Restriction map for
pF1KB4113
(Linear) (Six-base) MAPSORT of: KIBB4113.seq Check: 4151 from: 1 to: 1572
KIBB4113.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 0 1316 1529 1572
Size: 1316 213 43
Fragments arranged by size:
1316 213 43
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 422 1052 1451 1572
Size: 64 358 630 399 121
Fragments arranged by size:
630 399 358 121 64
AfeI AGC'GCT
Cuts at: 0 37 1572
Size: 37 1535
AgeI A'CCGG_T
Cuts at: 0 1331 1572
Size: 1331 241
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 95 127 1572
Size: 95 32 1445
Fragments arranged by size:
1445 95 32
ApoI r'AATT_y
Cuts at: 0 145 170 701 1572
Size: 145 25 531 871
Fragments arranged by size:
871 531 145 25
AseI AT'TA_AT
Cuts at: 0 208 1572
Size: 208 1364
BbsI GAAGACnn'nnnn_
Cuts at: 0 827 1490 1572
Size: 827 663 82
Fragments arranged by size:
827 663 82
BciVI GTATCCnnnnn_n'
Cuts at: 0 267 1572
Size: 267 1305
BclI T'GATC_A
Cuts at: 0 1304 1572
Size: 1304 268
BglII A'GATC_T
Cuts at: 0 429 1572
Size: 429 1143
Bme1580I G_kGCm'C
Cuts at: 0 1235 1572
Size: 1235 337
BmrI ACTGGGnnnn_n'
Cuts at: 0 63 834 881 1572
Size: 63 771 47 691
Fragments arranged by size:
771 691 63 47
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1079 1572
Size: 1079 493
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 1429 1572
Size: 185 1244 143
Fragments arranged by size:
1244 185 143
BsaWI w'CCGG_w
Cuts at: 0 259 1331 1572
Size: 259 1072 241
Fragments arranged by size:
1072 259 241
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 95 125 1572
Size: 95 30 1447
Fragments arranged by size:
1447 95 30
BseYI C'CCAG_C
Cuts at: 0 514 799 843 1572
Size: 514 285 44 729
Fragments arranged by size:
729 514 285 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 1572
Size: 224 1348
BsiWI C'GTAC_G
Cuts at: 0 924 1572
Size: 924 648
Bsp1286I G_dGCh'C
Cuts at: 0 1235 1572
Size: 1235 337
BspEI T'CCGG_A
Cuts at: 0 259 1572
Size: 259 1313
BspHI T'CATG_A
Cuts at: 0 560 983 1572
Size: 560 423 589
Fragments arranged by size:
589 560 423
BsrDI GCAATG_nn'
Cuts at: 0 308 1307 1572
Size: 308 999 265
Fragments arranged by size:
999 308 265
BsrFI r'CCGG_y
Cuts at: 0 9 1331 1572
Size: 9 1322 241
Fragments arranged by size:
1322 241 9
BssHII G'CGCG_C
Cuts at: 0 276 1572
Size: 276 1296
BstEII G'GTnAC_C
Cuts at: 0 1248 1572
Size: 1248 324
BstXI CCAn_nnnn'nTGG
Cuts at: 0 957 1572
Size: 957 615
BstYI r'GATC_y
Cuts at: 0 429 1572
Size: 429 1143
BtgI C'CryG_G
Cuts at: 0 71 77 950 1253 1274 1343 1572
Size: 71 6 873 303 21 69 229
Fragments arranged by size:
873 303 229 71 69 21 6
BtsI GCAGTG_nn'
Cuts at: 0 1006 1130 1572
Size: 1006 124 442
Fragments arranged by size:
1006 442 124
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 291 326 1455 1490 1572
Size: 291 35 1129 35 82
Fragments arranged by size:
1129 291 82 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 543 1572
Size: 543 1029
EaeI y'GGCC_r
Cuts at: 0 74 857 1199 1572
Size: 74 783 342 373
Fragments arranged by size:
783 373 342 74
EarI CTCTTCn'nnn_
Cuts at: 0 536 996 1572
Size: 536 460 576
Fragments arranged by size:
576 536 460
