Restriction map for
pF1KB4099
(Linear) (Six-base) MAPSORT of: KIBB4099.seq Check: 6213 from: 1 to: 1356
KIBB4099.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1185 1356
Size: 1185 171
AclI AA'CG_TT
Cuts at: 0 215 1356
Size: 215 1141
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 116 659 1356
Size: 48 68 543 697
Fragments arranged by size:
697 543 68 48
AhdI GACnn_n'nnGTC
Cuts at: 0 96 1356
Size: 96 1260
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1006 1038 1356
Size: 1006 32 318
Fragments arranged by size:
1006 318 32
AlwNI CAG_nnn'CTG
Cuts at: 0 850 1356
Size: 850 506
ApoI r'AATT_y
Cuts at: 0 192 1356
Size: 192 1164
AvaI C'yCGr_G
Cuts at: 0 1265 1356
Size: 1265 91
BanI G'GyrC_C
Cuts at: 0 415 1356
Size: 415 941
BbsI GAAGACnn'nnnn_
Cuts at: 0 1065 1091 1356
Size: 1065 26 265
Fragments arranged by size:
1065 265 26
BmrI ACTGGGnnnn_n'
Cuts at: 0 651 874 1356
Size: 651 223 482
Fragments arranged by size:
651 482 223
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 191 589 894 1008 1356
Size: 78 113 398 305 114 348
Fragments arranged by size:
398 348 305 114 113 78
BsaI GGTCTCn'nnnn_
Cuts at: 0 936 1356
Size: 936 420
BsaBI GATnn'nnATC
Cuts at: 0 784 952 1356
Size: 784 168 404
Fragments arranged by size:
784 404 168
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 76 106 111 141 1006 1036 1356
Size: 76 30 5 30 865 30 320
Fragments arranged by size:
865 320 76 30 30 30 5
BsiHKAI G_wGCw'C
Cuts at: 0 482 1356
Size: 482 874
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1026 1356
Size: 1026 330
Bsp1286I G_dGCh'C
Cuts at: 0 482 1356
Size: 482 874
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 733 1185 1356
Size: 733 452 171
Fragments arranged by size:
733 452 171
BsrFI r'CCGG_y
Cuts at: 0 5 1356
Size: 5 1351
BsrGI T'GTAC_A
Cuts at: 0 556 694 782 1356
Size: 556 138 88 574
Fragments arranged by size:
574 556 138 88
BssSI C'ACGA_G
Cuts at: 0 1094 1356
Size: 1094 262
BstAPI GCAn_nnn'nTGC
Cuts at: 0 850 1356
Size: 850 506
BstBI TT'CG_AA
Cuts at: 0 463 1356
Size: 463 893
BtgI C'CryG_G
Cuts at: 0 266 518 1356
Size: 266 252 838
Fragments arranged by size:
838 266 252
BtsI GCAGTG_nn'
Cuts at: 0 1134 1356
Size: 1134 222
DraI TTT'AAA
Cuts at: 0 198 1356
Size: 198 1158
EarI CTCTTCn'nnn_
Cuts at: 0 668 1060 1356
Size: 668 392 296
Fragments arranged by size:
668 392 296
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 78 116 191 589 659 894 1008
Size: 48 30 38 75 398 70 235 114
Cuts at: 1008 1356
Size: 348
Fragments arranged by size:
398 348 235 114 75 70 48 38
30
EcoO109I rG'GnC_Cy
Cuts at: 0 202 1356
Size: 202 1154
EcoRV GAT'ATC
Cuts at: 0 1262 1356
Size: 1262 94
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 76 82 108 111 114 143 1202 1234
Size: 76 6 26 3 3 29 1059 32
Cuts at: 1234 1246 1278 1356
Size: 12 32 78
Fragments arranged by size:
1059 78 76 32 32 29 26 12
6 3 3
MslI CAynn'nnrTG
Cuts at: 0 958 1340 1356
Size: 958 382 16
Fragments arranged by size:
958 382 16
MspA1I CmG'CkG
Cuts at: 0 567 1009 1162 1356
Size: 567 442 153 194
Fragments arranged by size:
567 442 194 153
NcoI C'CATG_G
Cuts at: 0 266 518 1356
Size: 266 252 838
Fragments arranged by size:
838 266 252
PflMI CCAn_nnn'nTGG
Cuts at: 0 1207 1356
Size: 1207 149
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1006 1038 1356
Size: 1006 32 318
Fragments arranged by size:
1006 318 32
PpuMI rG'GwC_Cy
Cuts at: 0 202 1356
Size: 202 1154
PvuII CAG'CTG
Cuts at: 0 567 1162 1356
Size: 567 595 194
Fragments arranged by size:
595 567 194
SfcI C'TryA_G
Cuts at: 0 483 1356
Size: 483 873
SmlI C'TyrA_G
Cuts at: 0 1265 1356
Size: 1265 91
SspI AAT'ATT
Cuts at: 0 228 285 1356
Size: 228 57 1071
Fragments arranged by size:
1071 228 57
StyI C'CwwG_G
Cuts at: 0 266 518 1356
Size: 266 252 838
Fragments arranged by size:
838 266 252
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 910 1356
Size: 910 446
TatI w'GTAC_w
Cuts at: 0 556 694 782 1356
Size: 556 138 88 574
Fragments arranged by size:
574 556 138 88
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 861 1150 1356
Size: 861 289 206
Fragments arranged by size:
861 289 206
XhoI C'TCGA_G
Cuts at: 0 1265 1356
Size: 1265 91
Enzymes that do cut:
AarI AclI AcuI AhdI AloI AlwNI ApoI AvaI
BanI BbsI BmrI BpmI BsaI BsaBI BsaXI BsiHKAI
BsmBI Bsp1286I BspMI BsrFI BsrGI BssSI BstAPI BstBI
BtgI BtsI DraI EarI Eco57MI EcoO109I EcoRV Hin4I
MslI MspA1I NcoI PflMI PpiI PpuMI PvuII SfcI
SmlI SspI StyI TaqII TatI XcmI XhoI
Enzymes that do not cut:
AatII AccI Acc65I AfeI AflII AflIII AgeI AleI
AlfI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BanII BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmtI BplI Bpu10I
BpuEI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiWI BsmI BspEI BspHI BsrBI BsrDI BssHII BstEII
BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraIII
DrdI EaeI EagI EciI EcoICRI EcoNI EcoRI FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PfoI PmeI PmlI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI SwaI TstI
Tth111I XbaI XmaI XmnI ZraI