Restriction map for
pF1KB4108
(Linear) (Six-base) MAPSORT of: KIBB4108.seq Check: 7446 from: 1 to: 2931
KIBB4108.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AccI GT'mk_AC
Cuts at: 0 2726 2931
Size: 2726 205
Acc65I G'GTAC_C
Cuts at: 0 2000 2931
Size: 2000 931
AclI AA'CG_TT
Cuts at: 0 1687 2931
Size: 1687 1244
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 664 1261 1443 2201 2931
Size: 664 597 182 758 730
Fragments arranged by size:
758 730 664 597 182
AflII C'TTAA_G
Cuts at: 0 267 2931
Size: 267 2664
AflIII A'CryG_T
Cuts at: 0 994 1406 1841 2931
Size: 994 412 435 1090
Fragments arranged by size:
1090 994 435 412
AleI CACnn'nnGTG
Cuts at: 0 738 1423 2028 2931
Size: 738 685 605 903
Fragments arranged by size:
903 738 685 605
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1691 2931
Size: 1691 1240
AlwNI CAG_nnn'CTG
Cuts at: 0 687 927 1710 2931
Size: 687 240 783 1221
Fragments arranged by size:
1221 783 687 240
ApoI r'AATT_y
Cuts at: 0 167 412 706 1494 1771 1906 2316 2376
Size: 167 245 294 788 277 135 410 60
Cuts at: 2376 2508 2931
Size: 132 423
Fragments arranged by size:
788 423 410 294 277 245 167 135
132 60
AseI AT'TA_AT
Cuts at: 0 188 2931
Size: 188 2743
AvaI C'yCGr_G
Cuts at: 0 1980 2663 2931
Size: 1980 683 268
Fragments arranged by size:
1980 683 268
BanI G'GyrC_C
Cuts at: 0 378 1789 2000 2931
Size: 378 1411 211 931
Fragments arranged by size:
1411 931 378 211
BanII G_rGCy'C
Cuts at: 0 254 1733 1987 2931
Size: 254 1479 254 944
Fragments arranged by size:
1479 944 254 254
BbsI GAAGACnn'nnnn_
Cuts at: 0 975 1017 2109 2303 2931
Size: 975 42 1092 194 628
Fragments arranged by size:
1092 975 628 194 42
BclI T'GATC_A
Cuts at: 0 2006 2931
Size: 2006 925
BfrBI ATG'CAT
Cuts at: 0 1678 2931
Size: 1678 1253
BlpI GC'TnA_GC
Cuts at: 0 2570 2931
Size: 2570 361
Bme1580I G_kGCm'C
Cuts at: 0 1383 2931
Size: 1383 1548
BmrI ACTGGGnnnn_n'
Cuts at: 0 984 2764 2931
Size: 984 1780 167
Fragments arranged by size:
1780 984 167
BmtI G_CTAG'C
Cuts at: 0 1585 2931
Size: 1585 1346
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 404 567 981 2931
Size: 404 163 414 1950
Fragments arranged by size:
1950 414 404 163
Bpu10I CC'TnA_GC
Cuts at: 0 755 2689 2931
Size: 755 1934 242
Fragments arranged by size:
1934 755 242
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2118 2659 2931
Size: 2118 541 272
Fragments arranged by size:
2118 541 272
BsaI GGTCTCn'nnnn_
Cuts at: 0 38 2707 2931
Size: 38 2669 224
Fragments arranged by size:
2669 224 38
BsaAI yAC'GTr
Cuts at: 0 2916 2931
Size: 2916 15
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 485 515 565 595 1096 1126 2931
Size: 485 30 50 30 501 30 1805
Fragments arranged by size:
1805 501 485 50 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 211 978 2931
Size: 211 767 1953
Fragments arranged by size:
1953 767 211
BseYI C'CCAG_C
Cuts at: 0 905 2931
Size: 905 2026
BsiHKAI G_wGCw'C
Cuts at: 0 254 2931
Size: 254 2677
BsmI GAATG_Cn'
Cuts at: 0 117 544 1538 2301 2693 2931
Size: 117 427 994 763 392 238
Fragments arranged by size:
994 763 427 392 238 117
Bsp1286I G_dGCh'C
Cuts at: 0 254 1383 1733 1987 2931
Size: 254 1129 350 254 944