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 185 422 1052 1429 1451 1572
Size: 64 121 237 630 377 22 121
Fragments arranged by size:
630 377 237 121 121 64 22
HaeII r_GCGC'y
Cuts at: 0 39 1572
Size: 39 1533
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1411 1443 1531 1563 1572
Size: 1411 32 88 32 9
Fragments arranged by size:
1411 88 32 32 9
HincII GTy'rAC
Cuts at: 0 1317 1350 1437 1572
Size: 1317 33 87 135
Fragments arranged by size:
1317 135 87 33
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 275 1572
Size: 275 1297
MscI TGG'CCA
Cuts at: 0 859 1572
Size: 859 713
MslI CAynn'nnrTG
Cuts at: 0 955 1572
Size: 955 617
MspA1I CmG'CkG
Cuts at: 0 79 556 906 1025 1132 1572
Size: 79 477 350 119 107 440
Fragments arranged by size:
477 440 350 119 107 79
NcoI C'CATG_G
Cuts at: 0 950 1253 1343 1572
Size: 950 303 90 229
Fragments arranged by size:
950 303 229 90
NspI r_CATG'y
Cuts at: 0 615 1572
Size: 615 957
PflMI CCAn_nnn'nTGG
Cuts at: 0 369 1572
Size: 369 1203
PfoI T'CCnGG_A
Cuts at: 0 162 1325 1572
Size: 162 1163 247
Fragments arranged by size:
1163 247 162
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 95 127 1572
Size: 95 32 1445
Fragments arranged by size:
1445 95 32
PshAI GACnn'nnGTC
Cuts at: 0 1345 1572
Size: 1345 227
PvuII CAG'CTG
Cuts at: 0 556 1572
Size: 556 1016
SacII CC_GC'GG
Cuts at: 0 80 1572
Size: 80 1492
SalI G'TCGA_C
Cuts at: 0 1315 1572
Size: 1315 257
SexAI A'CCwGG_T
Cuts at: 0 1043 1572
Size: 1043 529
SspI AAT'ATT
Cuts at: 0 833 1572
Size: 833 739
StuI AGG'CCT
Cuts at: 0 469 1572
Size: 469 1103
StyI C'CwwG_G
Cuts at: 0 950 1253 1343 1356 1457 1572
Size: 950 303 90 13 101 115
Fragments arranged by size:
950 303 115 101 90 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 45 361 376 1570 1572
Size: 45 316 15 1194 2
Fragments arranged by size:
1194 316 45 15 2
TatI w'GTAC_w
Cuts at: 0 482 1572
Size: 482 1090
XbaI T'CTAG_A
Cuts at: 0 138 1572
Size: 138 1434
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 105 1404 1572
Size: 105 1299 168
Fragments arranged by size:
1299 168 105
XmnI GAAnn'nnTTC
Cuts at: 0 37 445 937 1572
Size: 37 408 492 635
Fragments arranged by size:
635 492 408 37
Enzymes that do cut:
AccI AcuI AfeI AgeI AloI ApoI AseI BbsI
BciVI BclI BglII Bme1580I BmrI BplI BpmI BsaWI
BsaXI BseYI BsgI BsiWI Bsp1286I BspEI BspHI BsrDI
BsrFI BssHII BstEII BstXI BstYI BtgI BtsI CspCI
DrdI EaeI EarI Eco57MI HaeII Hin4I HincII MmeI
MscI MslI MspA1I NcoI NspI PflMI PfoI PpiI
PshAI PvuII SacII SalI SexAI SspI StuI StyI
TaqII TatI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AhdI AleI
AlfI AlwNI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BamHI BanI BanII BbeI BbvCI BcgI BfrBI
BglI BlpI BmgBI BmtI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BseRI BsiEI BsiHKAI BsmI BsmBI BspMI
BsrBI BsrGI BssSI BstAPI BstBI BstZ17I Bsu36I BtgZI
ClaI DraI DraIII EagI EciI EcoICRI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PmeI
PmlI PpuMI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SanDI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SwaI TstI Tth111I XhoI XmaI ZraI