Restriction map for
pF1KB4125
(Linear) (Six-base) MAPSORT of: KIBB4125.seq Check: 8257 from: 1 to: 3150
KIBB4125.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 900 3150
Size: 900 2250
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 712 2702 2906 3150
Size: 712 1990 204 244
Fragments arranged by size:
1990 712 244 204
AfeI AGC'GCT
Cuts at: 0 238 259 3150
Size: 238 21 2891
Fragments arranged by size:
2891 238 21
AflIII A'CryG_T
Cuts at: 0 556 709 724 3150
Size: 556 153 15 2426
Fragments arranged by size:
2426 556 153 15
AgeI A'CCGG_T
Cuts at: 0 1151 3150
Size: 1151 1999
AlwNI CAG_nnn'CTG
Cuts at: 0 1468 2017 2116 3150
Size: 1468 549 99 1034
Fragments arranged by size:
1468 1034 549 99
ApaI G_GGCC'C
Cuts at: 0 68 115 1502 3150
Size: 68 47 1387 1648
Fragments arranged by size:
1648 1387 68 47
ApoI r'AATT_y
Cuts at: 0 853 1329 1983 2574 2932 3040 3067 3150
Size: 853 476 654 591 358 108 27 83
Fragments arranged by size:
853 654 591 476 358 108 83 27
BamHI G'GATC_C
Cuts at: 0 2415 3150
Size: 2415 735
BanI G'GyrC_C
Cuts at: 0 75 198 1160 3150
Size: 75 123 962 1990
Fragments arranged by size:
1990 962 123 75
BanII G_rGCy'C
Cuts at: 0 68 115 292 570 1242 1502 1837 2311
Size: 68 47 177 278 672 260 335 474
Cuts at: 2311 3150
Size: 839
Fragments arranged by size:
839 672 474 335 278 260 177 68
47
BbeI G_GCGC'C
Cuts at: 0 79 202 3150
Size: 79 123 2948
Fragments arranged by size:
2948 123 79
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 26 60 1150 1184 3150
Size: 26 34 1090 34 1966
Fragments arranged by size:
1966 1090 34 34 26
BclI T'GATC_A
Cuts at: 0 803 3150
Size: 803 2347
BfrBI ATG'CAT
Cuts at: 0 1080 1186 3150
Size: 1080 106 1964
Fragments arranged by size:
1964 1080 106
Bme1580I G_kGCm'C
Cuts at: 0 68 115 1502 2533 2564 3150
Size: 68 47 1387 1031 31 586
Fragments arranged by size:
1387 1031 586 68 47 31
BmgBI CAC'GTC
Cuts at: 0 557 3150
Size: 557 2593
BmrI ACTGGGnnnn_n'
Cuts at: 0 376 1186 2131 2648 3150
Size: 376 810 945 517 502
Fragments arranged by size:
945 810 517 502 376
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2527 3150
Size: 2527 623
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 611 1393 1985 2049 2170 3150
Size: 611 782 592 64 121 980
Fragments arranged by size:
980 782 611 592 121 64
Bpu10I CC'TnA_GC
Cuts at: 0 1304 3150
Size: 1304 1846
BsaI GGTCTCn'nnnn_
Cuts at: 0 292 3150
Size: 292 2858
BsaHI Gr'CG_yC
Cuts at: 0 76 199 3150
Size: 76 123 2951
Fragments arranged by size:
2951 123 76
BsaWI w'CCGG_w
Cuts at: 0 1151 1872 2187 3150
Size: 1151 721 315 963
Fragments arranged by size:
1151 963 721 315
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1148 1178 3150
Size: 1148 30 1972
Fragments arranged by size:
1972 1148 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1586 2335 2503 3150
Size: 1586 749 168 647
Fragments arranged by size:
1586 749 647 168
BseYI C'CCAG_C
Cuts at: 0 254 285 454 3150
Size: 254 31 169 2696
Fragments arranged by size:
2696 254 169 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 422 1805 2507 3150
Size: 205 217 1383 702 643
Fragments arranged by size:
1383 702 643 217 205
BsiEI CG_ry'CG
Cuts at: 0 296 3150
Size: 296 2854
BsiHKAI G_wGCw'C
Cuts at: 0 570 1837 2311 3150
Size: 570 1267 474 839
Fragments arranged by size:
1267 839 570 474
