
AccI GT'mk_AC
Cuts at: 0 913 1367 2682
Size: 913 454 1315
Fragments arranged by size:
1315 913 454
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 908 2024 2682
Size: 908 1116 658
Fragments arranged by size:
1116 908 658
AfeI AGC'GCT
Cuts at: 0 147 856 2451 2682
Size: 147 709 1595 231
Fragments arranged by size:
1595 709 231 147
AflIII A'CryG_T
Cuts at: 0 1392 2654 2682
Size: 1392 1262 28
Fragments arranged by size:
1392 1262 28
AhdI GACnn_n'nnGTC
Cuts at: 0 321 2682
Size: 321 2361
ApaI G_GGCC'C
Cuts at: 0 357 2682
Size: 357 2325
ApoI r'AATT_y
Cuts at: 0 601 880 1804 2065 2533 2682
Size: 601 279 924 261 468 149
Fragments arranged by size:
924 601 468 279 261 149
AvaI C'yCGr_G
Cuts at: 0 282 2168 2682
Size: 282 1886 514
Fragments arranged by size:
1886 514 282
AvrII C'CTAG_G
Cuts at: 0 705 2682
Size: 705 1977
BamHI G'GATC_C
Cuts at: 0 1449 1644 2682
Size: 1449 195 1038
Fragments arranged by size:
1449 1038 195
BanI G'GyrC_C
Cuts at: 0 1840 2682
Size: 1840 842
BanII G_rGCy'C
Cuts at: 0 357 1071 2682
Size: 357 714 1611
Fragments arranged by size:
1611 714 357
BbsI GAAGACnn'nnnn_
Cuts at: 0 36 445 1757 2012 2682
Size: 36 409 1312 255 670
Fragments arranged by size:
1312 670 409 255 36
BclI T'GATC_A
Cuts at: 0 1925 2682
Size: 1925 757
BfrBI ATG'CAT
Cuts at: 0 783 2682
Size: 783 1899
BlpI GC'TnA_GC
Cuts at: 0 1020 2313 2682
Size: 1020 1293 369
Fragments arranged by size:
1293 1020 369
Bme1580I G_kGCm'C
Cuts at: 0 357 1040 2682
Size: 357 683 1642
Fragments arranged by size:
1642 683 357
BmrI ACTGGGnnnn_n'
Cuts at: 0 270 2682
Size: 270 2412
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1031 1062 2227 2682
Size: 1031 31 1165 455
Fragments arranged by size:
1165 1031 455 31
Bpu10I CC'TnA_GC
Cuts at: 0 2461 2682
Size: 2461 221
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 702 2682
Size: 702 1980
BsaAI yAC'GTr
Cuts at: 0 222 2682
Size: 222 2460
BsaHI Gr'CG_yC
Cuts at: 0 593 2682
Size: 593 2089
BsaWI w'CCGG_w
Cuts at: 0 2050 2682
Size: 2050 632
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 990 1020 1685 1715 2682
Size: 990 30 665 30 967
Fragments arranged by size:
990 967 665 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 987 2682
Size: 987 1695
BseYI C'CCAG_C
Cuts at: 0 426 857 2682
Size: 426 431 1825
Fragments arranged by size:
1825 431 426
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 184 343 424 2682
Size: 154 30 159 81 2258
Fragments arranged by size:
2258 159 154 81 30
BsiHKAI G_wGCw'C
Cuts at: 0 35 143 1071 2682
Size: 35 108 928 1611
Fragments arranged by size:
1611 928 108 35
BsmI GAATG_Cn'
Cuts at: 0 903 2682
Size: 903 1779
Bsp1286I G_dGCh'C
Cuts at: 0 35 143 357 1040 1071 2682
Size: 35 108 214 683 31 1611
Fragments arranged by size:
1611 683 214 108 35 31
BspHI T'CATG_A
Cuts at: 0 119 1574 1595 2682
Size: 119 1455 21 1087
Fragments arranged by size:
1455 1087 119 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1712 2682
Size: 1712 970
BsrDI GCAATG_nn'
Cuts at: 0 1248 2472 2682
Size: 1248 1224 210
Fragments arranged by size:
1248 1224 210
BsrGI T'GTAC_A
Cuts at: 0 1310 2682
Size: 1310 1372
BssSI C'ACGA_G
Cuts at: 0 1769 2682
Size: 1769 913
BstAPI GCAn_nnn'nTGC
Cuts at: 0 30 1030 2682
Size: 30 1000 1652
Fragments arranged by size:
1652 1000 30
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2520 2682
Size: 2520 162
BstYI r'GATC_y
Cuts at: 0 1449 1644 2077 2682
Size: 1449 195 433 605
Fragments arranged by size:
1449 605 433 195
BstZ17I GTA'TAC
Cuts at: 0 914 2682
Size: 914 1768
Bsu36I CC'TnA_GG
Cuts at: 0 970 2682
Size: 970 1712
BtsI GCAGTG_nn'
Cuts at: 0 548 2682
Size: 548 2134
ClaI AT'CG_AT
Cuts at: 0 1148 2682
Size: 1148 1534
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 557 592 2682
Size: 557 35 2090
Fragments arranged by size:
2090 557 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 664 2682
Size: 664 2018
EaeI y'GGCC_r
Cuts at: 0 2 2510 2682
Size: 2 2508 172
Fragments arranged by size:
2508 172 2
EcoICRI GAG'CTC
Cuts at: 0 1069 2682
Size: 1069 1613
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 908 1031 1062 2024 2227 2682
Size: 908 123 31 962 203 455
Fragments arranged by size:
962 908 455 203 123 31
EcoO109I rG'GnC_Cy
Cuts at: 0 353 354 2039 2682
Size: 353 1 1685 643
Fragments arranged by size:
1685 643 353 1
EcoRI G'AATT_C
Cuts at: 0 601 880 2533 2682
Size: 601 279 1653 149
Fragments arranged by size:
1653 601 279 149
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 666 2214 2682
Size: 666 1548 468
Fragments arranged by size:
1548 666 468
HaeII r_GCGC'y
Cuts at: 0 149 443 858 2453 2682
Size: 149 294 415 1595 229
Fragments arranged by size:
1595 415 294 229 149
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 307 339 500 532 1237 1269 1971 2003
Size: 307 32 161 32 705 32 702 32
Cuts at: 2003 2436 2468 2682
Size: 433 32 214
Fragments arranged by size:
705 702 433 307 214 161 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 819 2213 2682
Size: 819 1394 469
Fragments arranged by size:
1394 819 469
HindIII A'AGCT_T
Cuts at: 0 956 2682
Size: 956 1726
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1878 2475 2682
Size: 1878 597 207
Fragments arranged by size:
1878 597 207
MscI TGG'CCA
Cuts at: 0 4 2512 2682
Size: 4 2508 170
Fragments arranged by size:
2508 170 4
MslI CAynn'nnrTG
Cuts at: 0 418 1150 1276 2518 2682
Size: 418 732 126 1242 164
Fragments arranged by size:
1242 732 418 164 126
MspA1I CmG'CkG
Cuts at: 0 2125 2682
Size: 2125 557
NdeI CA'TA_TG
Cuts at: 0 1302 2034 2682
Size: 1302 732 648
Fragments arranged by size:
1302 732 648
NsiI A_TGCA'T
Cuts at: 0 785 2682
Size: 785 1897
NspI r_CATG'y
Cuts at: 0 738 1396 2682
Size: 738 658 1286
Fragments arranged by size:
1286 738 658
PciI A'CATG_T
Cuts at: 0 1392 2682
Size: 1392 1290
PfoI T'CCnGG_A
Cuts at: 0 994 2428 2682
Size: 994 1434 254
Fragments arranged by size:
1434 994 254
PpuMI rG'GwC_Cy
Cuts at: 0 2039 2682
Size: 2039 643
PspOMI G'GGCC_C
Cuts at: 0 353 2682
Size: 353 2329
SacI G_AGCT'C
Cuts at: 0 1071 2682
Size: 1071 1611
SanDI GG'GwC_CC
Cuts at: 0 2039 2682
Size: 2039 643
SmlI C'TyrA_G
Cuts at: 0 282 717 2682
Size: 282 435 1965
Fragments arranged by size:
1965 435 282
SspI AAT'ATT
Cuts at: 0 1107 1814 2682
Size: 1107 707 868
Fragments arranged by size:
1107 868 707
StyI C'CwwG_G
Cuts at: 0 705 1835 2183 2420 2682
Size: 705 1130 348 237 262
Fragments arranged by size:
1130 705 348 262 237
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 849 1828 2602 2682
Size: 849 979 774 80
Fragments arranged by size:
979 849 774 80
TatI w'GTAC_w
Cuts at: 0 1086 1310 2225 2682
Size: 1086 224 915 457
Fragments arranged by size:
1086 915 457 224
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 220 252 990 1022 1425 1457 2682
Size: 220 32 738 32 403 32 1225
Fragments arranged by size:
1225 738 403 220 32 32 32
XhoI C'TCGA_G
Cuts at: 0 282 2682
Size: 282 2400
XmnI GAAnn'nnTTC
Cuts at: 0 202 2682
Size: 202 2480
ZraI GAC'GTC
Cuts at: 0 594 2682
Size: 594 2088
Enzymes that do cut:
AatII AccI AcuI AfeI AflIII AhdI ApaI ApoI
AvaI AvrII BamHI BanI BanII BbsI BclI BfrBI
BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrDI BsrGI BssSI BstAPI BstXI BstYI
BstZ17I Bsu36I BtsI ClaI CspCI DrdI EaeI EcoICRI
Eco57MI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII
MmeI MscI MslI MspA1I NdeI NsiI NspI PciI
PfoI PpuMI PspOMI SacI SanDI SmlI SspI StyI
TaqII TatI TstI XhoI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflII AgeI AleI AlfI AloI
AlwNI ApaLI AscI AseI AsiSI BaeI BbeI BbvCI
BcgI BciVI BglI BglII BmgBI BmtI BplI BsaI
BsaBI BsiEI BsiWI BsmBI BspEI BsrBI BsrFI BssHII
BstBI BstEII BtgI BtgZI DraI DraIII EagI EarI
EciI EcoNI EcoRV FseI FspI FspAI HpaI KasI
KpnI MfeI MluI NaeI NarI NcoI NgoMIV NheI
NotI NruI PacI PasI PflMI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII
SacII SalI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StuI SwaI Tth111I XbaI XcmI XmaI