Restriction map for
pF1KB4148
(Linear) (Six-base) MAPSORT of: KIBB4148.seq Check: 3213 from: 1 to: 1572
KIBB4148.orf
With 182 enzymes: SgfI *
January 9, 2009 11:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 357 699 1572
Size: 357 342 873
Fragments arranged by size:
873 357 342
AccI GT'mk_AC
Cuts at: 0 968 1572
Size: 968 604
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 184 454 755 1184 1572
Size: 140 44 270 301 429 388
Fragments arranged by size:
429 388 301 270 140 44
AlwNI CAG_nnn'CTG
Cuts at: 0 1356 1572
Size: 1356 216
ApoI r'AATT_y
Cuts at: 0 826 1223 1572
Size: 826 397 349
Fragments arranged by size:
826 397 349
AseI AT'TA_AT
Cuts at: 0 931 1572
Size: 931 641
AvaI C'yCGr_G
Cuts at: 0 20 61 1572
Size: 20 41 1511
Fragments arranged by size:
1511 41 20
BanI G'GyrC_C
Cuts at: 0 948 1572
Size: 948 624
BanII G_rGCy'C
Cuts at: 0 867 1572
Size: 867 705
BbsI GAAGACnn'nnnn_
Cuts at: 0 1065 1178 1245 1572
Size: 1065 113 67 327
Fragments arranged by size:
1065 327 113 67
BciVI GTATCCnnnnn_n'
Cuts at: 0 137 1572
Size: 137 1435
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1361 1572
Size: 1361 211
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 860 1279 1572
Size: 860 419 293
Fragments arranged by size:
860 419 293
BsaAI yAC'GTr
Cuts at: 0 1079 1572
Size: 1079 493
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1091 1121 1572
Size: 1091 30 451
Fragments arranged by size:
1091 451 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1285 1572
Size: 1285 287
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 11 575 1572
Size: 11 564 997
Fragments arranged by size:
997 564 11
BsiHKAI G_wGCw'C
Cuts at: 0 867 1572
Size: 867 705
BsmI GAATG_Cn'
Cuts at: 0 487 1478 1572
Size: 487 991 94
Fragments arranged by size:
991 487 94
Bsp1286I G_dGCh'C
Cuts at: 0 867 1572
Size: 867 705
BspHI T'CATG_A
Cuts at: 0 867 1572
Size: 867 705
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 357 699 1572
Size: 357 342 873
Fragments arranged by size:
873 357 342
BsrFI r'CCGG_y
Cuts at: 0 15 1572
Size: 15 1557
BsrGI T'GTAC_A
Cuts at: 0 896 1494 1572
Size: 896 598 78
Fragments arranged by size:
896 598 78
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1356 1572
Size: 1356 216
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1183 1572
Size: 1183 389
BtgI C'CryG_G
Cuts at: 0 300 1545 1572
Size: 300 1245 27
Fragments arranged by size:
1245 300 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 91 1572
Size: 91 1481
BtsI GCAGTG_nn'
Cuts at: 0 824 1572
Size: 824 748
ClaI AT'CG_AT
Cuts at: 0 853 1572
Size: 853 719
DraI TTT'AAA
Cuts at: 0 599 1572
Size: 599 973
DraIII CAC_nnn'GTG
Cuts at: 0 177 287 1572
Size: 177 110 1285
Fragments arranged by size:
1285 177 110
DrdI GACnn_nn'nnGTC
Cuts at: 0 76 1572
Size: 76 1496
EaeI y'GGCC_r
Cuts at: 0 2 303 1572
Size: 2 301 1269
Fragments arranged by size:
1269 301 2
EarI CTCTTCn'nnn_
Cuts at: 0 1433 1572
Size: 1433 139
EcoICRI GAG'CTC
Cuts at: 0 865 1572
Size: 865 707
