Restriction map for
pF1KB4121
(Linear) (Six-base) MAPSORT of: KIBB4121.seq Check: 328 from: 1 to: 1809
KIBB4121.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AatII G_ACGT'C
Cuts at: 0 67 1809
Size: 67 1742
AccI GT'mk_AC
Cuts at: 0 1027 1809
Size: 1027 782
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1079 1809
Size: 1079 730
AflIII A'CryG_T
Cuts at: 0 419 1809
Size: 419 1390
ApoI r'AATT_y
Cuts at: 0 312 671 809 1249 1369 1522 1809
Size: 312 359 138 440 120 153 287
Fragments arranged by size:
440 359 312 287 153 138 120
AseI AT'TA_AT
Cuts at: 0 563 1809
Size: 563 1246
AvaI C'yCGr_G
Cuts at: 0 68 270 1454 1809
Size: 68 202 1184 355
Fragments arranged by size:
1184 355 202 68
BanII G_rGCy'C
Cuts at: 0 581 1679 1809
Size: 581 1098 130
Fragments arranged by size:
1098 581 130
BfrBI ATG'CAT
Cuts at: 0 898 1809
Size: 898 911
BglI GCCn_nnn'nGGC
Cuts at: 0 865 1809
Size: 865 944
BglII A'GATC_T
Cuts at: 0 87 1809
Size: 87 1722
BlpI GC'TnA_GC
Cuts at: 0 1148 1809
Size: 1148 661
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 83 1809
Size: 83 1726
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1300 1809
Size: 1300 509
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1732 1809
Size: 1732 77
BsaBI GATnn'nnATC
Cuts at: 0 899 1809
Size: 899 910
BsaHI Gr'CG_yC
Cuts at: 0 64 1809
Size: 64 1745
BsaWI w'CCGG_w
Cuts at: 0 638 1809
Size: 638 1171
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 34 64 1809
Size: 34 30 1745
Fragments arranged by size:
1745 34 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 320 890 1809
Size: 320 570 919
Fragments arranged by size:
919 570 320
BseYI C'CCAG_C
Cuts at: 0 841 1809
Size: 841 968
BsiHKAI G_wGCw'C
Cuts at: 0 581 1679 1809
Size: 581 1098 130
Fragments arranged by size:
1098 581 130
BsmBI CGTCTCn'nnnn_
Cuts at: 0 71 1809
Size: 71 1738
Bsp1286I G_dGCh'C
Cuts at: 0 581 1679 1809
Size: 581 1098 130
Fragments arranged by size:
1098 581 130
BspEI T'CCGG_A
Cuts at: 0 638 1809
Size: 638 1171
BsrDI GCAATG_nn'
Cuts at: 0 1119 1809
Size: 1119 690
BssSI C'ACGA_G
Cuts at: 0 411 1809
Size: 411 1398
BstAPI GCAn_nnn'nTGC
Cuts at: 0 531 1785 1809
Size: 531 1254 24
Fragments arranged by size:
1254 531 24
BstBI TT'CG_AA
Cuts at: 0 1208 1809
Size: 1208 601
BstYI r'GATC_y
Cuts at: 0 87 404 1809
Size: 87 317 1405
Fragments arranged by size:
1405 317 87
Bsu36I CC'TnA_GG
Cuts at: 0 355 1809
Size: 355 1454
BtsI GCAGTG_nn'
Cuts at: 0 860 1131 1809
Size: 860 271 678
Fragments arranged by size:
860 678 271
DraI TTT'AAA
Cuts at: 0 461 962 1809
Size: 461 501 847
Fragments arranged by size:
847 501 461
EaeI y'GGCC_r
Cuts at: 0 857 1163 1809
Size: 857 306 646
Fragments arranged by size:
857 646 306
EarI CTCTTCn'nnn_
Cuts at: 0 177 384 1096 1809
Size: 177 207 712 713
Fragments arranged by size:
713 712 207 177
EcoICRI GAG'CTC
Cuts at: 0 579 1677 1809
Size: 579 1098 132
Fragments arranged by size:
1098 579 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1079 1300 1809
Size: 1079 221 509
Fragments arranged by size:
1079 509 221
EcoO109I rG'GnC_Cy
Cuts at: 0 659 1809
Size: 659 1150
EcoRI G'AATT_C
Cuts at: 0 671 1369 1809
Size: 671 698 440
Fragments arranged by size:
698 671 440
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 34 62 66 94 176 208 1809
Size: 34 28 4 28 82 32 1601
Fragments arranged by size:
1601 82 34 32 28 28 4
HindIII A'AGCT_T
Cuts at: 0 1741 1809
Size: 1741 68
MluI A'CGCG_T
Cuts at: 0 419 1809
Size: 419 1390
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1157 1809
Size: 1157 652
MscI TGG'CCA
Cuts at: 0 859 1165 1809
Size: 859 306 644
Fragments arranged by size:
859 644 306
MslI CAynn'nnrTG
Cuts at: 0 1124 1171 1289 1809
Size: 1124 47 118 520
Fragments arranged by size:
1124 520 118 47
MspA1I CmG'CkG
Cuts at: 0 1152 1809
Size: 1152 657
NdeI CA'TA_TG
Cuts at: 0 154 1809
Size: 154 1655
NsiI A_TGCA'T
Cuts at: 0 900 1809
Size: 900 909
PfoI T'CCnGG_A
Cuts at: 0 114 1809
Size: 114 1695
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1709 1741 1809
Size: 1709 32 68
Fragments arranged by size:
1709 68 32
PpuMI rG'GwC_Cy
Cuts at: 0 659 1809
Size: 659 1150
PsiI TTA'TAA
Cuts at: 0 1393 1809
Size: 1393 416
PstI C_TGCA'G
Cuts at: 0 1724 1809
Size: 1724 85
PvuII CAG'CTG
Cuts at: 0 1152 1809
Size: 1152 657
SacI G_AGCT'C
Cuts at: 0 581 1679 1809
Size: 581 1098 130
Fragments arranged by size:
1098 581 130
SfcI C'TryA_G
Cuts at: 0 1188 1358 1720 1809
Size: 1188 170 362 89
Fragments arranged by size:
1188 362 170 89
SmlI C'TyrA_G
Cuts at: 0 68 270 1454 1711 1809
Size: 68 202 1184 257 98
Fragments arranged by size:
1184 257 202 98 68
SspI AAT'ATT
Cuts at: 0 939 1809
Size: 939 870
StyI C'CwwG_G
Cuts at: 0 38 1160 1809
Size: 38 1122 649
Fragments arranged by size:
1122 649 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 250 1590 1809
Size: 250 1340 219
Fragments arranged by size:
1340 250 219
XbaI T'CTAG_A
Cuts at: 0 324 1809
Size: 324 1485
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 683 1173 1809
Size: 683 490 636
Fragments arranged by size:
683 636 490
XhoI C'TCGA_G
Cuts at: 0 68 270 1454 1809
Size: 68 202 1184 355
Fragments arranged by size:
1184 355 202 68
ZraI GAC'GTC
Cuts at: 0 65 1809
Size: 65 1744
Enzymes that do cut:
AatII AccI AcuI AflIII ApoI AseI AvaI BanII
BfrBI BglI BglII BlpI BplI BpmI BpuEI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI BsmBI Bsp1286I
BspEI BsrDI BssSI BstAPI BstBI BstYI Bsu36I BtsI
DraI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI Hin4I
HindIII MluI MmeI MscI MslI MspA1I NdeI NsiI
PfoI PpiI PpuMI PsiI PstI PvuII SacI SfcI
SmlI SspI StyI TaqII XbaI XcmI XhoI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AsiSI AvrII
BaeI BamHI BanI BbeI BbsI BbvCI BcgI BciVI
BclI Bme1580I BmgBI BmrI BmtI Bpu10I BsaI BsaAI
BsgI BsiEI BsiWI BsmI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BstEII BstXI BstZ17I BtgI BtgZI ClaI
CspCI DraIII DrdI EagI EciI EcoNI EcoRV FalI
FseI FspI FspAI HaeII HincII HpaI KasI KpnI
MfeI NaeI NarI NcoI NgoMIV NheI NotI NruI
NspI PacI PasI PciI PflMI PmeI PmlI PshAI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI SwaI TatI
TstI Tth111I XmaI XmnI