Fragments arranged by size:
1129 944 350 254 254
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1651 2931
Size: 1651 1280
BsrDI GCAATG_nn'
Cuts at: 0 224 623 2165 2460 2931
Size: 224 399 1542 295 471
Fragments arranged by size:
1542 471 399 295 224
BssSI C'ACGA_G
Cuts at: 0 514 1391 2931
Size: 514 877 1540
Fragments arranged by size:
1540 877 514
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2428 2931
Size: 2428 503
BstEII G'GTnAC_C
Cuts at: 0 2683 2931
Size: 2683 248
BstXI CCAn_nnnn'nTGG
Cuts at: 0 792 1425 2931
Size: 792 633 1506
Fragments arranged by size:
1506 792 633
BstYI r'GATC_y
Cuts at: 0 642 2015 2931
Size: 642 1373 916
Fragments arranged by size:
1373 916 642
BtgI C'CryG_G
Cuts at: 0 1222 2931
Size: 1222 1709
BtsI GCAGTG_nn'
Cuts at: 0 745 986 1699 2931
Size: 745 241 713 1232
Fragments arranged by size:
1232 745 713 241
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1782 1817 2931
Size: 1782 35 1114
Fragments arranged by size:
1782 1114 35
DraI TTT'AAA
Cuts at: 0 177 2931
Size: 177 2754
DraIII CAC_nnn'GTG
Cuts at: 0 2683 2931
Size: 2683 248
DrdI GACnn_nn'nnGTC
Cuts at: 0 1704 2931
Size: 1704 1227
EaeI y'GGCC_r
Cuts at: 0 608 872 2931
Size: 608 264 2059
Fragments arranged by size:
2059 608 264
EarI CTCTTCn'nnn_
Cuts at: 0 719 2192 2849 2931
Size: 719 1473 657 82
Fragments arranged by size:
1473 719 657 82
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1286 2931
Size: 1286 1645
EcoICRI GAG'CTC
Cuts at: 0 252 2931
Size: 252 2679
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 404 567 664 981 1261 1443 2201 2931
Size: 404 163 97 317 280 182 758 730
Fragments arranged by size:
758 730 404 317 280 182 163 97
EcoO109I rG'GnC_Cy
Cuts at: 0 759 1227 1507 2931
Size: 759 468 280 1424
Fragments arranged by size:
1424 759 468 280
EcoRI G'AATT_C
Cuts at: 0 412 706 2931
Size: 412 294 2225
Fragments arranged by size:
2225 412 294
EcoRV GAT'ATC
Cuts at: 0 2523 2931
Size: 2523 408
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 35 67 578 610 1058 1090 2091 2123
Size: 35 32 511 32 448 32 1001 32
Cuts at: 2123 2766 2798 2931
Size: 643 32 133
Fragments arranged by size:
1001 643 511 448 133 35 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 919 1640 2620 2931
Size: 919 721 980 311
Fragments arranged by size:
980 919 721 311
HindIII A'AGCT_T
Cuts at: 0 270 1921 2931
Size: 270 1651 1010
Fragments arranged by size:
1651 1010 270
HpaI GTT'AAC
Cuts at: 0 1640 2931
Size: 1640 1291
KpnI G_GTAC'C
Cuts at: 0 2004 2931
Size: 2004 927
MfeI C'AATT_G
Cuts at: 0 230 912 1606 2426 2931
Size: 230 682 694 820 505
Fragments arranged by size:
820 694 682 505 230
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 267 306 695 2931
Size: 267 39 389 2236
Fragments arranged by size:
2236 389 267 39
MscI TGG'CCA
Cuts at: 0 610 874 2931
Size: 610 264 2057
Fragments arranged by size:
2057 610 264
MslI CAynn'nnrTG
Cuts at: 0 738 1423 2028 2931
Size: 738 685 605 903
Fragments arranged by size:
903 738 685 605
MspA1I CmG'CkG
Cuts at: 0 524 924 2931
Size: 524 400 2007
Fragments arranged by size:
2007 524 400
NdeI CA'TA_TG
Cuts at: 0 1240 2931
Size: 1240 1691