BsmI GAATG_Cn'
Cuts at: 0 1496 3150
Size: 1496 1654
Bsp1286I G_dGCh'C
Cuts at: 0 68 115 292 570 1242 1502 1837 2311
Size: 68 47 177 278 672 260 335 474
Cuts at: 2311 2533 2564 3150
Size: 222 31 586
Fragments arranged by size:
672 586 474 335 278 260 222 177
68 47 31
BspEI T'CCGG_A
Cuts at: 0 1872 3150
Size: 1872 1278
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 900 3150
Size: 900 2250
BsrBI CCG'CTC
Cuts at: 0 83 3150
Size: 83 3067
BsrFI r'CCGG_y
Cuts at: 0 330 1151 3150
Size: 330 821 1999
Fragments arranged by size:
1999 821 330
BsrGI T'GTAC_A
Cuts at: 0 487 2034 3096 3150
Size: 487 1547 1062 54
Fragments arranged by size:
1547 1062 487 54
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1077 1449 2606 3150
Size: 1077 372 1157 544
Fragments arranged by size:
1157 1077 544 372
BstBI TT'CG_AA
Cuts at: 0 2160 3150
Size: 2160 990
BstXI CCAn_nnnn'nTGG
Cuts at: 0 831 3150
Size: 831 2319
BstYI r'GATC_y
Cuts at: 0 869 2415 3150
Size: 869 1546 735
Fragments arranged by size:
1546 869 735
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 997 3150
Size: 997 2153
BtsI GCAGTG_nn'
Cuts at: 0 1722 3150
Size: 1722 1428
ClaI AT'CG_AT
Cuts at: 0 1826 3150
Size: 1826 1324
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 345 380 3150
Size: 345 35 2770
Fragments arranged by size:
2770 345 35
DraIII CAC_nnn'GTG
Cuts at: 0 1345 2059 3150
Size: 1345 714 1091
Fragments arranged by size:
1345 1091 714
DrdI GACnn_nn'nnGTC
Cuts at: 0 601 3150
Size: 601 2549
EaeI y'GGCC_r
Cuts at: 0 293 1941 3150
Size: 293 1648 1209
Fragments arranged by size:
1648 1209 293
EagI C'GGCC_G
Cuts at: 0 293 3150
Size: 293 2857
EarI CTCTTCn'nnn_
Cuts at: 0 1874 2100 2441 3150
Size: 1874 226 341 709
Fragments arranged by size:
1874 709 341 226
EcoICRI GAG'CTC
Cuts at: 0 568 1835 2309 3150
Size: 568 1267 474 841
Fragments arranged by size:
1267 841 568 474
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 611 712 1393 1985 2049 2170 2702 2906
Size: 611 101 681 592 64 121 532 204
Cuts at: 2906 3150
Size: 244
Fragments arranged by size:
681 611 592 532 244 204 121 101
64
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1003 3150
Size: 1003 2147
EcoO109I rG'GnC_Cy
Cuts at: 0 64 65 111 1498 2014 3150
Size: 64 1 46 1387 516 1136
Fragments arranged by size:
1387 1136 516 64 46 1
EcoRI G'AATT_C
Cuts at: 0 1329 2932 3040 3150
Size: 1329 1603 108 110
Fragments arranged by size:
1603 1329 110 108
EcoRV GAT'ATC
Cuts at: 0 1618 3150
Size: 1618 1532
HaeII r_GCGC'y
Cuts at: 0 79 202 240 261 2730 3150
Size: 79 123 38 21 2469 420
Fragments arranged by size:
2469 420 123 79 38 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 607 639 1819 1851 2224 2256 2374 2406
Size: 607 32 1180 32 373 32 118 32
Cuts at: 2406 3150
Size: 744
Fragments arranged by size:
1180 744 607 373 118 32 32 32
32
HincII GTy'rAC
Cuts at: 0 2699 2837 3150
Size: 2699 138 313
Fragments arranged by size:
2699 313 138
HindIII A'AGCT_T
Cuts at: 0 782 3150
Size: 782 2368
HpaI GTT'AAC
Cuts at: 0 2699 3150
Size: 2699 451
KasI G'GCGC_C
Cuts at: 0 75 198 3150
Size: 75 123 2952
Fragments arranged by size:
2952 123 75
MfeI C'AATT_G
Cuts at: 0 1063 3150
Size: 1063 2087
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 345 2052 2393 2424 3150
Size: 137 208 1707 341 31 726