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 184 454 755 1184 1361 1572
Size: 140 44 270 301 429 177 211
Fragments arranged by size:
429 301 270 211 177 140 44
EcoO109I rG'GnC_Cy
Cuts at: 0 1299 1572
Size: 1299 273
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 189 1572
Size: 189 1383
HindIII A'AGCT_T
Cuts at: 0 554 566 1572
Size: 554 12 1006
Fragments arranged by size:
1006 554 12
MfeI C'AATT_G
Cuts at: 0 1232 1572
Size: 1232 340
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1077 1572
Size: 1077 495
MscI TGG'CCA
Cuts at: 0 305 1572
Size: 305 1267
MslI CAynn'nnrTG
Cuts at: 0 1315 1572
Size: 1315 257
MspA1I CmG'CkG
Cuts at: 0 1192 1572
Size: 1192 380
NaeI GCC'GGC
Cuts at: 0 17 1572
Size: 17 1555
NcoI C'CATG_G
Cuts at: 0 300 1572
Size: 300 1272
NgoMIV G'CCGG_C
Cuts at: 0 15 1572
Size: 15 1557
NspI r_CATG'y
Cuts at: 0 1534 1572
Size: 1534 38
PasI CC'CwG_GG
Cuts at: 0 149 1572
Size: 149 1423
PmlI CAC'GTG
Cuts at: 0 1079 1572
Size: 1079 493
PsiI TTA'TAA
Cuts at: 0 806 1572
Size: 806 766
PstI C_TGCA'G
Cuts at: 0 1197 1432 1572
Size: 1197 235 140
Fragments arranged by size:
1197 235 140
PvuII CAG'CTG
Cuts at: 0 1192 1572
Size: 1192 380
SacI G_AGCT'C
Cuts at: 0 867 1572
Size: 867 705
SfcI C'TryA_G
Cuts at: 0 888 914 1193 1428 1572
Size: 888 26 279 235 144
Fragments arranged by size:
888 279 235 144 26
SmaI CCC'GGG
Cuts at: 0 22 63 1572
Size: 22 41 1509
Fragments arranged by size:
1509 41 22
SmlI C'TyrA_G
Cuts at: 0 875 1294 1572
Size: 875 419 278
Fragments arranged by size:
875 419 278
SrfI GCCC'GGGC
Cuts at: 0 22 1572
Size: 22 1550
SspI AAT'ATT
Cuts at: 0 502 1017 1572
Size: 502 515 555
Fragments arranged by size:
555 515 502
StyI C'CwwG_G
Cuts at: 0 300 1572
Size: 300 1272
TatI w'GTAC_w
Cuts at: 0 896 1494 1572
Size: 896 598 78
Fragments arranged by size:
896 598 78
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1091 1123 1572
Size: 1091 32 449
Fragments arranged by size:
1091 449 32
Tth111I GACn'n_nGTC
Cuts at: 0 73 1572
Size: 73 1499
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1046 1572
Size: 1046 526
XmaI C'CCGG_G
Cuts at: 0 20 61 1572
Size: 20 41 1511
Fragments arranged by size:
1511 41 20
Enzymes that do cut:
AarI AccI AcuI AlwNI ApoI AseI AvaI BanI
BanII BbsI BciVI BpmI BpuEI BsaAI BsaXI BseRI
BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrFI BsrGI
BstAPI BstXI BtgI BtgZI BtsI ClaI DraI DraIII
DrdI EaeI EarI EcoICRI Eco57MI EcoO109I FalI HindIII
MfeI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV
NspI PasI PmlI PsiI PstI PvuII SacI SfcI
SmaI SmlI SrfI SspI StyI TatI TstI Tth111I
XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI Bpu10I
BsaI BsaBI BsaHI BsaWI BseYI BsiEI BsiWI BsmBI
BspEI BsrBI BsrDI BssHII BssSI BstBI BstEII BstYI
BstZ17I Bsu36I CspCI EagI EciI EcoNI EcoRI EcoRV
FseI FspI FspAI HaeII Hin4I HincII HpaI KasI
KpnI MluI NarI NdeI NheI NotI NruI NsiI
PacI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI StuI SwaI TaqII XbaI XhoI
XmnI ZraI