NheI G'CTAG_C
Cuts at: 0 1581 2931
Size: 1581 1350
NsiI A_TGCA'T
Cuts at: 0 1680 2931
Size: 1680 1251
NspI r_CATG'y
Cuts at: 0 307 998 1410 1845 2848 2931
Size: 307 691 412 435 1003 83
Fragments arranged by size:
1003 691 435 412 307 83
PasI CC'CwG_GG
Cuts at: 0 2486 2931
Size: 2486 445
PciI A'CATG_T
Cuts at: 0 994 1406 1841 2931
Size: 994 412 435 1090
Fragments arranged by size:
1090 994 435 412
PfoI T'CCnGG_A
Cuts at: 0 1538 2931
Size: 1538 1393
PpuMI rG'GwC_Cy
Cuts at: 0 759 1227 1507 2931
Size: 759 468 280 1424
Fragments arranged by size:
1424 759 468 280
PshAI GACnn'nnGTC
Cuts at: 0 824 1753 2931
Size: 824 929 1178
Fragments arranged by size:
1178 929 824
PstI C_TGCA'G
Cuts at: 0 1706 2631 2931
Size: 1706 925 300
Fragments arranged by size:
1706 925 300
PvuII CAG'CTG
Cuts at: 0 924 2931
Size: 924 2007
SacI G_AGCT'C
Cuts at: 0 254 2931
Size: 254 2677
SapI GCTCTTCn'nnn_
Cuts at: 0 719 2931
Size: 719 2212
SexAI A'CCwGG_T
Cuts at: 0 2135 2931
Size: 2135 796
SfcI C'TryA_G
Cuts at: 0 39 1702 2627 2931
Size: 39 1663 925 304
Fragments arranged by size:
1663 925 304 39
SmlI C'TyrA_G
Cuts at: 0 267 2097 2638 2663 2931
Size: 267 1830 541 25 268
Fragments arranged by size:
1830 541 268 267 25
SspI AAT'ATT
Cuts at: 0 186 1724 2649 2931
Size: 186 1538 925 282
Fragments arranged by size:
1538 925 282 186
StuI AGG'CCT
Cuts at: 0 604 2189 2931
Size: 604 1585 742
Fragments arranged by size:
1585 742 604
StyI C'CwwG_G
Cuts at: 0 605 2931
Size: 605 2326
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2209 2533 2917 2931
Size: 2209 324 384 14
Fragments arranged by size:
2209 384 324 14
TatI w'GTAC_w
Cuts at: 0 1373 1610 2931
Size: 1373 237 1321
Fragments arranged by size:
1373 1321 237
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 485 517 565 597 1096 1128 2762 2794
Size: 485 32 48 32 499 32 1634 32
Cuts at: 2794 2931
Size: 137
Fragments arranged by size:
1634 499 485 137 48 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 2896 2931
Size: 2896 35
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 555 1397 2931
Size: 555 842 1534
Fragments arranged by size:
1534 842 555
XhoI C'TCGA_G
Cuts at: 0 2663 2931
Size: 2663 268
XmnI GAAnn'nnTTC
Cuts at: 0 800 2931
Size: 800 2131
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AleI AlfI
AlwNI ApoI AseI AvaI BanI BanII BbsI BclI
BfrBI BlpI Bme1580I BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I
BspMI BsrDI BssSI BstAPI BstEII BstXI BstYI BtgI
BtsI CspCI DraI DraIII DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV Hin4I HincII HindIII
HpaI KpnI MfeI MmeI MscI MslI MspA1I NdeI
NheI NsiI NspI PasI PciI PfoI PpuMI PshAI
PstI PvuII SacI SapI SexAI SfcI SmlI SspI
StuI StyI TaqII TatI TstI Tth111I XcmI XhoI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AloI ApaI ApaLI
AscI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI
BciVI BglI BglII BmgBI BplI BsaBI BsaHI BsaWI
BsgI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BstBI BstZ17I Bsu36I BtgZI ClaI EagI
EcoNI FalI FseI FspI FspAI HaeII KasI MluI
NaeI NarI NcoI NgoMIV NotI NruI PacI PflMI
PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI
XbaI XmaI ZraI