(Linear) (Six-base) MAPSORT of: KIBB4126.seq Check: 9659 from: 1 to: 1299 KIBB4126.orf With 182 enzymes: SgfI * February 28, 2007 11:25 .. AclI AA'CG_TT Cuts at: 0 395 1299 Size: 395 904 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 688 842 918 1299 Size: 688 154 76 381 Fragments arranged by size: 688 381 154 76 AflIII A'CryG_T Cuts at: 0 809 1299 Size: 809 490 AlwNI CAG_nnn'CTG Cuts at: 0 865 1299 Size: 865 434 ApoI r'AATT_y Cuts at: 0 449 537 1117 1299 Size: 449 88 580 182 Fragments arranged by size: 580 449 182 88 BbsI GAAGACnn'nnnn_ Cuts at: 0 541 924 1299 Size: 541 383 375 Fragments arranged by size: 541 383 375 BfrBI ATG'CAT Cuts at: 0 1102 1299 Size: 1102 197 BmrI ACTGGGnnnn_n' Cuts at: 0 628 1299 Size: 628 671 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 340 1299 Size: 340 959 BsaWI w'CCGG_w Cuts at: 0 133 1299 Size: 133 1166 BsmI GAATG_Cn' Cuts at: 0 1102 1299 Size: 1102 197 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 447 1299 Size: 447 852 BstBI TT'CG_AA Cuts at: 0 720 1299 Size: 720 579 BstYI r'GATC_y Cuts at: 0 647 1218 1299 Size: 647 571 81 Fragments arranged by size: 647 571 81 Bsu36I CC'TnA_GG Cuts at: 0 1283 1299 Size: 1283 16 BtsI GCAGTG_nn' Cuts at: 0 329 828 1299 Size: 329 499 471 Fragments arranged by size: 499 471 329 DraI TTT'AAA Cuts at: 0 995 1299 Size: 995 304 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 340 688 842 918 1299 Size: 340 348 154 76 381 Fragments arranged by size: 381 348 340 154 76 EcoRI G'AATT_C Cuts at: 0 449 1299 Size: 449 850 EcoRV GAT'ATC Cuts at: 0 485 1299 Size: 485 814 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 948 1299 Size: 948 351 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 410 442 765 797 1299 Size: 410 32 323 32 502 Fragments arranged by size: 502 410 323 32 32 HindIII A'AGCT_T Cuts at: 0 114 1299 Size: 114 1185 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 233 1299 Size: 233 1066 MslI CAynn'nnrTG Cuts at: 0 52 85 585 1299 Size: 52 33 500 714 Fragments arranged by size: 714 500 52 33 NsiI A_TGCA'T Cuts at: 0 1104 1299 Size: 1104 195 NspI r_CATG'y Cuts at: 0 33 813 1299 Size: 33 780 486 Fragments arranged by size: 780 486 33 PciI A'CATG_T Cuts at: 0 809 1299 Size: 809 490 SfcI C'TryA_G Cuts at: 0 296 803 1163 1299 Size: 296 507 360 136 Fragments arranged by size: 507 360 296 136 SspI AAT'ATT Cuts at: 0 656 717 1299 Size: 656 61 582 Fragments arranged by size: 656 582 61 TatI w'GTAC_w Cuts at: 0 255 595 642 1299 Size: 255 340 47 657 Fragments arranged by size: 657 340 255 47 Enzymes that do cut: AclI AcuI AflIII AlwNI ApoI BbsI BfrBI BmrI BpmI BsaWI BsmI BspMI BstBI BstYI Bsu36I BtsI DraI Eco57MI EcoRI EcoRV FalI Hin4I HindIII MmeI MslI NsiI NspI PciI SfcI SspI TatI Enzymes that do not cut: AarI AatII AccI Acc65I AfeI AflII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI BstZ17I BtgI BtgZI ClaI CspCI DraIII DrdI EaeI EagI EarI EciI EcoICRI EcoNI EcoO109I FseI FspI FspAI HaeII HincII HpaI KasI KpnI MfeI MluI MscI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PasI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI StyI SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI