(Linear) (Six-base) MAPSORT of: KIBB4150.seq  Check: 4339  from: 1  to: 381



KIBB4150.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:25  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     296     358     381 
   Size:        296      62      23

  Fragments arranged by size: 

                 296      62      23
 
AflIII A'CryG_T

Cuts at:      0     185     381 
   Size:        185     196
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     201     234     381 
   Size:        201      33     147

  Fragments arranged by size: 

                 201     147      33
 
BamHI G'GATC_C

Cuts at:      0     270     381 
   Size:        270     111
 
BanII G_rGCy'C

Cuts at:      0     144     381 
   Size:        144     237
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      80     381 
   Size:         80     301
 
Bsp1286I G_dGCh'C

Cuts at:      0     144     381 
   Size:        144     237
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     326     381 
   Size:        326      55
 
BstYI r'GATC_y

Cuts at:      0     270     381 
   Size:        270     111
 
BtsI GCAGTG_nn'

Cuts at:      0      48     381 
   Size:         48     333
 
DraI TTT'AAA

Cuts at:      0     231     314     381 
   Size:        231      83      67

  Fragments arranged by size: 

                 231      83      67
 
EarI CTCTTCn'nnn_

Cuts at:      0      39     381 
   Size:         39     342
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      80     296     358     381 
   Size:         80     216      62      23

  Fragments arranged by size: 

                 216      80      62      23
 
HincII GTy'rAC

Cuts at:      0      63     381 
   Size:         63     318
 
MspA1I CmG'CkG

Cuts at:      0     336     381 
   Size:        336      45
 
NruI TCG'CGA

Cuts at:      0     224     381 
   Size:        224     157
 
NspI r_CATG'y

Cuts at:      0     189     381 
   Size:        189     192
 
PciI A'CATG_T

Cuts at:      0     185     381 
   Size:        185     196
 
PvuII CAG'CTG

Cuts at:      0     336     381 
   Size:        336      45
 
SapI GCTCTTCn'nnn_

Cuts at:      0      39     381 
   Size:         39     342
 
SspI AAT'ATT

Cuts at:      0     262     381 
   Size:        262     119
 

 Enzymes that do cut:

     AcuI   AflIII     BaeI    BamHI    BanII     BpmI Bsp1286I    BstXI
    BstYI     BtsI     DraI     EarI  Eco57MI   HincII   MspA1I     NruI
     NspI     PciI    PvuII     SapI     SspI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NsiI
     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     StuI     StyI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI