Restriction map for
pF1KB4115
(Linear) (Six-base) MAPSORT of: KIBB4115.seq Check: 3262 from: 1 to: 2814
KIBB4115.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AccI GT'mk_AC
Cuts at: 0 1155 2814
Size: 1155 1659
AclI AA'CG_TT
Cuts at: 0 254 2814
Size: 254 2560
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 1102 1475 1553 2357 2814
Size: 383 719 373 78 804 457
Fragments arranged by size:
804 719 457 383 373 78
AflIII A'CryG_T
Cuts at: 0 1866 2814
Size: 1866 948
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1043 2814
Size: 1043 1771
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 74 106 2814
Size: 74 32 2708
Fragments arranged by size:
2708 74 32
ApoI r'AATT_y
Cuts at: 0 1480 2211 2814
Size: 1480 731 603
Fragments arranged by size:
1480 731 603
AvrII C'CTAG_G
Cuts at: 0 1971 2814
Size: 1971 843
BanI G'GyrC_C
Cuts at: 0 2320 2814
Size: 2320 494
BanII G_rGCy'C
Cuts at: 0 2002 2352 2693 2814
Size: 2002 350 341 121
Fragments arranged by size:
2002 350 341 121
BbsI GAAGACnn'nnnn_
Cuts at: 0 539 2814
Size: 539 2275
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 784 818 2814
Size: 784 34 1996
Fragments arranged by size:
1996 784 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2521 2814
Size: 2521 293
BclI T'GATC_A
Cuts at: 0 609 2814
Size: 609 2205
BglI GCCn_nnn'nGGC
Cuts at: 0 118 2814
Size: 118 2696
BglII A'GATC_T
Cuts at: 0 699 1887 2814
Size: 699 1188 927
Fragments arranged by size:
1188 927 699
Bme1580I G_kGCm'C
Cuts at: 0 2325 2814
Size: 2325 489
BmgBI CAC'GTC
Cuts at: 0 2497 2814
Size: 2497 317
BmrI ACTGGGnnnn_n'
Cuts at: 0 1642 2408 2814
Size: 1642 766 406
Fragments arranged by size:
1642 766 406
BmtI G_CTAG'C
Cuts at: 0 1543 2814
Size: 1543 1271
BsaI GGTCTCn'nnnn_
Cuts at: 0 732 2814
Size: 732 2082
BsaBI GATnn'nnATC
Cuts at: 0 338 1595 2567 2814
Size: 338 1257 972 247
Fragments arranged by size:
1257 972 338 247
BsaHI Gr'CG_yC
Cuts at: 0 1470 2814
Size: 1470 1344
BsaWI w'CCGG_w
Cuts at: 0 1841 2814
Size: 1841 973
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 74 104 2814
Size: 74 30 2710
Fragments arranged by size:
2710 74 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1100 1495 1979 2814
Size: 1100 395 484 835
Fragments arranged by size:
1100 835 484 395
BseYI C'CCAG_C
Cuts at: 0 67 2176 2814
Size: 67 2109 638
Fragments arranged by size:
2109 638 67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 722 1399 2219 2814
Size: 722 677 820 595
Fragments arranged by size:
820 722 677 595
BsmI GAATG_Cn'
Cuts at: 0 352 1908 2814
Size: 352 1556 906
Fragments arranged by size:
1556 906 352
Bsp1286I G_dGCh'C
Cuts at: 0 2002 2325 2352 2693 2814
Size: 2002 323 27 341 121
Fragments arranged by size:
2002 341 323 121 27
BspEI T'CCGG_A
Cuts at: 0 1841 2814
Size: 1841 973
BsrBI CCG'CTC
Cuts at: 0 1774 1986 2814
Size: 1774 212 828
Fragments arranged by size:
1774 828 212
BsrDI GCAATG_nn'
Cuts at: 0 1901 2099 2814
Size: 1901 198 715
Fragments arranged by size:
1901 715 198
BsrFI r'CCGG_y
Cuts at: 0 2606 2814
Size: 2606 208
BssSI C'ACGA_G
Cuts at: 0 1727 2814
Size: 1727 1087
BstEII G'GTnAC_C
Cuts at: 0 1831 2814
Size: 1831 983
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2524 2814
Size: 2524 290
BstYI r'GATC_y
Cuts at: 0 187 699 1022 1887 2814
Size: 187 512 323 865 927
Fragments arranged by size:
927 865 512 323 187
BtgI C'CryG_G
Cuts at: 0 1584 1677 2517 2814
Size: 1584 93 840 297
Fragments arranged by size:
1584 840 297 93
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 347 2814
Size: 347 2467
BtsI GCAGTG_nn'
Cuts at: 0 992 2814
Size: 992 1822
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1650 1685 2298 2333 2814
Size: 1650 35 613 35 481
Fragments arranged by size:
1650 613 481 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1648 2263 2337 2814
Size: 1648 615 74 477
Fragments arranged by size:
1648 615 477 74
EaeI y'GGCC_r
Cuts at: 0 1073 1343 1658 1758 2565 2814
Size: 1073 270 315 100 807 249
Fragments arranged by size:
1073 807 315 270 249 100
EarI CTCTTCn'nnn_
Cuts at: 0 135 388 449 521 602 659 1494 1529
Size: 135 253 61 72 81 57 835 35
Cuts at: 1529 1764 2814
Size: 235 1050
Fragments arranged by size:
1050 835 253 235 135 81 72 61
57 35
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 1102 1475 1553 2357 2814
Size: 383 719 373 78 804 457
Fragments arranged by size:
804 719 457 383 373 78
EcoNI CCTnn'n_nnAGG
Cuts at: 0 749 1120 1555 2063 2814
Size: 749 371 435 508 751
Fragments arranged by size:
751 749 508 435 371
EcoO109I rG'GnC_Cy
Cuts at: 0 1113 2814
Size: 1113 1701
EcoRI G'AATT_C
Cuts at: 0 2211 2814
Size: 2211 603
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1220 2145 2814
Size: 1220 925 669
Fragments arranged by size:
1220 925 669
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 612 644 981 1013 2460 2492 2814
Size: 612 32 337 32 1447 32 322
Fragments arranged by size:
1447 612 337 322 32 32 32
HincII GTy'rAC
Cuts at: 0 1156 2361 2814
Size: 1156 1205 453
Fragments arranged by size:
1205 1156 453
HindIII A'AGCT_T
Cuts at: 0 473 2663 2814
Size: 473 2190 151
Fragments arranged by size:
2190 473 151
MfeI C'AATT_G
Cuts at: 0 566 980 2814
Size: 566 414 1834
Fragments arranged by size:
1834 566 414
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 941 2245 2620 2814
Size: 941 1304 375 194
Fragments arranged by size:
1304 941 375 194
MscI TGG'CCA
Cuts at: 0 1345 1660 2567 2814
Size: 1345 315 907 247
Fragments arranged by size:
1345 907 315 247
MslI CAynn'nnrTG
Cuts at: 0 2504 2522 2561 2814
Size: 2504 18 39 253
Fragments arranged by size:
2504 253 39 18
MspA1I CmG'CkG
Cuts at: 0 2758 2814
Size: 2758 56
NaeI GCC'GGC
Cuts at: 0 2608 2814
Size: 2608 206
NcoI C'CATG_G
Cuts at: 0 1584 2517 2814
Size: 1584 933 297
Fragments arranged by size:
1584 933 297
NgoMIV G'CCGG_C
Cuts at: 0 2606 2814
Size: 2606 208
NheI G'CTAG_C
Cuts at: 0 1539 2814
Size: 1539 1275
PflMI CCAn_nnn'nTGG
Cuts at: 0 508 973 2296 2814
Size: 508 465 1323 518
Fragments arranged by size:
1323 518 508 465
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 74 106 2814
Size: 74 32 2708
Fragments arranged by size:
2708 74 32
PstI C_TGCA'G
Cuts at: 0 915 2814
Size: 915 1899
PvuII CAG'CTG
Cuts at: 0 2758 2814
Size: 2758 56
SalI G'TCGA_C
Cuts at: 0 1154 2814
Size: 1154 1660
SapI GCTCTTCn'nnn_
Cuts at: 0 135 1529 1764 2814
Size: 135 1394 235 1050
Fragments arranged by size:
1394 1050 235 135
SexAI A'CCwGG_T
Cuts at: 0 966 1835 2814
Size: 966 869 979
Fragments arranged by size:
979 966 869
SfcI C'TryA_G
Cuts at: 0 911 1977 2814
Size: 911 1066 837
Fragments arranged by size:
1066 911 837
SpeI A'CTAG_T
Cuts at: 0 2194 2814
Size: 2194 620
StuI AGG'CCT
Cuts at: 0 1970 2050 2814
Size: 1970 80 764
Fragments arranged by size:
1970 764 80
StyI C'CwwG_G
Cuts at: 0 344 1109 1584 1811 1971 2279 2352 2517
Size: 344 765 475 227 160 308 73 165
Cuts at: 2517 2814
Size: 297
Fragments arranged by size:
765 475 344 308 297 227 165 160
73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2177 2814
Size: 2177 637
TatI w'GTAC_w
Cuts at: 0 2117 2814
Size: 2117 697
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 32 2814
Size: 32 2782
Tth111I GACn'n_nGTC
Cuts at: 0 1837 2814
Size: 1837 977
XbaI T'CTAG_A
Cuts at: 0 540 2814
Size: 540 2274
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 112 2070 2814
Size: 112 1958 744
Fragments arranged by size:
1958 744 112
Enzymes that do cut:
AccI AclI AcuI AflIII AlfI AloI ApoI AvrII
BanI BanII BbsI BcgI BciVI BclI BglI BglII
Bme1580I BmgBI BmrI BmtI BsaI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsmI Bsp1286I BspEI BsrBI
BsrDI BsrFI BssSI BstEII BstXI BstYI BtgI BtgZI
BtsI CspCI DrdI EaeI EarI Eco57MI EcoNI EcoO109I
EcoRI FalI Hin4I HincII HindIII MfeI MmeI MscI
MslI MspA1I NaeI NcoI NgoMIV NheI PflMI PpiI
PstI PvuII SalI SapI SexAI SfcI SpeI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AgeI AhdI AleI
AlwNI ApaI ApaLI AscI AseI AsiSI AvaI BaeI
BamHI BbeI BbvCI BfrBI BlpI BplI BpmI Bpu10I
BpuEI BsaAI BsiEI BsiHKAI BsiWI BsmBI BspHI BspMI
BsrGI BssHII BstAPI BstBI BstZ17I Bsu36I ClaI DraI
DraIII EagI EciI EcoICRI EcoRV FseI FspI FspAI
HaeII HpaI KasI KpnI MluI NarI NdeI NotI
NruI NsiI NspI PacI PasI PciI PfoI PmeI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SanDI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SphI SrfI SspI
SwaI XhoI XmaI XmnI ZraI