Restriction map for
pF1KB4151
(Linear) (Six-base) MAPSORT of: KIBB4151.seq Check: 8411 from: 1 to: 3174
KIBB4151.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1812 3174
Size: 1812 1362
AccI GT'mk_AC
Cuts at: 0 1766 3174
Size: 1766 1408
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1921 3174
Size: 1921 1253
AleI CACnn'nnGTG
Cuts at: 0 542 3174
Size: 542 2632
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2101 3174
Size: 2101 1073
AlwNI CAG_nnn'CTG
Cuts at: 0 937 1005 2175 2439 3174
Size: 937 68 1170 264 735
Fragments arranged by size:
1170 937 735 264 68
ApaI G_GGCC'C
Cuts at: 0 2234 3174
Size: 2234 940
ApoI r'AATT_y
Cuts at: 0 2392 3174
Size: 2392 782
AvaI C'yCGr_G
Cuts at: 0 1100 1943 3174
Size: 1100 843 1231
Fragments arranged by size:
1231 1100 843
BanI G'GyrC_C
Cuts at: 0 105 1182 1804 2744 3174
Size: 105 1077 622 940 430
Fragments arranged by size:
1077 940 622 430 105
BanII G_rGCy'C
Cuts at: 0 413 1107 1944 2234 2486 3174
Size: 413 694 837 290 252 688
Fragments arranged by size:
837 694 688 413 290 252
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1924 1958 3174
Size: 1924 34 1216
Fragments arranged by size:
1924 1216 34
BglI GCCn_nnn'nGGC
Cuts at: 0 242 325 928 1041 3174
Size: 242 83 603 113 2133
Fragments arranged by size:
2133 603 242 113 83
BlpI GC'TnA_GC
Cuts at: 0 407 1022 3174
Size: 407 615 2152
Fragments arranged by size:
2152 615 407
Bme1580I G_kGCm'C
Cuts at: 0 101 316 1807 2234 2747 3174
Size: 101 215 1491 427 513 427
Fragments arranged by size:
1491 513 427 427 215 101
BmgBI CAC'GTC
Cuts at: 0 2830 3174
Size: 2830 344
BmrI ACTGGGnnnn_n'
Cuts at: 0 72 315 336 1810 2142 3174
Size: 72 243 21 1474 332 1032
Fragments arranged by size:
1474 1032 332 243 72 21
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 526 1318 1807 2083 2740 3174
Size: 193 333 792 489 276 657 434
Fragments arranged by size:
792 657 489 434 333 276 193
Bpu10I CC'TnA_GC
Cuts at: 0 1080 2208 3174
Size: 1080 1128 966
Fragments arranged by size:
1128 1080 966
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1306 1435 3004 3127 3174
Size: 1306 129 1569 123 47
Fragments arranged by size:
1569 1306 129 123 47
BsaAI yAC'GTr
Cuts at: 0 3090 3174
Size: 3090 84
BsaHI Gr'CG_yC
Cuts at: 0 2411 3174
Size: 2411 763
BsaWI w'CCGG_w
Cuts at: 0 1148 1772 3174
Size: 1148 624 1402
Fragments arranged by size:
1402 1148 624
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 727 757 857 887 3174
Size: 727 30 100 30 2287
Fragments arranged by size:
2287 727 100 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 373 1069 2494 2917 3174
Size: 373 696 1425 423 257
Fragments arranged by size:
1425 696 423 373 257
BseYI C'CCAG_C
Cuts at: 0 980 1171 1395 1473 2500 2673 3078 3174
Size: 980 191 224 78 1027 173 405 96
Fragments arranged by size:
1027 980 405 224 191 173 96 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 970 991 1138 1840 2092 2161 2401 2758
Size: 970 21 147 702 252 69 240 357
Cuts at: 2758 3174
Size: 416
Fragments arranged by size:
970 702 416 357 252 240 147 69
21
BsiHKAI G_wGCw'C
Cuts at: 0 413 491 1023 1928 2486 2948 3174
Size: 413 78 532 905 558 462 226
Fragments arranged by size:
905 558 532 462 413 226 78
BsmI GAATG_Cn'
Cuts at: 0 1101 3174
Size: 1101 2073
Bsp1286I G_dGCh'C
Cuts at: 0 101 316 413 491 1023 1107 1807 1928
Size: 101 215 97 78 532 84 700 121
Cuts at: 1928 1944 2234 2486 2747 2948 3174
Size: 16 290 252 261 201 226
Fragments arranged by size:
700 532 290 261 252 226 215 201
121 101 97 84 78 16
BspEI T'CCGG_A
Cuts at: 0 1148 3174
Size: 1148 2026
BspHI T'CATG_A
Cuts at: 0 1226 3174
Size: 1226 1948
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 359 706 1391 1812 2373 3174
Size: 359 347 685 421 561 801
Fragments arranged by size:
801 685 561 421 359 347
BsrBI CCG'CTC
Cuts at: 0 731 2253 3131 3174
Size: 731 1522 878 43
Fragments arranged by size:
1522 878 731 43
BsrDI GCAATG_nn'
Cuts at: 0 203 1056 1821 2678 3174
Size: 203 853 765 857 496
Fragments arranged by size:
857 853 765 496 203
BsrFI r'CCGG_y
Cuts at: 0 1031 2636 3174
Size: 1031 1605 538
Fragments arranged by size:
1605 1031 538
BsrGI T'GTAC_A
Cuts at: 0 1745 2726 3174
Size: 1745 981 448
Fragments arranged by size:
1745 981 448
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1932 2175 2379 3174
Size: 1932 243 204 795
Fragments arranged by size:
1932 795 243 204
BstEII G'GTnAC_C
Cuts at: 0 669 693 3174
Size: 669 24 2481
Fragments arranged by size:
2481 669 24
BstXI CCAn_nnnn'nTGG
Cuts at: 0 120 225 1722 2016 2105 3174
Size: 120 105 1497 294 89 1069
Fragments arranged by size:
1497 1069 294 120 105 89
BstYI r'GATC_y
Cuts at: 0 1176 2417 3174
Size: 1176 1241 757
Fragments arranged by size:
1241 1176 757
Bsu36I CC'TnA_GG
Cuts at: 0 1298 3174
Size: 1298 1876
BtgI C'CryG_G
Cuts at: 0 543 791 861 2699 3174
Size: 543 248 70 1838 475
Fragments arranged by size:
1838 543 475 248 70
BtsI GCAGTG_nn'
Cuts at: 0 141 314 431 1121 1270 1780 1942 2026
Size: 141 173 117 690 149 510 162 84
Cuts at: 2026 3174
Size: 1148
Fragments arranged by size:
1148 690 510 173 162 149 141 117
84
DraI TTT'AAA
Cuts at: 0 909 3174
Size: 909 2265
DraIII CAC_nnn'GTG
Cuts at: 0 652 3174
Size: 652 2522
DrdI GACnn_nn'nnGTC
Cuts at: 0 1309 3174
Size: 1309 1865
EaeI y'GGCC_r
Cuts at: 0 122 1029 1033 1712 2360 3174
Size: 122 907 4 679 648 814
Fragments arranged by size:
907 814 679 648 122 4
EarI CTCTTCn'nnn_
Cuts at: 0 598 1969 3174
Size: 598 1371 1205
Fragments arranged by size:
1371 1205 598
EcoICRI GAG'CTC
Cuts at: 0 411 2484 3174
Size: 411 2073 690
Fragments arranged by size:
2073 690 411
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 526 1318 1807 1921 2083 2740 3174
Size: 193 333 792 489 114 162 657 434
Fragments arranged by size:
792 657 489 434 333 193 162 114
EcoNI CCTnn'n_nnAGG
Cuts at: 0 355 454 706 1135 1318 3174
Size: 355 99 252 429 183 1856
Fragments arranged by size:
1856 429 355 252 183 99
EcoO109I rG'GnC_Cy
Cuts at: 0 156 243 576 811 1002 1140 1206 1311
Size: 156 87 333 235 191 138 66 105
Cuts at: 1311 1657 1866 2385 3174
Size: 346 209 519 789
Fragments arranged by size:
789 519 346 333 235 209 191 156
138 105 87 66
EcoRI G'AATT_C
Cuts at: 0 2392 3174
Size: 2392 782
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 921 3174
Size: 921 2253
FseI GG_CCGG'CC
Cuts at: 0 1035 3174
Size: 1035 2139
HaeII r_GCGC'y
Cuts at: 0 1341 2218 2450 3100 3174
Size: 1341 877 232 650 74
Fragments arranged by size:
1341 877 650 232 74
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 322 354 727 759 857 889 1484 1516
Size: 322 32 373 32 98 32 595 32
Cuts at: 1516 2325 2357 3152 3174
Size: 809 32 795 22
Fragments arranged by size:
809 795 595 373 322 98 32 32
32 32 32 22
HincII GTy'rAC
Cuts at: 0 1989 2268 3174
Size: 1989 279 906
Fragments arranged by size:
1989 906 279
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 866 875 2621 3011 3174
Size: 866 9 1746 390 163
Fragments arranged by size:
1746 866 390 163 9
MscI TGG'CCA
Cuts at: 0 124 1714 2362 3174
Size: 124 1590 648 812
Fragments arranged by size:
1590 812 648 124
MslI CAynn'nnrTG
Cuts at: 0 542 1747 3174
Size: 542 1205 1427
Fragments arranged by size:
1427 1205 542
MspA1I CmG'CkG
Cuts at: 0 15 174 516 984 1603 1760 2172 3010
Size: 15 159 342 468 619 157 412 838
Cuts at: 3010 3174
Size: 164
Fragments arranged by size:
838 619 468 412 342 164 159 157
15
NaeI GCC'GGC
Cuts at: 0 1033 3174
Size: 1033 2141
NcoI C'CATG_G
Cuts at: 0 791 3174
Size: 791 2383
NdeI CA'TA_TG
Cuts at: 0 1161 2573 3174
Size: 1161 1412 601
Fragments arranged by size:
1412 1161 601
NgoMIV G'CCGG_C
Cuts at: 0 1031 3174
Size: 1031 2143
NspI r_CATG'y
Cuts at: 0 1746 3174
Size: 1746 1428
PasI CC'CwG_GG
Cuts at: 0 1562 2973 3174
Size: 1562 1411 201
Fragments arranged by size:
1562 1411 201
PflMI CCAn_nnn'nTGG
Cuts at: 0 483 3174
Size: 483 2691
PfoI T'CCnGG_A
Cuts at: 0 1240 3174
Size: 1240 1934
PmlI CAC'GTG
Cuts at: 0 3090 3174
Size: 3090 84
PpuMI rG'GwC_Cy
Cuts at: 0 811 1140 1311 1657 1866 2385 3174
Size: 811 329 171 346 209 519 789
Fragments arranged by size:
811 789 519 346 329 209 171
PshAI GACnn'nnGTC
Cuts at: 0 2471 3174
Size: 2471 703
PspOMI G'GGCC_C
Cuts at: 0 2230 3174
Size: 2230 944
PspXI vC'TCGA_Gb
Cuts at: 0 1100 3174
Size: 1100 2074
PstI C_TGCA'G
Cuts at: 0 1602 1937 2093 2375 2444 2634 2891 3174
Size: 1602 335 156 282 69 190 257 283
Fragments arranged by size:
1602 335 283 282 257 190 156 69
PvuII CAG'CTG
Cuts at: 0 174 516 984 1603 2172 3010 3174
Size: 174 342 468 619 569 838 164
Fragments arranged by size:
838 619 569 468 342 174 164
SacI G_AGCT'C
Cuts at: 0 413 2486 3174
Size: 413 2073 688
Fragments arranged by size:
2073 688 413
SapI GCTCTTCn'nnn_
Cuts at: 0 598 3174
Size: 598 2576
ScaI AGT'ACT
Cuts at: 0 2152 2839 3174
Size: 2152 687 335
Fragments arranged by size:
2152 687 335
SfcI C'TryA_G
Cuts at: 0 1598 1933 2089 2371 2440 2630 2887 3174
Size: 1598 335 156 282 69 190 257 287
Fragments arranged by size:
1598 335 287 282 257 190 156 69
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 242 1041 3174
Size: 242 799 2133
Fragments arranged by size:
2133 799 242
SmlI C'TyrA_G
Cuts at: 0 1100 1285 1450 3019 3106 3174
Size: 1100 185 165 1569 87 68
Fragments arranged by size:
1569 1100 185 165 87 68
StuI AGG'CCT
Cuts at: 0 236 1234 1961 3174
Size: 236 998 727 1213
Fragments arranged by size:
1213 998 727 236
StyI C'CwwG_G
Cuts at: 0 276 674 791 914 2051 3174
Size: 276 398 117 123 1137 1123
Fragments arranged by size:
1137 1123 398 276 123 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 514 1200 1411 2161 2363 3174
Size: 514 686 211 750 202 811
Fragments arranged by size:
811 750 686 514 211 202
TatI w'GTAC_w
Cuts at: 0 756 1745 2150 2726 2837 3174
Size: 756 989 405 576 111 337
Fragments arranged by size:
989 756 576 405 337 111
Tth111I GACn'n_nGTC
Cuts at: 0 739 1845 3174
Size: 739 1106 1329
Fragments arranged by size:
1329 1106 739
XbaI T'CTAG_A
Cuts at: 0 2475 3174
Size: 2475 699
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 501 988 1470 1702 2697 3174
Size: 501 487 482 232 995 477
Fragments arranged by size:
995 501 487 482 477 232
XhoI C'TCGA_G
Cuts at: 0 1100 3174
Size: 1100 2074
XmnI GAAnn'nnTTC
Cuts at: 0 1411 1906 2773 3174
Size: 1411 495 867 401
Fragments arranged by size:
1411 867 495 401
Enzymes that do cut:
AarI AccI AcuI AleI AlfI AlwNI ApaI ApoI
AvaI BanI BanII BcgI BglI BlpI Bme1580I BmgBI
BmrI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtsI DraI DraIII DrdI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
HaeII Hin4I HincII MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NspI PasI PflMI PfoI PmlI
PpuMI PshAI PspOMI PspXI PstI PvuII SacI SapI
ScaI SfcI SfiI SmlI StuI StyI TaqII TatI
Tth111I XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbsI BbvCI BciVI BclI BfrBI BglII BmtI
BplI BsaI BsaBI BsiEI BsiWI BsmBI BssHII BssSI
BstBI BstZ17I BtgZI ClaI CspCI EagI EciI EcoRV
FspI FspAI HindIII HpaI KasI KpnI MfeI MluI
NarI NheI NotI NruI NsiI PacI PciI PmeI
PpiI PsiI PsrI PvuI RsrII SacII SalI SanDI
SbfI SexAI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TstI XmaI ZraI