Restriction map for
pF1KB4107
(Linear) (Six-base) MAPSORT of: KIBB4107.seq Check: 5544 from: 1 to: 1923
KIBB4107.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1257 1923
Size: 1257 666
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1112 1923
Size: 1112 811
AflIII A'CryG_T
Cuts at: 0 698 1923
Size: 698 1225
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 551 583 1923
Size: 551 32 1340
Fragments arranged by size:
1340 551 32
ApaI G_GGCC'C
Cuts at: 0 1358 1923
Size: 1358 565
ApaLI G'TGCA_C
Cuts at: 0 865 1923
Size: 865 1058
ApoI r'AATT_y
Cuts at: 0 37 859 1051 1923
Size: 37 822 192 872
Fragments arranged by size:
872 822 192 37
AvaI C'yCGr_G
Cuts at: 0 530 1923
Size: 530 1393
BamHI G'GATC_C
Cuts at: 0 144 1923
Size: 144 1779
BanI G'GyrC_C
Cuts at: 0 478 1533 1923
Size: 478 1055 390
Fragments arranged by size:
1055 478 390
BanII G_rGCy'C
Cuts at: 0 357 1314 1358 1923
Size: 357 957 44 565
Fragments arranged by size:
957 565 357 44
BbsI GAAGACnn'nnnn_
Cuts at: 0 701 1923
Size: 701 1222
BbvCI CC'TCA_GC
Cuts at: 0 510 1923
Size: 510 1413
BglI GCCn_nnn'nGGC
Cuts at: 0 1272 1923
Size: 1272 651
BglII A'GATC_T
Cuts at: 0 10 669 1923
Size: 10 659 1254
Fragments arranged by size:
1254 659 10
BlpI GC'TnA_GC
Cuts at: 0 392 1923
Size: 392 1531
Bme1580I G_kGCm'C
Cuts at: 0 483 869 1358 1608 1739 1923
Size: 483 386 489 250 131 184
Fragments arranged by size:
489 483 386 250 184 131
BmrI ACTGGGnnnn_n'
Cuts at: 0 193 1923
Size: 193 1730
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1289 1361 1409 1472 1923
Size: 1289 72 48 63 451
Fragments arranged by size:
1289 451 72 63 48
Bpu10I CC'TnA_GC
Cuts at: 0 510 1923
Size: 510 1413
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 611 1923
Size: 611 1312
BsaI GGTCTCn'nnnn_
Cuts at: 0 1320 1923
Size: 1320 603
BsaBI GATnn'nnATC
Cuts at: 0 15 770 1923
Size: 15 755 1153
Fragments arranged by size:
1153 755 15
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1767 1797 1923
Size: 1767 30 126
Fragments arranged by size:
1767 126 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 611 614 974 1045 1304 1439 1923
Size: 611 3 360 71 259 135 484
Fragments arranged by size:
611 484 360 259 135 71 3
BseYI C'CCAG_C
Cuts at: 0 482 722 1923
Size: 482 240 1201
Fragments arranged by size:
1201 482 240
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 655 1466 1670 1727 1923
Size: 655 811 204 57 196
Fragments arranged by size:
811 655 204 196 57
BsiHKAI G_wGCw'C
Cuts at: 0 869 1314 1923
Size: 869 445 609
Fragments arranged by size:
869 609 445
BsmI GAATG_Cn'
Cuts at: 0 393 1343 1923
Size: 393 950 580
Fragments arranged by size:
950 580 393
Bsp1286I G_dGCh'C
Cuts at: 0 357 483 869 1314 1358 1608 1739 1923
Size: 357 126 386 445 44 250 131 184
Fragments arranged by size:
445 386 357 250 184 131 126 44
BspHI T'CATG_A
Cuts at: 0 732 1898 1923
Size: 732 1166 25
Fragments arranged by size:
1166 732 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1003 1257 1557 1923
Size: 1003 254 300 366
Fragments arranged by size:
1003 366 300 254
BssSI C'ACGA_G
Cuts at: 0 1451 1923
Size: 1451 472
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1141 1923
Size: 1141 782
BstYI r'GATC_y
Cuts at: 0 10 144 669 1923
Size: 10 134 525 1254
Fragments arranged by size:
1254 525 134 10
Bsu36I CC'TnA_GG
Cuts at: 0 776 1028 1923
Size: 776 252 895
Fragments arranged by size:
895 776 252
BtsI GCAGTG_nn'
Cuts at: 0 1151 1739 1923
Size: 1151 588 184
Fragments arranged by size:
1151 588 184
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1598 1633 1923
Size: 1598 35 290
Fragments arranged by size:
1598 290 35
DraI TTT'AAA
Cuts at: 0 134 1923
Size: 134 1789
EaeI y'GGCC_r
Cuts at: 0 645 1923
Size: 645 1278
EarI CTCTTCn'nnn_