Fragments arranged by size:
1707 726 341 208 137 31
MscI TGG'CCA
Cuts at: 0 1943 3150
Size: 1943 1207
MspA1I CmG'CkG
Cuts at: 0 40 338 690 3150
Size: 40 298 352 2460
Fragments arranged by size:
2460 352 298 40
NarI GG'CG_CC
Cuts at: 0 76 199 3150
Size: 76 123 2951
Fragments arranged by size:
2951 123 76
NdeI CA'TA_TG
Cuts at: 0 1415 3150
Size: 1415 1735
NsiI A_TGCA'T
Cuts at: 0 1082 1188 3150
Size: 1082 106 1962
Fragments arranged by size:
1962 1082 106
NspI r_CATG'y
Cuts at: 0 713 728 1075 3150
Size: 713 15 347 2075
Fragments arranged by size:
2075 713 347 15
PciI A'CATG_T
Cuts at: 0 709 724 3150
Size: 709 15 2426
Fragments arranged by size:
2426 709 15
PflMI CCAn_nnn'nTGG
Cuts at: 0 1345 3150
Size: 1345 1805
PpuMI rG'GwC_Cy
Cuts at: 0 2014 3150
Size: 2014 1136
PsiI TTA'TAA
Cuts at: 0 1856 3150
Size: 1856 1294
PspOMI G'GGCC_C
Cuts at: 0 64 111 1498 3150
Size: 64 47 1387 1652
Fragments arranged by size:
1652 1387 64 47
PvuII CAG'CTG
Cuts at: 0 690 3150
Size: 690 2460
SacI G_AGCT'C
Cuts at: 0 570 1837 2311 3150
Size: 570 1267 474 839
Fragments arranged by size:
1267 839 570 474
SapI GCTCTTCn'nnn_
Cuts at: 0 2100 3150
Size: 2100 1050
ScaI AGT'ACT
Cuts at: 0 1893 3150
Size: 1893 1257
SfcI C'TryA_G
Cuts at: 0 1666 2705 3150
Size: 1666 1039 445
Fragments arranged by size:
1666 1039 445
SfoI GGC'GCC
Cuts at: 0 77 200 3150
Size: 77 123 2950
Fragments arranged by size:
2950 123 77
SpeI A'CTAG_T
Cuts at: 0 1780 2029 3150
Size: 1780 249 1121
Fragments arranged by size:
1780 1121 249
SspI AAT'ATT
Cuts at: 0 1911 3150
Size: 1911 1239
StuI AGG'CCT
Cuts at: 0 2006 2247 2687 3150
Size: 2006 241 440 463
Fragments arranged by size:
2006 463 440 241
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 373 3150
Size: 373 2777
TatI w'GTAC_w
Cuts at: 0 460 487 698 1114 1891 2034 2353 2873
Size: 460 27 211 416 777 143 319 520
Cuts at: 2873 3096 3150
Size: 223 54
Fragments arranged by size:
777 520 460 416 319 223 211 143
54 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2117 2149 3150
Size: 2117 32 1001
Fragments arranged by size:
2117 1001 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2588 3150
Size: 2588 562
XmnI GAAnn'nnTTC
Cuts at: 0 785 2996 3150
Size: 785 2211 154
Fragments arranged by size:
2211 785 154
Enzymes that do cut:
AarI AcuI AfeI AflIII AgeI AlwNI ApaI ApoI
BamHI BanI BanII BbeI BcgI BclI BfrBI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BsaI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspMI BsrBI BsrFI BsrGI BstAPI BstBI BstXI
BstYI BtgZI BtsI ClaI CspCI DraIII DrdI EaeI
EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
HaeII Hin4I HincII HindIII HpaI KasI MfeI MmeI
MscI MspA1I NarI NdeI NsiI NspI PciI PflMI
PpuMI PsiI PspOMI PvuII SacI SapI ScaI SfcI
SfoI SpeI SspI StuI TaqII TatI TstI XcmI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AhdI AleI AlfI
AloI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BbsI BbvCI BciVI BglI BglII BlpI BmtI BpuEI
BsaAI BsaBI BsiWI BsmBI BspHI BsrDI BssHII BssSI
BstEII BstZ17I Bsu36I BtgI DraI EciI FalI FseI
FspI FspAI KpnI MluI MslI NaeI NcoI NgoMIV
NheI NotI NruI PacI PasI PfoI PmeI PmlI
PpiI PshAI PspXI PsrI PstI PvuI RsrII SacII
SalI SanDI SbfI SexAI SfiI SgfI SgrAI SmaI
SmlI SnaBI SphI SrfI StyI SwaI Tth111I XbaI
XhoI XmaI ZraI