Restriction map for
pF1KB4084
(Linear) (Six-base) MAPSORT of: KIBB4084.seq Check: 3789 from: 1 to: 2907
KIBB4084.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2823 2907
Size: 2823 84
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1006 1430 2134 2475 2907
Size: 1006 424 704 341 432
Fragments arranged by size:
1006 704 432 424 341
AflIII A'CryG_T
Cuts at: 0 443 1275 2036 2907
Size: 443 832 761 871
Fragments arranged by size:
871 832 761 443
AhdI GACnn_n'nnGTC
Cuts at: 0 285 1537 2907
Size: 285 1252 1370
Fragments arranged by size:
1370 1252 285
AleI CACnn'nnGTG
Cuts at: 0 2780 2907
Size: 2780 127
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 464 958 2907
Size: 464 494 1949
Fragments arranged by size:
1949 494 464
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1265 1297 2907
Size: 1265 32 1610
Fragments arranged by size:
1610 1265 32
AlwNI CAG_nnn'CTG
Cuts at: 0 124 867 2907
Size: 124 743 2040
Fragments arranged by size:
2040 743 124
ApaI G_GGCC'C
Cuts at: 0 816 2804 2907
Size: 816 1988 103
Fragments arranged by size:
1988 816 103
ApaLI G'TGCA_C
Cuts at: 0 80 1620 2215 2392 2907
Size: 80 1540 595 177 515
Fragments arranged by size:
1540 595 515 177 80
ApoI r'AATT_y
Cuts at: 0 1029 2071 2197 2523 2907
Size: 1029 1042 126 326 384
Fragments arranged by size:
1042 1029 384 326 126
AvaI C'yCGr_G
Cuts at: 0 700 742 2546 2907
Size: 700 42 1804 361
Fragments arranged by size:
1804 700 361 42
AvrII C'CTAG_G
Cuts at: 0 253 2907
Size: 253 2654
BamHI G'GATC_C
Cuts at: 0 696 2141 2907
Size: 696 1445 766
Fragments arranged by size:
1445 766 696
BanI G'GyrC_C
Cuts at: 0 224 2907
Size: 224 2683
BanII G_rGCy'C
Cuts at: 0 115 816 2804 2907
Size: 115 701 1988 103
Fragments arranged by size:
1988 701 115 103
BbsI GAAGACnn'nnnn_
Cuts at: 0 473 1189 1272 1854 2895 2907
Size: 473 716 83 582 1041 12
Fragments arranged by size:
1041 716 582 473 83 12
BbvCI CC'TCA_GC
Cuts at: 0 749 2907
Size: 749 2158
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 307 341 1864 1898 2907
Size: 307 34 1523 34 1009
Fragments arranged by size:
1523 1009 307 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2078 2907
Size: 2078 829
BclI T'GATC_A
Cuts at: 0 1497 2907
Size: 1497 1410
BglI GCCn_nnn'nGGC
Cuts at: 0 820 2907
Size: 820 2087
BglII A'GATC_T
Cuts at: 0 1805 2907
Size: 1805 1102
BlpI GC'TnA_GC
Cuts at: 0 1675 2907
Size: 1675 1232
Bme1580I G_kGCm'C
Cuts at: 0 84 816 1331 1624 1850 2219 2389 2396
Size: 84 732 515 293 226 369 170 7
Cuts at: 2396 2463 2804 2907
Size: 67 341 103
Fragments arranged by size:
732 515 369 341 293 226 170 103
84 67 7
BmgBI CAC'GTC
Cuts at: 0 336 1860 2907
Size: 336 1524 1047
Fragments arranged by size:
1524 1047 336
BmrI ACTGGGnnnn_n'
Cuts at: 0 1187 2907
Size: 1187 1720
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 825 1129 2431 2907
Size: 120 705 304 1302 476
Fragments arranged by size:
1302 705 476 304 120
Bpu10I CC'TnA_GC
Cuts at: 0 484 749 2907
Size: 484 265 2158
Fragments arranged by size:
2158 484 265
BsaI GGTCTCn'nnnn_
Cuts at: 0 879 916 1223 2907
Size: 879 37 307 1684
Fragments arranged by size:
1684 879 307 37
BsaAI yAC'GTr
Cuts at: 0 444 1503 2907
Size: 444 1059 1404
Fragments arranged by size:
1404 1059 444
BsaBI GATnn'nnATC
Cuts at: 0 976 2907
Size: 976 1931
BsaHI Gr'CG_yC
Cuts at: 0 1703 2907
Size: 1703 1204
BsaWI w'CCGG_w
Cuts at: 0 881 2907
Size: 881 2026
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 727 826 1075 2907
Size: 727 99 249 1832
Fragments arranged by size:
1832 727 249 99
BseYI C'CCAG_C
Cuts at: 0 328 889 1285 1611 2477 2602 2907
Size: 328 561 396 326 866 125 305
Fragments arranged by size:
