Restriction map for
pF1KB4144
(Linear) (Six-base) MAPSORT of: KIBB4144.seq Check: 6690 from: 1 to: 468
KIBB4144.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 128 468
Size: 128 340
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 468
Size: 175 293
AleI CACnn'nnGTG
Cuts at: 0 445 468
Size: 445 23
AlwNI CAG_nnn'CTG
Cuts at: 0 120 468
Size: 120 348
AvaI C'yCGr_G
Cuts at: 0 365 468
Size: 365 103
BanI G'GyrC_C
Cuts at: 0 378 468
Size: 378 90
BanII G_rGCy'C
Cuts at: 0 434 468
Size: 434 34
BbeI G_GCGC'C
Cuts at: 0 382 468
Size: 382 86
BlpI GC'TnA_GC
Cuts at: 0 70 468
Size: 70 398
Bme1580I G_kGCm'C
Cuts at: 0 452 468
Size: 452 16
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 453 468
Size: 453 15
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 325 468
Size: 256 69 143
Fragments arranged by size:
256 143 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 468
Size: 160 308
BsaI GGTCTCn'nnnn_
Cuts at: 0 133 468
Size: 133 335
BsaHI Gr'CG_yC
Cuts at: 0 314 379 468
Size: 314 65 89
Fragments arranged by size:
314 89 65
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 154 244 426 468
Size: 154 90 182 42
Fragments arranged by size:
182 154 90 42
BsiHKAI G_wGCw'C
Cuts at: 0 71 434 468
Size: 71 363 34
Fragments arranged by size:
363 71 34
Bsp1286I G_dGCh'C
Cuts at: 0 71 434 452 468
Size: 71 363 18 16
Fragments arranged by size:
363 71 18 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 128 468
Size: 128 340
BsrBI CCG'CTC
Cuts at: 0 240 468
Size: 240 228
BssHII G'CGCG_C
Cuts at: 0 352 468
Size: 352 116
BstEII G'GTnAC_C
Cuts at: 0 144 468
Size: 144 324
BtgI C'CryG_G
Cuts at: 0 183 359 468
Size: 183 176 109
Fragments arranged by size:
183 176 109
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 89 468
Size: 89 379
EaeI y'GGCC_r
Cuts at: 0 194 392 468
Size: 194 198 76
Fragments arranged by size:
198 194 76
EcoICRI GAG'CTC
Cuts at: 0 432 468
Size: 432 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 256 325 468
Size: 175 81 69 143
Fragments arranged by size:
175 143 81 69
EcoO109I rG'GnC_Cy
Cuts at: 0 370 468
Size: 370 98
HaeII r_GCGC'y
Cuts at: 0 340 382 468
Size: 340 42 86
Fragments arranged by size:
340 86 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 26 73 105 468
Size: 26 47 32 363
Fragments arranged by size:
363 47 32 26
KasI G'GCGC_C
Cuts at: 0 378 468
Size: 378 90
MscI TGG'CCA
Cuts at: 0 394 468
Size: 394 74
MslI CAynn'nnrTG
Cuts at: 0 97 445 468
Size: 97 348 23
Fragments arranged by size:
348 97 23
MspA1I CmG'CkG
Cuts at: 0 185 200 468
Size: 185 15 268
Fragments arranged by size:
268 185 15
NarI GG'CG_CC
Cuts at: 0 379 468
Size: 379 89
NspI r_CATG'y
Cuts at: 0 300 468
Size: 300 168
PpuMI rG'GwC_Cy
Cuts at: 0 370 468
Size: 370 98
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 172 204 468
Size: 172 32 264
Fragments arranged by size:
264 172 32
PstI C_TGCA'G
Cuts at: 0 260 344 468
Size: 260 84 124
Fragments arranged by size:
260 124 84
PvuII CAG'CTG
Cuts at: 0 200 468
Size: 200 268
SacI G_AGCT'C
Cuts at: 0 434 468
Size: 434 34
SacII CC_GC'GG
Cuts at: 0 186 468
Size: 186 282
SfcI C'TryA_G
Cuts at: 0 256 340 468
Size: 256 84 128
Fragments arranged by size:
256 128 84
SfoI GGC'GCC
Cuts at: 0 380 468
Size: 380 88
SmaI CCC'GGG
Cuts at: 0 367 468
Size: 367 101
SmlI C'TyrA_G
Cuts at: 0 175 468
Size: 175 293
SphI G_CATG'C
Cuts at: 0 300 468
Size: 300 168
StyI C'CwwG_G
Cuts at: 0 134 386 468
Size: 134 252 82
Fragments arranged by size:
252 134 82
XmaI C'CCGG_G
Cuts at: 0 365 468
Size: 365 103
Enzymes that do cut:
AarI AcuI AleI AlwNI AvaI BanI BanII BbeI
BlpI Bme1580I BplI BpmI BpuEI BsaI BsaHI BseRI
BsiHKAI Bsp1286I BspMI BsrBI BssHII BstEII BtgI BtgZI
EaeI EcoICRI Eco57MI EcoO109I HaeII Hin4I KasI MscI
MslI MspA1I NarI NspI PpuMI PsrI PstI PvuII
SacI SacII SfcI SfoI SmaI SmlI SphI StyI
XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BmgBI BmrI BmtI Bpu10I
BsaAI BsaBI BsaWI BsaXI BseYI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtsI ClaI
CspCI DraI DraIII DrdI EagI EarI EciI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI MmeI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PvuI RsrII SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI
StuI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmnI ZraI