Restriction map for
pF1KB4095
(Linear) (Six-base) MAPSORT of: KIBB4095.seq Check: 1120 from: 1 to: 3249
KIBB4095.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2212 3249
Size: 2212 1037
AccI GT'mk_AC
Cuts at: 0 77 3249
Size: 77 3172
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 922 1540 3055 3249
Size: 922 618 1515 194
Fragments arranged by size:
1515 922 618 194
AflIII A'CryG_T
Cuts at: 0 1364 1673 3249
Size: 1364 309 1576
Fragments arranged by size:
1576 1364 309
AhdI GACnn_n'nnGTC
Cuts at: 0 2583 3249
Size: 2583 666
AleI CACnn'nnGTG
Cuts at: 0 2012 2242 3249
Size: 2012 230 1007
Fragments arranged by size:
2012 1007 230
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2020 2030 3249
Size: 2020 10 1219
Fragments arranged by size:
2020 1219 10
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 880 912 2538 2570 3249
Size: 880 32 1626 32 679
Fragments arranged by size:
1626 880 679 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 495 1002 1668 2921 3249
Size: 495 507 666 1253 328
Fragments arranged by size:
1253 666 507 495 328
ApaI G_GGCC'C
Cuts at: 0 2135 3249
Size: 2135 1114
ApoI r'AATT_y
Cuts at: 0 1479 3249
Size: 1479 1770
AvaI C'yCGr_G
Cuts at: 0 1532 2259 2820 3249
Size: 1532 727 561 429
Fragments arranged by size:
1532 727 561 429
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2960 2993 3249
Size: 2960 33 256
Fragments arranged by size:
2960 256 33
BamHI G'GATC_C
Cuts at: 0 513 1614 3249
Size: 513 1101 1635
Fragments arranged by size:
1635 1101 513
BanI G'GyrC_C
Cuts at: 0 636 750 2255 2730 3249
Size: 636 114 1505 475 519
Fragments arranged by size:
1505 636 519 475 114
BanII G_rGCy'C
Cuts at: 0 354 470 881 1286 1933 2135 2251 2519
Size: 354 116 411 405 647 202 116 268
Cuts at: 2519 2827 2859 3249
Size: 308 32 390
Fragments arranged by size:
647 411 405 390 354 308 268 202
116 116 32
BbeI G_GCGC'C
Cuts at: 0 2259 2734 3249
Size: 2259 475 515
Fragments arranged by size:
2259 515 475
BbvCI CC'TCA_GC
Cuts at: 0 1309 2483 3249
Size: 1309 1174 766
Fragments arranged by size:
1309 1174 766
BciVI GTATCCnnnnn_n'
Cuts at: 0 2961 3249
Size: 2961 288
BclI T'GATC_A
Cuts at: 0 1130 3249
Size: 1130 2119
BfrBI ATG'CAT
Cuts at: 0 1512 3249
Size: 1512 1737
BglI GCCn_nnn'nGGC
Cuts at: 0 218 1094 2051 2264 2744 2806 2831 3249
Size: 218 876 957 213 480 62 25 418
Fragments arranged by size:
957 876 480 418 218 213 62 25
Bme1580I G_kGCm'C
Cuts at: 0 755 2135 3249
Size: 755 1380 1114
Fragments arranged by size:
1380 1114 755
BmgBI CAC'GTC
Cuts at: 0 774 3249
Size: 774 2475
BmrI ACTGGGnnnn_n'
Cuts at: 0 406 2006 2931 3249
Size: 406 1600 925 318
Fragments arranged by size:
1600 925 406 318
BmtI G_CTAG'C
Cuts at: 0 2813 3249
Size: 2813 436
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 324 2214 2928 3249
Size: 324 1890 714 321
Fragments arranged by size:
1890 714 324 321
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 393 1222 2039 2350 2873 2898 3097 3249
Size: 393 829 817 311 523 25 199 152
Fragments arranged by size:
829 817 523 393 311 199 152 25
Bpu10I CC'TnA_GC
Cuts at: 0 860 1309 2483 2895 3249
Size: 860 449 1174 412 354
Fragments arranged by size:
1174 860 449 412 354
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 814 1342 3190 3249
Size: 814 528 1848 59
Fragments arranged by size:
1848 814 528 59
BsaI GGTCTCn'nnnn_
Cuts at: 0 1648 3249
Size: 1648 1601
BsaAI yAC'GTr
Cuts at: 0 666 690 873 1419 3249
Size: 666 24 183 546 1830
Fragments arranged by size:
1830 666 546 183 24
BsaBI GATnn'nnATC
Cuts at: 0 338 518 1333 3249
Size: 338 180 815 1916
Fragments arranged by size:
1916 815 338 180
BsaHI Gr'CG_yC
Cuts at: 0 2256 2290 2311 2404 2620 2629 2731 3146
Size: 2256 34 21 93 216 9 102 415
Cuts at: 3146 3249
Size: 103
Fragments arranged by size:
2256 415 216 103 102 93 34 21
9
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 431 461 880 910 3249
Size: 431 30 419 30 2339
Fragments arranged by size:
2339 431 419 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 46 49 79 151 154 163 166 187
Size: 46 3 30 72 3 9 3 21
Cuts at: 187 1489 3249
Size: 1302 1760
Fragments arranged by size:
1760 1302 72 46 30 21 9 3
3 3
BseYI C'CCAG_C
Cuts at: 0 485 880 1377 1924 2698 2704 2739 2766
Size: 485 395 497 547 774 6 35 27
Cuts at: 2766 2815 3249
Size: 49 434
Fragments arranged by size:
774 547 497 485 434 395 49 35
27 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 1627 2179 3249
Size: 116 1511 552 1070
Fragments arranged by size:
1511 1070 552 116
BsiEI CG_ry'CG
Cuts at: 0 930 3249
Size: 930 2319
BsiHKAI G_wGCw'C
Cuts at: 0 470 779 1222 1241 1286 1529 2859 3025
Size: 470 309 443 19 45 243 1330 166
Cuts at: 3025 3074 3249
Size: 49 175
Fragments arranged by size:
1330 470 443 309 243 175 166 49
45 19
BsmBI CGTCTCn'nnnn_
Cuts at: 0 436 3249
Size: 436 2813
Bsp1286I G_dGCh'C
Cuts at: 0 354 470 755 779 881 1222 1241 1286
Size: 354 116 285 24 102 341 19 45
Cuts at: 1286 1529 1933 2135 2251 2519 2827 2859 3025
Size: 243 404 202 116 268 308 32 166
Cuts at: 3025 3074 3249
Size: 49 175
Fragments arranged by size:
404 354 341 308 285 268 243 202
175 166 116 116 102 49 45 32
24 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 728 1301 1547 2212 3249
Size: 728 573 246 665 1037
Fragments arranged by size:
1037 728 665 573 246
BsrBI CCG'CTC
Cuts at: 0 87 1233 3249
Size: 87 1146 2016
Fragments arranged by size:
2016 1146 87
BsrDI GCAATG_nn'
Cuts at: 0 587 854 1860 3249
Size: 587 267 1006 1389
Fragments arranged by size:
1389 1006 587 267
BsrFI r'CCGG_y
Cuts at: 0 2631 3249
Size: 2631 618
BsrGI T'GTAC_A
Cuts at: 0 2639 3249
Size: 2639 610
BstAPI GCAn_nnn'nTGC
Cuts at: 0 495 750 3249
Size: 495 255 2499
Fragments arranged by size:
2499 495 255
BstEII G'GTnAC_C
Cuts at: 0 2925 3249
Size: 2925 324
BstXI CCAn_nnnn'nTGG
Cuts at: 0 818 2220 2566 3249
Size: 818 1402 346 683
Fragments arranged by size:
1402 818 683 346
BstYI r'GATC_y
Cuts at: 0 457 513 1304 1614 3209 3249
Size: 457 56 791 310 1595 40
Fragments arranged by size:
1595 791 457 310 56 40
Bsu36I CC'TnA_GG
Cuts at: 0 125 3249
Size: 125 3124
BtgI C'CryG_G
Cuts at: 0 213 560 1961 2177 2240 2795 3140 3249
Size: 213 347 1401 216 63 555 345 109
Fragments arranged by size:
1401 555 347 345 216 213 109 63
BtsI GCAGTG_nn'
Cuts at: 0 60 111 2064 3009 3249
Size: 60 51 1953 945 240
Fragments arranged by size:
1953 945 240 60 51
ClaI AT'CG_AT
Cuts at: 0 317 3249
Size: 317 2932
DraIII CAC_nnn'GTG
Cuts at: 0 1917 2727 3249
Size: 1917 810 522
Fragments arranged by size:
1917 810 522
EaeI y'GGCC_r
Cuts at: 0 563 797 1434 2106 2633 3249
Size: 563 234 637 672 527 616
Fragments arranged by size:
672 637 616 563 527 234
EarI CTCTTCn'nnn_
Cuts at: 0 77 113 128 140 164 1843 3249
Size: 77 36 15 12 24 1679 1406
Fragments arranged by size:
1679 1406 77 36 24 15 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 105 814 3249
Size: 105 709 2435
Fragments arranged by size:
2435 709 105
EcoICRI GAG'CTC
Cuts at: 0 468 1284 2857 3249
Size: 468 816 1573 392
Fragments arranged by size:
1573 816 468 392
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 393 922 1222 1540 2039 2350 2873 2898
Size: 393 529 300 318 499 311 523 25
Cuts at: 2898 3055 3097 3249
Size: 157 42 152
Fragments arranged by size:
529 523 499 393 318 311 300 157
152 42 25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2283 3124 3249
Size: 2283 841 125
Fragments arranged by size:
2283 841 125
EcoO109I rG'GnC_Cy
Cuts at: 0 1025 1353 1536 2131 2132 2444 2451 3249
Size: 1025 328 183 595 1 312 7 798
Fragments arranged by size:
1025 798 595 328 312 183 7 1
FspI TGC'GCA
Cuts at: 0 580 2119 3249
Size: 580 1539 1130
Fragments arranged by size:
1539 1130 580
FspAI rTGC'GCAy
Cuts at: 0 2119 3249
Size: 2119 1130
HaeII r_GCGC'y
Cuts at: 0 2259 2734 3249
Size: 2259 475 515
Fragments arranged by size:
2259 515 475
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 454 486 1030 1062 1546 1578 1818 1850
Size: 454 32 544 32 484 32 240 32
Cuts at: 1850 2569 2601 2641 2673 3249
Size: 719 32 40 32 576
Fragments arranged by size:
719 576 544 484 454 240 40 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 246 2598 3249
Size: 246 2352 651
Fragments arranged by size:
2352 651 246
HindIII A'AGCT_T
Cuts at: 0 1845 3249
Size: 1845 1404
KasI G'GCGC_C
Cuts at: 0 2255 2730 3249
Size: 2255 475 519
Fragments arranged by size:
2255 519 475
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1272 1349 3249
Size: 1272 77 1900
Fragments arranged by size:
1900 1272 77
MscI TGG'CCA
Cuts at: 0 799 3249
Size: 799 2450
MslI CAynn'nnrTG
Cuts at: 0 664 1336 1771 2012 2152 2218 2242 2941
Size: 664 672 435 241 140 66 24 699
Cuts at: 2941 3249
Size: 308
Fragments arranged by size:
699 672 664 435 308 241 140 66
24
MspA1I CmG'CkG
Cuts at: 0 100 495 1611 2232 3249
Size: 100 395 1116 621 1017
Fragments arranged by size:
1116 1017 621 395 100
NaeI GCC'GGC
Cuts at: 0 2633 3249
Size: 2633 616
NarI GG'CG_CC
Cuts at: 0 2256 2731 3249
Size: 2256 475 518
Fragments arranged by size:
2256 518 475
NcoI C'CATG_G
Cuts at: 0 213 2795 3249
Size: 213 2582 454
Fragments arranged by size:
2582 454 213
NgoMIV G'CCGG_C
Cuts at: 0 2631 3249
Size: 2631 618
NheI G'CTAG_C
Cuts at: 0 2809 3249
Size: 2809 440
NsiI A_TGCA'T
Cuts at: 0 1514 3249
Size: 1514 1735
NspI r_CATG'y
Cuts at: 0 649 1368 1677 1893 3021 3249
Size: 649 719 309 216 1128 228
Fragments arranged by size:
1128 719 649 309 228 216
PasI CC'CwG_GG
Cuts at: 0 1029 2127 3249
Size: 1029 1098 1122
Fragments arranged by size:
1122 1098 1029
PciI A'CATG_T
Cuts at: 0 1364 1673 3249
Size: 1364 309 1576
Fragments arranged by size:
1576 1364 309
PflMI CCAn_nnn'nTGG
Cuts at: 0 524 747 1626 2727 2921 3249
Size: 524 223 879 1101 194 328
Fragments arranged by size:
1101 879 524 328 223 194
PfoI T'CCnGG_A
Cuts at: 0 329 2366 3239 3249
Size: 329 2037 873 10
Fragments arranged by size:
2037 873 329 10
PmlI CAC'GTG
Cuts at: 0 690 1419 3249
Size: 690 729 1830
Fragments arranged by size:
1830 729 690