866 561 396 328 326 305 125
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 1020 1716 2907
Size: 122 898 696 1191
Fragments arranged by size:
1191 898 696 122
BsiHKAI G_wGCw'C
Cuts at: 0 84 685 836 1624 2219 2396 2907
Size: 84 601 151 788 595 177 511
Fragments arranged by size:
788 601 595 511 177 151 84
BsiWI C'GTAC_G
Cuts at: 0 2052 2907
Size: 2052 855
BsmBI CGTCTCn'nnnn_
Cuts at: 0 293 2566 2907
Size: 293 2273 341
Fragments arranged by size:
2273 341 293
Bsp1286I G_dGCh'C
Cuts at: 0 84 115 685 816 836 1331 1624 1850
Size: 84 31 570 131 20 495 293 226
Cuts at: 1850 2219 2389 2396 2463 2804 2907
Size: 369 170 7 67 341 103
Fragments arranged by size:
570 495 369 341 293 226 170 131
103 84 67 31 20 7
BspEI T'CCGG_A
Cuts at: 0 881 2907
Size: 881 2026
BspHI T'CATG_A
Cuts at: 0 1647 1745 2567 2907
Size: 1647 98 822 340
Fragments arranged by size:
1647 822 340 98
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1217 2480 2823 2907
Size: 1217 1263 343 84
Fragments arranged by size:
1263 1217 343 84
BsrFI r'CCGG_y
Cuts at: 0 191 1171 2907
Size: 191 980 1736
Fragments arranged by size:
1736 980 191
BssHII G'CGCG_C
Cuts at: 0 2838 2907
Size: 2838 69
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2472 2907
Size: 2472 435
BstEII G'GTnAC_C
Cuts at: 0 900 2907
Size: 900 2007
BstXI CCAn_nnnn'nTGG
Cuts at: 0 328 1073 1713 1978 2409 2580 2907
Size: 328 745 640 265 431 171 327
Fragments arranged by size:
745 640 431 328 327 265 171
BstYI r'GATC_y
Cuts at: 0 696 1805 2141 2907
Size: 696 1109 336 766
Fragments arranged by size:
1109 766 696 336
Bsu36I CC'TnA_GG
Cuts at: 0 89 436 2907
Size: 89 347 2471
Fragments arranged by size:
2471 347 89
BtgI C'CryG_G
Cuts at: 0 220 896 1316 2843 2907
Size: 220 676 420 1527 64
Fragments arranged by size:
1527 676 420 220 64
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1242 2907
Size: 1242 1665
BtsI GCAGTG_nn'
Cuts at: 0 82 105 852 2907
Size: 82 23 747 2055
Fragments arranged by size:
2055 747 82 23
ClaI AT'CG_AT
Cuts at: 0 558 2907
Size: 558 2349
DraIII CAC_nnn'GTG
Cuts at: 0 680 2907
Size: 680 2227
EaeI y'GGCC_r
Cuts at: 0 2 21 1075 2907
Size: 2 19 1054 1832
Fragments arranged by size:
1832 1054 19 2
EarI CTCTTCn'nnn_
Cuts at: 0 158 569 877 1375 1478 1622 2899 2907
Size: 158 411 308 498 103 144 1277 8
Fragments arranged by size:
1277 498 411 308 158 144 103 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1353 2907
Size: 1353 1554
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 825 1006 1129 1430 2134 2431 2475
Size: 120 705 181 123 301 704 297 44
Cuts at: 2475 2907
Size: 432
Fragments arranged by size:
705 704 432 301 297 181 123 120
44
EcoNI CCTnn'n_nnAGG
Cuts at: 0 92 742 2907
Size: 92 650 2165
Fragments arranged by size:
2165 650 92
EcoO109I rG'GnC_Cy
Cuts at: 0 340 380 432 813 1791 2801 2886 2907
Size: 340 40 52 381 978 1010 85 21
Fragments arranged by size:
1010 978 381 340 85 52 40 21
EcoRI G'AATT_C
Cuts at: 0 1029 2071 2907
Size: 1029 1042 836
Fragments arranged by size:
1042 1029 836
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 271 303 681 713 1051 1083 1110 1125
Size: 271 32 378 32 338 32 27 15
Cuts at: 1125 1142 1157 1201 1233 1482 1514 1523 1555
Size: 17 15 44 32 249 32 9 32
Cuts at: 1555 1904 1936 2907
Size: 349 32 971
Fragments arranged by size:
971 378 349 338 271 249 44 32
32 32 32 32 32 32 27 17
15 15 9
HincII GTy'rAC
Cuts at: 0 804 1379 1395 1830 2700 2907
Size: 804 575 16 435 870 207
Fragments arranged by size:
870 804 575 435 207 16
HindIII A'AGCT_T
Cuts at: 0 1438 2907
Size: 1438 1469
HpaI GTT'AAC
Cuts at: 0 1379 2907
Size: 1379 1528
MluI A'CGCG_T
