Restriction map for pF1KB4126
 (Linear) (Six-base) MAPSORT of: KIBB4126.seq  Check: 9659  from: 1  to: 1299



KIBB4126.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:25  ..

AclI AA'CG_TT

Cuts at:      0     395    1299 
   Size:        395     904
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     688     842     918    1299 
   Size:        688     154      76     381

  Fragments arranged by size: 

                 688     381     154      76
 
AflIII A'CryG_T

Cuts at:      0     809    1299 
   Size:        809     490
 
AlwNI CAG_nnn'CTG

Cuts at:      0     865    1299 
   Size:        865     434
 
ApoI r'AATT_y

Cuts at:      0     449     537    1117    1299 
   Size:        449      88     580     182

  Fragments arranged by size: 

                 580     449     182      88
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     541     924    1299 
   Size:        541     383     375

  Fragments arranged by size: 

                 541     383     375
 
BfrBI ATG'CAT

Cuts at:      0    1102    1299 
   Size:       1102     197
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     628    1299 
   Size:        628     671
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     340    1299 
   Size:        340     959
 
BsaWI w'CCGG_w

Cuts at:      0     133    1299 
   Size:        133    1166
 
BsmI GAATG_Cn'

Cuts at:      0    1102    1299 
   Size:       1102     197
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     447    1299 
   Size:        447     852
 
BstBI TT'CG_AA

Cuts at:      0     720    1299 
   Size:        720     579
 
BstYI r'GATC_y

Cuts at:      0     647    1218    1299 
   Size:        647     571      81

  Fragments arranged by size: 

                 647     571      81
 
Bsu36I CC'TnA_GG

Cuts at:      0    1283    1299 
   Size:       1283      16
 
BtsI GCAGTG_nn'

Cuts at:      0     329     828    1299 
   Size:        329     499     471

  Fragments arranged by size: 

                 499     471     329
 
DraI TTT'AAA

Cuts at:      0     995    1299 
   Size:        995     304
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     340     688     842     918    1299 
   Size:        340     348     154      76     381

  Fragments arranged by size: 

                 381     348     340     154      76
 
EcoRI G'AATT_C

Cuts at:      0     449    1299 
   Size:        449     850
 
EcoRV GAT'ATC

Cuts at:      0     485    1299 
   Size:        485     814
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     948    1299 
   Size:        948     351
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     410     442     765     797    1299 
   Size:        410      32     323      32     502

  Fragments arranged by size: 

                 502     410     323      32      32
 
HindIII A'AGCT_T

Cuts at:      0     114    1299 
   Size:        114    1185
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     233    1299 
   Size:        233    1066
 
MslI CAynn'nnrTG

Cuts at:      0      52      85     585    1299 
   Size:         52      33     500     714

  Fragments arranged by size: 

                 714     500      52      33
 
NsiI A_TGCA'T

Cuts at:      0    1104    1299 
   Size:       1104     195
 
NspI r_CATG'y

Cuts at:      0      33     813    1299 
   Size:         33     780     486

  Fragments arranged by size: 

                 780     486      33
 
PciI A'CATG_T

Cuts at:      0     809    1299 
   Size:        809     490
 
SfcI C'TryA_G

Cuts at:      0     296     803    1163    1299 
   Size:        296     507     360     136

  Fragments arranged by size: 

                 507     360     296     136
 
SspI AAT'ATT

Cuts at:      0     656     717    1299 
   Size:        656      61     582

  Fragments arranged by size: 

                 656     582      61
 
TatI w'GTAC_w

Cuts at:      0     255     595     642    1299 
   Size:        255     340      47     657

  Fragments arranged by size: 

                 657     340     255      47
 

 Enzymes that do cut:

     AclI     AcuI   AflIII    AlwNI     ApoI     BbsI    BfrBI     BmrI
     BpmI    BsaWI     BsmI    BspMI    BstBI    BstYI   Bsu36I     BtsI
     DraI  Eco57MI    EcoRI    EcoRV     FalI    Hin4I  HindIII     MmeI
     MslI     NsiI     NspI     PciI     SfcI     SspI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI
 Bsp1286I    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI   BstEII    BstXI  BstZ17I     BtgI    BtgZI     ClaI
    CspCI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
    EcoNI EcoO109I     FseI     FspI    FspAI    HaeII   HincII     HpaI
     KasI     KpnI     MfeI     MluI     MscI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI
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