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 880 912 3249
Size: 880 32 2337
Fragments arranged by size:
2337 880 32
PpuMI rG'GwC_Cy
Cuts at: 0 1025 1353 1536 2451 3249
Size: 1025 328 183 915 798
Fragments arranged by size:
1025 915 798 328 183
PshAI GACnn'nnGTC
Cuts at: 0 2992 3249
Size: 2992 257
PspOMI G'GGCC_C
Cuts at: 0 2131 3249
Size: 2131 1118
PstI C_TGCA'G
Cuts at: 0 620 1298 1544 3249
Size: 620 678 246 1705
Fragments arranged by size:
1705 678 620 246
PvuI CG_AT'CG
Cuts at: 0 930 3249
Size: 930 2319
PvuII CAG'CTG
Cuts at: 0 495 1611 3249
Size: 495 1116 1638
Fragments arranged by size:
1638 1116 495
SacI G_AGCT'C
Cuts at: 0 470 1286 2859 3249
Size: 470 816 1573 390
Fragments arranged by size:
1573 816 470 390
SanDI GG'GwC_CC
Cuts at: 0 2451 3249
Size: 2451 798
SapI GCTCTTCn'nnn_
Cuts at: 0 77 113 3249
Size: 77 36 3136
Fragments arranged by size:
3136 77 36
SbfI CC_TGCA'GG
Cuts at: 0 1298 3249
Size: 1298 1951
SexAI A'CCwGG_T
Cuts at: 0 1913 2723 3249
Size: 1913 810 526
Fragments arranged by size:
1913 810 526
SfcI C'TryA_G
Cuts at: 0 616 1294 1540 2835 3249
Size: 616 678 246 1295 414
Fragments arranged by size:
1295 678 616 414 246
SfoI GGC'GCC
Cuts at: 0 2257 2732 3249
Size: 2257 475 517
Fragments arranged by size:
2257 517 475
SmaI CCC'GGG
Cuts at: 0 1534 2261 3249
Size: 1534 727 988
Fragments arranged by size:
1534 988 727
SmlI C'TyrA_G
Cuts at: 0 829 1357 3205 3249
Size: 829 528 1848 44
Fragments arranged by size:
1848 829 528 44
SrfI GCCC'GGGC
Cuts at: 0 2261 3249
Size: 2261 988
StuI AGG'CCT
Cuts at: 0 673 1457 2536 3249
Size: 673 784 1079 713
Fragments arranged by size:
1079 784 713 673
StyI C'CwwG_G
Cuts at: 0 213 1745 2795 3249
Size: 213 1532 1050 454
Fragments arranged by size:
1532 1050 454 213
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 206 499 1618 1954 2355 3249
Size: 206 293 1119 336 401 894
Fragments arranged by size:
1119 894 401 336 293 206
TatI w'GTAC_w
Cuts at: 0 2639 3249
Size: 2639 610
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 431 463 3249
Size: 431 32 2786
Fragments arranged by size:
2786 431 32
XbaI T'CTAG_A
Cuts at: 0 297 3249
Size: 297 2952
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 277 432 708 1959 2184 3072 3249
Size: 277 155 276 1251 225 888 177
Fragments arranged by size:
1251 888 277 276 225 177 155
XmaI C'CCGG_G
Cuts at: 0 1532 2259 3249
Size: 1532 727 990
Fragments arranged by size:
1532 990 727
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AleI AlfI AloI
AlwNI ApaI ApoI AvaI BaeI BamHI BanI BanII
BbeI BbvCI BciVI BclI BfrBI BglI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtsI ClaI DraIII
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI
FspAI HaeII Hin4I HincII HindIII KasI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NsiI
NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI
PshAI PspOMI PstI PvuI PvuII SacI SanDI SapI
SbfI SexAI SfcI SfoI SmaI SmlI SrfI StuI
StyI TaqII TatI TstI XbaI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI ApaLI AscI
AseI AsiSI AvrII BbsI BcgI BglII BlpI BsaWI
BsiWI BsmI BspEI BspHI BssHII BssSI BstBI BstZ17I
BtgZI CspCI DraI DrdI EagI EcoRI EcoRV FalI
FseI HpaI KpnI MfeI MluI NdeI NotI NruI
PacI PmeI PsiI PspXI PsrI RsrII SacII SalI
ScaI SfiI SgfI SgrAI SnaBI SpeI SphI SspI
SwaI Tth111I XhoI XmnI ZraI