Cuts at: 0 1275 2907
Size: 1275 1632
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1119 1941 2907
Size: 1119 822 966
Fragments arranged by size:
1119 966 822
MscI TGG'CCA
Cuts at: 0 4 23 1077 2907
Size: 4 19 1054 1830
Fragments arranged by size:
1830 1054 19 4
MslI CAynn'nnrTG
Cuts at: 0 940 1711 1976 2780 2907
Size: 940 771 265 804 127
Fragments arranged by size:
940 804 771 265 127
MspA1I CmG'CkG
Cuts at: 0 32 77 528 1116 1674 2257 2907
Size: 32 45 451 588 558 583 650
Fragments arranged by size:
650 588 583 558 451 45 32
NaeI GCC'GGC
Cuts at: 0 1173 2907
Size: 1173 1734
NcoI C'CATG_G
Cuts at: 0 1316 2843 2907
Size: 1316 1527 64
Fragments arranged by size:
1527 1316 64
NgoMIV G'CCGG_C
Cuts at: 0 1171 2907
Size: 1171 1736
NspI r_CATG'y
Cuts at: 0 1605 2431 2907
Size: 1605 826 476
Fragments arranged by size:
1605 826 476
PflMI CCAn_nnn'nTGG
Cuts at: 0 194 1317 2907
Size: 194 1123 1590
Fragments arranged by size:
1590 1123 194
PfoI T'CCnGG_A
Cuts at: 0 136 2020 2907
Size: 136 1884 887
Fragments arranged by size:
1884 887 136
PmlI CAC'GTG
Cuts at: 0 444 1503 2907
Size: 444 1059 1404
Fragments arranged by size:
1404 1059 444
PpuMI rG'GwC_Cy
Cuts at: 0 340 380 432 1791 2907
Size: 340 40 52 1359 1116
Fragments arranged by size:
1359 1116 340 52 40
PshAI GACnn'nnGTC
Cuts at: 0 32 338 2907
Size: 32 306 2569
Fragments arranged by size:
2569 306 32
PspOMI G'GGCC_C
Cuts at: 0 812 2800 2907
Size: 812 1988 107
Fragments arranged by size:
1988 812 107
PspXI vC'TCGA_Gb
Cuts at: 0 2546 2907
Size: 2546 361
PstI C_TGCA'G
Cuts at: 0 69 2907
Size: 69 2838
PvuII CAG'CTG
Cuts at: 0 32 77 1116 1674 2907
Size: 32 45 1039 558 1233
Fragments arranged by size:
1233 1039 558 45 32
SanDI GG'GwC_CC
Cuts at: 0 1791 2907
Size: 1791 1116
SapI GCTCTTCn'nnn_
Cuts at: 0 569 877 1478 2899 2907
Size: 569 308 601 1421 8
Fragments arranged by size:
1421 601 569 308 8
ScaI AGT'ACT
Cuts at: 0 2611 2907
Size: 2611 296
SfcI C'TryA_G
Cuts at: 0 65 2736 2907
Size: 65 2671 171
Fragments arranged by size:
2671 171 65
SmlI C'TyrA_G
Cuts at: 0 2546 2907
Size: 2546 361
StuI AGG'CCT
Cuts at: 0 252 2408 2907
Size: 252 2156 499
Fragments arranged by size:
2156 499 252
StyI C'CwwG_G
Cuts at: 0 253 538 1316 1527 2843 2907
Size: 253 285 778 211 1316 64
Fragments arranged by size:
1316 778 285 253 211 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1870 2202 2907
Size: 1870 332 705
Fragments arranged by size:
1870 705 332
TatI w'GTAC_w
Cuts at: 0 929 2375 2609 2907
Size: 929 1446 234 298
Fragments arranged by size:
1446 929 298 234
Tth111I GACn'n_nGTC
Cuts at: 0 2704 2907
Size: 2704 203
XbaI T'CTAG_A
Cuts at: 0 1455 2907
Size: 1455 1452
XhoI C'TCGA_G
Cuts at: 0 2546 2907
Size: 2546 361
XmnI GAAnn'nnTTC
Cuts at: 0 1369 2907
Size: 1369 1538
Enzymes that do cut:
AarI AcuI AflIII AhdI AleI AlfI AloI AlwNI
ApaI ApaLI ApoI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsiWI BsmBI
Bsp1286I BspEI BspHI BspMI BsrFI BssHII BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraIII
EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI Hin4I
HincII HindIII HpaI MluI MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV NspI PflMI PfoI PmlI PpuMI
PshAI PspOMI PspXI PstI PvuII SanDI SapI ScaI
SfcI SmlI StuI StyI TaqII TatI Tth111I XbaI
XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AscI
AseI AsiSI BaeI BbeI BfrBI BmtI BplI BpuEI
BsaXI BsiEI BsmI BsrBI BsrDI BsrGI BssSI BstBI
BstZ17I CspCI DraI DrdI EagI EcoICRI EcoRV FalI
FseI FspI FspAI HaeII KasI KpnI MfeI NarI
NdeI NheI NotI NruI NsiI PacI PasI PciI
PmeI PpiI PsiI PsrI PvuI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TstI XcmI
XmaI ZraI