Cuts at: 0 73 343 557 992 1923
Size: 73 270 214 435 931
Fragments arranged by size:
931 435 270 214 73
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1166 1651 1923
Size: 1166 485 272
Fragments arranged by size:
1166 485 272
EcoICRI GAG'CTC
Cuts at: 0 1312 1923
Size: 1312 611
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1112 1289 1361 1409 1472 1923
Size: 1112 177 72 48 63 451
Fragments arranged by size:
1112 451 177 72 63 48
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1412 1923
Size: 1412 511
EcoO109I rG'GnC_Cy
Cuts at: 0 1354 1355 1923
Size: 1354 1 568
Fragments arranged by size:
1354 568 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 129 161 1132 1164 1692 1724 1740 1772
Size: 129 32 971 32 528 32 16 32
Cuts at: 1772 1923
Size: 151
Fragments arranged by size:
971 528 151 129 32 32 32 32
16
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 470 550 1923
Size: 470 80 1373
Fragments arranged by size:
1373 470 80
MslI CAynn'nnrTG
Cuts at: 0 737 1139 1923
Size: 737 402 784
Fragments arranged by size:
784 737 402
NspI r_CATG'y
Cuts at: 0 400 439 702 1923
Size: 400 39 263 1221
Fragments arranged by size:
1221 400 263 39
PasI CC'CwG_GG
Cuts at: 0 1558 1591 1923
Size: 1558 33 332
Fragments arranged by size:
1558 332 33
PciI A'CATG_T
Cuts at: 0 698 1923
Size: 698 1225
PflMI CCAn_nnn'nTGG
Cuts at: 0 475 1237 1591 1923
Size: 475 762 354 332
Fragments arranged by size:
762 475 354 332
PfoI T'CCnGG_A
Cuts at: 0 458 1305 1377 1923
Size: 458 847 72 546
Fragments arranged by size:
847 546 458 72
PspOMI G'GGCC_C
Cuts at: 0 1354 1923
Size: 1354 569
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 260 292 1923
Size: 260 32 1631
Fragments arranged by size:
1631 260 32
PstI C_TGCA'G
Cuts at: 0 129 1923
Size: 129 1794
SacI G_AGCT'C
Cuts at: 0 1314 1923
Size: 1314 609
ScaI AGT'ACT
Cuts at: 0 230 810 1923
Size: 230 580 1113
Fragments arranged by size:
1113 580 230
SfcI C'TryA_G
Cuts at: 0 125 1280 1923
Size: 125 1155 643
Fragments arranged by size:
1155 643 125
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1272 1923
Size: 1272 651
SmlI C'TyrA_G
Cuts at: 0 626 1923
Size: 626 1297
SspI AAT'ATT
Cuts at: 0 370 1923
Size: 370 1553
StuI AGG'CCT
Cuts at: 0 1286 1923
Size: 1286 637
StyI C'CwwG_G
Cuts at: 0 1465 1542 1631 1712 1923
Size: 1465 77 89 81 211
Fragments arranged by size:
1465 211 89 81 77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1626 1851 1923
Size: 1626 225 72
Fragments arranged by size:
1626 225 72
TatI w'GTAC_w
Cuts at: 0 228 808 1866 1923
Size: 228 580 1058 57
Fragments arranged by size:
1058 580 228 57
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 479 511 1923
Size: 479 32 1412
Fragments arranged by size:
1412 479 32
XmnI GAAnn'nnTTC
Cuts at: 0 272 1923
Size: 272 1651
Enzymes that do cut:
AarI AcuI AflIII AloI ApaI ApaLI ApoI AvaI
BamHI BanI BanII BbsI BbvCI BglI BglII BlpI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaBI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BssSI BstXI BstYI Bsu36I BtsI CspCI DraI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I Hin4I MmeI
MslI NspI PasI PciI PflMI PfoI PspOMI PsrI
PstI SacI ScaI SfcI SfiI SmlI SspI StuI
StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AlwNI AscI AseI AsiSI AvrII BaeI
BbeI BcgI BciVI BclI BfrBI BmgBI BmtI BplI
BsaAI BsaHI BsaWI BsiEI BsiWI BsmBI BspEI BsrBI
BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstZ17I
BtgI BtgZI ClaI DraIII DrdI EagI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MscI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PvuI
PvuII RsrII SacII SalI SanDI SapI SbfI SexAI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SwaI Tth111I XbaI XcmI XhoI XmaI ZraI